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All Journal Buletin Peternakan Jurnal Sains Teknologi dan Lingkungan (JSTL) Jurnal Pengabdian Magister Pendidikan IPA JITPI : Jurnal Ilmu Dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Science and Technology)
Adji Santoso Dradjat
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Immunology & microbiology Public Health Veterinary Other

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Motilitas Spermatozoa Ayam (Galus varius) Pada Penyimpanan Dingin Dengan Pengencer Tris, Cytrate, Kuning Telur, Filtrat Jambu Biji (Psidium guajava) Dan Buah Tin (Fikus karika rob) Adji Santoso Dradjat; Lukman H.Y.; Sumadiasya I.W.L.
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 4 No 1 (2018): Jurnal Ilmu dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Scie
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.618 KB) | DOI: 10.29303/jitpi.v3i1.38

Abstract

The objectives of this study were to evaluate rooster semen preserved using Tris Citrate Egg Yolk-Citrate (TEYC) extender with the addition of Tin fruit and guava filtrate. Semen was collected by using plastic collector placed on cloaca, by using hen as a teaser when rooster mated hen consequently semen would remain in the plastic collector. Fresh semen was evaluated by evaluating volume, smell, color and spermatozoa concentration. Then semen was split into 7 fractions as treatment 1, 2 and 3 were extended 10 times using TEYC with 20%, 25% and 30% egg yolk. Then treatment 4 and 5 were extended using TEYC contain 20% egg yolk with 5 % and 10% guava filtrate. Treatments 6 and 7, were extended using TECY contain 20% egg yolk with Tin fruit filtrate of 5 and 10%. Then preserved and progressive motility was evaluated at 0, 12, 24 and 36 hours following chilling under 4°C. Results showed that ejaculate volume was 0.38 ± 0.14 ml, specific smell, milky white color with concentrations of 511.00 ± 90.07 x 107 sperms/ml. The results of progressive motility evaluation of 0, 12, 24 and 36 hours following chilling treatments 1 to 7 were not significantly different (P> 0,05). Percentage motility of chilling 0 hours and 36 hours of treatments 1 to 7, were from 86.50 ±4.72% to 48.83 ±5.15%, from 83.83 ±7.41% to 47.83 ±7.44%, from 88.67 ±1.86% to 49.33 ±9.35%, from 83.00 ±4.69 % to 43.50 ±7.77%, from 82.67 ± 6.06%, to 46.33 ±5.75%, from 83.83 ±7.41% to 47.83 ±7.44%, from 88.67 ±1.86% to 49.33 ±9.35% respectively and the average decreasing progressive motility up to 36 hours was 37.74 ± 1.64 %. Finally, it can be concluded that addition egg yolk, guava, and Tin fruit filtrate do not influence the progressive motility of local rooster spermatozoa.
Efektifitas Kinerja Pentoksifilin Terhadap Kualitas dan Integritas Membran Plasma Utuh pada Sperma Sapi Bali Hasil Pemisahan dengan Menggunakan Albumin Rodiah Rodiah; Enny Yuliani; Adji Santoso Dradjat; Chairussyuhur Arman
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 1 No 1 (2015): Jurnal Ilmu dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Scie
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.932 KB) | DOI: 10.29303/jitpi.v1i1.12

Abstract

The purpose of this research was to determine the effect of Pentoxyfilline in maintaining the quality (motility, viability, morphology and plasma membrane intact) of Bali bull spermatozoa separated with albumine. A Completely Random Block Design was used in this study with two main factors (upper layer and bottom layer), diluents with four doses of pentoxyfilline (0, 15, 20 and 25 μM) with six replications. Evaluation of spermatozoa was performed by characterizing motility, viability, morphology, plasma membrane intact. Result of the study showed that the effect of four doses pentoxyfilline on the quality of Bali bull spermatozoa was non significant (p>0,05). However, the addition of 25μM pentoxyfilline was found to give the best effect compared to 15 μM, 20 μM and control. Result of the study also indicated that spermatozoa separated in the upper layer was signifiqantly higher (p<0,01) than that of separated in the bottom layer with the average motility, viability, morfology and plasma membrane intact was 66,67,80,50, 6,71 and 65,61% respectively.
Identifikasi Komponen, Faktor dan Resiko Relatif yang Berpengaruh pada Hasil Inseminasi Buatan pada Sapi Bali Adji Santoso Dradjat; Rodiah Rodiah; Enny Yuliani; Chairussyuhur Arman
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 2 No 1 (2016): Jurnal Ilmu dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Scie
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.266 KB) | DOI: 10.29303/jitpi.v2i1.26

Abstract

The aims of the research were to identify components, factors and relative risk (Odd ratio) which may influencethe artificial insemination results. The study were performed in two villages those were Kuranji and Tanah Beak,by identification of components and factors which may contribute to successful artificial insemination results,then the flow chart of successful and obstacle were designed. Based on the flow chart survey was performed to thefarmers and inseminators. Parameters evaluated were factors may influence of the result of insemination such as;management, oestrus detection by farmers and insemination by inseminators. The data was tabulated and analisedusing epidemiology analysis of odd ratios (OR). The results showed that insemination results of Kuranji villagedo not have the risk to be not pregnant as the odd ratio was less than 1 (OR <1,0), with the range of 0,10 to 0,20.While artificial insemination in Tanah Beak village showed risk of cows to be infertile or non pregnant relativelyhigher with OR higher than 1 (OR> 1.0), with the range of 1,5 to 2,0. The results showed that the risk to beunsuccessful to produce pregnancy were caused by oestrus detection, feeding management, skill inseminator andexperience of farmers. Finally it can be concluded that oestrus detection by farmers, feeding management,insemination by inseminator and experience of farmers may contribut to the high risk fail to produce pregnancyusing artificial insemination in Tanah beak village.
Analisis Tingkat Pengetahuan, Sikap dan Perilaku Peternak Unggas Terhadap Upaya Pencegahan Penyakit Flu Burung Rusman Effendi; Adji Santoso Dradjat; Made Sriasih
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 2 No 1 (2016): Jurnal Ilmu dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Scie
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.527 KB) | DOI: 10.29303/jitpi.v2i1.21

Abstract

The aim of this research was to describe and to correlate knowledge, attitude and practice (KAP) of thepoultry breeders in preventing Avian Influenza (AI). This research used face-to-face interview, cross sectionaldesign study with a questionnaire for 100 respondentsand data were analyzed with Rho Spearman. The resultshowed that 82% of respondents washed their hands with soap after handling poultry but only 5% ofrespondents used mask when contacting with poultry. Chickens were kept by respondents most. The cagelocation near to the house accounted for 71%, most of respondents cleaned the cage routinely but there were11% of respondents who did not keep their poultry into the cage. All respondents revealed that the poultrywere not vaccinated periodically. It was about 37.3% of respondents burned dead poultry, and 55.2% and 22.4% buried and throw away to the river respectively. They disagreed with stamping out accounted for 48% andonly 16% of respondents agreed to sell their unhealthy poultry. No respondents informed to the authority ofsub-village when suddent death of the chickens was found. Respondent’s knowledge, attitude and practicewere suffient in preventing Avian Influenza and there was significant correlation between knowledge, attitudeand practice (P<0,05).
Komparasi Bioetri Semen dan Morfometri Spermatozoa Kambing Kacang, Ettawa dan Boer Lalu Ahmad Zaenuri; Rodiah Rodiah; Adji Santoso Dradjat; I Wayan Lanus Sumadiasa
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 7 No 1 (2021): Jurnal Ilmu dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Scie
Publisher : Faculty of Animal Husbandry, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jitpi.v7i1.85

Abstract

Penelitian ini bertujuan untuk mengetahui perbedaan biometri dan morfometri spermatozoa kambing Kacang, Peranakan Ettawa dan Boer. Penampungan semen dilakukan menggunakan vagina buatan. Satu bagian semen segar diencerkan dengan sepuluh bagian NaCl fisilogis (v/v). Fiksasi slide dengan cara mencampur satu tetes semen encer dengan satu tetes larutan Eosin-nigrosin, homogenkan dan keringkan diatas hot plate suhu 40°C selama beberapa menit. Biometri dan Morfometri spermatozoa diukur dengan mikroskop phase contras (CarlZeis, Jerman) pembesaran 400 kali. Semen dari setiap kambing dibuatkan satu slide dan setiap slide diambil 10 sampel spermatozoa hidup, sehingga sampel spermatozo untuk setiap jenis kambing adalah 50 spermatozoa. Signifikansi antar variabel diuji menggunakan T-test dengan pengujian independent samples test dengan tingkat signifikansi 0.05. Hasil penelitiann menunjukkan, tidak ada perbedaan nyata variabel biometri ketiga jenis kambing tersebut, kecuali konsentrasi, motilitas massa dan motilitas individu spermatozoa kambing PE secara signifikan lebih rendah dibanding kambing Boer dan Kacang. Panjang kepala spermatozoa kambing kacang, Ettawah dan Boer, tidak berbeda nyata. Sebaliknya, lebar kepala spermatozoa kambing Boer cendrung lebih lebar tetapi tidak berbeda nyata dibanding kambing Kacang dan kambing PE. Sedangkan panjang ekor spermatozoa kambing Boer secara signifikan lebih pendek dibanding kambing Kacang dan kambing PE. Disimpulkan, Morfomerti spermatozoa kambing Kacang PE dan Boer tidak berbeda nyata. Disarankan supaya hasilnya lebih akurat, pengukuran morfomerti spermatozoa hendaknya dilakukan pada spermatozoa dengan kromosom yang sama yaitu X atau Y saja
Evaluasi Penyimpanan Spermatozoa Ayam Pada Suhu 5⁰C, 26⁰C Dengan Pengencer Infuse NaCl, Glukosa 5% dan 10% Asnawi Asnawi; Maskur Maskur; Adji Santoso Dradjat
JURNAL SAINS TEKNOLOGI & LINGKUNGAN 2021: Special Issue, Oktober 2021
Publisher : LPPM Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jstl.v0i0.242

Abstract

The purpose of this study were to compare the quality of spermatozoa stored at 26⁰C, 5⁰C using diluents of NaCl, 10% glucose and 5% glucose. The spermatozoa of a rooster was collected and divided into 6 parts, each 2 tubes diluted in a ratio of 1:1 using NaCl, Glucose5% and Glucose 10%, then each 3 tubes with different diluents were stored at 26⁰C and 5⁰C. Observations of motility, viability and abnormalities of spermatozoa were carried out half an hour, 1 hour after dilution, followed every 2 hours until the ninth hours. The results showed that spermatozoa stored for 9 hours at a temperature of 26⁰C with a physiological diluent of NaCl, 10% Glucose and 5% Glucose each were different (P, < 0.05) with motility 50 ± 0.0%, 42 ± 10.95. % and 34±8.94%, respectively. At storage temperature of 5⁰C for 9 hours, physiological NaCl, 10% glucose and 5% glucose were significantly different (P<0.05) with motility 58.00±10.95%, 46.00±8.94% and 38.00±, respectively. 10.95% in a row. The viability of spermatozoa at 26⁰C storage with 5% glucose diluent was better than 10% glucose and physiological NaCl (P<0.05), 58.93±1.27%, 42.93±1.48% and 33.43±1.27% , while the physiological NaCl diluent and 10% glucose were not significantly different (P>0.05). At 5⁰C storage the viability of spermatozoa in the three diluents was not significantly different, with values of Glucose 10%, Glucose 5% and physiological NaCl 52.57±5.15%, 52.21±5.02% and 48.14±8.09%, respectively. Spermatozoa abnormalities at storage temperature 26⁰C and 5⁰C for 9 hours using physiological NaCl diluent, 5% glucose and 10% glucose, were not significantly different and varied between 5 to 10%. Finally, it can be concluded that at room temperature storage less than 4 hours the quality of spermatozoa was better with 5% glucose diluent, while for cold storage beyond 4 hours the quality of spermatozoa with NaCl diluent was higher
Effect of Post-Partum on The Oestrus Synchronisation with Progesterone and Oestradiol of Bali Cattle Adji Santoso Dradjat
Buletin Peternakan Vol 25, No 2 (2001): Buletin Peternakan Vol. 25 (2) Mei 2001
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v25i2.1429

Abstract

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Relation of Antler Stages and Reproductive Activity of Bawean Deer (Axis kuhlii) Stags Adji Santoso Dradjat
Buletin Peternakan Vol 25, No 4 (2001): Buletin Peternakan Vol. 25 (4) November 2001
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v25i4.1441

Abstract

Artikel dalam bentuk PDF
Sosialisasi Keuntungan Inseminasi Buatan Pada Sapi Bali di Kelompok Peternak Sapi desa Sapit Kecamatan Suela Kabupaten Lombok Timur Lalu Zaenuri; Rodiah; Adji Santoso Dradjat; I Wayan Lanus Sumadiasa; Lukman HY; Enny Yuliani
Jurnal Pengabdian Magister Pendidikan IPA Vol 6 No 4 (2023): Oktober-Desember 2023
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v6i4.5515

Abstract

Beternak sapi merupakan kegiatan keseharian petani/peternak di Provinsi Nusa Tenggara Barat (NTB), sehingga tidak mengherankan jika populasi sapi cendrung meningkat dari tahun ke tahun. Persentase peningkatan populasi sapi tercatat sebesar 23,23% yaitu dari 1.013.793 ekor pada tahun 2014 menjadi 1.320.551 ekor pada tahun 2021. Demikian juga halnya dengan pelaksanaan program Inseminasi Buatan (IB) pada sapi yang sudah diterima secara luas oleh peternak hampir di seluruh wilayah Nusa Tenggara Barat. Tetapi, kegiatan IB di desa Sapit sempat terhenti beberapa waktu akibat merebaknya penyakit mulut dan kuku (PMK). Untuk mengaktifkan kembali kegiatan IB, telah dilaksanakan sosialisasi mengenai “keuntungan Inseminasi Buatan Pada Sapi Bali”. Kegiatan ini telah terlaksana sesuai rencana. Evaluasi yang dilakukan setelah selesai sosialisasi diketahui bahwa, pengetahuan mengenai IB meningkat dari 60% sebelum sosialisasi menjadi 100% setelah sosialisasi. Demikian juga Keinginan untuk melakukan IB meningkat dari 45% menjadi 90%, sisanya 10% menyatakan tidak berminat. Keinginan untuk memiliki anak sapi hasil IB meningkat dari 55% menjadi 100%. Walaupun demikian 45% peserta mengatakan keberhasilan IB di wilayahnya masih rendah yaitu sekitar 45%. Penyebab relatif rendahnya keberhasilan IB menurut seluruh peserta salah satunya adalah karena ketersediaan pakan yang fluktuatif. Disimpulkan, program pengabdian kepada masyarakat telah dilaksanakan sesuai rencana. Materi yang disampaikan mendapat sambutan positip dari seluruh peserta karena sangat sesuai dengan kebutuhan peternak dilokasi pengabdian, terbukti dari minat peserta untuk melaksanakan program IB meningkat 100% dari 45% sebelum sosialisasi menjadi 90% setelah sosialisasi. Disarankan, sosialisasi mengenai IB harus lebih diinsifkan melalui pertemuan informal inseminator dan paramedis dengan peternak serta memberikan pelatihan pengawetan pakan ternak pada musim-musim tertentu ketika produksi hijauan pakan ternak melimpah.
Co-Authors Asnawi Asnawi Chairussyuhur Arman Enny Yuliani I Wayan Lanus Sumadiasa Lalu Ahmad Zaenuri Lalu Zaenuri Lukman H.Y. Lukman HY Made Sriasih Maskur Maskur Rodiah Rodiah Rodiah Rusman Effendi Sumadiasya I.W.L.