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All Journal Jurnal Veteriner Jurnal Ilmu Ternak Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Jurnal Ilmu dan Teknologi Peternakan Tropis Livestock and Animal Research JITPI : Jurnal Ilmu Dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Science and Technology)

Edy Kurnianto

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Author-ID : 2443734

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary Other

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Articles

Polimorfisme Protein Darah Ayam Kedu Jengger Merah dan Jengger Hitam di Satuan Kerja Non Ruminansia Temanggung Bagus Praditya Setyo Nugroho; Sutopo Sutopo; Edy Kurnianto
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 2 No 1 (2016): Jurnal Ilmu dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Scie
Publisher : Faculty of Animal Husbandry, University of Mataram

The objectives of this study were to determine the blood protein diversity first generation of Kedu chickens (G0)at Satuan Kerja Non Ruminansia Maron, Temanggung. The materials used in this study were each of 14 red andblack comb Kedu chickens. The methods of this study were observational and electrophoresis PAGE(Polyacrylamid Gel Electrophoresis). The observed parameters were loci of pre albumin (Palb), albumin (Alb),ceruloplasmin (Cp), transferrin (Tf), post transferrin (Ptf) and amylase-I (Amy-I). Gene structures were observedto characterize genetic diversity. Characterization of genetic diversity based on individual heterozygosity, meanheterozygosity and chi-square values for testing Hardy Weinberg equilibrium for each observed loci. The resultsshowed that red comb Kedu chickens had the highest gene frequencies of pre albumin, ceruloplasmin, transferrin,post transferrin and amylase-I those were 0,714; 0,714; 0,643; 0,643 and 0,536 respectively. The highestindividual heterozygosity of red comb Kedu chickens found in amylase-I (0,497) and also post transferrin (0,500),and amylase-I (0,500) for black comb Kedu chickens. The highest mean heterozygosity was found in black combKedu chickens (0,458). The chi-square calculation showed that the whole loci were at Hardy Weinbergequilibrium except pre albumin at red comb Kedu chickens.

Evaluasi Keunggulan Genetik Sapi Peranakan Ongole Betina Dengan Dua Metode Yang Berbeda Di Satker Sumberrejo-Kendal Arista Seftiana; Sutopo Sutopo; Edy Kurnianto
Jurnal Ilmu dan Teknologi Peternakan Indonesia (JITPI), Indonesian Journal of Animal Science and Technology Vol 5 No 1 (2019): Jurnal Ilmu dan Teknologi Peternakan Indonesia (Indonesian Journal of Animal Scie
Publisher : Faculty of Animal Husbandry, University of Mataram

Tujuan dari penelitian ini adalah untuk menentukan keunggulan sapi Ongole Grade (PO) di Satker-Sumberrejo, Kendal menggunakan dua metode yang berbeda, yaitu MPPA dan EBV. Bahan yang digunakan dalam penelitian ini adalah 48 ekor sapi.Metode yang digunakan dalam penelitian ini adalah observasi menggunakan rekaman untuk mendapatkan catatan silsilah sapi, kemudian digunakan dalam perhitungan.Korelasi Spearman digunakan untuk membandingkan dua metode seleksi yaitu MPPA dan EBV. Hasil penelitian ini menunjukkan rata-rata berat lahir adalah 29,35 ± 2,13 kg sedangkan berat sapih 112,58 ± 24,17 kg. Berat badan standar untuk berat lahir adalah 30,91 ± 1,97 kg, sedangkan untuk berat menyapih adalah 110,10 ± 11,20 kg. Uji korelasi Spearman menunjukkan tidak ada perbedaan yang signifikan antara MPPA dan EBV.Dapat disimpulkan bahwa MPPA dan EBV dapat digunakan dalam pemilihan sapi.

Polimorfisme Protein Serum Darah Induk Sapi Beranak Kembar dan Tunggal pada Sapi Peranakan Ongole dan Keturunan Simental (BLOOD SERUM PROTEIN POLYMORPHISM OF THE COW DELIVERED TWIN OR SINGLE CALVES IN ONGOLE GRADE AND SIMENTAL CROSSBRED) Tri Yuwono; Irene Sumeidiana; Yon Soepri Ondho; Edy Kurnianto
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.786 KB)

The twinning cow is presumable have difference in blood protein typology with the single cow. Thestudy investigated blood protein polymorphism in twinning and single of ongole grade and Simmentalcrossbred cows. The study used four twinning versus eight single ongole grade and seven twinning versussix single Simmental crossbred cows. Blood samples were analyzed by using marker ExactPro BroadRange (10-245kDa) and Sodium Dodecyl Sulphate-polyacrilamide Gel Electrophoresis method, to estimatethe loci of albumin (Alb), post-albumin (Pa), ceruloplasmin (Cp), transpherrin (Tf), and amylase-I (Amy-I).The genetic variation was analyzed based on the value of gene frequency, individual heterozigosity andaverage heterozigosity. In this study the twinning ongole grade cows had lower average heterozigosity(0.46) than the single ongole grade cows (0.49). The twinning Simmental crossbred cows had lower averageheterozigosity (0.35) than the single Simmental crossbred cows (0.40). The average heterozigosity ofongole grade cows had higher value than Simmental crossbred cows. The average heterozigosity rangedfrom 0.35-0.49, which was indicated high genetic variation in all cows. Higher gene frequency of allelealbumin A than albumin B influences the twinning trait in ongole grade and Simmental crossbred cows.

Polimorfisme Protein Plasma Darah pada Kelinci Rex, Lokal dan New Zealand White (POLYMORPHISM OF BLOOD PLASMA PROTEIN OF REX, LOCAL AND NEW ZEALAND WHITE RABBIT) Riri Sarfan; Sutopo Sutopo; Edy Kurnianto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The objective of this study was to determine genetic diversity of rabbit by blood plasma protein variance using electrophoresis at loci of pre-albumin (Pa), albumin (Alb), ceruloplasmin (Cp), transferrin (Tf), post-transferrin (P-tf) and amylase-I (Am-I). The blood analysis was performed at the Laboratory of Physiology and Biochemistry, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta. The materials used were 63 blood samples of rex, local and new zealand white (NZW) rabbit, in which the number of sample in each breed was 21, respectively. Materials were taken randomly. The protein bands resulted from electrophoresis were used to calculate the value of the gene frequency. Genetic diversity was determined by using individual heterozygosity (h) and the average heterozygosity (H) formulas. The genetic distance among breeds of rabbits was analyzed by using DISPAN and MEGA 6.06 programs. Chi-square (X2) was used to test of Hardy-Weinberg Law (HWL) equilibrium. The results showed that the loci of prealbumin (Pa), albumin (Alb), ceruloplasmin (Cp), transferrin (Tf), post-transferrin (P-tf), and amylase-I (Am-I) in rex, local and NZW rabbits were polymorphic. Rex and NZW rabbits had close genetic relationships based on phylogeny tree analysis. Chi-square test showed that rex, local, and NZW rabbits were in HWL equilibrium for locus of Pa and Cp, while at the locus of Alb, Tf, P-tf, and Am-I were in HWL unequilibrium. In conclusion, there are genetic variability of six locuses in rex, local, and NZW rabbits. ABSTRAK Tujuan penelitian ini adalah untuk mengetahui keragaman genetik kelinci melalui analisis keragaman protein plasma darah dengan metode elektroforesis pada lokus pre-albumin (Pa), albumin (Alb), ceruloplasmin (Cp), transferin (Tf), post-transferin (P-tf), dan amylase-I (Am-I). Analisis darah dilakukan di Laboratorium Fisiologi dan Biokimia, Fakultas Kedokteran Hewan, Universitas Gadjah Mada, Yogyakarta. Sebanyak 63 sampel darah kelinci rex, lokal dan New Zealand White (NZW) masing-masing sebanyak 21 sampel digunakan dalam penelitian ini. Penentuan kelinci dilakukan dengan metode observasional dengan cara kelinci dipilih secara acak. Pita-pita protein yang dihasilkan dari elektroforesis digunakan untuk menghitung nilai frekuensi gen. Perhitungan nilai ragam genetik ditentukan menggunakan rumus heterosigositas individual (h) dan rataan heterosigositas (H). Hubungan dan jarak genetik antar bangsa kelinci menggunakan program DISPAN dan MEGA 6.06. Analisis statistika menggunakan tabel X2 untuk menguji lokus-lokus dalam keseimbangan Hukum Hardy-Weinberg (HHW). Hasil penelitian menunjukkan bahwa lokus pre-albumin (Pa), albumin (Alb), ceruloplasmin (Cp), transferin (Tf), post-transferin (P-tf), dan amylase-I (Am-I) pada kelinci rex, lokal, dan NZW bersifat polimorfik. Kelinci rex dan NZW memiliki hubungan genetik yang dekat berdasarkan analisis pohon filogeni. Hasil uji X2 pada kelinci rex, lokal, dan NZW menunjukkan keseimbangan HHW pada lokus Pa dan Cp, sedangkan pada lokus Alb, Tf, P-tf, dan Amy-I memiliki keseimbangan HW yang berbeda-beda. Simpulan dari penelitian ini adalah terdapat keragaman genetik enam lokus pada kelinci rex, lokal, dan NZW.

Performa berahi sapi PO pada berbagai BCS yang disinkronisasi dengan medroxy progesteron acetate di Satker Sumberejo Kendal Muhammad Jamaluddin Ma'ruf; Edy Kurnianto; Sutiyono Sutiyono
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 27, No 2 (2017): Agustus
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2017.027.02.05

The objective of this study was to evaluate estrus appearance of Ongole Grade cattle on various Body Condition Score synchronized with medroxy progesterone asetat. Material used in this study was 68 head female Ongole Grade cattle and classified into 3 based on BCS, that was low (1-<4), midle (≥4-<7), high (≥7). Parameters of study was number of cattle showing estrus, appearance of vulva, mucus, uterus erection, speed and lenght of estrus. Collecting data was done since the synchronization was stopped and estrus detection was done at 8 am, 2 pm and 7 pm on 10 day. Data of this study was analized by descriptive mode and independent t-test. The result showed that cattle showing estrus in group of BCS 1-<4, ≥4-<7 and ≥7 were 32%, 35% and 40%, respectively. The mode of color and temperature changing on BCS 1-<4, ≥4-<7 and ≥7 were + (100%); vulva swelling each group was + (100%), + (77,78%), + and – (50%), respectively, mode of mucus all of groups were + (100%), while uterus erection at each group of BCS were +(100%), +(77,78%) and +(100%). Average of estrus speed on BCS 1-<4, ≥4-<7 and ≥7 were 364,6±117,65, 312,89±118,01 and 372±220,62, while estrus lenght BCS 1-<4, ≥4-<7 and ≥7 were 628,71±61,43, 533±190,19 and 422±121,62. In conclution, estrus synchronization did not give significant effect on various BCS. Various BCS showed that occurrence of estrus sign tended to same, but the middle BCS had better estrus length than that of small and high BCS.

Hubungan Indeks Bentuk Telur dan Surface Area Telur terhadap Bobot Telur, Bobot Tetas, Persentase Bobot Tetas dan Mortalitas Embrio pada Itik Pengging tituk suselowati; Edy Kurnianto; Sri Kismiati
Sains Peternakan: Jurnal Penelitian Ilmu Peternakan Vol 17, No 2 (2019): Sains Peternakan
Publisher : Universitas Sebelas Maret (UNS)

Tujuan penelitian ini adalah mengevaluasi bobot telur, bobot tetas, persentase bobot tetas dan mortalitas embrio berdasarkan ukuran indeks bentuk dan surface area telur itik Pengging. Penelitian menggunakan 1112 butir telur itik Pengging yang berasal dari 78 ekor jantan dan 772 ekor betina itik Pengging (nisbah perkawinan jantan : betina = 1:10). Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL). Perlakuan terdiri dari 3 kategori indeks bentuk telur atau surface area dengan 7 periode penetasan sebagai ulangan. Indeks bentuk adalah lonjong (68,78-78,93), normal (78,94-86,45) dan bulat (86,46-98,59) dan surface area adalah sempit (66,94-74,58 cm2), sedang (74,59-84,85 cm2) dan luas (84,86-110,70 cm2). Data yang diperoleh dianalisis menggunakan model klasifikasi satu arah dan regresi-korelasi antara indeks bentuk telur (X) dan surface area telur (Y). Hasil penelitian menunjukkan ada hubungan antara indeks bentuk telur dengan surface area telur, yang memiliki model persamaan regresi sederhana Y = 121,59998 – 0,50643X, R2 = 0,1376 dan berkorelasi negatif yaitu -0,37097. Hasil analisis ragam menunjukkan bahwa bobot telur, bobot tetas dan mortalitas total dipengaruhi oleh indeks bentuk telur maupun surface area telur (P<0,05). Mortalitas hari ke 8-25 tidak dipengaruhi oleh indeks bentuk telur, namun dipengaruhi oleh surface area telur (P<0,05). Persentase bobot tetas, mortalitas hari ke 1-7 dan hari ke 26-28 tidak dipengaruhi oleh indeks bentuk telur maupun surface area telur. Kesimpulan dari penelitian ini adalah indeks bentuk telur lonjong serta surface area telur luas dipilih untuk mendapatkan bobot telur, bobot tetas dan persentase bobot tetas yang tinggi serta mortalitas embrio yang rendah pada itik Pengging. Kata Kunci: Itik Pengging, Indeks bentuk, Surface area, Regresi, Korelasi, Produktivitas

Karakterisasi Morfometrik dan Pendugaan Jarak Genetik Kelinci New Zealand, Rex dan Flemish Giant Nida Fithrotun Nisa; Edy Kurnianto; Sutopo Sutopo
Jurnal Ilmu Ternak Vol 22, No 1 (2022)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jit.v22i1.39310

Tujuan penelitian untuk mengetahui spesifikasi ukuran morfometrik bagian kepala dan telinga antar bangsa kelinci New Zealand, Rex dan Flemish Giant dan menduga jarak genetik antar bangsa kelinci. Penelitian dilaksanakan tanggal 1 Agustus – 28 Oktober 2021 di daerah Jawa Tengah (Kabupaten Semarang, Kendal, Temanggung, Magelang) dan Yogyakarta. Materi yang digunakan 388 ekor kelinci, 229 ekor New Zealand, 99 ekor Rex dan 60 ekor Flemish Giant dengan kelompok umur yaitu (a) < 9 bulan; (b) 9 – 14 bulan; (c) 15 – 20 bulan; (d) > 20 bulan. Peneltian menggunakan alat yaitu pita ukur, ukur 0,1 cm dan jangka panjang yaitu 0,01 mm dan parameter yaitu panjang kepala, lebar kepala, panjang telinga dan lebar telinga. Data dianalisis dengan Analisis Multivariat pada Sistem Analisis Statistik (SAS) ver. Universitas dan MEGA 11 . Hasil analisis Komponen Utama menunjukkan parameter pembeda yaitu panjang kepala, lebar kepala, panjang telinga dan lebar telinga. Peta persebaran menunjukkan kelinci Rex berada di bawah axis X dan kiri axis Y, New Zealand berada di atas axis X dan di kiri axis Y, Flemish Giant berada di antara atas dan bawah axis X dan kanan axis Y. Nilai kesamaan yang besar pada kelinci Flemish Giant (98,33%,) New Zealand dan Rex tidak berbeda (82,10%) (81,82%). Jarak genetik terbesar kelinci Rex dengan Flemish Giant (38.015), kelinci New Zealand dengan Flemish Giant (32.394), jarak terkecil pada New Zealand dengan Rex (2.417).

Genetic Diversity of Simpo and Limpo Cattle Based on Five Blood-protein Locus Polymorphism Dela Ayu Lestari; Sutopo Sutopo; Asep Setiaji; Edy Kurnianto
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 3 (2022): JITRO, September 2022
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jitro.v9i3.25096

Simpo and Limpo cattle is a crossbred cattle of Indonesian local cattle and exotic cattle that popular among traditional farmers in Indonesia. The objective of this study was to identify genetic diversity of Simpo and Limpo cattle based on blood-protein polymorphism. A total of 70 blood samples were collected from unrelated 35 heads of Simpo cattle and 35 heads of Limpo cattle from Grobogan dan Kendal population. The blood plasm was used to identify the blood proteins. Blood protein analysis was performed using Sodium Dodecyl Sulfate-Polyacrilamide Gel Electrophoresis (SDS-PAGE). Observed bands were used to perform allele interpretation of five locus, namely Pre-albumin (Pe-Alb), Albumin (Alb), Post-albumin (Po-Alb), Ceruloplasmin (Cp) and Amylase-1 (Am-1). Results showed there were polymorphism in all of the five blood protein locus in both cattle; 4 of 5 and 2 of 5 of the blood-protein locus studied showed deviation from Hardy-Weimberg Equilibrium (HWE) for Simpo and Limpo cattle, respectively and the average heterozygosity value (H) was 0.478 and 0.362 for Simpo and Limpo cattle, respectively. In conclusion, the study indicated that population of Simpo and Limpo cattle has high genetic diversity. Keywords: allele frequency; genotype frequency; heterozygosity

Co-Authors Arista Seftiana Asep Setiaji Bagus Praditya Setyo Nugroho Dela Ayu Lestari Irene Sumeidiana Muhammad Jamaluddin Ma'ruf Nida Fithrotun Nisa Riri Sarfan Sri Kismiati Sutiyono Sutiyono Sutopo Sutopo tituk suselowati Tri Yuwono Yon Soepri Ondho

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