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All Journal Alchemy : Journal of Chemistry BERITA BIOLOGI Pharmaceutical Sciences and Research (PSR)
Antonius Herry Cahyana
Departemen Kimia, FMIPA, Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Materials Science & Nanotechnology

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SKRINING DAN ISOLASI METABOLIT AKTIF ANTIBAKTERI KULTUR JAMUR ENDOFIT DARI TUMBUHAN Albertisia papuana Becc. [Screening and Isolation of Antibacterial Active Metabolite from the Culture of Endophytic Fungi from Albertisia papuana Becc.] Fathoni, Ahmad; Ilyas, Muhammad; Cahyana, Antonius Herry; Agusta, Andria
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8087.938 KB) | DOI: 10.14203/beritabiologi.v12i3.639

Abstract

Totally 15 isolates of endophytic fungi were obtained from leaves and young stems of a medicinal plant Albertisia papuana Becc.(Menispermaceae). The antibacterial screening of the ethyl acetate extract derived from the fungi cultures in potato dextrose broth (PDB)were performed on a non eluted Thin Layer Chromatography Bioautography assay (TLC bioautography assay). From the screening test, itwas found that the ethyl acetate extract of the fungus Xylaria sp. DAP-KRI-5 culture showed strong antibacterial activity against Staphylococcus aureus. The isolation of active metabolite based on the bioautography guided assay from ethyl acetate extract of the Xylaria sp. DAP-KRI-5, led us to identify phloroglucinol as a main antibacterial compound. The chemical structure of phloroglucinol was deduced from it spectral data, including UV-Vis, 1H and 13C-NMR, GC-MS and published data elsewhere.
Kopling Oksidatif Eugenol Menggunakan Ekstrak Enzim Peroksidase dari Akar Tanaman Sawi Hijau serta Uji Bioaktivitasnya Ardiansah, Bayu; Cahyana, Antonius Herry; Suwarso, Wahyudi Priyono; Maryana, Rianti; Merly, Siti
ALCHEMY Vol 5, No 3 (2017): ALCHEMY
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (630.772 KB) | DOI: 10.18860/al.v5i3.3776

Abstract

Peroxidase has been successfully extracted and partially purified from Brassica juncea L. Czern root with protein content and specific activity of 0.747 mg/mL and 0.244 U/mg, respectively. Then, it was applied as biocatalyst for green oxidative coupling reaction of eugenol to produce an orange crystal, dimeric form of eugenol (84% yield). Structure elucidation was done by using FTIR, UV-Vis and GC-MS instruments. The optical activity of dimeric eugenol was found to have Ra-configuration. Furthermore, biological activity of the product as antioxidant has been evaluated resulting in IC50 values of 32.14 and 42.43 ppm for dimeric and monomeric eugenol, respectively.
Enzim Papain: Aspek Green Chemistry pada Reaksi Knoevenagel Haryanto, Laurentius; Cahyana, Antonius Herry
Pharmaceutical Sciences and Research
Publisher : UI Scholars Hub

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Green chemistry aspect is the chemical approach that has been studied in the past two decade. One of the principles is the development of green synthesis process that is friendly for the environment. This research showed that papain can be used as catalyst for Knoevenagel reaction with 3 kinds of substituted-benzaldehyde and malononitrile as substrates in aqueous medium. The best reaction condition with 80% yield was reached by utilizing of 25 mg papain/mmol substrate. Reaction was conducted at ambient temperature and pressure for 30 min. Products were as yellowish to yellow needle crystals and successfully characterized by melting point, UV-Vis, IR, mass spectra, and 13C & 1H-NMR, named as 2-(4-hydroxybenzylidene)-malononitrile); 2-(3-hydroxybenzylidene)-malononitrile); and 2-(4-hydroxy-3-methoxybenzylidene)-malononitrile).
Co-Authors Ahmad Fathoni Aliya Nur Hasanah ANDRIA AGUSTA Ardiansah, Bayu Laurentius Haryanto Laurentius Haryanto, Laurentius Maryana, Rianti Merly, Siti Muhammad Ilyas