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DISEMINASI TEKNOLOGI PUPUK KANDANG SAPI PLUS RIZOBAKTERI PADA KELOMPOK TANI KAKAO DI KABUPATEN LIMAPULUH KOTA Haliatur Rahma; Jumsu Trisno; Martinius Martinius; Reflin Reflin; Sri Wahyuni; Nusyirwan Nusyirwan
Jurnal Hilirisasi IPTEKS Vol 1 No 4.a (2018)
Publisher : LPPM Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (407.158 KB)

Abstract

Kegiatan diseminasi teknologi pupuk kandang sapi plus rizobakteri pada tanaman kakao dilakukan di Kecamatan Guguak dan Akabiluru Kabupaten Lima Puluh Kota yang dimulai dari bulan Maret sampai dengan November 2018. Kegiatan ini bertujuan untuk mengenalkan teknologi formulasi pupuk kandang plus rizobakteri pada kelompok tani kakao. Inovasi, buah lobek, maju sejahtera dan aroma menggunakan metode penyuluhan. Pentingny kegiatan ini dilakukan disebabkan oleh tingginya kejadian dan keparahan penyakit VSD di Kecamatan Guguak dan Akabiluru Kabuparten Limapuluh Kota yang mencapai 86,60%. Untuk itu, diperlukan teknologi pengendalian yang mudah dibuat, murah dan bahan baku yang mudah diperoleh disekitar petani. Penggunaan air kelapa merupakan salah satu perbanyakan bakteri Serratia maresescens dan Pseudomonas fluorescens yang nanti akan diformulasi dengan pupuk kandang. Kegiatan ini meliputi: pemantauan tingkat serangan OPT dilahan kelompok tani kakao, kemudian dilanjutkan dengan penyuluhan, pelatihan, dan evaluasi hasil kegiatan. Dari hasil kegiatan bahwa kegiatan ini mampu meningkatkan pengetahuan petani terhadap penggunaan biofertilizer yang berasal dari bakteri yang diformulasikan dengan air kelapa dan pupuk kandang, sehingga penggunaan formulasi ini mampu mengurangi biaya penggunaan pupuk kimia yang memiliki harga yang mahal.
Identifikasi Molekuler Begomovirus Penyebab Penyakit Kuning Keriting pada Tanaman Cabai (Capsicum annum L.) di Sumatera Barat Trisno, Jumsu; Hidayat, Sri Hendrastuti; Jamsari, Jamsari; Habazar, Trimuri; Manti, Ishak
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.888 KB) | DOI: 10.31258/jnat.13.1.41-46

Abstract

Pepper plants showing Begomovirus-like symtoms, consisting of yellowing, leaf curling, and distortion, werecollected from fields located in the Padang, West Sumatra. The aim of this research is to identification ofbegomovirus associated with yellow leaf curl diseases on pepper. Total DNA was extracted from infected leaftissue according to Doyle and Doyle (1999) with slight modification. Polymerase chain reaction (PCR) was used toamplify the coat protein region of the virus using universal degenerate primers pAV494 and pAC1048. The PCRamplified DNA product (approx. 560 bp) was sequenced. The nucleotide and amino acid sequences and BLASTsearch revealed highest homology with pepper yellow leaf curl Indonesia virus isolated pepper, tomato andAgeratum conyzoides from Java, but differences from those of tomato yellow leaf curl virus. The isolate was thententatively called pepper yellow leaf curl Indonesia virus-Padang (PYLCIV-Pdg).
Identifikasi Rizobakteria Asal Tanaman Cabai Berdasarkan Sekuen Gen 16S rRNA Jumsu Trisno; Yusniwati .
Jurnal Fitopatologi Indonesia Vol. 8 No. 3 (2012)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.476 KB) | DOI: 10.14692/jfi.8.3.79

Abstract

Rhizobacteria was reported to have a potential role as inducer of systemic resistance in several host plants. Five isolates of rhizobacteria (RbTD1-3, RbTD1-8, RbPdGN-3, RbAg1-5, RbLPK1-9) were isolated from rhizosphere of chillipepper plants in West Sumatera. Identification of rhizobacteria was conducted by polymerase chain reaction using primers 27F and 1525R which amplified 16S rRNA region. DNA fragment of 1.5 kb was succesfully amplified from 4 isolates (RbLPK1-9, RbPdGN-3, RbTD1-3 and RbTD1-8) and subjected to direct sequencing. Phylogenetic analysis based-on 16S rRNA sequences showed that isolate RbLPK1-9 has a close relationship with Pseudomonas fluorescen Biotipe G (92%), whereas isolates RbTD1-3, RbTD1-8 and RbPdGN-3 have a close relationship with Bacillus subtilis 10407 (57%).Key words: polymerase chain reaction, rhizobacteria, 16S rRNA
Detection and Sequence Diversity of Begomovirus Associated with Yellow Leaf Curl Disease of Pepper (Capsicum annuum) in West Sumatra, Indonesia JUMSU TRISNO; SRI HENDRASTUTI HIDAYAT; TRIMURTI HABAZAR; ISHAK MANTI; . JAMSARI
Microbiology Indonesia Vol. 3 No. 2 (2009): August 2009
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.3.2.2

Abstract

Yellow leaf curl disease of pepper has become an emerging important disease in West Sumatra since early 2000. Several attempts have been made, including disease survey and detection, in order to identify the causal agent of the disease. Pepper (Capsicum annuum) plants showing yellow leaf curl from West Sumatra were analyzed for presence of Begomovirus employing Polymerase Chain Reaction (PCR) with degenerate primers pAL1v 1978 and pARc 715. A DNA fragment of 1.6 kb was successfully amplified and subjected to direct sequencing. A stem loop region was found in the nucleotide sequence obtained, which contains the conserved nucleotide signature sequence TAATATTAC present in begomoviruses. Based on the stem loop region comparisons and phylogenetic analysis, the virus isolates from West Sumatra showed the closest relationship to Pepper yellow leaf curl Indonesia virus (PYLVIV) and Tomato yellow leaf curl Indonesia virus (TYLCIV). The sequence was different from other Asia Begomoviruses reported earlier. These isolates were divided into three groups which were tentatively called Pepper yellow leaf curl Indonesia virus-West Sumatra-[group 1], -[group 2] and -[group 3] {PYLCIV-WS-[group1], -[group2], and -[group3]}.
Biaya Penggunaan Paket Teknologi BP3T Pupuk Kandang dan Nano Pestisida Serai Wangi pada Tanaman Kakao di Kabupaten Lima Puluh Kota Sri Wahyuni; Haliatur Rahmai; Jumsu Trisno; Martinius Martinius; Rita Noveriza; Reflin Reflin; Sri Yuliani; Nusyirwan Nusyirwan
INDONESIAN JOURNAL OF ESSENTIAL OIL Vol 3, No 1 (2018)
Publisher : Institut Atsiri Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.42 KB)

Abstract

Teknologi BP3T (Bakteri Perakaran Pemacu Pertumbuhan Tanaman) pupuk kandang dirancang untuk membantu mengendalikan penyakit VSD (vascular streak dieback) yang menyerang tanaman kakao sekaligus membantu pertumbuhan tanaman. Penetian ini bertujuan menganalisis perbandingan biaya penggunaan pupuk kandang konvensional dengan teknologi BP3T Pupuk Kandang. Penelitian ini dirancang secara deskriptif kuantitatif yang berlokasi di Kabupaten Limapuluh Kota. Pengambilan sampel dilakukan secara sensus, yaitu 43 orang yang tergabung ke dalam 4 kelompok tani terpilih, yaitu Kelompok Tani Aroma, Kelompok Tani Inovasi, Kelompok Tani Buah Lobek, dan Kelompok Tani Maju Sejahtera. Biaya pupuk yang dikeluarkan petani sebelum menggunakan pupuk Formula BP3T lebih besar dibandingan dengan biaya penggunaan pupuk Formula BP3T. Dari kesimpulan yang ada, disaran kepada petani untuk menggunakan pupuk Formula BP3T dan mengembangkannya. Selain biayanya murah, pupuk Formula BP3T juga memilikik manfaat untuk meningkatkan ketahanan tanaman terhadap penyakit VSD.
Penyakit Kerupuk Tembakau di Sumatera Barat Jumsu Trisno; Rahil Ade Rifqah; Martinius Martinius
Jurnal Fitopatologi Indonesia Vol. 10 No. 6 (2014)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (723.159 KB) | DOI: 10.14692/jfi.10.6.210

Abstract

Tobacco leaf curl disease (“kerupuk”) is a new disease on tobacco in West Sumatra. This disease has spread throughout the tobacco planting area in District Limapuluh Kota with intensity of 2.5–26.3%. DNA fragment of 1600 bp was successfully amplified using polymerase chain reaction method with primer pairs for Begomovirus PAL1v 1978/PAR1c 715 from tobacco leaves showing symptoms. This result indicated Begomovirus infection. Sequence analysis based on NCBI BLAST program showed that Begomovirus infecting tobacco in West Sumatera has the highest homology of 89% and 91–97% with Pepper yellow leaf curl Indonesia Virus infecting tomato and chili pepper in West Sumatera, respectively. This is considered as the first report of Begomovirus infection on tobacco in West Sumatra.
Infeksi Ganda Pepper Yellow Leaf Curl Virus dan Chilli Veinal Mottle Virus dalam Menimbulkan Penyakit Daun Kuning Keriting Cabai Jumsu Trisno; Jamsari Jamsari; Sri Hendrastuti Hidayat
JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) Vol 5 No 2 (2021): Desember 2021
Publisher : Jurusan Hama dan Penyakit Tumbuhan Faperta Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.5.2.77-88.2021

Abstract

Yellow leaf curl disease is the primary disease in chili plants with up to 100% losses. Detection from the field showed a mixed infection between Geminivirus or Pepper Yellow Leaf Curl Virus (PYLCV) and several viruses, including Chilli Veinal Mottle Virus (ChiVMV) and Cucumber mosaic Virus (CMV). Therefore, this study aimed to determine the role of PYLCV and ChiVMV dual infection in influencing the development of chili yellow leaf curl disease. This study used a completely randomized design (CRD) with six treatments and ten replications. The treatments used were multiple Geminiviruses and ChiVMV infections simultaneously, before ChiVMV co-infection, after Geminivirus infection, single infection and whithout infection viruses. The results showed that the presence of ChiVMV infection before, after, and concurrently with Geminiviruses infection could increase the yellow leaf curl disease intensity and reduce plant height and wet weight growth. The mean scale value of ChiVMV infection before, after, and concurrently with Geminiviruses infection were 3.38, 3.90 and 3.58 compared to single Geminiviruses infection (scale of 3.20) and single ChiVMV (scale of 1.33). The interaction analysis of ChiVMV and geminiviruses infections based on the percentage reduction in plant growth height and wet weight showed additive interaction. ChiVMV co-infection further exacerbated the incidence of yellow leaf curl diseases in chilies.
Vascular Streak Dieback: Penyakit Baru Tanaman Kakao di Sumatera Barat Jumsu Trisno; Reflin Reflin; Martinius Martinius
Jurnal Fitopatologi Indonesia Vol. 12 No. 4 (2016)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.046 KB) | DOI: 10.14692/jfi.12.4.142

Abstract

Vascular streak dieback (VSD) symptoms was reported recently in several cacao plantations in West Sumatera.  Disease incidence reached 58.82–100% with disease intensity of 24.29–44.71%.  In some cases, dead plant was also found. Fungal isolation was performed to identify the agents associated with VSD.  Plant samples showing VSD symptoms was collected from 3 locations of cacao production center in West Sumatera, i.e. Limapuluh Kota regency, Padang Pariaman regency, and Padang city.  Small pieces of leaf and twig were plated on water agar and potato dextrose agar medium for fungal isolation.  Morphology of hifa, basidiocarp, and basidiospora observed from fungi colonies indicated the presence of Ceratobasidium theobromae on infected plant samples.  This is the first report on the association of C. theobromae on cacao in West Sumatera. 
POTENSI RIZOBAKTERI INDIGENUS SEBAGAI AGENS BIOKONTROL JAMUR FUSARIUM OXYSPORUM F. SP. CUBENSE PENYEBAB PENYAKIT LAYU TANAMAN PISANG Selviana Anggraini; Jumsu Trisno; Tizelia Tizelia
Jurnal Agroteknologi dan Pertanian (JURAGAN) Vol 1 No 1 (2020): Jurnal Agroteknologi dan Pertanian (JURAGAN) Oktober 2020
Publisher : LPPM Universitas Bina Insan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32767/juragan.v1i1.25

Abstract

The use of rhizobacteria is an alternative control in suppressing plant disease attacks. The purpose of this study was to obtain indigenous rhizobacteria which have the potential as antagonist agents of Fusarium oxysporum f. sp. cubense. Sampling was carried out randomly selected on endemic lands. Isolation of rhizobacteria using serial dilution method and its antagonist test against Fusarium oxysporum f. sp. cubense uses the dual culture method. The isolation results from 3 banana cultivars (kepok, raja sere, and mas) obtained 24 rhizobacterial isolates with different morphological and physiological characters. The antagonist test results showed that 8 rhizobacterial isolates were able to suppress the growth of Fusarium oxysporum f. sp. cubense and is not plant pathogenic.
SKRINING RHIZOBAKTERIA PENGHASIL INDOLE ANCETID ACID PENGINDUKSI PERTUMBUHAN DARI RHIZOSFIR CABAI (Capsicum annuum L.) Jumsu Trisno
Jurnal Agroekoteknologi Vol 3, No 1 (2011)
Publisher : Jurusan Agroekoteknologi Fakultas Pertanian Untirta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (279.121 KB) | DOI: 10.33512/j.agrtek.v3i1.569

Abstract

Rhizobacteria was a colonialist bacterial at rooting area that had capacity to produce IAA and enhance plant growth. Rhizobacteria indigenus chili had ability to produce IAA and induces various of plant growth. IAA production was not always linked with the ability to enhance plant growth. Obtained there were 11 isolates with various of morphological characters and had ability as RMSD (Plant Growt Promoting Rhizobacteria) with an effective increase in growth from 57.88 to 82.80%.Key words: Rhizobacteria, IAA, Growth, Chili Rhizosfer