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IDENTIFICATION OF SECRETORY STRUCTURE, HISTOCHEMISTRY AND PHYTOCHEMICAL COMPOUNDS OF MEDICINAL PLANT Hyptis capitata Jacq. Darius Rupa; Yohana Caecilia Sulistyaningsih; Dorly Dorly; Diah Ratnadewi
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 24 No. 2 (2017)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2017.24.2.499

Abstract

Hyptis capitata Jacq. (common name: Knobweed or False Ironwort) belongs to Lamiaceae family and is among known traditional medicine. The Anak Dalam Tribe of Jambi Province uses the leaves of H. capitata to cure external and internal wounds. This study was aimed at identifying and analyzing secretory structure, histochemistry and phytochemical content of H. capitata leaves.  The results showed that H. capitata leaves have secretory structures in the form of peltate, capitate and uniseriate glandular trichomes on the upper and lower leaf surfaces, with idioblast cells scattered throughout the leaf mesophyll. Histochemical tests indicated that the peltate trichomes have four head cells, containing alkaloids and terpenoids. This study classified capitate trichomes into two types: 1. type I has one stalk cell and two head cells, all containing  alkaloids, terpenoids and lipophilic compounds; 2. type II has long stalk cells consisting of seven to ten cells with one head cell, all containing alkaloids and terpenoids. Uniseriate glandular trichomes of H. capitata leaves consist of four to eight cells containing alkaloids and terpenoids. The idioblast cells are round-shaped and contain lipophilic compounds. GC-MS analysis showed that H. capitata leaves  contain terpenoid compounds assumed to serve as anti-infective agents, including l-limonene, eugenol, farnesol isomers A, d-nerolidol, hexahydrofarnesol and neophytadiene.
Essential Oil Components, Metabolite Profiles, and Idioblast Cell Densities in Galangal (Kaempferia galanga L.) at Different Agroecology Subaryanti Subaryanti; Yohana Caecilia Sulistyaningsih; Dyah Iswantini; Triadiati Triadiati
AGRIVITA, Journal of Agricultural Science Vol 43, No 2 (2021)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v43i2.2631

Abstract

Galangal is widely cultivated for the multifunctional plant. This study analyzed the essential oil chemical components, metabolite profiles, and idioblast cell densities in seven galangal accession grown at two different altitudes (locations A and B). The galangal accessions included: Purbalingga, Cilacap, Purworejo, Karanganyar, Pacitan, Madiun, and Galesia 2 which was used as the control. The results showed that the highest essential oil content was obtained from MAD (3.22%) at location A. The highest levels of ethyl-p-methoxycinnamate (EPMC) were obtained from PBG (74.8%) at location B and PCT (71.6%) at location A. The metabolite profiles of the galangal rhizomes were divided into two clusters based on the metabolite content. The first cluster had one accession: PCT from location A with genkwanin as the metabolite marker. The second cluster consisted of CLP from location A and PBG, MAD, and GAL2 from location B. The highest density of idioblast cells was found in PCT (90.5 cells/mm2) at location A and PBG accessions (77.1 cells/mm2) at location B. The PBG and PCT accessions can be recommended as a candidate of superior varieties based on their high EPMC content.
Localization of Alkaloid and Other Secondary Metabolites in Cinchona ledgeriana Moens: Anatomical and Histochemical Studies on Fresh Tissues and Cultured Cells Dian Rahma Pratiwi; Yohana Caecilia Sulistyaningsih; Diah Ratnadewi
HAYATI Journal of Biosciences Vol. 27 No. 1 (2020): January 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (544.992 KB) | DOI: 10.4308/hjb.27.1.1

Abstract

Cinchona ledgeriana produces several secondary metabolites. The main quinoline alkaloid, quinine that is widely used as an antimalarial drug, is most commonly extracted from the bark of Cinchona, and its leaves contain several other metabolites. Many studies have revealed that cell culture of Cinchona also produces quinine. Nevertheless, the sites of secondary metabolites accumulation are still elusive. This study is aimed at describing specific anatomical structures where alkaloids and some other secondary metabolites are accumulated as well as their localization in leaves and barks of C. ledgeriana, compared to those found in cultured cells. Fresh leaves and barks, and cells of C. ledgeriana were used for anatomical observation and histochemical tests. It was found that these plant parts have specialized structures, idioblast cells with elliptical- and spherical-shapes, scattered in leaf hypodermis, stem cortex, and secondary phloem. Unspecialized structures such as epidermis and palisade mesophyll tissues were also found accumulating some metabolites. Histochemical tests showed that bark and leaves contained alkaloids, terpenoids, phenolic, and lipophilic compounds. Cultured cells presented positive results for alkaloids and terpenoids.
Pertumbuhan dan Produksi Rimpang Kencur (Kaempferia galanga L.) pada Ketinggian Tempat yang Berbeda Subaryanti Subaryanti; Yohana Caecilia Sulistyaningsih; Dyah Iswantini; Triadiati Triadiati
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 2 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (428.012 KB) | DOI: 10.18343/jipi.25.2.167

Abstract

Galanga (Kaempferia galanga L.) is one of the potential medicinal plants with high demand. Therefore, galanga cultivation was still quite promising. To obtain the optimum growth and high rhizome production, the superior galanga accessions are required. The optimal growth and high production of galanga accessions will be obtained if cultivated at the area with appropriate altitude. The purpose of the study was to obtain galanga accession with a better growth and high yield at the area with a suitable altitude. This study used split-plot design; altitude as a main plot and accession as a subplot. The location as a main plot consisted of altitudes of 214 and 780 masl. This study used seven accessions i.e., accession of PBG (Purbalingga), CLP (Cilacap), PWJ (Purworejo), KRA (Karanganyar), PCT (Pacitan), MAD (Madiun), and GAL2 (Galesia 2) as control. The results showed that different altitudes significantly affected the total chlorophyll content, stomatal conductance, transpiration rate, photosynthesis rate, dry weight, and rhizome yield. The number of leaves was influenced by altitude and accession of galanga. The interaction between altitude and accession of galanga significantly affected the leaf area and the soluble sugar concentration in the galanga rhizome. The rhizome yield at low altitude was higher than in high altitude. PBG (Purbalingga) and PWJ (Purworejo) accessions have the potential to be developed in locations with an altitude of 214 masl. Keywords: altitude, Kaempferia galanga, rhizome
Analisis Morfofisiologi, Anatomi, dan Histokimia pada Lima Spesies Tanaman Gulma sebagai Respons terhadap Merkuri dan Timbal Rani Apriyani Raharja; Hamim Hamim; Yohana Caecilia Sulistyaningsih; Triadiati Triadiati
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 3 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.25.3.412

Abstract

Plants can be used as phytoremediation agents to improve critical land due to gold mining activities. This experiment aimed to analyze the morphophysiological, anatomical, and histochemical responses of Brachiaria mutica, Cyperus kyllingia, Ipomea aquatica, Mikania micrantha, and Paspalum conjugatum in response to the application of heavy metals mercury and lead in the forms of Hg(NO3)2 and Pb(NO3)2 in hydroponic experiments and to determine the ability of these plants to accumulate both metals. Morphological responses were observed by measuring number of leaves and plant dry weight, and physiological responses were observed by analyzing photosynthetic pigments, while anatomical and histochemical responses were analyzed by microscopic observation to tranversal slice of roots and leaves. The results showed that the applications of Hg(NO3)2 and Pb(NO3)2 treatments caused decreases in number of leaves, plant dry weights, and photosynthetic pigments (chlorophyll a, chlorophyll b, and carotenoid). The treatments also decreased leaf thickness due to the decrease in the epidermis, but they caused the increases in exodermis and endodermis of the roots. Mercury and lead were accumulated in large amounts in the roots, but accumulation in the shoot was less. Histochemical observation showed that lead was found in the roots of all the plants, especially in endodermic tissue and the vessel, whereas in the leaves the two metals were detected in the upper and lower epidermis, mesophyll, and vessel. Among the five species tested, C. kyllingia and P. conjugatum were the most tolerant to Pb and Hg.
Struktur Sekretori Aglaonema simplex sebagai Tumbuhan Obat Evi Muliyah; Yohana Caecilia Sulistyaningsih
SIMBIOSA Vol 11, No 1 (2022): JURNAL SIMBIOSA
Publisher : Universitas Riau Kepulauan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33373/sim-bio.v11i1.3916

Abstract

Aglaonema simplex (Selimpot pati) merupakan suku Araceae yang digunakan untuk pengobatan tradisional. Batang dan akar tumbuhan A. simplex dimanfaatkan oleh Suku Anak Dalam di Provinsi Jambi, Indonesia, untuk mengobati gejala diare. Penelitian ini bertujuan untuk mengidentifikasi struktur sekretori batang dan akar A. simplex. Studi histologi struktur sekretori batang dan akar A. simplex telah dilakukan. Spesies ini memiliki struktur sekretori berupa sel idioblas. Sel idioblas pada akar tersebar di korteks. Pada batang, sel idioblasnya tersebar pada epidermis, korteks, dan empulur. Sel idioblas pada A. simplex memiliki bentuk silindris. Ukuran sel idioblas terbesar ditemukan pada bagian empulur batang. Kerapatan sel idioblas tertinggi ditemukan pada epidermis batang.