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KEMAMPUAN EKSTRAK SENYAWA AKTIF BAKTERI ENDOFIT DALAM MENGHAMBAT PERTUMBUHAN Fusarium oxysporum f.sp. PADA KELAPA SAWIT Candrawati, Emilia; Rupaedah, Bedah; Sumpono, Sumpono; Sundaryono, Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 2 (2018): December 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (921.358 KB) | DOI: 10.29122/jbbi.v5i2.2769

Abstract

Ability of Active Compound Extract of Endophytic Bacteria to Inhibit the Growth of Fusarium oxysporum f.sp. in Oil Palm ABSTRACTWilt vessels disease in oil palm plants is caused by Fusarium oxysporum f.sp. This disease is very harmful because of its ability to kill the infected oil palm plant in less than a year. Endophytic bacteria are likely to be biological controllers for the disease because of their ability to produce bioactive antifungal compounds. Isolation of endophytic bacteria from oil palm plant and activity test of their active compounds against F. oxysporum f.sp. in vitro had been done. Antagonistic test of endophytic bacterial isolates against F. oxysporum f.sp. was carried out using a double culture method. The potential endophytic bacterial isolates were extracted using ethyl acetate solvent for their active compounds, which were then tested for its activity in inhibiting the growth of F. oxysporum f.sp. The results showed that the active compound extract of B11 endophytic bacteria with the incubation time of 24 and 54 hours gave the growth inhibition of F. oxysporum f.sp. at the level of 29.23% and 43.85%, respectively.Keywords: antagonistic test, bioactive compound, endophytic bacteria, F. oxysporum f.sp., oil palm ABSTRAKPenyakit layu pembuluh pada tanaman kelapa sawit disebabkan oleh Fusarium oxysporum f.sp. Penyakit ini menjadi penyebab kematian tanaman kelapa sawit yang telah terinfeksi dalam waktu kurang dari setahun. Bakteri endofit asal tanaman kelapa sawit dimungkinkan menjadi pengendali hayati bagi penyakit ini karena kemampuan bakteri tersebut memproduksi senyawa bioaktif yang bersifat antifungi. Isolasi bakteri endofit dari tanaman kelapa sawit dan uji aktivitas senyawa aktifnya terhadap F. oxysporum f.sp. secara in vitro telah dilakukan. Uji antagonis isolat bakteri endofit terhadap jamur patogen F. oxysporum f.sp. menggunakan metode kultur ganda. Isolat bakteri endofit potensial diekstrak senyawa aktifnya dengan menggunakan pelarut etil asetat, kemudian senyawa aktif ini diuji aktivitasnya dalam menghambat pertumbuhan jamur patogen F. oxysporum f.sp. Hasil penelitian menunjukkan bahwa ekstrak senyawa aktif bakteri endofit B11 dengan waktu inkubasi 24 dan 54 jam memberikan daya hambat terhadap F. oxysporum f.sp. sebesar masing-masing 29,23% dan 43,85%.Kata Kunci: bakteri endofit, F. oxysporum f.sp., kelapa sawit, senyawa aktif, uji antagonis 
KEMAMPUAN EKSTRAK SENYAWA AKTIF BAKTERI ENDOFIT DALAM MENGHAMBAT PERTUMBUHAN Fusarium oxysporum f.sp. PADA KELAPA SAWIT Candrawati, Emilia; Rupaedah, Bedah; Sumpono, Sumpono; Sundaryono, Agus
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (921.358 KB) | DOI: 10.29122/jbbi.v5i2.2769

Abstract

Ability of Active Compound Extract of Endophytic Bacteria to Inhibit the Growth of Fusarium oxysporum f.sp. in Oil Palm ABSTRACTWilt vessels disease in oil palm plants is caused by Fusarium oxysporum f.sp. This disease is very harmful because of its ability to kill the infected oil palm plant in less than a year. Endophytic bacteria are likely to be biological controllers for the disease because of their ability to produce bioactive antifungal compounds. Isolation of endophytic bacteria from oil palm plant and activity test of their active compounds against F. oxysporum f.sp. in vitro had been done. Antagonistic test of endophytic bacterial isolates against F. oxysporum f.sp. was carried out using a double culture method. The potential endophytic bacterial isolates were extracted using ethyl acetate solvent for their active compounds, which were then tested for its activity in inhibiting the growth of F. oxysporum f.sp. The results showed that the active compound extract of B11 endophytic bacteria with the incubation time of 24 and 54 hours gave the growth inhibition of F. oxysporum f.sp. at the level of 29.23% and 43.85%, respectively.Keywords: antagonistic test, bioactive compound, endophytic bacteria, F. oxysporum f.sp., oil palm ABSTRAKPenyakit layu pembuluh pada tanaman kelapa sawit disebabkan oleh Fusarium oxysporum f.sp. Penyakit ini menjadi penyebab kematian tanaman kelapa sawit yang telah terinfeksi dalam waktu kurang dari setahun. Bakteri endofit asal tanaman kelapa sawit dimungkinkan menjadi pengendali hayati bagi penyakit ini karena kemampuan bakteri tersebut memproduksi senyawa bioaktif yang bersifat antifungi. Isolasi bakteri endofit dari tanaman kelapa sawit dan uji aktivitas senyawa aktifnya terhadap F. oxysporum f.sp. secara in vitro telah dilakukan. Uji antagonis isolat bakteri endofit terhadap jamur patogen F. oxysporum f.sp. menggunakan metode kultur ganda. Isolat bakteri endofit potensial diekstrak senyawa aktifnya dengan menggunakan pelarut etil asetat, kemudian senyawa aktif ini diuji aktivitasnya dalam menghambat pertumbuhan jamur patogen F. oxysporum f.sp. Hasil penelitian menunjukkan bahwa ekstrak senyawa aktif bakteri endofit B11 dengan waktu inkubasi 24 dan 54 jam memberikan daya hambat terhadap F. oxysporum f.sp. sebesar masing-masing 29,23% dan 43,85%.Kata Kunci: bakteri endofit, F. oxysporum f.sp., kelapa sawit, senyawa aktif, uji antagonis 
PREPARATION OF LIQUID HIDROCARBON FUEL BY CRACKING Agus Sundaryono dan Budiyanto
Jurnal Teknologi Industri Pertanian Vol. 20 No. 1 (2010): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

 ABSTRACT The objective of the study was to evaluate the utilization of two types of zeolite catalists to produce liquid hidrocarbon fuel from oil fraction of palm oil mill effluent (POME).  The oil fractions of POME  were converted into metil ester prior to be cracked using bifunctional catalist of zeolite.  Esterification of the oil was done by using  H2SO4  at  60 oC for two hours and transesterification of it by using NaOH at  55  oC.  The product methtyl ester of the POME was  cracked with zeolit catalists Cr-H-ZA (25:1) and Ni-H-ZA.  The result had indicated that zeolit catalist Cr-H-ZA could yield liquid hidrocarbon that containing fraction of gasoline fuel better than zeolite catalist Ni-H-ZA. The product liquid hidrocarbon had kinematic viscosity of 0.8329 cSt and had similar properties of gasoline fuel.  Keywords:  catalitic craking, liquid hidrocarbon fuel, methylester, POME
CHARACTERISTIC OF BIODISESEL AND BIODIESEL BLENDING OF OIL LOSSES FROM LIQUID WASTE OF OIL PALM FACTORY Agus Sundaryono TIN
Jurnal Teknologi Industri Pertanian Vol. 21 No. 1 (2011): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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ABSTRACT  The aim of this study was to convert oil losses from palm oil mill (POM) liquid waste into methyl ester. Methyl ester as a result of conversion process was further developed into blending biodiesel. The research was conducted through several stages: 1). insolating oil losses of POM waste which taken from PT Bio Nusantara Bengkulu. Oil losses were degummed and bleached and then the free fatty acid was determined. 2). the conversion process  of oil losses into methyl ester was done through two stages of esterification reactions; using H2SO4 catalyst at 60oC for 2 hours and transesterification using NaOH catalyst at 55oC for 2 hours. 3). Methyl ester was developed as a blending  of biodiesel,  which is a mixture of methyl ester with diesel oil, with the percentages of methyl ester of 25%, 30%, 35%, 40%, and 45%. The yield of methyl ester obtained was 87%. Characterization of methyl ester was done with the following results : 0.858 g/cm3 density, 3.04 cSt viscosity, 3.5% water content, 12.3°C cloud point, 8.3°C pour point, 6.86 mgNaOH/g acid number, 164.4 mgNaOH/g saponification numbers, 108.4% iodine number, and 51.58 cetane number. The parameters do not meet SNI 04-7182-2006 for biodiesel were water  content and acid number. Methyl ester can be developed into blending biodiesel with the characteristic of density, viscosity, cloud point, pour point, saponification number, and cetane number which meet biodiesel standard according to SNI 04-7182-2006.  Keywords: biodiesel, oil losses, methyl ester, waste palm oil mill (POM), transesterification  
PERENGKAHAN KATALITIK METIL ESTER DARI MINYAK LIMBAH CAIR PABRIK MINYAK KELAPA SAWIT DENGAN KATALIS Cr/Mo/HZA DAN Ni/Mo/HZA Agus Sundaryono, Dewi Handayani, Budiman , Sherly Winda TIP
Jurnal Teknologi Industri Pertanian Vol. 25 No. 1 (2015): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

The proposed catalytic cracking technology leads to the production of hydrocarbons that are present in variousfractions of gasoline, kerosene and diesel. In this study, the methyl ester was converted by catalytic cracking using catalyst Cr / Mo / HZA and Ni / Mo / HZA. Preparation of catalyst Cr / Mo / HZA was performed by soaking the natural zeolite powder (ZA) with HCl. The catalyst of HZA was refluxed by (NH4)Mo7O24.4H2O, then dried to obtain the catalyst of Mo/HZA. Futhermore,catalyst of Mo / HZA was refluxed by Cr (NO3) 3.9H2O. The catalyst pruduct of Cr / Mo /HZA was calcined at 500ºC. Catalysts Ni/Mo/ HZA was obtained through the same procedure by replacing Cr (NO3)3.9H2O with NiCl2.6H2O. Cracking was done by refluxing a methyl ester of oil residu slude waste (POME) with 1.5 g catalyst Cr / Mo / HZA and catalyst Ni / Mo / HZA at 350-380ºC . Results of the reflux were analyzed by GC-MS. The products of cracking of catalysts Cr / Mo / HZA were hydrocarbons that wasfractions of keroseneof 31.35%, and diesel fraction of 10.16%. While the product catalyst of Ni / Mo / HZA were 0.33% gas fractions,10.63% fractions gasoline and 11.02%fractions kerosene. The density was similar to densities ofgasoline and kerosene, the viscosity was similar to kerosene, acid number was about two times of acid numbers of gasoline and kerosene while water content was still higher than that of gasoline and kerosene.Keywords: Methyl ester, catalytic cracking, palm oil, gasoline, kerosene
KONVERSI KONVERSI BIOGASOLIN DARI RESIDU MINYAK PALM OIL MILLS EFFLUENT (POME) DENGAN BANTUAN IRADIASI ULTRASONIK DAN PERENGKAHAN KATALITIK Agus Sundaryono,Arsela Eko Listiono, M. Lutfi Firdaus, Dewi Handayani
Jurnal Teknologi Industri Pertanian Vol. 28 No. 1 (2018): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

Palm oil mill effluent (POME) is a liquid waste by product of fresh fruit bunches processing (FFB) in palm oil factory (POF). POME contains oil residue of 0.7% (from treated FFB). The purpose of this study was to convert oil residue of POME into biogasolin with the help of ultrasonic irradiation and catalytic cracking. The convertion was performed by reacting oil residue of POME with methanol in an esterification and transesterification reaction with the aid of ultrasonic irradiation. The reaction product was further reacted in the catalytic cracking reaction using zeolite-based catalysts (Ni/HZa and Mo-Ni/HZa). The overall reaction results were characterized using GCMS to determine the constituent components. The components  found using Mo-Ni/HZa catalyst were 51.22% biogasoline and other products of kerosene and diesel as well as long chain methyl ester components, whereas using Ni/HZa catalyst was found a component equivalent to diesel at 2.44%.Keywords: biogasoline, ultrasonic irradiation, catalytic cracking, zeolite catalyst, POME
KEMAMPUAN EKSTRAK SENYAWA AKTIF BAKTERI ENDOFIT DALAM MENGHAMBAT PERTUMBUHAN Fusarium oxysporum f.sp. PADA KELAPA SAWIT Emilia Candrawati; Bedah Rupaedah; Sumpono Sumpono; Agus Sundaryono
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 2 (2018): December 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (921.358 KB) | DOI: 10.29122/jbbi.v5i2.2769

Abstract

Ability of Active Compound Extract of Endophytic Bacteria to Inhibit the Growth of Fusarium oxysporum f.sp. in Oil Palm ABSTRACTWilt vessels disease in oil palm plants is caused by Fusarium oxysporum f.sp. This disease is very harmful because of its ability to kill the infected oil palm plant in less than a year. Endophytic bacteria are likely to be biological controllers for the disease because of their ability to produce bioactive antifungal compounds. Isolation of endophytic bacteria from oil palm plant and activity test of their active compounds against F. oxysporum f.sp. in vitro had been done. Antagonistic test of endophytic bacterial isolates against F. oxysporum f.sp. was carried out using a double culture method. The potential endophytic bacterial isolates were extracted using ethyl acetate solvent for their active compounds, which were then tested for its activity in inhibiting the growth of F. oxysporum f.sp. The results showed that the active compound extract of B11 endophytic bacteria with the incubation time of 24 and 54 hours gave the growth inhibition of F. oxysporum f.sp. at the level of 29.23% and 43.85%, respectively.Keywords: antagonistic test, bioactive compound, endophytic bacteria, F. oxysporum f.sp., oil palm ABSTRAKPenyakit layu pembuluh pada tanaman kelapa sawit disebabkan oleh Fusarium oxysporum f.sp. Penyakit ini menjadi penyebab kematian tanaman kelapa sawit yang telah terinfeksi dalam waktu kurang dari setahun. Bakteri endofit asal tanaman kelapa sawit dimungkinkan menjadi pengendali hayati bagi penyakit ini karena kemampuan bakteri tersebut memproduksi senyawa bioaktif yang bersifat antifungi. Isolasi bakteri endofit dari tanaman kelapa sawit dan uji aktivitas senyawa aktifnya terhadap F. oxysporum f.sp. secara in vitro telah dilakukan. Uji antagonis isolat bakteri endofit terhadap jamur patogen F. oxysporum f.sp. menggunakan metode kultur ganda. Isolat bakteri endofit potensial diekstrak senyawa aktifnya dengan menggunakan pelarut etil asetat, kemudian senyawa aktif ini diuji aktivitasnya dalam menghambat pertumbuhan jamur patogen F. oxysporum f.sp. Hasil penelitian menunjukkan bahwa ekstrak senyawa aktif bakteri endofit B11 dengan waktu inkubasi 24 dan 54 jam memberikan daya hambat terhadap F. oxysporum f.sp. sebesar masing-masing 29,23% dan 43,85%.Kata Kunci: bakteri endofit, F. oxysporum f.sp., kelapa sawit, senyawa aktif, uji antagonis 
Pembuatan Nanopartikel Perak yang Ramah Lingkungan Beserta Aplikasinya untuk Mendeteksi Ion Merkuri Secara Citra Digital M. Lutfi Firdaus; Hadi Apriyonda; Elvinawati Elvinawati; Eko Swistoro; Agus Sundaryono
al Kimiya: Jurnal Ilmu Kimia dan Terapan Vol 6, No 2 (2019): al Kimiya: Jurnal Ilmu Kimia dan Terapan
Publisher : Department of Chemistry, Faculty of Science and Technology, UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/ak.v6i2.6487

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Dewasa ini, nanopartikel mendapatkan perhatian istimewa di kalangan para peneliti termasuk pemerhati lingkungan karena nanopartikel memiliki luas permukaan yang sangat besar sehingga bisa dimanfaatkan lebih lanjut untuk berbagai keperluan. Penelitian ini bertujuan untuk membuat nanopartikel perak secara biosintesis menggunakan ekstrak buah anggur beserta aplikasinya sebagai pereaksi dalam mendeteksi logam berat berbahaya, khususnya merkuri (II). Buah anggur diekstraksi menggunakan air pada suhu 80°C. Setelah dicampur dengan prekursor perak nitrat, reaksi pembentukan nanopartikel perak dipercepat dengan memanfaatkan sinar matahari sebagai energi panas alami. Nanopartikel perak yang terbentuk memiliki puncak absorbansi pada panjang gelombang maksimum 420 nm, dan selektif terhadap ion merkuri (II), yang ditandai dengan berubahnya warna nanopartikel perak tersebut dari coklat kekuningan menjadi bening. Selanjutnya, kamera digital dimanfaatkan untuk menganalisis konsentrasi ion merkuri di dalam sampel. Keakuratan metode citra digital ditentukan dengan membandingkan hasil pengukurannya terhadap metode Spektrofotometri UV-Vis. Secara umum, metode citra digital dengan nanopartikel perak sebagai reagen deteksi ion merkuri dapat digunakan dengan kinerja mumpuni. Konsentrasi ion Hg (II) dari sampel lingkungan yang dianalisis menggunakan metode yang dikembangkan ini berada di bawah ambang batas yang diperbolehkan oleh Permenkes RI No. 492 tahun 2010, sehingga air lingkungan yang dianalisis masih layak dipergunakan untuk kebutuhan rumah tangga.
THE IMPLEMENTATION OF A FLIPPED CLASSROOM MODEL UTILIZING A SCIENTIFIC APPROACH AND FLIPBOOK MAKER E-MODULE TO IMPROVE STUDENT LEARNING OUTCOMES Dewi Handayani; Endang Widi Winarni; Agus Sundaryono; Muhammad Lutfi Firdaus; Muzanip Alperi
Erudio Journal of Educational Innovation Vol 8, No 1 (2021): Erudio Journal of Educational Innovation
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18551/erudio.8-1.7

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This research aimed to determine the influence of the flipped classroom model on students learning outcomes on the chemistry subject, particularly on electrolyte and non-electrolyte solutions. This research employed pre-experimental research and a one-group pre-test post-test design on one sample group. We used the method to compare pre-test and post-test results. This research was conducted in Public High School 9, Bengkulu City, Bengkulu Province, Indonesia. The population was 76 tenth graders of Mathematics and Natural Science Class. As many as 25 students of Class X-I were chosen as the samples using purposive sampling. The research instrument was pre-test and post-test, consisting of 10 questions to assess learning outcomes. We analyzed the average value of learning outcomes. The SPSS application was used to evaluate the normality, homogeneity, and hypotheses. The average pre-test score was 64.6, and the average post-test score was 87.8. Learning outcomes were normally and homogeneously distributed. The hypothesis testing produced a significance value of 0.000<0.05. Therefore, the flipped classroom model using a scientific approach and e-modules significantly affected the learning outcomes of the chemistry subject. The research result showed that the implementation of the flipped classroom model positively affected and improved the learning outcomes on the chemistry subject, particularly on electrolyte and non-electrolyte solutions.  
Perubahan Kandungan Β-Karoten, Asam Lemak Bebas dan Bilangan Peroksida Minyak Sawit Merah Selama Pemanasan Budiyanto Budiyanto; Devi Silsia; Zulman Efendi; Rasie Janika
agriTECH Vol 30, No 2 (2010)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (694.699 KB) | DOI: 10.22146/agritech.9676

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The objectives of the study were to evaluate decreasing pattern of ß-carotene, determine the changes of free fatty acid content and to determine peroxide value of red plam olein oil during heating. Red palm olein oil samples were heated at four different temperatures (150°C, 160°C, 170°C, and 180°C) for ten hours. Heated samples were taken every 30 minutes for each heating treatment. The ß-carotene, FFA, and peroxide value in each sample were evaluated. The data were plotted into its curve for further evaluation. The finding indicated that ß-carotene content in red palm olein oil decreased sharply in the first three hours of heating, especially for the oil heated at 180°C.  In adition, heating for 10 hours resulted in decreasing of FFA and peroxide values, especially for red palm oil samples heated above 150°C.ABSTRAKPenelitian ini bertujuan untuk mengkaji pola penurunan kandungan ß-karoten, menentukan pola perubahan kandun- gan asam lemak bebas (FFA), dan menentukan pola perubahan bilangan peroksida minyak sawit merah selama pe- manasan. Minyak sawit merah dipanaskan pada empat suhu pemanasan yang berbeda  (150°C, 160°C, 170°C, dan180°C) selama 10 jam. Sampel minyak diambil setiap 30 menit dari setiap  minyak yang dipanaskan. Kandungan ß-karoten, FFA, dan bilangan peroksida pada setiap sampel dievaluasi. Data yang diperoleh dituangkan secara gra- fis untuk analisa lebih lanjut. Hasil Penelitian menunjukkan kandungan ß-karoten minyak sawit merah mengalami penurunan tajam selama tiga jam pertama pemanasan, khususnya pada minyak yang dipanaskan pada suhu 180°C. Selain itu, lama pemanasan selama 10 jam mengakibatkan terhadap turunnya kadar FFA serta penurunan bilangan peroksida,  khususnya pada minyak yang mengalami pemanasan lebih dari 150°C.
Co-Authors ACENG RUYANI Ade Indriani Putri Alfia Novera Indah Esa Kasih Arina Fatharani Arsela Eko Listiono Bedah Rupaedah Bedah Rupaedah, Bedah Bhakti Karyadi Bhakti Karyadi Bhakti Karyadi Bonodikun Bonodikun Candrawati, Emilia Candrawati, Emilia Catur Herison Chyntia Margareta Cici Romayanti Dapot Parulian Manurung Devi Silsia Devi Silsia Dewi Handayani Dhea Prasiwi Dian Kartika Sari Eko Swistoro Elvinawati Elvinawati Emi Hartati Emilia Candrawati Emilia Candrawati Endang Widi Winarni Endang Widi Winarni Ernis, Gustria Euis Nursaa'adah Euis Nursaadah Euis Nursa’adah Fahrurrozi, Fahrurrozi Fenny Andriani Fitri Yuwita Fuji Ramadenti Hadi Apriyoanda Hadi Apriyonda Hermansyah Amir I Nyoman Candra I Nyoman Chandra Ilham Agus Saputra Jertadi Putri Dhani Lina Widawati M Lutfi Firdaus M. Farid M. Lutfi Firdaus M. Lutfi Firdaus Maya Wahyunarti Mayshah Purnamasari Megasari Megasari Meizul Zuki Mellyta Uliyadari Mina Sihite Hutasoit Muchammad Farid Mukhsin Mukhsin Muzanip Alperi Nur Wakidatul Khasanah Nurhamidah Nurhamidah Nurhamidah Nurhamidah Nurhamidah Nurhamidah Nurleli Nurleli Nurlia Latifah Pandu Imam Sudibyo Adib Pandu Imam Sudibyo Adib Pramesthi Regita Putri Putri Suci Asriani Rantika Khumairah Rasie Janika Reni Jumika Ridwan Yahya Rika Partika Sari Rina Elvia Rina Elvia Rina Endriani Ririn Trinanda Rofiqa Asri Sari Puspita Ningsih Shiba Meike Indira Shinta Lestari Sipriyadi Sri Astuti Sumpono Sumpono Sumpono Sumpono Sumpono, Sumpono Suryadi Suryadi Theo Kuntara Tuti Maryana Tyas Sri Muryati Ulfah Anis Umi kalsum Wuri Marsigit Yenni Okfrianti Yohannes Adimar Marbun Yulia Suparti Zulman Efendi