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All Journal Jurnal Veteriner Bioma Jurnal Biomedika Jurnal Kedokteran Hewan
Pudji Achmadi
Divisi Fisiologi, Departemen Anatomi Fisiologi Dan Farmakologi, Fakultas Kedokteran Hewan, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Hand-Powered Centrifuge Made from Cotton for Assessing Hematocrit Fathan Abdul Aziz; Pudji Achmadi; Mokhamad Fakhrul Ulum
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.657

Abstract

The hematocrit value test serves as a parameter to help reach an established diagnosis. A centrifuge is an equipment that is used to separate the components of blood from each other based on the properties of each constituent substance. This study aims to developing hand-powered centrifuge made from cotton material to assess the hematocrit of blood in pregnant and non-pregnant ewes. To make the Katunfuge, two circles were cut from a piece of cotton cloth that had been impregnated with batik wax. The knitting thread was inserted into the middle part of the cotton disc and each end rope was tied to the handler. The centrifugal speed resulted from Katunfuge was greater than 2,000 rpm, meaning that it is 99% effective for separating blood components in 15 minutes with 14 times of pulling with hands. The hematocrit values of pregnant ewes (33.0 ± 4.1) were lower than those of non-pregnant ewes (38.5 ± 3.4%). This has proven that Katunfuge is highly potential to be used to assess the hematocrit of other animals.
Pemberian Ekstrak Etanol Purwoceng pada Masa Pascaplasentasi Meningkatkan Kinerja Reproduksi Tikus Bunting (SUPPLEMENTATION ETHANOLIC EXTRACT OF PIMPINELLA ALPINA AT POSTPLASENTATION ON PREGNANT RAT IMPROVE REPRODUCTIVE PERFORMANCE) Aryani Sismin Satyaningtijas; Hera Maheshwari; Pudji Achmadi; Isdoni Bustaman; Bambang Kiranadi; Julianto .; Meta Levi Kurnia
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (95.862 KB)

Abstract

The study was carried out to observe the effect of ethanolic extract of estrogenic purwoceng given 13-21 days age of pregnancy on reproductive performance such as the weight of ovarium, uterus, placenta, andpups, correlation ratio of corpus luteum number, implantation site number, and litter size in rats. Thepregnant rats were divided in to two groups. One groups cosisted of rats treated with purwoceng (83.33 mg/kg body weight) and the other groups was used as control (no treatment). The ovary, uterus, placenta, andpups were collected and weighed on day 21 of pregnancy. The result showed that the rats given 83,33 mg/kg BW ethanol extract of purwoceng tended to increase the weight of ovarium, uterus, and pups but it didnot increase the weight of plasenta. Number of site of implantation, ratio of implantation site to numberof corpus luteum, and ratio of number of pups to implantation site were higher on rat given 83,33 mg/kgBW ethanol extract of purwoceng. Conclusion of this study is ethanol extract of Pimpinella alpina rootsgiven at postplasentation improve reproductive performance.
Pemberian Ekstrak Etanol Akar Purwoceng pada Tikus Bunting Selama Masa Organogenesis Memperpanjang Ukuran Tulang Anak Tikus (ADMINISTRATION ROOTS ETHANOL EXTRACT OF PAMPINELLA ALPINA TO PREGNANT RATS DURING ORGANOGENESIS EXTENDS THE BONE OF THEIR PUPS) Aryani Sismin Satyaningtijas; Pudji Achmadi; Hera Maheshwari; Isdoni Bustaman; Dirwan Rahman; Muhammad Zhaahir
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (88.472 KB)

Abstract

Pregnancy is an integral part of the circulatory system between pups and mother. Growth anddevelopment of pups is determined by intake from their mother. The purpose of this study was to measurethe growth of pup’s bones given ethanol extract purwoceng (Pampinella alpine) for 1 to 13 days of ratgestation. Eight pregnant rats were divided into two groups: control group and purwoceng group that wasgiven ethanol extract purwoceng at a dose of 25 mg/300 g bw. The born pups were observed for 70 days tomeasure the growth and development of head bone, back bone, front legs bone, and hind legs. The resultsshowed that the growth and development of all bones parameters of female pups were higher on purwocenggroup than control the bone while male pups did not difference between purwoceng and control group exceptback bone and hind leg bone. The conclusion of this study is that administration of purwoceng roots ethanolextract on pregnant rats during organogenesis phase can extends the bone length of their pups.
PENGARUH SUHU TERHADAP DIFERENSIAL LEUKOSIT SERTA KADAR MALONDIALDEHIDE (MDA) BURUNG PUYUH (Coturnix coturnix Japonica) Hera Maheshwari; Annissa Nuridfi Sasmita; Achmad Farajallah; Pudji Achmadi; Koekoeh Santoso
Bioma Vol 13 No 2 (2017): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (506.533 KB) | DOI: 10.21009/Bioma13(2).4

Abstract

The main problem in tropical poultry farming that often happens is a high mortality rate. Deaths occurrance mostly by heat stress, a condition which is caused by high temperature ( > 28 ° C). Prolonged heat stress was given to 24 quails grouped in four groups: A (39 ° C), B (41 ° C), C (43 ° C) and D (45 ° C). Provision of heat stress was done when the quails were 2 days old. Blood sampling was performed at the age of 6 weeks. Overall, there was an increase in the number of white blood cell, heterophile, eosinophil, monocyte, the ratio between heterophyll and lymphocyte and malondialdehyde (MDA) levels in all treatment groups. While the number of lymphocytes decreased in all treatment groups. Basophils had a significant increase in group C, but there was a decrease in group B and D decreased from control. This indicates that maintenance above temperature > 25 ° C can cause oxidative stress due to heat stress in quail.
KINERJA REPRODUKSI TIKUS BUNTING AKIBAT PEMBERIAN EKSTRAK ETANOL PURWOCEN Aryani Sismin Satyaningtijas; Hera Maheshwari; Pudji Achmadi; Wisnugroho Agung Pribadi; Sandra Hapsari; Divo Jondriatno; Isdoni Bustaman; Bambang Kiranadi
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (107.496 KB) | DOI: 10.21157/j.ked.hewan.v8i1.1253

Abstract

Pada penelitian ini, purwoceng diberikan pada tikus betina bunting umur 1-13 hari dengan tujuan mengetahui bobot badan, ovarium, dan uterus serta mempertahankan titik implantasi yang terbentuk sebelum masa implantasi. Penelitian ini menggunakan 2 kelompok yaitu kelompok tikus bunting kontrol dan tikus bunting yang mendapatkan purwoceng dengan dosis 25 mg/300 g bobot badan. Hasil penelitian menunjukkan bahwa kelompok tikus yang diberi purwoceng cenderung memiliki bobot ovarium dan uterus lebih berat dibandingkan kelompok kontrol. Purwoceng juga menyebabkan jumlah titik implantasi tikus hampir mendekati jumlah korpus luteum yang sudah terbentuk, dan ini menunjukkan bahwa keberhasilan implantasi lebih baik pada kelompok tikus yang diberi purwoceng.
KINERJA REPRODUKSI TIKUS BUNTING AKIBAT PEMBERIAN EKSTRAK ETANOL PURWOCEN Aryani Sismin Satyaningtijas; Hera Maheshwari; Pudji Achmadi; Wisnugroho Agung Pribadi; Sandra Hapsari; Divo Jondriatno; Isdoni Bustaman; Bambang Kiranadi
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.1253

Abstract

Pada penelitian ini, purwoceng diberikan pada tikus betina bunting umur 1-13 hari dengan tujuan mengetahui bobot badan, ovarium, dan uterus serta mempertahankan titik implantasi yang terbentuk sebelum masa implantasi. Penelitian ini menggunakan 2 kelompok yaitu kelompok tikus bunting kontrol dan tikus bunting yang mendapatkan purwoceng dengan dosis 25 mg/300 g bobot badan. Hasil penelitian menunjukkan bahwa kelompok tikus yang diberi purwoceng cenderung memiliki bobot ovarium dan uterus lebih berat dibandingkan kelompok kontrol. Purwoceng juga menyebabkan jumlah titik implantasi tikus hampir mendekati jumlah korpus luteum yang sudah terbentuk, dan ini menunjukkan bahwa keberhasilan implantasi lebih baik pada kelompok tikus yang diberi purwoceng.
Co-Authors A.S. Satyaningtijas ACHMAD FARAJALLAH Annissa Nuridfi Sasmita Bambang Kiranadi Bambang Kiranadi Dirwan Rahman Divo Jondriatno Fathan Abdul Aziz HERA MAHESHWARI Isdoni Bustaman Julianto . Koekoeh Santoso Meta Levi Kurnia Mokhamad Fakhrul Ulum Muhammad Zhaahir Sandra Hapsari Wisnugroho Agung Pribadi