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Loekas Soesanto
Universitas Jenderal Soedirman
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Keagresifan Beberapa Isolat Fusarium oxysporum f. sp. zingiberi Asal Temanggung dan Boyolali Setelah Penyimpanan dalam Tanah Steril Henky Setyawan Norandika Wahyu; Loekas Soesanto; Kustantinah Kustantinah
Jurnal Fitopatologi Indonesia Vol 8 No 6 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.248 KB) | DOI: 10.14692/jfi.8.6.170

Abstract

Preservation of fungi isolates is needed for further research but usually the preservation treatment is only concerned on life span and growth of the fungi. This research aimed at knowing life span and aggressivity of Fusarium oxysporum f. sp. zingiberi isolates from ginger cv. Gajah after preserving for three years in sterile soil. Completely Randomized Design and Randomized Block Design were used for in vitro and in planta test, respectively, with 10 isolates of F. oxysporum f. sp. zingiberi from Temanggung, 8 isolates of F. oxysporum f. sp. zingiberi from Boyolali, and 1 control, each repeated three times. Variables observed were incubation period, infected area, disease intensity, rhizome wet weight, number of leaves, and crop height. Result of the research showed that all isolates of F. oxysporum f. sp. zingiberi could still grew well. Based on origin of the isolates, the highest aggressivity on ginger rhizome was TKO3 from Temanggung and BAO6 from Boyolali with infected area of 67.67 mm2 and 56.67 mm2, respectively. On ginger crop, the highest aggressivity was BAO1 from Boyolali and TPO2 from Temanggung with disease intensity of 68.09% and 38.13%, respectively. All isolates of F. oxysporum f. sp. zingiberi did not cause significantly different effect on incubation period, disease intensity, crop height, leaves total, and rhizome wet weight but they caused significantly different effect on infected area.   Key word: aggressivity, Fusarium oxysporum, ginger, preservation, steril soil
Aplikasi Formula Cair Pseudomonas fluorescens P60 untuk Menekan Penyakit Virus Cabai Merah Loekas Soesanto; Endang Mugiastuti; Ruth Feti Rahayuniati
Jurnal Fitopatologi Indonesia Vol 9 No 6 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (305.71 KB) | DOI: 10.14692/jfi.9.6.179

Abstract

Viral diseases of chilli pepper are difficult to control, therefore the use of Pseudomonas fluorescens P60 should be evaluated. The aims of this research were to determine the influence of liquid formula of P. fluorescens P60 on virod disease and on growth and yield of chili pepper. Randomized block design (RBD) experiment was composed of 7 treatments and 4 replicates, i.e., control, insecticide applicaton, P. fluorescens P60 application by seedling drenching and spraying for 1, 2, 3, 5, and 7 times. The result showed that 5 times application of P. fluorescens P60 by drenching and spraying was able to suppress viral disease and reduce disease intensity by to 73.37%, increasing density level of P. fluorescens P60 to 9.50 x 1011 and increase phenolic compounds (saponin, tannin and glycoside). The same treatment could increase plant height 23.7%, root lenght 6.44%, plant dry weight 66.68%, root dry weight 23.59%, and yield weight 53.16%.
Kemampuan Campuran Bacillus sp., Pseudomonas fluorescens, dan Trichoderma sp. untuk Mengendalikan Penyakit Layu Bakteri pada Tanaman Tomat abdul manan; Endang Mugiastuti; Loekas Soesanto
Jurnal Fitopatologi Indonesia Vol. 14 No. 2 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (278.158 KB) | DOI: 10.14692/jfi.14.2.63

Abstract

Ability of Bacillus sp., Pseudomonas fluorescens, and Trichoderma sp. to Control Tomato Bacterial Wilt DiseaseRalstonia solanacearum is known as the causal agent of bacterial wilt on tomato.  The bacteria may infect all stadia of plant growth and decrease tomato production. Biological control using antagonistic microbes is considered as a potential control alternative for the disease. This research was aimed to assay the ability of combination treatment of Bacillus sp., Pseudomonas fluorescens, and Trichoderma sp. in controlling R. solanacearum and its effect on growth and yield of tomato in the field. The treatments consisted of control (without antagonistic microbes), mixed combination of Bacillus sp. B8 + Bacillus sp. B11 + Trichoderma sp.; Bacillus sp. B8 + Pseudomonas flourescens P8 + Trichoderma sp.; and Streptomisin sulfat 20%. Antagonistic microbes was applied at planting time, as much as 100 mL per plant. The result showed that mixed combination of Bacillus sp. B8 + Bacillus sp. B11 + Trichoderma sp. was the best treatment in controlling the disease as indicated by delaying incubation period up to 6.2 days, decreasing disease incidence up to 12.3%, increasing plant growth up to 42.80%, and increasing yield up to 14.99%.
Aplikasi Pseudomonas fluorescens P20 formula cair tepung ikan terhadap rebah semai mentimun Hening Kurniasih; Nur Prihatiningsih; Endang Mugiastuti; Loekas Soesanto
Jurnal Agro Vol 7, No 2 (2020)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/8591

Abstract

Rebah semai merupakan penyakit penting bibit mentimun, dan formula cair Pseudomonas fluorescens P20 dengan tepung ikan perlu dicoba untuk mengatasi hal tersebut. Penelitian bertujuan mencari konsentrasi tepat tepung ikan untuk P. fluorescens P20, pengaruhnya dalam menekan rebah semai, dan terhadap pertumbuhan mentimun. Penelitian dilaksanakan di Screen House Fakultas Pertanian Universitas Jenderal Soedirman, Purwokerto selama empat bulan. Rancangan Acak Lengkap digunakan pada in vitro dengan 5 ulangan dan 5 perlakuan terdiri atas kontrol (King’s B cair), serta tepung ikan 10, 20, 30, dan 40 g L-1. Uji in planta menggunakan Rancangan Acak Kelompok dengan 5 ulangan dan 6 perlakuan terdiri atas kontrol, King’s B cair, serta tepung ikan 10, 20, 30, dan 40 g L-1. Variabel yang diamati adalah populasi bakteri, masa inkubasi, kejadian penyakit, area under the disease progress curve (AUDPC), tinggi tanaman, panjang akar, bobot tanaman segar, dan bobot akar segar. Hasil penelitian menunjukkan bahwa P. fluorescens P20 dalam tepung ikan 10 g L-1 memiliki populasi tertinggi yaitu 3,99 x 1021 upk mL-1 atau meningkat sebesar 47,23%. Saat diaplikasikan, formula bakteri ini paling efektif menunda masa inkubasi dan menekan kejadian penyakit hingga 100% dan nilai AUDPC terendah yaitu 0%-hari. Perlakuan mampu meningkatkan panjang akar 5,30-31,19% dan bobot akar segar 10,81-65,85%. Damping-off is an important cucumber seedlings disease, and liquid formulation of Pseudomonas fluorescens P20 is developed to overcome this problem. This research aimed to gain the right concentration of fish flour for P. fluorescens P20, its effect on suppressing damping-off and on cucumber growth. The research was conducted at the Screen House, Faculty of Agriculture, Jenderal Soedirman University, Purwokerto for four months. Randomized completely design was used for in vitro test with 5 treatments consisted of control, fish flour of 10, 20, 30, and 40 g L-1repeated 5 times. Randomized block design was used for in planta test with 6 treatments consisted of control, King’s B Broth, flour liquid of 10, 20, 30, and 40 g L-1repeated 5 times. Variables observed were bacterial population, incubation period, disease incidence, area under the disease progress curve (AUDPC), plants height, roots length plants fresh weight, and roots fresh weight. Result showed that P. fluorescensP20 in fish flour of  10 g L-1 had the highest population as 3.99 x 1021 cfu ml-1 or increase as 47.23%. Application of this formula could delay incubation period and suppress disease incidence as 100% and decrease AUDPC as 0%-days. The formula could increase roots length and roots fresh weight as 5,30-31,19 and 10,81-65,85%.
EKSPLORASI DAN UJI VIRULENSI BAKTERI Bacillus sp. ENDOFIT JAGUNG TERHADAP PENYAKIT BUSUK PELEPAH JAGUNG Arum Saputri; Loekas Soesanto; Abu Umayah; Agus Sarjito
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 22, No 2 (2020)
Publisher : Program Studi Ilmu Tanah Fakultas Pertanian UNIB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.22.2.70-78

Abstract

[EXPLORATION AND VIRULENT TEST OF MAIZE ENDOPHYTE Bacillus sp. AGAINST MAIZE SHEATH BLIGHT]. Capability of endophytic bacterial, effectivity, and its effect on R. solani. and on maize seedlings growth were investigated from April 2018 to January 2019. Exploration of endophytes bacteria in maize was taken from Banyumas Regency (Sumbang, Kembaran, Baturraden) and Purbalingga Regency (Padamara, Bojongsari, Pratin). Taking plant samples using Purposive Random Sampling and Diagonal Sampling methods. Completely randomized design was used in in vitro test with 16 treatments repeated twice. Completely randomized block design was used in in planta experiment with 5 treatments repeated 5 times. The treatment consisted of control, fungiside (mankozeb), and 2 isolates of endophytes bacteria performing the best in vitro result. Variables observed included characteristics of endophytic bacteria and pathogenic fungi, inhibition diameter, incubation period, disease intensity, incidence of disease, AUDPC, plant height, leaf number, root length, plant fresh weight, canopy fresh weight, and root fresh weight. Resultshowed that the exploration obtained 15 endophytic Bacillus sp. isolates. The PD A.4 and BK A.1 isolates werw able to inhibit the growth of pathogenic fungi in-vitro by 56.93 and 51.5%, respectively. The soaking treatment using BK A1 was able to reduce disease intensity by 59.377%, and AUDPC value 34.19%. Endophytic bacteria influence plant height, plant fresh weight, canopy fresh weight, and fresh weight of roots respectively as 89.17 cm, 126.06 g, 106.67 g and 19.4 g.
KEEFEKTIFAN BAKTERI Serratia ENDOSIMBION WBC TERHADAP ULAT GRAYAK (Spodoptera litura F.) DI LABORATORIUM ENTOMOLOGI BBPOPT JATISARI KARAWANG Fina Dwimartina; Rostaman Rostaman; Loekas Soesanto
Agro Wiralodra Vol. 3 No. 1 (2020): Jurnal Agro Wiralodra
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/agrowiralodra.v3i1.39

Abstract

Penelitian ini bertujuan untuk: 1) Mengetahui pengaruh perlakuan bakteri Serratia Endosimbion WBC terhadap mortalitas serangga S. litura; 2) Mengetahui pengaruh pemberian bakteri tersebut terhadap daya konsumsi larva S. litura; 3) Mengetahui pengaruh bakteri tersebut terhadap perkembangan serangga. Penelitian dilaksanakan di Laboratorium Entomologi BBPOPT Jatisari Karawang, pada bulan Maret sampai Juli 2014. Penelitian ini dilakukan dengan metode eksperimen, menggunakan rancangan acak terpisah yang terdiri dari 10 perlakuan dan 2 kali ulangan. Variabel yang diamati adalah mortalitas (%), tingkat konsumsi larva, dan perkembangan larva. Hasil penelitian menunjukkan bakteri Serratia tidak menimbulkan kematian pada serangga uji (mortalitas 0%), tidak berbeda nyata pada daya konsumsi larva, dan tidak berpengaruh nyata terhadap persentasi larva yang menjadi pupa. Bakteri Serratia berpengaruh nyata terhadap berat pupa dan persentasi pupa menjadi imago.
Penerapan Teknologi Pengendalian Penyakit Tanaman Yang Ramah Lingkungan Pada Tembakau Endang Mugiastuti; Loekas Soesanto; Abdul Manan
JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat) VOL. 2 NOMOR 2 SEPTEMBER 2018 JPPM (Jurnal Pengabdian dan Pemberdayaan Masyarakat)
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (908.003 KB) | DOI: 10.30595/jppm.v2i2.1793

Abstract

Tobacco is one of the most important plants which were cultivated by Farmers Groups "Darma Mukti Tani I" and "Karya Tani" of Wangon District, Banyumas Regency. However, several obstacles often interfere with the production of tobacco, including quite high tobacco pests and diseases. Socialization of  plant pests diseases management by using Trichoderma sp. and Pseudomonas fluorescens biopesticides, needs to be done. The activity aimed was to increase knowledge and skills of farmers on pests and diseases in tobacco and their management measures with biological pesticides. Issues addressed farmers partners with several technology solution, which was conducted by the transfer of technology through education, training, demonstration plots, and mentoring. Dissemination activities were able to increase their knowledge of pests and diseases, their control, and the control of environmental friendly as 58,82, 62,5, and 67.67%, respectively. The training activities of P. fluorescens exploration was able to improve their ability as 166.67%. Propagation activity and application training could improve their ability for 100 and 73,33%, respectively. The training activities of Trichoderma sp. exploration were able to improve their ability as 94,44%, while the propagation and application training could improve their ability, respectively, as 73,33 and 88.23%. Application of biological pesticides P. fluorescens and Trichoderma sp. at demonstration plot was able to increase growth and reduced the intensity of bacterial and fusarium wilt disease by 40-50%.Keywords: Tobacco, technology transfer, pests and diseases, biological control.
Pelatihan Teknologi Budidaya dan Pengelolaan Penyakit Ramah Lingkungan Pada Tanaman Lada Endang Mugiastuti; Loekas Soesanto; Abdul Manan
JURNAL PENGABDIAN KEPADA MASYARAKAT Vol 8, No 2 (2018): Desember 2018
Publisher : LPPM UNINUS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.614 KB) | DOI: 10.30999/jpkm.v8i2.279

Abstract

Pepper is an important commodity and has high economic value. This commodity has begun to be cultivated by members of the PKK RT 07 and PKK RT 09, Tambak Sogra Village, Sumbang District, Banyumas Regency, in an effort to increase the use of the yard and increase family income. However, the knowledge and skills of PKK members regarding cultivation and diseases of pepper plants as well as effective and environmentally friendly management efforts are very low. The activity aimed was to improve knowledge and skills of members of the PKK group in the cultivation and management of pepper disease that are effective and environmentally friendly. Activities carried out with dissemination, training, and technology demonstration plots. The activities were able to increase the knowledge and skills of members of the PKK group in pepper cultivation and the management of the disease which is environmentally friendly. In addition, the application of Trichoderma sp. and Pseudomonas fluorescens in the demonstration plot can increase the growth of pepper.
Sosialisasi dan Pelatihan Pemanfaatan Biopestisida Mikroba Antagonis Campuran untuk Mengendalikan Penyakit Bawang Merah di Musim Hujan Abdul Manan; Endang Mugiastuti; Loekas Soesanto
Jurnal Pengabdian Pada Masyarakat Vol 6 No 1 (2021)
Publisher : Universitas Mathla'ul Anwar Banten

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30653/002.202161.630

Abstract

SOCIALIZATION AND TRAINING ON THE UTILIZATION OF MIXED ANTAGONISTIC MICROBIAL BIOPESTICIDES TO CONTROL SHALLOTS IN THE RAINY SEASON. The constraints on shallot cultivation in the rainy season are the more severe attacks of Fusarium oxysporum (Fusarium wilt) and Alternaria porii (purple disease). Solid formula of Trichoderma sp., Bacillus sp. B8, and Bacillus sp. B11 have been proven to increase their effectiveness and also cultivation technique and environmental modification. However, the benefit of the technology did not informed members of Farmers Group in Tambaksogra Village, Sumbang Regency, Banyumas District yet. The objectives of the activity are to increase farmers’ knowledge and skills on shallot cultivation technique out of the season, to multiply and to apply the antagonistic microbes solid mixed formula, to increase the crop production quantity and quality, and to increase farmers’ income. The method used was transfer of the technology through education, training, and demplot. The activities carried out were socialization of the potency and application of shallot cultivation technique out the season, multiplication and application of the antagonistic microbial solid mixed formula with the use of available media at the location, demonstration plot of the shallot cultivation technology with the application of the solid mixed formulation compared to single antagonistic, and farmers’ accompaniment in implementing the technology package. Result showed that there was an increase in farmers’ knowledge on the potency and application of solid formula biopesticides by 69.61%, as well as an increase skill in making solid formula biopesticide and its application by 64.33%.
PENGOMPOSAN LIMBAH SAYUR DENGAN EMPAT ISOLAT Trichoderma harzianum DAN PENGARUHNYA TERHADAP PERTUMBUHAN TANAMAN MENTIMUN IN PLANTA Fida Suci Ersapoetri; Loekas Soesanto; Endang Mugiastuti; Ruth Feti Rahayuniati; Abdul Manan; Slamet Rohadi
Agrin Vol 24, No 2 (2020): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.agrin.2020.24.2.536

Abstract

Penelitian bertujuan mengetahui kemampuan empat isolat Trichoderma harzianum dalam mengomposkan limbah sayur, isolat T. harzianum terbaik pada pengomposan limbah sayur, dan isolat T. harzianum terhadap pertumbuhan tanaman mentimun in planta. Penelitian dilaksanakan di rumah kaca dan Laboratorium Perlindungan Tanaman, Fakultas Pertanian, Universitas Jenderal Soedirman, Purwokerto selama lima bulan. Rancangan Acak Kelompok digunakan dengan empat ulangan dan kombinasi antara empat isolat T. harzianum (T10, T213, T14, dan T15) dengan dua limbah sayur (kubis dan tomat). Variabel yang diamati adalah panjang tanaman, bobot tanaman segar, bobot tanaman kering, panjang akar, jumlah daun, pH akhir kompos, C/N ratio kompos, kepadatan akhir T. harzianum, kegigasan T. harzianum, dan analisis jaringan secara kualitatif. Hasil penelitian menunjukkan bahwa keempat isolat T. harzainum efektif dan cepat dalam mengkomposkan limbah tomat dan kubis. Isolat T. harzianum yang paling baik pada pengomposan adalah T10 dan T213. Aplikasi kompos limbah mampu meningkatkan pertumbuhan tanaman mentimun. Isolat terbaik adalah T. harzianum T16 pada kompos tomat dan T10 pada kompos kubis dalam meningkatkan pertumbuhan tanaman mentimun pada panjang tanaman, panjang akar, bobot tanaman segar, dan bobot tanaman kering dengan peningkatan masing-masing 66,61 dan 52,17%, 61,01 dan 46,55%, 76,41 dan 59,77%, serta 77,99 dan 52,03%.Kata kunci: limbah sayur, mentimun, pengomposan, Trichoderma harzianum.
Co-Authors abdul manan Abdul Manan Abu Umayah Agus Sarjito Agus Suroto Anida Amalia Rahma Arum Saputri Dede Yusup Ziaulhak Dwimartina, Fina Endang Mugiastuti Fida Suci Ersapoetri Hening Kurniasih Henky Setyawan Norandika Wahyu Hidayatul Ilahiyyah Kustantinah Kustantinah Muhamad Bahrudin Ni Wayan Anik L Nur Prihatiningsih Rostaman Rostaman Ruth Feti Rahayuniati Slamet Rohadi Tamad wahyu febriyono