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All Journal HAYATI Journal of Biosciences Majalah Patologi Indonesia The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy
Budiningsih Siregar
Department of Anatomical Pathology, Faculty of Medicine, University of Indonesia, Jakarta 10430, Indonesia
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Health Professions Medicine & Pharmacology Public Health

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Hubungan antara Ekspresi MMP-2, C-erbB-2 (HER-2/neu), dan Derajad Keganasan Histologik Karsinoma Endometrium dengan Kedalaman Invasi Miometrium Resti Arania; Budiningsih Siregar; Esti Dwi Sabarati
Majalah Patologi Indonesia Vol 19 No 2 (2010): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (420.266 KB)

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ABSTRAKLatar belakangKedalaman invasi miometrium pada karsinoma endometrium penting dalam menentukan stadium dan prognosis.Saat ini telah banyak ditemukan faktor prognostik yang berhubungan dengan perubahan molekuler padakarsinogenesis, antara lain Matrix metalloproteinase-2 (MMP-2) yang merupakan enzim proteolitik yangberperan dalam degradasi matriks ekstrasel pada proses invasi dan c-erbB2, suatu reseptor faktor pertumbuhanyang bila ekspresinya meningkat berhubungan dengan prognosis buruk dan derajat keganasan histologik tinggi.Penelitian ini bertujuan untuk mengetahui hubungan antara ekspresi MMP-2, c-erbB2 dan derajad keganasanhistologik dengan kedalaman invasi miometrium pada karsinoma endometrium.CaraDilakukan studi retrospektif potong lintang terhadap 28 spesimen hasil operasi karsinoma operabel diDepartemen Patologi Anatomik FKUI/RSUPNCM dalam kurun waktu 2002-2008. Dilakukan pemeriksaan ulangpreparat HE untuk menilai jenis histologik, derajad keganasan dan kedalaman invasi Ekspresi MMP-2 dan cerbB2 dinilai dari pulasan imunohistokimia menggunakan antibodi monoklonal. Hasilnya dibandingkan menurutkedalaman invasi dan hubungan antar variabel.Hasil dan diskusiTerdapat hubungan yang bermakna antara derajad keganasan histologik dengan kedalaman invasi miometrium(p=0,02) yaitu kanker dengan derajad keganasan sedang-tinggi berisiko terjadi invasi lebih dari setengahketebalan miometrium 9 kali. Terdapat perbedaan bermakna antara ekspresi MMP-2 dengan derajad keganasanhistologik. Ekspresi MMP-2 lebih cenderung pada derajad keganasan rendah. Tidak terdapat hubungan antaraekspresi MMP-2, dan ekspresi c-erbB2 dengan kedalaman invansi. Dari penelitian ini disimpulkan bahwaderajad keganasan berhubungan dengan kedalaman invasi miometrium sedangkan ekspresi MMP-2 maupun cerbB2 tidak berhubungan.
Correlation Between Akt and p53 Protein Expression and Chemoradiotherapy Response in Cervical Cancer Patients IIN KURNIA; BUDININGSIH SIREGAR; SETIAWAN SOETOPO; IRWAN RAMLI; TJAHYA KURJANA; . ANDRIONO; MARINGAN DIAPARI LUMBAN TOBING; BETHY SURYAWATHI; TEJA KISNANTO; DEVITA TETRIANA
HAYATI Journal of Biosciences Vol. 21 No. 4 (2014): December 2014
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1416.842 KB) | DOI: 10.4308/hjb.21.4.173

Abstract

Akt is a protein that is associated with cell proliferation and is expressed at high levels in cancer cells. Some research indicates it may play a role in increasing the resistance of cancer cells to chemotherapy treatment. P53 is a tumor suppressor protein that influences the cell cycle and apoptosis. The purpose of this study was to examine the relationship between the expression of Akt and p53 in cancerous tissue before chemoradiation treatment, and the clinical response to treatment of cervical cancer patients. Twenty microscopic tissue samples were taken from cervical cancer biopsies obtained from patients before cancer treatment. The tissue samples were stained with p53 and Akt antibodies via immunohistochemistry technique, to measure expression of both proteins. After completion of chemoradiotherapy, patients’ clinical response to treatment was determined using the pelvic control method. Our results revealed no correlation between expression of Akt and p53 index (P = 0.74) as well as between p53 Index and chemoradiotherapy clinical response (P=0.29). There was significant correlation between expression of Akt and cervical cancer chemoradiotherapy response (P = 0.03). There was no correlation found between p53 index and chemoradiotherapy clinical response (P = 0.29). High expression of Akt may related with high cell proliferation and resistance to chemoradiotherapy.
Alteration of Subcellular Beta Catenin Expression in Normal Mucosa Adenoma and Carcinoma in Relation to Colorectal Carcinogenesis Pamela Damaledo Abineno; Diah Rini Handjari; Budiningsih Siregar
The Indonesian Journal of Gastroenterology, Hepatology, and Digestive Endoscopy VOLUME 11, NUMBER 2, August 2010
Publisher : The Indonesian Society for Digestive Endoscopy

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24871/112201083-87

Abstract

Background: Adenomatous polyposis coli (APC) gene mutation was found in up to 80% of cases of sporadic colorectal cancers and adenomas. Loss of APC protein function has been known as one of the early process in colorectal carcinogenesis. This event leads to the accumulation of beta catenin in the cytoplasm and nucleus and subsequently activates target genes that regulate cell proliferation and apoptosis. The aim of this study was to investigate the alteration of subcellular beta catenin expression in the progression of colorectal cancer. Method: This cross-sectional study was conducted with 30 paraffin-embedded tissue sections each of normal colorectal mucosa, adenomas and carcinomas. Alteration of beta catenin expression in membranous, cytoplasmic, and nuclear compartments were evaluated by immunohistochemical staining. Results: Beta catenin immunoreactivity was detected in all cases, of which 87 (96.7%) cases showed membranous expression, 78 (86.7%) cases had cytoplasmic and 51 cases (56.7%) had nuclear expression. Such results were statistically significant (p 0.000). All normal colorectal epithelium showed membranous beta catenin expression with 18 (60.0%) cases showed cytoplasmic and no nuclear beta catenin expression was found. Strong cytoplasmic expression was found in 17 (56.7%) adenomas and 25 (83.3%) carcinomas; while strong nuclear expression was found in 12 (40.0%) adenomas and 17 (56.7%) carcinomas. There was no statistical significant association between beta catenin expression in the membranous, cytoplasmic and nuclear compartment with the degree of dysplasia or differentiation of tumor (p 0.05). Conclusion: Altered subcellular expression of beta catenin occurs as the oncogenic process develops from adenoma into carcinoma. Such finding reflects the importance of beta-catenin in colorectal carcinogenesis. Keywords: beta catenin, colorectal cancer, adenoma, colorectal cancer progression
Co-Authors . ANDRIONO BETHY SURYAWATHI Devita Tetriana Diah Rini Handjari Esti Dwi Sabarati IIN KURNIA Ketut Tirtayasa MARINGAN DIAPARI LUMBAN TOBING Pamela Damaledo Abineno Resti Arania SETIAWAN SOETOPO Teja Kisnanto TJAHYA KURJANA