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All Journal Jurnal Ilmu Pertanian Indonesia FORUM STATISTIKA DAN KOMPUTASI Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Kimia (Journal of Chemistry) International Journal of Renewable Energy Development SAINS DAN MATEMATIKA JURNAL KIMIA SAINS DAN APLIKASI Jurnal Ilmu Lingkungan The Journal of Pure and Applied Chemistry Research agriTECH INDONESIAN JOURNAL OF PHARMACY Journal of Information System Indonesian Journal of Chemical Science Indonesian Journal of Chemistry Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan Jurnal Ilmiah Pesantren Jurnal Ilmu dan Teknologi Kayu Tropis JURNAL ILMU KEFARMASIAN INDONESIA Jurnal Jamu Indonesia Advances in Food Science, Sustainable Agriculture and Agroindustrial Engineering (AFSSAAE) Media Agribisnis RESEARCH AND DEVELOPMENT JOURNAL OF EDUCATION (RDJE) Jurnal Pengabdian Polinema Kepada Masyarakat Prosiding Seminar Nasional Sasindo Jurnal Ilmiah Berkala: Sains dan Terapan Kimia Dinamika Kesehatan: Jurnal Kebidanan dan Keperawatan

Mohamad Rafi

Departemen Kimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor, Bogor

Author-ID : 2450722

Agriculture, Biological Sciences & Forestry Humanities Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Antioxidant Capacity, Phytochemical Profile, and Clustering of Pomegranate (Punica granatum L.) Peel Extracts Using Different Solvent Extraction Rafi, Mohamad; Wulansari, Laela; Septaningsih, Dewi Anggraini; Purnomo, Tsania Firqin; Auliatifani, Reza; Khaydanur, Khaydanur; Ilmiawati, Auliya; Yulianti, Wina; Nengsih, Nunuk Kurniati; Suparto, Irma Herawati; Kusuma, Wisnu Ananta
Journal of Tropical Life Science Vol 11, No 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Pomegranate has valuable nutritional content and contains various bioactive compounds, one found in the fruit's peel. The utilization of these bioactive compounds could be used as herbal medicines and supplements, such as antioxidants. This study aimed to determine the antioxidant capacity, phytochemical profile, and pomegranate peel extract grouping using different extracting solvents. The three extracting solvents used were water, 70% ethanol, and ethanol p.a. Antioxidant capacity of the three extracts was measured using the DPPH and CUPRAC methods. We also determined the total phenolic and flavonoid levels and the TLC fingerprint analysis and FTIR spectrum of the pomegranate peel extracts. The 70% ethanol extract owned the largest antioxidant capacity than the other two extracts with a value of 358.67 and 2981.59 Âµmol trolox/g dried sample using the DPPH and CUPRAC methods, respectively. The three pomegranate peel extracts' total phenolic and flavonoid levels ranged from 287.26â€“1068.81 mg GAE/g dried sample and 0.24-0.75 mg QE/g dried sample. TLC fingerprint analysis of pomegranate peel extract yielded 2, 6, and 6 bands for water extract, 70% ethanol, and p.a ethanol, respectively. The three extracts can be grouped based on FTIR spectrum data using principal component analysis using three principal components with a total variance of 93%. The results obtained show that using different extracting solvents provides different antioxidant capacities and phytochemical profiles.

POTENSI SPEKTROSKOPI FTIR-ATR DAN KEMOMETRIK UNTUK MEMBEDAKAN RAMBUT BABI, KAMBING, DAN SAPI Rafi, Mohamad; Anggundari, Widia Citra; Irawadi, Tun Tedja
Indonesian Journal of Chemical Science Vol 5 No 3 (2016)
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/ijcs.v5i3.10654

Rambut beberapa hewan seperti babi, kambing, dan juga sapi telah digunakan sebagai bahan baku kuas salah satunya kuas untuk produksi makanan seperti kue, roti, dan lainnya. Jika kuas dalam produksi makanan ini terbuat dari rambut babi maka dapat menyebabkan makanan menjadi tidak halal. Oleh karena itu, dalam penelitian ini kami melakukan studi keterlaksanaan penggunaan spektroskopi Fourier transform infrared-attenuated total reflectance (FTIR-ATR) yang dikombinasikan dengan kemometrik untuk mengembangkan metode identifikasi dan diskriminasi rambut babi, kambing, dan sapi. Spektrum FTIR diukur pada bilangan gelombang 1000-4000 cm-1. Intensitas dari kisaran bilangan gelombang 1215-2007 cm-1 dan 3467-3989 cm-1 dipilih untuk membuat model diskriminasi tiga jenis rambut yang digunakan. Pengelompokan sampel berdasarkan jenis rambutnya dilakukan dengan menggunakan analisis gerombol, analisis komponen utama, dan analisis diskriminan. Model diskriminasi menggunakan AKU dan AD dapat memisahkan ketiga jenis rambut hewan yang digunakan dengan AD memberikan pengelompokan yang lebih terpisah satu sama lainnya. Metode kombinasi FTIR-ATR dan kemometrik dimungkinkan untuk digunakan untuk tujuan identifikasi dan diskriminasi rambut babi, kambing dan sapi.

Potensi Daerah Sidik Jari Spektrum Inframerah Sebagai Penanda Bioaktovitas Ekstrak Tanaman Obat Latifah Kosim Darusman; Rudi Heryanto; Mohamad Rafi; Wulan Tri Wahyuni
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 3 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.806 KB)

Efficacy and quality of medicinal plant extracts depend on chemical composition therein. Therefore, to ensure its efficacy orquality, the chemical composition of extracts have to be analyzed based on its chemical marker or its chemical pattern. This study tried to develop a method for extracts quality assay based on infrared spectrum in fingerprint region and extracts inhibition activity to xanthine oxidase. Five extracts from kunyit (Curcuma domestica), temulawak ( C xanthorriza), jahe (Zingiber officina/e), temukunci (Boesenbergia pandurata), and cabe jawa (Piper retrofractum) were tested to inhibit xanthine oxidase activity. The bioactive data of extracts and its infrared spectrum was analyzed using PCA and PLS-DA (partial least squarediscriminant analysis). Almost all extracts that were tested showed inhibition activity to xanthine oxidase activity, except the extract of jahe. The result showed that PCA can group all of extract into each of their region. Meanwhile, PLS-DA has shown to be a good prediction model for classifying extracts based on fingerprint region of its mR Spectrum and its biological activity. Keywords: fingerprint, mR spectra, compound marker, xanthine oxidase

Produktivitas Amino Acid Fish Aggregation pada Alat Tangkap Bagan Apung di Perairan Palabuhanratu, Sukabumi Roza Yusfiandayani; Bambang Riyanto; Mohamad Rafi; . Heriyanto
Jurnal Ilmu Pertanian Indonesia Vol. 24 No. 2 (2019): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Fish has two chemical sensory/receptor systems that smell (olfactory) and taste (gustatory) odor that adapt to the specific substance of the environment. In general, olfactory is similar to nasal organs for human nostrils. The olfactory receptors detect chemical stimuli in the form of electrical signals. Based on the ability of fish that can respond to chemical stimuli through the sense of smell, a substance can be used to attract the attention of fish. In response to this reality, AFA (Amino Acid Fish Aggregation) can be developed as a fish collecting tool to attract fish bu using amino acid preparation that works though the sense of smell and taste of fish. The objectives of this study were to design and produce a prototype of Amino Acid Fish Aggregation (AFA), to determine the productivity of catches of raft lift net by using AFA, and to calculate the length of the weight of the catch using AFA. The data used were catch data with weights and lengths of fish catch by raft lift net using AFA. The results showed that the AFA prototype which had a size of p x l x t : 35 x 22 x 15 cm and the highest catches was found at 18.00-00.00 WIB by using AFA with raft lift net. The catches using AFA in Palabuhanratu waters amounted to 5 types of fish, namely stolephorus sp., sardinella sp., rastrelliger kanagurta, euthynnus affinis, trichiurus sp., and positive allometrics were found in rastrelliger kanagurta and anchovy, but negative allometrics were found in euthynnus affinis, sardinella sp., and trichiurus sp.

MODEL OTENTIKASI KOMPOSISI OBAT BAHAN ALAM BERDASARKAN SPEKTRA INFRAMERAH DAN KOMPONEN UTAMA STUDI KASUS : OBAT BAHAN ALAM/FITOFARMAKA PENURUN TEKANAN DARAH Agus Mohamad Soleh; Latifah K. Darusman; Mohamad Rafi
FORUM STATISTIKA DAN KOMPUTASI Vol. 13 No. 1 (2008)
Publisher : FORUM STATISTIKA DAN KOMPUTASI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (97.41 KB)

Komposisi kimia yang terkandung dalam ekstrak obat bahan alam merupakan suatu komposisi yang kompleks, dengan demikian pengujian keotentikannya tidak dapat dilakukan melalui pedekatan tunggal. Salah satu teknik analisis yang dapat menggambarkan secara menyeluruh karakteristik kimia suatu bahan adalah teknik spektroskopi FTIR. Spektra FTIR dihasilkan dari interaksi antara energi sinar inframerah dan komponen kimia penyusun campuran bahan, sehingga suatu spektra FTIR merupakan indentitas khas campuran tersebut. Keotentikan komposisi suatu obat bahan alam pada studi ini ditentukan berdasarkan pada analisis komponen utama spektra inframerahnya. Studi dilakukan pada obat bahan alam/fitofarmaka penurun tekanan darah (Tensigard® : terdiri dari ekstrak seledri dan ekstrak daun kumis kucing). Pengukuran spektra inframerah dilakukan terhadap formula obat yang persentase komposisinya ditentukan melalui simplex lattice design. Selain itu pengukuran spektra inframerah juga dilakukan terhadap formula obat dengan mengganti (adulterasi) ekstrak kumis kucing dengan obat sintetis (reserpin) dan ekstrak sambiloto. Berdasarkan plot antara skor komponen utama pertama dan skor komponen utama kedua menunjukkan plot tersebut dapat digunakan untuk mendeteksi komposisi obat, tetapi tidak dapat mendeteksi adanya adulterasi komposisi oleh bahan lain. Kata Kunci : model otentikasi fitofarmaka, simplex lattice design, komponen utama, tensigard

Metabolite Profiling of Tabat Barito (Ficus deltoidea) Using UPLC-QTOF-MS/MS Nurmaida Nurmaida; Latifah K Darusman; Mohamad Rafi; Rudi Heryanto
The Journal of Pure and Applied Chemistry Research Vol 7, No 2 (2018): Edition May-August 2018
Publisher : Chemistry Department, The University of Brawijaya

Tabat Barito (Ficus deltoidea) is known as a plant that has potency as an antioxidant because of containing a significant phenolic compound. In this study, we performed metabolite profiling on F. deltoidea leaves by UPLC-QTOF-MS/MS to identify its phenolic compounds. Extraction by maceration and ultrasonication techniques with methanol (MM and UM) and ethanol (ME and UE) were used to extract the F. deltoidea metabolites. About 70 metabolites were identified by using UPLC-QTOF-MS/MS in negative ion mode. The amounts of metabolites found in each extract were different, i.e., 45 metabolites in MM, 64 metabolites in UM, 42 metabolites in UE and 41 metabolites in ME. MS/MS could further tentatively identify 16 metabolites. The identified compounds belonged to the class of flavonoids and phenolic acid. Also, we conducted an antioxidant activity by using DPPH method on each extract to determine its potency as an antioxidant. The highest antioxidant activity was exhibited by UM extract (IC50 71.93 ppm) may be due to the number of metabolites in UM extract which was higher than the other extract based on the detected metabolites.

L-Histidine-Modified Silica from Rice Husk and Optimization of Adsorption Condition for Extractive Concentration of Pb(II) Nurhajawarsi Nurhajawarsi; Mohamad Rafi; Utami Dyah Syafitri; Eti Rohaeti
The Journal of Pure and Applied Chemistry Research Vol 7, No 2 (2018): Edition May-August 2018
Publisher : Chemistry Department, The University of Brawijaya

A new chelating agent, L-histidine-modified silica from rice husk (LHSRH), was prepared to increase the adsorption capacity and selectivity for Pb(II). LHSRH was synthesized by immobilizing L-histidine on silica from rice husk (RH) modified with 3-aminopropyltrimethoxysilane (APTMS). Silica from rice husk (SRH) was synthesized via precipitation process by adding hydrochloric acid solution to rice husk ash (RHA). The RHA was subsequently destructed with sodium hydroxide and heated to obtain sodium silicate (Na2SiO3). SRH was characterized by Fourier transform infrared spectroscopy and x-ray diffraction. The LHSRH was used further to adsorp Pb(II) metal ion. The pH range, amount of adsorbent, and adsorption time were optimized by response surface methodology. The optimum condition for the adsorption of Pb(II) was pH 5, an amount of adsorbent 0.1 g; and adsorption time 15 minutes. The adsorption capacity for Pb(II) ion was found to be 62.5 mg/g. The adsorption behavior of the matrix followed the Langmuir’s model.

A Simple Photometer and Chemometrics Analysis for Quality Control of Sambiloto (Andrographis paniculata) Raw Material Rudi Heryanto; Derry Permana; Aryo Tedjo; Eti Rohaeti; Mohamad Rafi; Latifah Kosim Darusman
The Journal of Pure and Applied Chemistry Research Vol 6, No 3 (2017): Edition of September - December 2017
Publisher : Chemistry Department, The University of Brawijaya

In this paper, we described the use of a light emitting diode (LED)-based photometer and chemometric analysis for quality control of king of bitter or sambiloto (Andrographis paniculata) raw material. The quality of medicinal plants is determined by their chemical composition. The quantities of chemical components in medicinal plants can be assessed using spectroscopic technique. We used an “in house” photometer to generate spectra of sambiloto. The spectra were analyzed by chemometric methods, i.e. principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA), with the aim of herbal quality classification based on the harvesting time. From the results obtained, based on thin layer chromatography analysis, sambiloto with different collection times (1, 2, and 3 months) contained different amounts of active compounds. Evaluation of sambiloto, using its spectra and chemometric analysis has successfully differentiated its quality based on harvesting time. PCA with the first two PC’s (PC-1 = 60% and PC-2 = 35%) was able to differentiate according to the harvesting time of sambiloto. Three models were obtained by PLS-DA and could be used to predict unknown sample of sambiloto according to the harvesting time

Identifikasi dan Autentikasi Jahe Merah Menggunakan Kombinasi Spektroskopi FTIR dan Kemometrik Edy Djauhari Purwakusumah; Mohamad Rafi; Utami Dyah Safitri; Waras Nurcholis; Muhammad Agung Zaim Adzkiya
agriTECH Vol 34, No 1 (2014)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

FTIR spectroscopy combined with principal component analysis and discriminant analysis were used for identification and authentication of jahe merah from its related species namely jahe emprit and jahe gajah. FTIR spectra of all samples were recorded in the wavenumber range 4000-400 cm-1 and then subjected for preprocessing signal such as normalization and baseline correction. Combination of FTIR spectra with some chemometrics method such as principal component analysis and discriminant analysis were used to distinguish the three varieties of ginger. By using discriminant analysis, the three varieties of ginger were classified according to its variety. The developed method could be used for identification and authentication of jahe merah.ABSTRAKSpektroskopi FTIR dan kombinasinya dengan analisis komponen utama dan analisis diskriminan telah digunakan untuk identifikasi dan autentikasi jahe merah dari dua spesies yang berkerabat dengannya yaitu jahe emprit dan jahe gajah. Seluruh contoh dibuat spektrum FTIR pada kisaran bilangan gelombang 4000-400 cm-1 dan diberi perlakuan pendahuluan seperti normalisasi dan koreksi garis dasar. Kombinasi spektrum FTIR dengan beberapa metode kemometrik seperti analisis komponen utama dan analisis diskriminan digunakan untuk membedakan ketiga varietas jahe. Analisis diskriminan dapat mengelompokkan ketiga varietas jahe sesuai dengan jenis varietasnya. Metode yang dikembangkan ini dapat digunakan untuk tujuan identifikasi dan autentikasi jahe merah.

PREDIKSI KADAR FLAVONOID TOTAL TEMPUYUNG (Sonchus arvensis L.) MENGGUNAKAN KOMBINASI SPEKTROSKOPI IR DENGAN REGRESI KUADRAT TERKECIL PARSIAL E. Rohaeti; R. Heryanto; M. Rafi; A. Wahyuningrum; L. K. Darusman
Jurnal Kimia (Journal of Chemistry) Vol. 5, No. 2 Juli 2011
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.541 KB)

The potency of Fourier transformed infrared spectroscopy (FTIR) combined with chemometric method for determining the total flavonoid content of tempuyung (Sonchus arvensis L) has been investigated. Partial least squares regression (PLSR) was used to build a prediction model based on the relationship between concentration of total flavonoids obtained from the reference method (AlCl3) and FTIR spectrum. Application of PLSR in the determination of total flavonoids content in tempuyung gave moderate value for goodness of fit of the model with r calibration = 0974, r validation = 0742, Standard Error Calibration = 0023, Root Mean Square Error Calibration = 0023, Standard Error Prediction = 0078, Root Mean Square Prediction Error = 0076, and bias = -0001 using FTIR derivative spectrum segment 1. From these results, combination of FTIR spectrum and PLSR can be used for the prediction of total flavonoids content in tempuyung.

Co-Authors A. Wahyuningrum Abdul Rohman Abdul Rohman Abdul Waris Adi Novriansyah Agus Jatnika Effendi Agus Mohamad Soleh Alfi Hudatul Karomah Ali Miftahudin Allen Kurniawan Amalia Yunita Amrullah Amrullah Antonio Kautsar Aryo Tedjo Asefin Nurul Ikhtiarini Atang Sutandi Auliatifani, Reza Auliya Ilmiawati Baba Barus Bambang - Riyanto Bambang Prajogo Eko Wardoyo Cahya Septyanti Cecep Abdurohman Putra Derry Permana Dewi Anggraeni Septaningsih Dewi Pusparani Sinambela Dien Atin Boritnaban Djoko Santosa Dyan Fitri Nugraha E. Rohaeti Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Edy Djauhari Purwakusumah Esti Mumpuni Eti Rohaeti Eti Rohaeti Eti Rohaeti Fadila Karunina Fifi Gus Dwiyanti Hanifullah Habibie HENNY PURWANINGSIH Herdiyanto Herdiyanto Heriyanto Syafutra Ikrar M. Saleh Indah Mutiara Ningtyas Razaad Irma Herawati Suparto Irmanida Batubara Irnawati Irnawati ISKANDAR ZULKARNAEN SIREGAR Khaydanur, Khaydanur Khoirotul Muzayanah L. K. Darusman Latifah K Darusman Latifah K Darusman Latifah K. Darusman Latifah K. Darusman Latifah Kosim Darusman Latifah Kosim Darusman Latifah Kosim Darusman Latifah Kosim Darusman Lee Wah Lim Liliek Nurhidayati Lisa Aprilia Indriyani Lusi Royani M. Agung Zaim Adzkiya Maeda Wahyuningrum Makmum Syafi’i Maya Melati Muhamad Insanu Muhamad Noor Azizu Muhammad Bachri Amran Muhammad Fuad Zaini Muhammad Majiidu Nancy Dewi Yuliana Nanik Siti Aminah Neliwati Nengsih, Nunuk Kurniati Nita Hestiyana Nurameylia Rasaidi Nurhajawarsi Nurhajawarsi Nurmaida Nurmaida Nursifa Mawadah Partomuan Simanjuntak Parwito Parwito Purnomo, Tsania Firqin Putra Astaman Putri Vidiasari Darsono R. Heryanto Rifki Husnul Khuluk Rita K Sari Rizka Wildani Rizky Kurniawan Murtiyanto Rizna Azrianiningsari Roza Linda Roza Linda Roza Yusfiandayani Rudi Heryanto SANDRA ARIFIN AZIZ Septaningsih, Dewi Anggraini Setyaningsih, Widiastuti Shinta Maharani Trivena Sifa Awalul Fikriah Siti Norazian Ismail Slamet Ari Dwi Ratnanto Suminar Setiati Achmadi Suyanti Natalia Syamsudin Abdillah Syamsudin Abdillah Syifa Muflihah Tanti Yulianti Raga Pertiwi Taopik Ridwan Tatik Raisawati Toyohide Takeuchi Tun Tedja Irawadi Umi Khabibah Usman Ahmad Utami Dyah Safitri Utami Dyah Syafitri Waras Nurcholis Widia Citra Anggundari, Widia Citra Wina Yulianti Wisnu Ananta Kusuma Wulan Tri Wahyuni Wulansari, Laela Yanuar Chandra Wirasembada Yashinta Fitriani Yulita Suyatmika Zulhan Arief Zulhelmi Aziz Zulliati Zulliati

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