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Effects of pH, Nacl and Teating on the Antibacterial Stability of Kecombrang Rifda Naufalin; Betty Sri Laksmi Jenie; Feri Kusnandar; Mirnawati Sudarwanto; Herastuti Sri Rukmini
Jurnal Teknologi dan Industri Pangan Vol. 17 No. 3 (2006): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

The effect of pH (4-9), NaCl concentration (1-5%), temperature and heating time (80, 100 and 1210C for 10, 20 and 30 minute) on the antibacterial effectivity of ethyl acetate and ethanol kecombrang extract were analysed. Both ethyl acetate and ethanol extracts showed antibacterial activity at pH 4-8, but its activity gradually decreased at higher pH. At pH 9, only ethanol extract still showed antibacterial activity. Addition of 1-4% NaCl on ethyl acetate and ethanol extract still showed antibacterial activity. Heating the extracts at 80-100 0C for 10-30 minutes and 1210C for 10 minutes did not haves significantly affect on the antibacterial activity of both ethyl acetate and ethanol extracts. Application of ethyl acetate extract at the concentration of 1 and 3 MIC on minced meat were still effective to reduce the viable bacteria until 7 days and 5 MIC was still effective until 9 days. Key words : Kecombrang, pH, NaCl, temperature and heating time, antibacteria
Antibacterial Activity of Kecombrang Flower Extract Toward Pathogenic and Food Spoilage Bacteria Rifda Naufalin; Betty Sri Laksmi Jenie; Feri Kusnandar; Mirnawati Sudarwamto; Herastuti Rukmini
Jurnal Teknologi dan Industri Pangan Vol. 16 No. 2 (2005): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

In this study, kecombrang flowers was extracted with non polar (hexane), semipolar (ethil acetate) and polar (ethanol) solvent. The result revealed that ethil acetat and ethanol extracts inhibited 7 bacteria, i.e : spore forming bacteria (Bacillus cereus), Gram positive bacteria (Staphylococcus aureus) and (Listeria monocytogenes), Gram negative bacteria (Salmonella typhimurium, Aeromonas hydrophila and Escherichia coli), spoilage bacteria (Pseudomonas aeruginosa) but did not inhibit Lactobacillus plantarum. The hexane extract did not show antimicrobial activity. On well diffusion test, ethil acetate developed clear zones of 12.3 – 27.3 mm (diameters) and this was higher than ethanol extract 11.0-15.4 mm (diameters). The MIC (minimum inhibitory concentration) of ethil acetate and ethanol extract against the seven bacteria were 3-13 mg/ml. Key words : Kecombrang (Nicolia speciosa), antibacteria, extract
Iodine Fortification on Brown Sugar : The Effect of Fortification Period and Source of Iodine Rifda Naufalin; Budi Sustriawan; Poppy Arsil
Jurnal Teknologi dan Industri Pangan Vol. 15 No. 3 (2004): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Gula kelapa is a traditional and potencial comodity in Banyumas. Fortification with iodium in gula kelapa should be criteria were iodium in product over than 30 ppm bk. Fortification process was need a long thermal process. Stability of iodium in gula kelapa has been studied. The experimental method used Randomized Block Design. The factors analyzed were three kinds of fortification (fresh nira, nira prosessed, nira cristalization) and four kinds of iodium (control, NaI, KI, and KIO3) with 3 replications, and total were 36 unit. The result of the research showed that fortification when nira after endpoint with kalium iodida is the best interaction, iodium content is 34,89 ppm db. Key words : Brown sugar, fortification, iodine.
Alteration of coconut sap quality due to the addition of natural preservatives Anna Sulistyaningrum; Tri Yanto; Rifda Naufalin
Jurnal Penelitian Pascapanen Pertanian Vol 12, No 3 (2015): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v12n3.2015.137-146

Abstract

Coconut sap is easily fermented during storage, so it is necessary to add preservative. Farmers usually use a solution of lime with mangosteen rind to preserve the sap. The availability of these preservatives are limited, so many farmers used synthetic preservatives like sodium metabisulfite. Some natural preservatives that can be used as an alternative to extend the shelf life of coconut sap were betel leaves, clove leaves, guava leaves, secang wood, and tea leaves. These materials have bioactive components that possess antimicrobial activity.This research was aimed to 1) determine the effect of types and concentration of natural preservatives to preserve freshness of coconut sap and brown sugar qualities, 2) determine the effect of the storage time on the quality of coconut sap. The research consisted of two stages and used experimental method with Randomized Block Design. The treatment of first stage included the type of natural preservative (M), consentration of preservative (K), and storage duration (L), while the second stage consisted of the best three natural preservatives from first stage (S) and concentration of preservatives (R). The results of this research showed that the coconut sap that was added with clove leaves (M4) with concentration of 4.5% (K1) gave highest pH value, levels of sucrose, sensory value, and inhibition of both the storage of 4 and 8 hours, followed by guava leaves (M5) and mangosteen rind (M1). Brown sugar is added three types of preservatives showed that no difference on water content, ash content, reduction sugar and total sugar content. These parameters meet SNI standard, except ash content only mangosteen rind and clove leaf that meets SNI standard. PERUBAHAN KUALITAS NIRA KELAPA AKIBAT PENAMBAHAN PENGAWET ALAMINira kelapa mudah terfermentasi selama penyimpanan, oleh karena itu diperlukan proses pengawetan. Petani biasanya menggunakan larutan kapur dengan kulit manggis untuk mengawetkan nira. Ketersediaan dari pengawet tersebut terbatas, sehingga banyak petani yang menggunakan bahan pengawet sintetis seperti natrium metabisulfit. Beberapa pengawet alami yang dapat dijadikan sebagai alternatif untuk memperpanjang umur simpan nira antara lain daun sirih, daun cengkeh, daun jambu biji, kayu secang, dan daun teh. Bahan-bahan tersebut memiliki komponen bioaktif yang mempunyai aktivitas antimikroba. Penelitian ini bertujuan untuk 1) mengetahui pengaruh jenis dan konsentrasi bahan pengawet alami dalam mempertahankan kesegaran nira dan kualitas gula merah, 2) mengetahui pengaruh lama penyimpanan terhadap kualitas nira kelapa. Penelitian ini terdiri dari dua tahap dan dilakukan dengan menggunakan metode eksperimen Rancangan Acak Kelompok (RAK). Perlakuan pada tahap pertama meliputi jenis pengawet alami (M), konsentrasi pengawet (K) dan lama simpan (L), sedangkan pada tahap kedua meliputi tiga jenis pengawet alami terbaik dari tahap pertama (S) dan konsentrasi pengawet (R). Hasil penelitian ini menunjukkan bahwa nira yang ditambah pengawet daun cengkeh (M4) dengan konsentrasi 4,5% (K1) memberikan nilai pH, kadar sukrosa, nilai sensori dan mempunyai daya hambat tertinggi baik pada penyimpanan 4 dan 8 jam, diikuti oleh pengawet daun jambu biji (M5) dan kulit manggis (M1). Gula merah yang ditambahkan dengan ketiga jenis pengawet terbaik tidak menunjukkan perbedaan terhadap kadar air, kadar abu, kadar gula reduksi dan kadar gula total. Ketiga parameter memenuhi standar SNI, kecuali kadar abu hanya pengawet kulit manggis dan daun cengkeh yang memenuhi standar SNI.