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All Journal Jurnal Ilmu Pertanian Indonesia International Journal of Science and Engineering (IJSE) BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Penelitian Pascapanen Pertanian JURNAL TEKNIK INDUSTRI
Djumali Mangunwidjaja
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Electrical & Electronics Engineering Energy Engineering Immunology & microbiology Industrial & Manufacturing Engineering Veterinary

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Studi agrobiofisik kamandrah (croton tiglium L.) dan penentuan potensi awal kamandrah sebagai larvasida hayati pencegah penyakit demam berdarah dengue Dyah Iswantini; Rosihan Rosman; Upik Kesumawati; Djumali Mangunwidjaja; Min Rahminiwati; Adi Riyadhi
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 2 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1308.713 KB)

Abstract

The aim of the research is to obtain the ecological condition and propagation of kamandrah (Croton tiglium L.) and determination of its potency as biological larvacidal for preventing dengue haemorraghic fever. Agrobiophysic study indicated that Croton tiglium L. plant need full sunlight(> 70 %). low intensity of sunlight could decrease the ability of plant to produce seed. The plant could grow well on podzolic land (30-50 m upon sea surface). Seed of kamandrah from Ampah has growth percentage of 43.8 °/o, height of 27.5 em, stem diameter of 5 em, total leaves of 10, and total primary branches of 2. Phytochemical assay resulted that among other part of Kamandrah, Kamandrah seed has highest alkaloid content. Because alkaloid compound has high larvicidal activity, kamandrah seed has high potency as larvicide. Among all of extracts of part of plant, oil of Croton tiglium L. has the highest potency as biological larvacidal with 863.67 ppm of LC50 for 24 hours of treatment.
Potensi Jarak Pagar (Jatropha curcas) sebagai Larvasida Hayati Pencegah Penyakit Demam Berdarah Dengue Dyah Iswantini; Adi Riyadhi; Upik Kesumawati; Rosihan Rosman; Djumali Mangunwidjaja; Min Rahminiwati
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 1 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Recently, Indonesia has big problem caused by dengue haemorraghic fever with A. aegypti as vector. Potential medicine and vaccine for curing this disease have not been found. The effective method to prevent this disease is the use of A. aegypti larvicidal. The chemical larvicidal has disadvantage for evironmental aspect. To solve this problem, the biological larvicidal has a good chance to develop. Indonesia has megadiversity which can be developed as biological larvicidal. Crude aqueous extracts and ethanol extracts of Jatropha curcas (Jarak pagar) seed and oil of Jatropha curcas were evaluated for larvicidal potential against the Aedes aegypti mosquito. Among all extracts, Jatropha curcas oil possessed a highest activity against the 3th instar larvae of Aedes. LC50 values of Jatropha curcas oil was 1507 ppm for 24 h and 866 ppm for 48 h. It was suggested that the Croton tiglium oil and Jatropha curcas oil possess larvicidal properties that could be developed and used as biological larvicidal. 
Optimasi Kondisi Isomerisasi Eugenol Dari Minyak Daun Cengkeh Menggunakan Metode Permukaan Respon Edy Mulyono; Ani Suryani; Djumali Mangunwidjaja; Dwi Setyaningsih
Jurnal Penelitian Pascapanen Pertanian Vol 7, No 2 (2010): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v7n2.2010.86-93

Abstract

Eugenol merupakan komponen utama minyak cengkeh yang kandungannya dapat mencapai 70-95 % tergantung dari bahan baku yang digunakan (bunga, tangkai, dan daun), Dengan kandungan eugenol yang tinggi, minyak cengkeh merupakan sumber bahan baku isoeugenol yang potensial. Tujuan dari penelitian ini adalah untuk mengetahui kondisi proses optimum proses isomerisasi eugenol dari minyak daun cengkeh. Metode yang digunakan dalam penelitian ini adalah rancangan percobaan dan analisis deskriptif. Hasil analisis ragam pada tahap isomerisasi isoeugenol rnenunjukkan konsentrasi katalis rhodium trichloride trihydrate dengan waktu pemanasan berpengaruh nyata terhadap kadar produk (isoeugenol, cis-isoeugenol dan trans-isoeugenol) dan jumlah bahan yang menguap, Hasil optimasi dengan menggunakan response surface method menunjukkan taraf faktor perlakuan berupa 1.) konsentrasi katalis 0,16% dan 2,) waktu pemanasan selama 10 menit merupakan perlakuan yang memberikan respon yang optimal dengan nilai desirability (D) 0,691, Kombinasi perlakuan ini memberikan nilai respon pada kadar isoeugenol 86,25%, kadar cis-isoeugenol 18,73%, kadar trans-isoeugenol 67,54% dan jumlah bahan menguap 12,28%, Hasil analisis spektroskopi FTIR dan HNMR menunjukkan pita serapan adalah identik antara isoeugenol hasil isomerisasi dengan standar. Hasil analisis FTIR dari sampel isoeugenol menunjukkan pita serapan gugus OH pada angka gelombang 3498,87 cm-1 std. 3496,94 cm-1), pita serapan di angka gelombang 2846,93 cm-1 (std. 2846,93 cm-1) menunjukkan adanya gugus OCH3 gugus C-C aril (cincin aromatis) pada angka gelombang 1598,99 cm-1 (std. 1598,99 cm-1) dan gugus CH, pada angka gelombang 2935,66 cm-1 (std, 2935,66 cm-1). Sedangkan hasil analisis dengan HNMR menunjukkan posisi pergeseran kimia (a) gugus metil (-CH3) sebagai identitas senyawa isoeugenol, yaitu sebesar 1,812 ppm (std. 1,814 ppm) berbentuk doublet untuk 3H dari -CH3. Hasil identifikasi juga menunjukkan bahwa senyawa isoeugenol yang diperoleh lebih dorninan dalam bentuk trans isoeugenol. Spektrum FTIR menunjukkan bahwa pita serapan trans-isoeugenol (= CH) yang berlokasi di angka gelombang 962,4 cm-1 sedangkan cis-isoeugenol terletak di angka gelombang 792,7 cm-1. Sedangkan hasil spektrum HNMR untuk puncak H pada pergeseran kimia a=6,1 dan 3=5,95 ppm, Optimization of Eugenol Extraction from Clove Leaf Using Response Surface MethodologyEugenol is the main component of clove oil. Its content can reach 70-95% depending on the raw materials (flower, stem, and leaves) used for distillation, With a such high content of eugenol, clove oil is a potential source for synthesis of isoeugenol through isomerization process" The purpose of this study was to determine the optimum process condition of eugenol isomerization process, The method used in this research is the experimental design and descriptive analysis, Results of analysis of variance showed that the concentration of catalyst rhodium trichloride trihydrate and beating time significantly affected the content of product (isoeugenol, cis-isoeugenol, trans-isoeugenol) and the amount of material evaporated, The result of optimization by using response surface method showed that level of treatment factors i.e. I) Catalyst concentration of 0,16%, and 2) Heating time for [0 minutes, were treatments that provides the most optimal response to the desirability value (D) 0,691. These level of treatment factors gave the response value at isoeugenol content of 86.25%, cis-isoeugenol content of 18.73%, trans-isoeugenol content of 67,54%, and the amount of evaporated material 12,28%, The FTIR spectroscopic and HNMR, analysis showed characteristic absorption bands are identical between the isomerization and isoeugenol standards, The result of FTIR analysis of isoeugenol samples showed absorption band of OH group at 3498,87 cm-1 (std. 3496,94 cm-1), absorption band at 2846,93 cm-1 (std. 2846.93 cm-1) which indicates the group OCH 3 C-C aryl group (aromatic ring) at 1598,99 cm-1 (std. 1598,99 cm-1) and CH3 group at 2935,66 cm-1 (std. 2935,66 cm-1) 'While the results of the analysis with HNMR indicate the position of chemical shift (3) of methyl group (-CH 3) as the identity of isoeugenol compound which is 1,812 ppm (std. 1,814 ppm) in the form of doublets for the 3H from -CH 3, The identification results also showed that the isoeugenol obtained was more dominant in the form of trans isoeugenol. FTIR spectra showed that the absorption band of trans-isoeugenol (=CH) was located at 962.4 cm-1 where as the cis-isoeugenol was located at 792,7 cm-1, While the results of HNMR spectrum for peak of H was located at ppm 3=6.1. and a=5,95,
PRODUKSI PEKTIN BERMETOKSIL RENDAH DARI KULIT JERUK NIPIS (Citrus aurantifolia Swingle) SECARA SPONTAN MENGGUNAKAN PELARUT AMONIUM OKSALAT DAN ASAM Sri Usmiati; Djumali Mangunwidjaja; Erliza Noor; Nur Richana; Endang Prangdimurti
Jurnal Penelitian Pascapanen Pertanian Vol 13, No 3 (2016): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v13n3.2016.125-135

Abstract

Pengembangan teknologi ekstraksi pektin bermetoksil rendah terus dieksplorasi karena tanaman sumber yang relatif terbatas. Tujuan penelitian adalah untuk memperoleh pektin bermetoksil rendah langsung dari ekstraksi kulit jeruk nipis (Citrus aurantifolia Swingle). Penelitian didahului oleh dua tahap penelitian pendahuluan menggunakan rancangan acak lengkap yang masing-masing bertujuan memperoleh suhu ekstraksi (tahap pertama) dan lama waktu ekstraksi (tahap kedua) terbaik untuk digunakan sebagai kondisi proses ekstraksi pada penelitian utama. Desain penelitian utama menggunakan rancangan acak lengkap pola faktorial dengan tiga ulangan. Faktor pertama adalah tipe pelarut (P): P1 (amonium oksalat+asam oksalat, asam sitrat) dan P2 (amonium oksalat+asam oksalat, asam klorida), dan faktor kedua pH (K): K1 (pH 1) dan K2 (pH 2), serta sebagai pembanding adalah menggunakan pelarut P0 [asam sitrat, asam klorida; pH 2,0]. Ekstraksi suksesif dua tahap menggunakan suhu 100oC dilakukan selama 45 menit dengan pelarut amonium oksalat+asam oksalat, dilanjutkan 10 menit menggunakan pelarut asam sitrat atau asam klorida. Parameter pengukuran meliputi rendemen, derajat esterifikasi (DE), kadar asam uronat, kadar metoksil, kadar air, kadar abu, serta berat ekivalen. Dari hasil penelitian diketahui bahwa interaksi tipe pelarut dan pH pada perlakuan P2K2 (amonium oksalat+asam oksalat, asam klorida) dapat langsung menghasilkan pektin bermetoksil rendah dari kulit jeruk nipis dengan nilai DE 45,77%, kadar metoksil 1,53% dan kadar abu 4,77%. Pelarut amonium oksalat+asam oksalat (t1) dan asam klorida (t2) (P2) pada kedua tingkat pH menghasilkan rendemen pektin 10,0%, kadar AUA 19,21%, dan nilai BE 1878,82. Kadar air pektin sebesar 7,91% dihasilkan dari tingkat pH 2 (K2) pada kedua tipe pelarut. Untuk memperoleh pektin bermetoksil rendah dari kulit jeruk nipis direkomendasikan menggunakan pelarut P2K2 (amonium oksalat+asam oksalat, asam klorida; pH 2) pada suhu ekstraksi 100oC.English Version AbstractSpontaneously production of low methoxyl pectin from peel of Citrus aurantifolia Swingle using solvent of ammonium oxalate and acidThe development of extraction technology of low methoxyl pectin/LMP continue to be explored caused by limited the plant source. The research objective was to obtain low methoxyl pectin directly from the pectin extraction of lime peel (Citrus aurantifolia Swingle). The study was preceeded by a twostage preliminary researches using a completely randomized design, each of which was to obtain the best temperature (first stage) and duration of extraction (second stage) to be used as a condition of extraction process in the primary research. The primary research used factorial completely randomized design with three replications. The first factor was solvent type (P): P1 (ammonium oxalate+oxalic acid), citric acid) and P2 (ammonium oxalate+oxalic acid, hydrochloric acid), and the second factor was pH (K): K1 (pH 1) and K2 (pH 2), as well as control was solvent P0 [citric acid (t1), hydrochloric acid (t2); pH 2.0]. Successive two-stage extraction on 100°C was done for 45 minutes using ammonium oxalate+oxalic acid followed by 10 minutes using citric acid or hydrochloric acid. Measurement parameters included yield, degree of esterification (DE), anhidrouronic acid/AUA levels, methoxyl content, moisture content, ash content, and equivalent weight. From the results of research, the interaction between solvent tipe and acidity level of P2K2 (ammonium oxalate+oxalic acid, hydrochloric acid; pH 2) could directly produce LMP from extraction of lime peel characterized by DE of 45.77%, methoxyl content of 1.53% and ash content of 4.77%. Effect of solvent of ammonium oxalate+oxalic acid, hydrochloric acid (P2) at both pH levels resulted pectin yield of 10.0%, AUA of 19.21%, and equivalent weight of 1878.82. The pectin moisture of 7.91% was produced from acidity of pH 2 (K2) on both type of solvent. To obtain LMP from peel of Citrus aurantifolia Swingle it was recommended to use the extraction solvent of P2K2 (ammonium oxalate+oxalic acid, hydrochloric acid; pH 2) on temperature of 100oC.
Isolation and Fermentation of Lactobacillus plantarum JR64 as an Omega 6 Probiotic Producer Lanjar Sumarno; Djumali Mangunwidjaja; Anas M. Fauzi; Khaswar Syamsu; Nastiti Siswi Indrasti; Bambang Prasetya
International Journal of Science and Engineering Vol 2, No 2 (2011)
Publisher : Chemical Engineering Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (596.963 KB) | DOI: 10.12777/ijse.2.2.42-47

Abstract

Application of lactic acid probiotic bacteria in health food diversification currently is progressing rapidly.  It is encouraged the study of searching the potential strains from local resources (Ponorogo Residence) namely noni fruits (badeg pace) and noni wine.  Aims of this study were to perform the isolation, identification, and production of  probiotic Lactobacillus sp. JR64 fermentation process as a producer of Omega-6 (ω-6) lowering cholesterol and design of probiotic creamy product.   Beginning stages of research was strains isolating and in-vitro testing, the best result were used in  molecular  identification technology development for the production of metabolites through the manipulation of environmental variation  of glucose 20 g / l, 30 g / l and 40 g / l that influenced the substrate concentration of linoleic acid productivity. The result of new isolates isolation showed that isolates that obtained from noni wine, Lactobacillus plantarum JR64, was potential as probiotic condidate.   The effieciency of fermentation  substrates using  Yx/s and Yp /s  in the exponential phase was the highest value for the fermentation of 24 hours of  Yx/ s; 17.03% and Y p/s; 74.72%, while the results of design and the best formulation for viability cells of lactobacillus  plantarum probiotics JR64 was composed of 15 g and 50g butter 15 g icing sugar as well as during storage of the refrigerant temperature was 8.92 x 108 CFU / ml.  [Key Words : Omega-6,  Lactobacillus plantarum JR64, fermentation]
ANTIBREAST CANCER ACTIVITY OF NANOPROPOLIS INDONESIA ON INDUCED MAMMARY GLAND TUMOR BY DMBA IN VIRGIN SPRAGUE-DAWLEY RATS Akhmad Endang Zainal Hasan; Djumali Mangunwidjaja; Titi Candra Sunarti; Ono Suparno; Agus Setiyono
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 23 No. 1 (2016)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (613.285 KB) | DOI: 10.11598/btb.2016.23.1.473

Abstract

The objective of this study was to determine the effect of nanopropolis to cure cancer induced on rat mammary tumor using 7,12-dimethylbenz(a)anthracene (DMBA). After the first tumors appearance, twenty eight rats were divided into seven groups. Group 1, 2 and 3 served as recipient of nanopropolis dosages 8, 32 and 56 µg/mL treatments; Group 4 served asrecipient of propolis dosage of 233 µg/mL treatment; Group 5 served asrecipient of doxorubicin treatment; Group 6 served as recipient of DMBA treatment and Group 7 as normal group (control). The effect of nanopropolis dosage of 32 µg/mL and propolis dosage of 233 µg/mL were similar in reducing tumor size, healing the wounds caused by the tumor and eliminating cancer cells. It turns out that there is a relationship between particle size absorbent materials. The study suggested that nanopropolis with small concentration was very effective to treatrat mammary gland tumors and breast cancers.Keywords: breast cancer, nanopropolis, propolis, Sprague-Dawley rat
PERANCANGAN MODEL PENERIMAAN DAN EVALUASI PESANAN PADA INDUSTRI KEMASAN KARTON YANG BERBASISKAN MAKE TO ORDER Nora Azmi; Irawadi Jamaran; Yandra Arkeman; Djumali Mangunwidjaja
JURNAL TEKNIK INDUSTRI Vol. 2 No. 1 (2012): Volume 2 No 1 Maret 2012
Publisher : Jurusan Teknik Industri, Fakultas Teknologi Indusri Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (765.928 KB) | DOI: 10.25105/jti.v2i1.7011

Abstract

Nowadays, carton boxes are very popularly used, mostly in food industries, pharmacy and other consumer products. Most carton box industries work base on customer order (Make-to-order company), because most of carton box order are prepared for particular customer. Carton box are not only used for containing and protecting the product, but also functioned to inform the product to the customers. Make-to-order company has a high level of uncertainty such as the type and design of the product, the step of production, production time, cost, equipments and machine. This could create further problems; the overdue of order and completing process, and the acceptability of the product. This research is intended to design an order processing model for the carton box industry to accelerate order receipt process and evaluate the feasibility of the order. This paper focused on two models, those are the order entry model and order evaluation model. In order entry model, product code is given, and calculation model for needed raw material is made by using mathematical formulation. In order evaluation model, expert system and decision tree are used for analyzing the process capability and selecting machinery as well as designing the steps of production.
PENJADWALAN PESANAN MENGGUNAKAN ALGORITMA GENETIKA UNTUK TIPE PRODUKSI HYBRID AND FLEXIBLE FLOWSHOP PADA INDUSTRI KEMASAN KARTON Nora Azmi; Irawadi Jamaran; Yandra Arkeman; Djumali Mangunwidjaja
JURNAL TEKNIK INDUSTRI Vol. 2 No. 2 (2012): Volume 2 No 2 Juli 2012
Publisher : Jurusan Teknik Industri, Fakultas Teknologi Indusri Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (563.61 KB) | DOI: 10.25105/jti.v2i2.7028

Abstract

This research was intended to produce an order (job) scheduling model at Carton Packaging Industries (CPI) that is useful for giving information about the time delivery to customers. The proposed model is quite complicated because of the characteristic of Make to Order (MTO) varies production process greatly between each order. The job’s schedule for CPI is prepared for production process that consists of 5 stages where in each stage uses different type of machinery. Not all jobs can be processed by all machines at a given production stage. Every job flow through 5 stage in the same order, but not all stages have to visited by all jobs. Stages may be skipped for a particular job. This condition makes CPI is classified as Hybrid and flexible flowshop for machine eligibility (HFFME). HFFME is complicated and is difficult to calculate by using conventional heuristic model. This research used genetic algorithm for solving the complex problem of HFFME and the resulting model called the Genetic Algorithm for hybrid and flexible flowshop with machine eligibility (GA-HFFME). This model is developed to minimized makespan, the objective of scheduling. The experiment was conducted towards 11 orders and it was found that the GA-HFFME is effective to solve that problem.
Co-Authors Adi Riyadhi Agus Setiyono Akhmad Endang Zainal Hasan Anas M. Fauzi Ani Suryani Bambang Prasetya Dwi Setyaningsih Dyah Iswantini Edy Mulyono Eka Ruriani Endang Prangdimurti Erliza Noor Irawadi Jamaran Khaswar Syamsu Lanjar Sumarno Min Rahminiwati Nastiti Siswi Indrasti Nora Azmi Nur Richana Ono Suparno dan Eko Wahyudi Rosihan Rosman Sri Usmiati Titi Candra Sunarti dan Michael (E-Jurnal Agro-Industri Indonesia) Upik Kesumawati Hadi Yandra Arkeman