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PENGARUH JARAK DAN LAMA WAKTU PROSES PENGHITUNGAN STRAW SEBELUM DISTRIBUSI TERHADAP KUALITAS SEMEN BEKU SAPI SIMENTAL POST THAWING Siti Nur Fatimah; Wurlina Wurlina; Retno Sri Wahjuni
Ovozoa : Journal of Animal Reproduction Vol. 7 No. 2 (2018): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (503.914 KB) | DOI: 10.20473/ovz.v7i2.2018.126-130

Abstract

Aims of this research was to determine the effect of distance and the duration of straw count above liquid nitrogen to the Quality of Frozen Simental Cattle. This study used a sample derived from simmental cattle from BBIB singosari as much as 48 straw divided into 8 treatments. P1(0,5cm 15 sec), P2(0,5cm 30 sec), P3( 0,5cm 45 sec), P4(0,5cm 60 sec), P5(1,5cm 15 sec), P6(1,5cm 30 sec), P7(1,5cm 45 sec), P8(1,5cm 60 sec) and repeated as much as 6 times then observed for motility and viability of post thawing spermatozoa. The data obtained were analyzed using SPSS 16.0. The data obtained were analyzed using SPSS 16.0. The results showed that the duration of straw count and the position of straw from liquid nitrogen  not significant (P>0,05) on post thawing motility. The position of straw from liquid nitrogen not signifant (P>0,05) on post thawing viability. The conclusion of this research showed that 0,5 cm 15 sec give the higher post thawing motility.
Efektivitas pemberian GnRH pada sapi perah yang mengalami hipofungsi ovarium terhadap waktu timbulnya birahi dan angka kebuntingan Winadya Reika Ummaisyah; Sri Pantja Madyawati; Rimayanti Rimayanti; Wurlina Wurlina; Tjuk Imam Restiadi; Retno Sri Wahjuni
Ovozoa : Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.64-68

Abstract

Ovarian hypofunction is a condition in which the follicles in the ovary can not develop due to lack of feed. It can affect the pituitary anterior, so the production of FSH and LH was low. GnRH can stimulate the pituitary anterior to produced FSH and LH. This study aimed to prove that GnRH injection in dairy cows with ovarian hypofunction can initiate estrus, followed by pregnancy. This study used 12 dairy cows with BCS ≥ 2.75, with anestrus due to ovarian hypofunction. All dairy cows were divided into two treatment groups, P1 injected with GnRH at a dose of 100 µg intramuscularly and P2 injected with GnRH at a dose of 300 µg intramuscularly. The results were analyzed using an independent t-test and Chi-Square test. The result showed that estrus rate of P1 and P2 were both 100%, meanwhile onset of estrus of P2 (53.83 ± 8.1 h) was shorter p <0.05) than P1 (74.17 ± 4.7 h). Both of P1 and P2 showed 100% pregnancy rate. It could be concluded that GnRH injection could initiate 100% estrus, and followed by 100% pregnancy in dairy cows with ovarian hypofunction.
EFFECTIVENESS MENIRAN ( Phyllanthus niruri Linn ) AS IMMUNOMODULATION ON LYMPHOCYTE OF BROILER INFECTED WITH ENTEROTOXIN ESCHERICHIA COLI RESISTANT ANTIBIOTICS Retno Sri Wahjuni
Jurnal Vokasi Indonesia Vol 5, No 2 (2017): July - December
Publisher : Program Pendidikan Vokasi Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.458 KB) | DOI: 10.7454/jvi.v5i2.87

Abstract

Enterotoxin Escherichia coli resistant to antibiotics on broiler. The used of antibiotics should be re-evaluated, as well as over Escherichia coli enterotoxin. Plants meniran (Phyllanthus niruri Linn) is a plant that can be used as an alternative prevention and treatment of diseases caused by Escherichia coli enterotoxin. In this research, broilers at the age of 23 days were divided into six groups. Two groups are positive control was infected by Escherichia coli and negative control was not infected by Escherichia coli. Three groups are extract of Phyllanthus niruri Linn. plant that variety in three dosage: 20%, 25%, 30% and antibiotic. After five days treatment the broiler was to take the sample of blood tissue. The data were tested with Annova and continued by Duncan multiple range test. There was a significant difference of immunomodulation activity between treatment group doses 30% has been able to decrease lymphocyte on blood. The result showed doses 30% can be used as immunomodulation to Escherichia coli.Keywords: Phyllanthus niruri Linn. extract, Immunomodulation, Escherichia coli, Broiler, Lymphocyte
Anthelmintic Efficacy of Ethanol Extract of Raw Kepok Banana Peel (Musa paradisiaca L.) Against Ascaridia galli Worms In Vitro Annastasya Yuliani Rahayu; Retno Sri Wahjuni; Dewa Ketut Meles; Retno Wulansari
Journal of Parasite Science (JoPS) Vol. 4 No. 2 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v4i2.29154

Abstract

The aims of this study was to know anthelmintic efficacy through determine the concentration, duration, and interaction between concentration and duration of raw kepok banana peel ethanol extract against mortality rate of Ascaridia galli using in vitro method. Present study was used completely randomized design. This method of this study was used a total of 600 samples of Ascaridia galli with length of 7-11 cm without differentiating their sex. The concentration of raw kepok banana peel ethanol extract was divided into 25%, 50% and 75%. Physiological saline solution and piperazine citrate 10 mg/ml were being used as negative and positive control. Each treatment then being replicated six times. The recording of mortality rate was done at 2, 4, 6, 8 and 10 hours of observation. Ascaridia galli was confirmed death if there was no movement while triggered by anatomy tweezers and dipped in slightly warm water (50 0C). The data obtained was analyzed using ANOVA factorial and continued with Duncan Multiple Range Test by SPSS 26 for Windows. The results were showed that in 75% concentration and 10 hours of treatment can cause the highest mortality rate against Ascaridia galli. Interaction between concentration and duration of treatment reveals that 75% concentration of raw kepok banana peel ethanol extract in 10 hours of observation was recorded causing the highest mortality rate against Ascaridia galli.
Detection of Mycotoxigenic Fungi on Food from Markets with the Polymerase Chain Reaction Method for Halal and Thoyiban Food Mohammad Sukmanadi; Retno Sri Wahjuni; Kadek Rachmawati
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14813

Abstract

Secondary metabolites of filamentous mold are mycotoxins, which in some situations can develop on foodsderived from plants or from animals. Fusarium, Aspergillus, and Penicillium are the most common types ofmold that produce mycotoxins and also often contaminate human food and animal feed. Aflatoxins includingaflatoxin B1, B2, G1 and G2 are produced by A. flavus and A. parasiticus. M1 and M2 aflatoxins arefound in dairy products. In this study, we used PCR to detect and identify mycotoxigenic fungi material infoods from traditional markets and supermarkets in Surabaya, Indonesia. Samples of chicken meat from atraditional market and from a supermarket (10 pieces each) were placed in a conical tube and crushed in PBS.The crushed samples were centrifuged and the supernatants were cultured on potato dextrose agar (PDA)media and observed using a reverse microscope. DNA was isolated from cultured samples and subjectedto PCR with primers specific for genes encoding aflatoxins. Interestingly, we revealed that the PolymeraseChain Reaction (PCR) analysis showed A. flavus and A. ochraceus were present on chicken meat sold attraditional markets and supermarkets. In sum, enhanced precautions may be needed to ensure that foods soldin traditional markets and supermarkets are free from molds that have the potential to produce mycotoxins.Further studies are needed to detect and identify the prevalence of mycotoxins in the food supply.
PENGARUH EKSTRAK DELIMA MERAH(Punicagranatum L.) TERHADAP PENINGKATAN PERSENTASE KAPASITASI DAN REAKSI AKROSOM SPERMATOZOA TIKUS PUTIH (Rattusnorvegicus) Baktiar Budi Satrio; Mas’ud Hariadi; Retno Sri Wahjuni; Budi Utomo; Sri Agus Sudjarwo; Tita Damayanti L
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.301 KB) | DOI: 10.20473/ovz.v8i2.2019.100-104

Abstract

The aim of this research is to know the effect of pomegranate (Punicagranatum L.) on the improvement of spermatozoa quality that is increase of percentage of capacitation and acrosome reaction in white rat (Rattusnorvegicus). This research used 20 healthy white rat (Rattusnorvegicus) with average age 2-3 months old, 200-300 g. Research design using completely randomized design (CRD) with four treatments and 5 replications. Rats were randomly divided into 4 treatment groups. Rat in the negative control group not treated just kept alone, solvent control group were given CMC Na 0,5% and is exposed to heat, the treatment groups 1, 2, and 3 induced pomegranate extract standardized to 40% ellagic acid at a dose of 75mg / kg, 150mg / kg and 300mg /kg perorally with doses 1 ml/rat/day gastric for along 14 days and exposed to heat. Data were analyzed using One Way Analysis of Variant (ANOVA) followed by Duncan (p<0,05). The result showed that there was significant effect from therapy on the quality of rats spermatozoa especially to capacitation and acrosome reaction. The treatment from treatment 2 group showed the best result in maintaining the quality of rats spermatozoa with 27.6± 3.04 values for capacitation and 6,80 ± 1,30 for acrosome reactions. Extract of pomegranate (Punicagranatum L.) standardized to 40% ellagic acid has a significant change in improve the quality of spermatozoa, namely an increase in the percentage of capacitation and acrosome reactions in white rats (Rattusnorvegicus) exposed to heat.