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Karakteristik Morfologi dan Fisiologi Isolat Pantoea stewartii subsp. stewartii pada Jagung Yulfi Desi; Trimurti Habazar; Agustian Agustian; Ujang Khairul; Syamsuwirman Syamsuwirman; Prima Novia
Jurnal Fitopatologi Indonesia Vol 10 No 2 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (562.074 KB) | DOI: 10.14692/jfi.10.2.45

Abstract

Pantoea stewartii subsp. stewartii is the pathogen causing stewart’s wilt disease in maize. The occurrence of the disease was recently reported in Indonesia with limited information. Research was conducted to characterize several isolates of P. stewartii subsp. stewartii isolated from maize plants collected from 6 districts in West Sumatra. Leaves showing yellow stripes symptoms along the vein was ground and suspeneded then the bacteria was isolated and incubated for 3 × 24 h at room temperature in nutrient glucose agar (NGA). Pure bacterial colonies gained from this medium was used for characterization. Assessment of bacterial characters was conducted based on morphological assay, physiological assay, biological assay (hypersensitivity, and pathogenicity) followed by polymerase chain reaction (PCR)-based identification. Unique characters of the family Enterobacteriaceae was observed on 7 isolates based on morphological and physiological characters, and only 4 isolates (PR1, PP, ST1, LA) gave positive reaction on hypersensitivity and pathogenicity test. Further molecular identification confirmed 3 out of 7 isolates had high homology to Pantoea stewartii, i.e. Pantoea stewartii strain R1-104, R1-132, and ATCC 29923 for isolates LA, PR1, and PR2, respectively.
Diversity of Beneficial Insect in Corn Plantation at West Sumatra Martinus Abuzar; Ujang Khairul; Hasmiandy Hamid
Jurnal Perlindungan Tanaman Indonesia Vol 25, No 2 (2021)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.61254

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Corn is one of Indonesia's agricultural products with economical and strategic value and many potential to develop, such as carbohydrates and protein sources. There are two types of beneficial insects in corn production: predators and parasitoids. This study aims to identify the diversity of beneficial insects in corn plantations. This study was conducted from May to September 2019 in four different locations across West Sumatra, namely Padang Pariaman, West Pasaman, Pesisir Selatan, Solok Regencies. Identification of beneficial insects was made in Entomology Laboratory, Faculty of Agriculture, Andalas University. This study adopted a purposive sampling method using 20 sweeps of sweep nets, following a transect line of cornfields. The result showed five orders, 34 families, 101 morphospecies, and 747 beneficial insects in all locations. The diversity in each location varies from high, medium, and low. In all areas, the diversity index was high, with the criteria of ≥ 2.90, implying that the condition of the community structure was very stable. From the evenness index analysis, three locations were classified as high, and the other one was moderate. These three sites were Padang Pariaman, West Pasaman, and Solok, with criteria of ≥ 0.90 and very stable distribution conditions. 
Distribution of Clavibacter michiganensis subsp. michiganensis in Various Tomato Production Centers in Sumatra and Java APRIZAL ZAINAL; ASWALDI ANWAR; UJANG KHAIRUL; . SUDARSONO
Microbiology Indonesia Vol. 2 No. 2 (2008): August 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (197.087 KB) | DOI: 10.5454/mi.2.2.3

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Bacterial canker, caused by Clavibacter michiganensis subsp. michiganensis is a newly introduced disease of tomatoes in Indonesia. Its existence was first officially reported in 2004. The objective of this research was to monitor the existence of C. michiganensis subsp. michiganensis in various tomato production centers in Sumatra and Java. Tomato samples showing symptoms of C. michiganensis subsp. michiganensis infection were collected from various tomato production centers in Sumatera and Java and the causal agents were isolated from these samples. Based on the occurrences of typical symptoms of C. michiganensis subsp. michiganensis infection in tomato, the incidence of suspected C. michiganensis subsp. Michiganensis infection ranged from 1-20%. From a total of 74 tomato plants sampled, 24 bacterial isolates exhibiting similar colony morphology to C. michiganensis subsp. michiganensis were obtained. After various physiological, hypersensitive response, and pathogenicity tests, 18 isolates derived from 14 tomato production centers in eight provinces in Sumatera and Java were identified as C. michiganensis subsp. michiganensis. Although the incidence was still low, results of these experiments further indicated that C. michiganensis subsp. michiganensis existed in Indonesia and had spread in a number of tomato production centers in Sumatra and Java. Positive results of C. michiganensis subsp. Michiganensis identification from suspected tomato samples should be a warning sign for all tomato production stakeholders in Indonesia, especially those responsible in regulating the seed trade, importation, and plant quarantine
PEMANFAATAN TEKNOLOGI PENGELOLAAN OPT TANAMAN SAYURAN BERBAHAN BAKU RAMAH LINGKUNGAN DI KANAGARIAN LASI KECAMATAN CANDUANG KABUPATEN AGAM Ujang Khairul; Arneti Arneti; Reflin Reflin
Jurnal Hilirisasi IPTEKS Vol 1 No 4.a (2018)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (657.467 KB)

Abstract

Kegiatan tentang pemanfaatan teknologi pengelolaan organisme penganggu tanaman (OPT) pada tanaman sayuran berbahan baku ramah lingkungan telah dilakukan di Kelompok Tani Mitra di Nagari Lasi Kecamatan Canduang Kabupaten Agam pada bulan September hingga November 2016. Kegiatan ini bertujuan untuk meningkatkan pengetahuan petani tentang serangan OPT sayuran dan bagaimana mengelola OPT menggunakan biopestisida dan pestisida botani. Kegiatan ini meliputi: pemantauan tingkat serangan OPT di lahan mitra, penyuluhan, pelatihan, dan evaluasi hasil kegiatan. Hasil pemantauan tingkat serangan OPT pada tanaman sayuran mitra berkisar antara 18 - 27% untuk penyakit dan 15 - 20% untuk hama. Dari kegiatan dapat dikesimpulkaan yaitu: (1) tingkat OPT pada tanaman sayuran mitra cukup tinggi, (2) Petani belum memahami OPT yang menyerang tanaman sayuran mereka, (3) Petani tidak memahami metode pengelolaan OPT menggunakan biopestisida dan pestisida botani, dan (4) penyuluhan dan praktek lapangan, telah meningkatkan pengetahuan petani tentang pengelolaan OPT sayuran dengan menggunakan biopestisida dan pestisida botani.
PEMBERDAYAAN KELOMPOK TANI MELALUI PELATIHAN PENGENDALIAN HAMA DAN PENYAKIT TANAMAN KAKAO DI KANAGARIAN CAMPAGO KABUPATEN PADANG PARIAMAN Ujang Khairul; Trizelia Trizelia; Reflin Reflin
BULETIN ILMIAH NAGARI MEMBANGUN Vol 1 No 4 (2018)
Publisher : LPPM (Institute for Research and Community Services) Universitas Andalas Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (502.471 KB) | DOI: 10.25077/bina.v1i4.41

Abstract

Training on methods of pests and diseases management in cocoa plants has been conducted in the Nagari Campago, Kecamatan V Koto Kampung Padang Pariaman. The aims of this activity was to empower farmer groups through increasing the knowledge of cocoa farmers about cultivation and pests and diseases management attacked of cocoa plant, this activity consists of socialization, counseling and field practice. Evaluations carried out before and after training related to farmers' knowledge about cultivation and pests and diseases in cocoa. The results of the activity show that: (a) the level of pests and diseases in cocoa plantations in this area is quite high (55% dan 65%), (b) farmers have not good cultivated on cocoa, (c) farmers do not understand abaut pests and diseases that attack their cocoa and do not understand the methods for controlling pests and diseases. This activity has increased farmers' knowledge about the cultivation and management of pests and diseases in cocoa plants.
Pemanfaatan Teknologi Pengelolaan OPT Tanaman Sayuran Berbahan Baku Ramah Lingkungan Di Kanagarian Paninjauan Kabupaten Tanah Datar Ujang Khairul; Reflin Reflin; Arneti Arneti
Warta Pengabdian Andalas Vol 23 No 1 (2016): Warta Pengabdian Andalas
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.23.1.11.2016

Abstract

Activity of use management technologogy for pest and plant disease control on vegetables based environment friendly in nagari Paninjauan Kabupaten Tanah Datar has been carried out from June to November 2014. This activity aims to improve farmers knowledge about the diseases attack of vegetables and know how to manage the disease using biopesticide and botanic biopesticide, These activities include: Counseling and training, and evaluation. Results of monitoring the level of disease in vegetable crops is known that those plants attacked by pest and pathogens e.i, soft rot (cabbage), anthracnosa, bacterial wilt and leaf spot (chili), tuber rot ( carrots) and bacterial leaf blight and Alternaria spots (onion leaves). Attack rate ranged between 16 – 20% for disease and 15 – 30% for pest. From the activities can be take several conclusions: (1) Extent of disease in vegetable crops in this area is high, (2) The farmers do not yet understand the causes of pest and plant disease (pathogens) that attack their vegetable crops, (3) The farmers do not understand the methods of disease control using biopesticide and botanic biopesticide, (4) The extension and field practice, have improved farmers' knowledge about the management of vegetable diseases using biopesticides and botanic biopesticide
Pemanfaatan Teknologi Pengelolaan OPT Tanaman Sayuran Berbahan Baku Ramah Lingkungan di Kanagarian Candunang Kab Agam Ujang Khairul; Arneti Arneti; Reflin Reflin
Warta Pengabdian Andalas Vol 23 No 1 (2016): Warta Pengabdian Andalas
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Activity of use management technologogy for pest and plant disease control on vegetables based environment friendly in nagariPaninjauan Kabupaten Tanah Datar has been carried out from June toNovember 2014. This activity aims to improve farmers knowledgeabout the diseases attack of vegetables and know how to manage thedisease using biopesticide and botanic biopesticide, These activities include: Counseling and training, andevaluation. Results of monitoring the level of disease in vegetable cropsis known that those plants attacked by pest and pathogens e.i, soft rot (cabbage), anthracnosa, bacterial wilt and leaf spot (chili), tuber rot (carrots) and bacterial leaf blight and Alternaria spots (onion leaves).Attack rate ranged between 16 – 20% for disease and 15 – 30% for pest.From the activities can be take several conclusions: (1) Extent of diseasein vegetable crops in this area is high, (2) The farmers do not yetunderstand the causes of pest and plant disease ( pathogens) that attacktheir vegetable crops, (3) The farmers do not understand the methods ofdisease control using biopesticide and botanic biopesticide, (4) Theextension and field practice, have improved farmers' knowledge aboutthe management of vegetable diseases using biopesticides and botanicbiopesticide
Biologi Penghisap Buah Helopeltis sp. (Hemiptera: Miridae) pada Buah Kakao dan Mentimun Novri Nelly; Ujang Khairul
JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) Vol 1 No 2 (2017): Desember 2017
Publisher : Jurusan Hama dan Penyakit Tumbuhan Faperta Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (237.703 KB) | DOI: 10.25077/jpt.1.2.62-67.2017

Abstract

This research purposed to study biology of Helopeltis sp. on cocoa and cucumber fruits. Research was done in insect bioecology laboratory using direct observation with 10 replications for each fruit. Parameter observed were copulation (hours), preoviposition time (days), number of eggs, nymphals, and adult periods (days), Oviposition time (days), and post oviposition time (days). The data obtained were analyzed descriptively and compared with t-test. Result of this research showed that Helopeltis sp. could thrive in cacao and cucumber fruits. Adult of Helopeltis sp. longevity in cacao fruits was 16 days and in cucumber fruits was 13 days. The number of eggs product average 10-10.9 eggs/female.
Perkembangan Temporal-Spatial Penyakit Layu Stewart (Pantoea stewartii subsp. stewartii) Pada Tanaman Jagung Temporal-Spatial Development of Stewart Wilt (Pantoea stewartii subsp. stewartii) on Corn Yulfi Desi; Trimurti Habazar; Ujang Khairul; Agustian Agustian
Jurnal Embrio Vol 10 No 1 (2018): Jurnal Embrio
Publisher : Fakultas Pertanian Universitas Tamansiswa Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (437.828 KB) | DOI: 10.31317/embrio.v10i1.269

Abstract

Penyakit layu Stewart merupakan penyakit penting pada tanaman jagung. Di Indonesia penyakit ini baru dilaporkan pada tahun 2008 dan pada tahun 2015 dinyatakan sebagai organisme pengganggu tumbuhan karantina (OPTK) kategori A2. Informasi tentang perkembangan dan penyebaran penyakit ini masih terbatas. Tujuan penelitian adalah: Mendapatkan model kurva perkembangan (temporal) dan pola penyebaran (spatial) penyakit layu Stewart pada tanaman jagung pada dua periode tanam. Percobaan lapangan dilaksanakan selama dua musim tanam di Nagari Koto Baru, Kecamatan Luhak Nan Duo, Kabupaten Pasaman Barat, Provinsi Sumatera Barat. Penelitian menggunakan bedengan berukuran 5,5 x 11,0 m dengan jumlah populasi 150 tanaman dengan 6 ulangan. Pengamatan meliputi insidensi penyakit (%) dan severitas penyakit (%). Model kurva perkembangan penyakit (temporal) diuji dengan model: logistic, monomolecular, dan exponential. Pola penyebaran penyakit (spatial) dianalisis dengan run dan diuji dengan uji Z: aggregate, regular, dan random. Hasil penelitian mendapatkan: Model kurva perkembangan (temporal) penyakit layu Stewart pada dua periode tanam yang berbeda adalah sama yaitu monomolecular dan Pola penyebarannya (spatial) juga sama yaitu random.
SELEKSI AKTINOBAKTERIA INDIGENOUS UNTUK PENGENDALIAN PENYAKIT HAWAR DAUN BAKTERI (Xanthomonas oryzae pv. oryzae) SERTA PENINGKATAN PERTUMBUHAN PADI Muhammad Fadil; Yulmira Yanti; Ujang Khairul
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 8, No 1 (2023): JURNAL AGROHITA
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v8i1.8178

Abstract

Aktinobakteria merupakan kelompok bakteri Gram postif, dominan di tanah dan memiliki peran penting dalam melindungi tanaman dari serangan patogen dan meningkatkan pertumbuhan tanaman. Tujuan penelitian untuk mendapatkan isolat Aktinobakteria yang dapat mengendalikan penyakit hawar daun bakteri dan peningkatan pertumbuhan tanaman padi. Penelitian terdiri 3 tahap yaitu 1.) Isolasi Isolat Aktinobakteria dan Xanthomonas oryzae pv. oryzae. Variabel yang diamati adalah ciri-ciri morfologi, uji Gram, reaksi hipersensitif, dan uji patogenisitas. 2.) Seleksi Aktinobakteria dalam meningkatkan pertumbuhan bibit padi dengan 27 perlakuan 6 ulangan, 25 isolat Aktinobakteria, 1 kontrol, dan 1 bakterisida berbahan aktif streptomisin, disusun dengan Rancangan Acak Lengkap. Variabel yang diamati adalah daya muncul lapang, tinggi bibit, jumlah daun, panjang akar, berat basah, dan berat kering. 3.) Seleksi Aktinobakteria untuk mengendalikan hawar daun bakteri pada tanaman padi dengan 21 perlakuan dan 6 ulangan, 18 isolat (hasil seleksi tahap II), 1 kontrol positif, 1 kontrol negatif, dan 1 kontrol positif, disusun dengan Rancangan Acak Lengkap. Variabel yang diamati adalah perkembangan penyakit dan pertumbuhan tanaman. Hasil penelitian diperoleh 30 hasil isolasi. Isolat Aktinobakteria terbaik dalam menekan perkembangan penyakit hawar daun bakteri dan memacu pertumbuhan tanaman padi adalah isolat dengan kode APRD 3I211, APRD 1I122, APRP 2S121, APRP 1I121, APRP 3I212 dengan Efektivitas 43.45%-50.69%.