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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Ilmu Tanah dan Lingkungan (Journal of Soil Science and Environment) Microbiology Indonesia
Zainal Alim Mas'ud
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology

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 1 
Transfer Gen Badh2 Termutasi Varietas Aromatik Mentik Wangi ke Varietas Nonaromatik Ciherang Djarot Sasongko Hami Seno; Bambang Padmadi; Dewi Praptiwi; . Sugihartati; . Taufiq; Muhammad Taufan Fatahajudin; Helmy Ramadhan Al Anshary; Tri Joko Santoso; Zainal Alim Mas'ud
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 1 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Replacement of non-fragrant-native badh2 gene with mutated badh2 of fragrant rice is an alternative to engineer new fragrant rice varieties with good agronomic traits as those of non-fragrant. Fragrant gene (mutated badh2) of Mentik wangi donor was introgressed into non-fragrant Ciherang host through site-directed crossing. Mentik Wangi was crossed with Ciherang, and the progeny was further backcross until BC3F1. Bradbury marka-assisted PCR was used to select progeny in every cross and backcross generation. Ciherang, Mentik Wangi, and their cross/backcross showed different PCR profiles. The statues of badh2 gene (native/mutated), as well as alleles (homozygote/heterozygote) between samples were identified. Mutated badh2-introgression was also observed within the selected heterozygote cross or backcross progenies (Fl, BCl, BC2, and BC3), indicated successful transfer of mutated badh2 gene from donor to host.
Aplikasi Berbagai Marka Aromatik pada Varietas Padi Indonesia Djarot Sasongko Hami Seno; Satya Nugroho; Tri Joko Santoso; Dimas Adrianto; Dewi Praptiwi; Aniversari Apriana; Zainal Alim Mas'ud
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 3 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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This research applied various badh2.7 and badh2.2 fragrant markers (Bradbury et al., 2005b, Lang and Buu 2008, Shi et al., 2008, Sakthivel et al., 2009) on popular Indonesia non-fragrant (Ciherang, Fatmawati) and fragrant (Pandan wangi, Rojo Lele, Mentik Wangi, Gunung Perak, Pulu mandoti, Pare Kembang, Sintanur) rice varieties. For comparison, IR64, Nippon bare and Taipei 309 were included. Rice DNA samples were isolated from young  leaves, and PCR amplified using each of those fragrant markers. Results using all badh2.7 markers were consistently supported the existence of 2 group badh2.7 mutation pattern, while the use of badh2.2 marker indicated that there was no ex on 2 mutation. Badh2.7 sequence analysis of non-fragrant Ciherang, and aromatik member of group 1 (Pandan Wangi), as well as group 2 (Mentik Wangi) showed different mutation pattern. 
Identifikasi gen aroma pada progeni-progeni backcross antara varietas Ciherang dengan Pandan Wangi Djarot Sasongko Hami Seno; Akhmad Endang Zainal Hasan; Tri Joko Santoso; Bram Kusbiantoro; Zainal Alim Mas'ud
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 2 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Marker-assisted PCR has been considered as the most potential method for fragrant selection. RM223 is the only suitable marker to identify mutated badh2 gene of Pandan Wangi. This research applies RM223-assisted PCR in the introgression of fragrant gene (mutated badh2) of Pandan Wangi variety, to engineer non-transgenic fragrant variety with good agronomic traits as those of Ciherang.  Gene introduction was carried out through site-directed crossing; Pandan Wangi was crossed and backcrossed to Ciherang until heterozygot BC5F1, followed by selfing to obtain homozygot BC5F2.  RM223-assisted selection was conducted in each cross and backcross generation. RM223 was able to identify native, mutated and heterozygot badh2 of Ciherang, Pandan Wangi, and their cross/backcross progenies, respectively. Therefore, the introgression of mutated badh2 within progenies were observed, as well as the statues of badh2 gene (native/mutated) and alleles (homozygot/heterozygot). Further backcross and selfing to obtain BC5F2 is in progress.
PROFIL KELARUTAN LIMBAH MINYAK BERAT AKIBAT PENAMBAHAN BAHAN PENCAMPUR Charlena Charlena; Iswandi Anas; Zainal Alim Mas'ud; Ahmad Syahreza; Niken Dyah Wanodyanti
Jurnal Ilmu Tanah dan Lingkungan Vol 12 No 1 (2010): Jurnal Ilmu Tanah dan Lingkungan
Publisher : Departemen Ilmu Tanah dan Sumberdaya Lahan, Fakultas Pertanian, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.946 KB) | DOI: 10.29244/jitl.12.1.31-35

Abstract

Heavy Oil Waste (HOW) is on of the most important petroeum waste. In the recent years, many contamination of soil or water by HOW have been reported. Bioremediation is one of the alternative technology to clean the HOW contaminated soil since it is enviromentally friendly, effective, efficient and low cost. The contaminated soil is diluted in water to form bioslurry. However, the solubility of HOW in water is very low. To increase HOW solubility in water, addition of four mixing agents were tested namely: Sodium Tripolyphosphate (STPP), Carboxymethyl cellulose (CMC), quart sand and pumice stone. The main characteristics of HOW were solid TPH was 17.2%, water content was 1.96% and Cu and Hg content was 1.49 ppm and 3.33 ppb, respectively. The parameters determined were turbidity, solid TPH, liquid TPH pH and COD. The results showed that (1) the highest turbidity was in the STPP addition; (2) the lowest solid TPH was in the addition of pumice stone; (3) the highest liquid TPH was in the STPP addition;(4) the pH varied from 3.6 to 7.9; (5) the ghigest COD was in the addition of STPP. From these results it can be concluded that the best mixing agent to increase the solubility of HOW in soil slurry was STPP. Keywords: heavy oil waste (HOW), mixing agents, solubility of HOW
Isolation and Characterization of Antimicrobial Substance from Marine Streptomyces sp. ROFIQ SUNARYANTO; BAMBANG MARWOTO; TUN TEDJA IRAWADI; ZAINAL ALIM MAS'UD; LIESBETINI HARTOTO
Microbiology Indonesia Vol. 4 No. 2 (2010): August 2010
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6996.369 KB) | DOI: 10.5454/mi.4.2.7

Abstract

Isolation and purification of antimicrobial active substance produced by marine Actinomycetes has been carried out. Marine sediment samples were obtained from six different places at Banten West Coast. Isolation was conducted using two pretreatment methods, acid and heat shock pre-treatment. A total of 29 Actinomycetes isolates were obtained from the various sediment samples collected, then tested for antimicrobial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Pseudomonas aeruginosa ATCC27853, Bacillus subtilis ATCC 66923, Candida albicans BIOMCC00122, and Aspergillus niger BIOMCC00134. Among the isolates, isolate A11 was the most activity to Gram-positive and Gram-negative bacteria, and morphological observation and identification using 16S rRNA showed that the isolate was similar to Streptomyces sp. Production of active compound from A11 isolate used yeast peptone medium. Purification of active compounds was carried out using silica-gel-column chromatography and preparative HPLC. A single peak of active compounds was detected by HPLC, which showed a retention time of 8.35 min and maximum absorbance in UV visible at 210 nm and 274.5 nm respectively.
Co-Authors . Sugihartati . Taufiq Ahmad Syahreza Akhmad Endang Zainal Hasan Aniversari Apriana BAMBANG MARWOTO Bambang Padmadi Bram Kusbiantoro Charlena Dewi Praptiwi Dimas Adrianto Djarot Sasongko Hami Seno Helmy Ramadhan Al Anshary Iswandi Anas Liesbetini Hartoto Muhammad Taufan Fatahajudin Niken Dyah Wanodyanti Rofiq Sunaryanto Satya Nugroho Tri Joko Santoso Tun Tedja Irawadi