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Odang Carman
Departemen Budidaya Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor
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Feminization of raibow Iriatherina werneri (Meiken, 1974) using estradiol-17β hormone Rodhi Firmansyah; Odang Carman; Dinar Tri Soelistyowati
Jurnal Iktiologi Indonesia Vol 16 No 3 (2016): October 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i3.26

Abstract

Feminization of the rainbow (Iriatherina werneri) is an initial step to get the functional females (XY). If those functional females crossbreeding with normal males (XY), we will produce super males (YY) individuals. This study aimed to evaluate the optimum condition of feminization on I. werneri using estradiol-17β hormone treatment at different doses and immersion duration with a completely randomized factorial design which consists of different doses and immersion durations with three replicates. The data were analyzed statistically ( ANOVA ). Eyed stage embryos were immersed in 0, 200, 400 and 600 μg L-1 of estradiol-17β for six, 12 and 18 hours; and then the larvae were reared up to 70 days. The results showed that estradiol-17β treatments could increase the percentage of I. werneri female. The doses of 400 and 600 μg L-1 for both six and 12 hours immersion could increase the percentage of female significantly (p<0.050) with value ranged from 85,56-92,22%. The duration of estradiol-17β immersion significantly affected (p<0,05) the I. werneri survival rate. The duration of immersion for six hours showed the highest survival rate. On the other hand, both of doses and duration of immersion did not affect the hatching rate of I. werneri (p>0.05) Abstrak Pembetinaan ikan pelangi (Iriatherina werneri) adalah langkah awal untuk mendapatkan individu betina fungsional (XY). Jika individu betina fungsional ini dikawinkan dengan jantan normal (XY) akan menghasilkan individu ikan jantan super (YY). Penelitian ini bertujuan untuk mengevaluasi kondisi optimum dosis dan lama perendaman yang berbeda untuk pembetinaan ikan pelangi dengan menggunakan hormon estradiol-17β yang dirancang menggunakan rancangan acak lengkap pola faktorial yang terdiri atas faktor dosis dan lama perendaman masing-masing diulang tiga kali kemudian data dianalisis secara statistik (ANOVA). Telur ikan pelangi stadia embrio bintik mata direndam dalam larutan estradiol-17β dosis 0, 200, 400 dan 600 μg L-1 selama 6, 12, dan 18 jam, kemudian larva dipelihara selama 70 hari. Hasil penelitian menunjukkan bahwa perlakuan estradiol-17β dapat meningkatkan persentase betina; dosis 400 dan 600 μg L-1 selama 6 dan 12 jam meningkatkan persentase betina secara nyata (p<0,05) dengan nilai 85,56-92,22%. Lama perendaman berpengaruh nyata (p<0,05) terhadap tingkat kelangsungan hidup dan perendaman selama enam jam memberikan hasil yang terbaik. Sementara itu, dosis dan lama perendaman tidak memengaruhi tingkat penetasan (p>0,05).
Spawning behavior, female reproductive potential and breeding technique optimize of threadfin rainbowfish Iriatherina werneri Muh. Herjayanto; Odang Carman; Dinar Tri Soelistyowati
Jurnal Iktiologi Indonesia Vol 16 No 2 (2016): June 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i2.39

Abstract

Basic information of threadfin rainbowfish especially their reproduction in captivity is little known. Therefore, study on the spawning behaviour and female reproductive potential based on the different treatment of feed (commercial food and natural food) and optimize breeding technique is needed. The study on optimize breeding technique including the effect of different spawning systems (masse or individual), sex ratio (1:1, 1:2 and 1:3), and female size (small, medium and large) to support threadfin rainbowfish culture. The result showed that the threadfin rainbowfish spawn at 13-15 hours after pairing broodfish and the fertilization starts when male spread out and shrink up the fins. The eggs were released faster in the masse than in individual spawing systems and the eggs were released more simultaneously in the morning (94.92%). The threadfin rainbowfish is a partial spawner that spawns every day until 30 days. The potential of eggs and larvae production could be increased up to 4 and 14 times by fed the fish with natural food. Technique for optimize the breeding is using the masse spawning system with sex ratio 1:3 and size range of female is 26.98 to 35.76 mm. Abstrak Informasi reproduksi ikan pelangi Iriatherina werneri pada wadah terkontrol masih sedikit diketahui. Oleh karena itu, diperlukan kajian mengenai tingkah laku memijah, potensi reproduksi ikan betina berdasarkan perbedaan pakan (buatan dan alami), dan optimasi teknik pemijahan. Kajian optimasi pemijahan meliputi pengamatan pengaruh perbedaan sistem pemijahan (massal atau individual), perbedaan rasio kelamin pemijahan jantan : betina (1:1, 1:2, dan 1:3), serta perbedaan ukuran betina (kecil, sedang, dan besar) untuk mendukung kegiatan budi daya. Hasil penelitian menunjukkan bah-wa pemijahan ikan pelangi terjadi pada 13-15 jam sejak pemasangan ikan jantan dan betina yang diawali oleh gerakan ikan jantan mengembangkan dan menguncupkan sirip. Telur yang dikeluarkan pada pemijahan massal berakhir dua jam lebih cepat dibandingkan pemijahan individual dan telur lebih serempak dikeluarkan pagi hari (94,92%). Ikan pelangi merupakan pemijah bertahap yang mampu memijah setiap hari selama 30 hari. Potensi jumlah telur dan larva yang di-hasilkan seekor betina dapat ditingkatkan masing-masing sebanyak empat kali lipat dan 14 kali lipat melalui pemberian pakan alami. Optimasi teknik pemijahan I. werneri dicapai dengan menggunakan sistem massal dengan rasio kelamin 1: 3 dan menggunakan ikan betina berukuran 26,98-35,76 mm.
Phenotypes performance of tilapia best, nirwana II, jatimbulan, and sultana using floating net, and pond culture system Ibrahim Satrio Faqih; Dinar Tri Soelistyowati; Odang Carman
Jurnal Iktiologi Indonesia Vol 15 No 3 (2015): October 2015
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v15i3.55

Abstract

Tilapia is one of the introduced fish species in Indonesia and was firstly imported from Taiwan in 1969. Since that time, many efforts have been made to increase its quality through genetic improvement. Some strains of tilapia have been successfully generated such as best, nirwana II, jatimbulan and sultana. In sustainable aquaculture, success of culture production depends on good environmental conditions and water quality. The aim of this study was to evaluate the phenotype performances of four strains of tilapia obtained through selective breeding reared in floating net and ponds. A completely randomized designs with two factors were used in this research, i.e. strains of tilapia and culture system with three replicates. Fifty fish with an average body length of 3-5 cm was reared in ponds and floating net 2 x 2 x 1 m, fed daily in the morning and afternoon during the experiment. Twenty fish from each strain were sampled in every two weeks and fish body length and body weight were measured. Truss morphometric measurement was conducted after 12 weeks of rearing process. The results showed that sultana strain has the highest growth rate, jatimbulan and best strains have the similar survival and feed conversion rates, while the highest biomass was found in the best strain. The nirwana II has the lowest survival rate (18%), but this strain showed the highest feed efficiency. Culture system affects the phe-noltypic variance of truss morphometric, viz. eleven characters in floating net and two characters in pond specimens. Abstrak Nila di Indonesia merupakan ikan introduksi yang didatangkan dari Taiwan pertama kali pada tahun 1969. Dalam peri-ode yang cukup lama, upaya peningkatan kualitas benih ikan dilakukan secara terus menerus melalui perbaikan mutu genetik. Beberapa strain ikan nila yang telah dihasilkan di antaranya nila best, nirwana II, jatimbulan, dan sultana. Da-lam kegiatan perikanan budi daya yang berkelanjutan, faktor lingkungan dan kualitas perairan merupakan pembatas ke-berhasilan usaha budi daya. Tujuan penelitian ini adalah untuk mengevaluasi keragaan fenotipe empat strain ikan nila hasil pemuliaan pada sistem budi daya karamba jaring apung (KJA) dan kolam air tenang. Penelitian ini dirancang menggunakan rancangan acak lengkap pola faktorial yang terdiri atas faktor empat strain ikan nila dan faktor sistem bu-di daya yaitu KJA dan kolam air tenang. Setiap perlakuan diulang tiga kali. Tiap wadah kolam dan KJA ber-ukuran 2 x 2 x 1 m3 dimasukkan benih sebanyak 50 ekor berukuran 3-5 cm, dan diberi pakan pada pagi dan sore selama masa pe-meliharaan. Setiap dua minggu dilakukan sampling masing-masing strain 20 ekor untuk pengamatan panjang dan bo-bot. Setelah 12 minggu pemeliharaan dilakukan pengukuran truss morfometrik. Hasil penelitian menunjukkan bahwa ikan nila strain sultana memiliki laju pertumbuhan tertinggi, jatimbulan memiliki tingkat kelangsungan hidup dan kon-versi pakan yang sama dengan best, biomassa tertinggi dicapai pada nila best, sementara nirwana II memiliki kelang-sungan hidup yang paling rendah (18%) namun memiliki efisiensi pakan yang terbaik. Sistem pemeliharaan memenga-ruhi peningkatan ragam fenotipe truss morfometrik yakni 11 karakter di KJA dan dua karakter di kolam air tenang.
Kloning promoter P-actin ikan mas, Cyprinus carpio Lin. 1758 dan analisis fungsionalnya menggunakan gen target protein pendaran hijau (GFP) Andi Aliah Hidayani; Odang Carman; Alimuddin Alimuddin
Jurnal Iktiologi Indonesia Vol 13 No 2 (2013): Desember 2013
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v13i2.101

Abstract

Promoter in the expression vector plays an important role on regulating of gene expression in transgenic fish. In fish transgenesis, researcher convinced that the use of all-fish expression vector is safety and prospective. The study was performed to isolate P-actin promoter, the promoter which has ubiquitous, constitutive, housekeeping characteristics, of common carp as a first step to construct all-common carp expression vector. Common carp P-actin promoter (ccBA) was isolated using PCR method with FBP1, RBP1, and RBP2 primers. Sequencing was performed using ABI PRISM 3100 machine, and analysis of sequences was conducted using GENETYX version 7 software. The results of sequence analysis showed that the length of DNA fragment obtained was approximately 1.5 kb. Results of homology with P-actin promoter sequence of a gene bank database (Accession No.: M24113) was 97.5%. The evolutionary conserved of transcription factor for P-actin promoter including CCAT, CArG, and TATA boxes were found in the sequence. Ubiquitous and higher expression of green fluorescent protein driven by ccBA promoter in muscle of common carp larvae was detected. It is most likely that the isolated sequence is a common carp P-actin promoter. Abstrak Promoter dalam vektor ekspresi berperan penting dalam mengatur ekspresi gen pada ikan transgenik. Dalam transgenesis ikan, peneliti yakin bahwa penggunaan vektor ekspresi semua ikan aman dan prospektif. Penelitian ini dilakukan untuk mengisolasi promoter P-aktin, promoter yang memiliki karakteristik ubiquitous, constitutive, house keeping, dari ikan mas sebagai langkah awal untuk mengkonstruksi vektor ekspresi semua ikan mas. Promoter P-aktin ikan mas (ccBA) diisolasi menggunakan metode PCR dengan primer FBP1, RBP1, dan RBP2. Sequensing dilakukan dengan menggunakan mesin ABI PRISM 3100, dan analisis sekuen dilakukan dengan menggunakan perangkat lunak GENE-TYX versi 7. Hasil analisis sekuen menunjukkan bahwa panjang fragmen DNA yang diperoleh adalah sekitar 1,5 kb. Hasil homologi dengan sekuen promoter P-aktin dari pangkalan data bank gen (No. Aksesi: M24113) adalah sebesar 97,5%. Faktor transkripsi yang tetap secara evolusioner untuk promoter P-aktin promoter termasuk CCAT, CArG, dan boks TATA ditemukan dalam sekuen. Ubiquitous dan ekspresi tertinggi protein pendaran hijau (GFP) dikendalikan oleh promoter ccBA dalam otot larva ikan mas yang dideteksi. Dengan demikian, kemungkinan besar bahwa sekuen yang terisolasi adalah promoter P-aktin ikan mas.
STUDI KROMOSOM IKAN PELANGI (Melanotaenia lacustris) [Chromosome Study of Rainbowfish (Melanotaenia lacustris)] Djamhuriyah S. Said; Odang Carman; Hidayat Hidayat; Abinawanto Abinawanto
Jurnal Iktiologi Indonesia Vol 3 No 2 (2003): Desember 2003
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v3i2.262

Abstract

Cytogenetic study of Melanotaenia lacustris was focussing on karyotype and to find out the information of chromosome. The research was conducted in Laboratory of Fish Breeding and Genetics, Faculty of Fisheries and Marine Science-IPB on May==December 2000. Chromosome plates were prepared by solid tissue technique and analyzed after staining with giemsa solution. Diploid chromosomes number of M. lacustris are (2n = 46). Karyotyping of this fish shown that 46 chromosomes consist of 9 pairs submetacentrik (SM) (no. 1, 3, 6, 7, 8, 9, 10, 13, and 14); 3 pairs subtelocentric (ST) (no. 4, 12, 22) and 10 pairs telocentric (T) (no. 2, 5, 11, 15, 16, 17, 18, 19, 20, and 21) with 1 ST and 1 T on the no. 23. Therefor is suggested that M. lacustris has a sex chromosome.AbstrakPenelitian sitogenetika pada ikan pelangi (Melanotaenia lacustris) difokuskan pada pengungkapan keanekaragaman kromosom dalam hal jumlah, bentuk, dan karyotipenya. Penelitian dilakukan di Laboratorium Genetika dan Reproduksi ikan Fakuitas Perikanan dan 11 mu Kelautan IPB, pada bulan on Mei — Deseber 2000. Preparat kromosom dibuat dengan metode jaringan padat yang menggunakan larva ikan umur 10-30 hari. Analisis kromosom dilakukan setelah pewarnaan dengan larutan Giemza. Kromosom diploid ikan M. lacustris adalah (2N = 46). Karyotipenya menunjukkan 46 kromosom yang terdiri atas 9 pasang berbentuk submetasentrik (SM) (no. 1, 3, 6, 7, 8, 9, 10, 13, and 14); 3 pasang subtelosentrik (ST) (no. 4, 12, 22) dan 10 pasang telosentrik (T) (no. 2, 5, 11, 15, 16, 17, IB, 19, 20, and 21) dengan 1 ST dan 1 T pada no. 23. Dari basil tersebut diduga bahwa M, lacustris memiliki kromosom sek.
Ploidy level determination in genetically modified polyploid striped catfish Pangasianodon hypophthalmus Sauvage, 1878 based on the number of nucleoli per cell Muhammad Sami Daryanto; Odang Carman; Dinar Tri Soelistyowati Soelistyowati; Rahman Rahman
Jurnal Iktiologi Indonesia Vol 19 No 1 (2019): February 2019
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v19i1.405

Abstract

The variation in the maximum number of nucleoli per cell in diploid and tetraploid striped catfish Pangasianodon hypophthalmus Sauvage, 1878 in attempts to verify the validity of nucleoli counting as an indirect method for polyploidy identification in fish. The aims of this research is to determine ploidy level of striped catfish based on maximum number of nucleoli per cell. Diploid striped catfish was produced by fertilizing eggs without thermal-shock and tetraploid fish was obtained by fertilizing eggs and applying thermal-shock induction with 4oC for 25 minutes at zygotic age 28 minutes after fertilization prior to first cleavage stage of zygote. The hatching rate of diploid group and tetraploid group were 81,35±0,73% and 3,39±1,78% and survival rate during 15 days rearing were 88,67±5,25% and 83,33±5,73%, respectively. The frequency of one, two, three, and four nucleoli per cell were counted based on each sample observation of 450-550 cells. Cells of diploid individuals had one, and maximum two nucleoli per cell, while tetraploid there were one, two, three, and maximum four nucleoli per cell. Anomaly in the silver-stained appearance at the maximum number of three nucleoli per cell indicated tetraploid individuals as verified by chromosome counting method. Ploidy level determination of striped catfish using the number of nucleoli per cell has a potential for rapid identification. Abstrak Penentuan variasi jumlah maksimum nukleolus per sel pada individu diploid dan tetraploid patin siam Pangasianodon hypophthalmus Sauvage, 1878 dilakukan dalam upaya verifikasi ketepatan penghitungan nukleolus sebagai metode tidak langsung dalam identifikasi ikan poliploid. Penelitian ini bertujuan menentukan tingkat ploidi ikan patin siam berdasarkan jumlah maksimum nukleolus per sel. Diploid patin siam dihasilkan melalui fertilisasi buatan antara telur dan sperma tanpa kejutan suhu sedangkan tetraploid patin siam dihasilkan melalui fertilisasi antara telur dan sperma serta di induksi menggunakan kejutan suhu 4oC dengan durasi kejutan 25 menit pada umur zigot 28 menit setelah fertilisasi sesaat sebelum mitosis I. Persentase penetasan kelompok perlakuan diploid 81,35±0,73% dan kelompok perlakuan tetraploid 3,39±1,78%. Sintasan kelompok perlakuan diploid 88,67±5,25% dan kelompok tetraploid 83,33±5,73% selama 15 hari pemeliharaan. Frekuensi satu, dua, tiga dan empat nukleoli per sel dihitung berdasarkan pengamatan 450-550 sel setiap individu yang diamati. Sel individu diploid memiliki satu dan maksimum dua nukleoli per sel, sedangkan individu tetraploid memiliki satu, dua, tiga, dan maksimum empat nukleoli per sel. Anomali muncul melalui pewarnaan perak nitrat pada jumlah maksimum tiga nukleoli per sel menunjukkan individu tersebut tetraploid yang diverifikasi menggunakan metode penghitungan kromosom. Penentuan ploidi pada ikan patin siam menggunakan penghitungan jumlah nukleolus per sel memiliki potensi dalam identifikasi secara cepat.
Cold temperature shock tetraploidization of striped catfish Pangasianodon hypophthalmus (Sauvage, 1878) with different of temperature and age of zygote Alfis Syahril; Odang Carman; Dinar Tri Soelistyowati
Jurnal Iktiologi Indonesia Vol 20 No 1 (2020): February 2020
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v20i1.454

Abstract

Striped catfish (Pangasianodon hypophthalmus) has a relatively slow growth, so the operational cost of production is high and incomparable with the selling price, thus makes the cultivation of striped catfish is inefficient. Genetic quality improvement through polyploidization is to produce sterile triploid fish (3n) that can overcome this problem. The provision of triploid fish is more efficient through tetraploidization. Tetraploidization in striped catfish using cold shock treatment has never been done before. The purpose of this study was to determine the optimum tetra-ploidization condition using cold shock with temperature and different age of zygote in striped catfish. A completely randomized factorial design with two treatments i.e. three level of temperatures (8oC, 12°C, 16oC ) and two different ages of zygote (29 and 31 minutes after fertilisation-maf) and three replications was used. In addition, a control without applying temperature shock treatment was performed, Soaking process was conducted for 30 minutes. Tetraploid identification was done by calculating the maximum total of nucleoli per cell that was confirmed by calculating the total of chromosome. The results showed that the degree of hatching rate and abnormalities has a significantly different effect (P <0.05). Number of nucleoli per cell was 4 and chromosomes was 112 (4n=112) for tetraploid), whereas for diploid fish the maximum number of nucleoli per cell was 2 and the number of chromosomes was 56 (2n=56). The highest tetraploid percentage was obtained at a treatment of 12oC at age of zygote 29 maf. Thus, the optimum condition of tetraploidization in striped catfish is using cold shock treatment of 12oC with the age of zygote of 29 maf. Abstrak Ikan patin siam (Pangasianodon hypophthalmus) memiliki pertumbuhan yang relatif lambat sehingga biaya operasional produksi tinggi tidak sebanding dengan rendahnya harga jual yang mengakibatkan budidaya ikan patin siam tidak efisien. Perbaikan mutu genetik melalui poliploidisasi yaitu untuk menghasilkan ikan triploid (3n) yang bersifat steril dapat mengatasi masalah tersebut. Penyediaan ikan triploid lebih efisien melalui tetraploidisasi. Tetraploidisasi pada ikan patin siam menggunakan perlakuan kejutan dingin belum pernah dilakukan sebelumnya. Penelitian ini bertujuan untuk menentukan kondisi optimum tetraploidisasi menggunakan kejutan dingin dengan suhu dan umur zigot berbeda pada ikan patin siam. Rancangan percobaan pada penelitian ini adalah Rancangan Acak Lengkap faktorial dengan dua faktor dan tiga ulangan, yaitu suhu 8oC, 12oC,16oC dan umur zigot 29 dan 31 msf (menit setelah fertilisasi) dengan perendaman selama 30 menit dan satu perlakuan kontrol (tanpa pemberian kejutan suhu). Identifikasi tetraploid dilakukan dengan menghitung jumlah maksimum nukleolus per sel yang dikonfirmasi dengan penghitungan jumlah kromosom. Hasil penelitian menunjukkan bahwa derajat penetasan dan abnormalitas berbeda nyata (P<0,05). Hasil pengamatan pada ikan tetraploid diperoleh jumlah maksimum nukleoli adalah 4 per sel dan jumlah kromosom yaitu 112 (4n=112), sedangkan pada ikan diploid adalah 2 per sel dan jumlah kromosom yaitu 56 (2n=56). Persentase tetraploid tertinggi diperoleh pada perlakuan suhu 12oC umur zigot 29 msf. Disimpulkan bahwa kondisi optimum tetraploidisasi pada ikan patin siam menggunakan kejutan dingin pada suhu 12oC dengan umur zigot 29 msf.
Performance of the third generation striped catfish, Pangasianodon hypophthalmus Sauvage, 1878 as results of the selection for bodyweight character in Freshwater Aquaculture Fisheries Center, Sungai Gelam, Jambi Irwan Irwan; Dinar Tri Soelistyowati; Odang Carman; Ronny Rachman Noor
Jurnal Iktiologi Indonesia Vol 19 No 3 (2019): October 2019
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v19i3.469

Abstract

Selective breeding of striped catfish (Pangasianodon hypophthalmus Sauvage, 1878) has been conducted at BPBAT Sungai Gelam to produce a growth line with mass selection method. Until 2018, the selective breeding program has produced three generations. Therefore, it was necessary to evaluate the performance of the third generation growth line (G3Ps) at the reproductive, seed and grow-out phase compared to the second generation the base population (G2Ds). A total of 10 pairs of broodstock (G3Ps and G2Ds) were spawned then the seeds were raised for 120 days in the media with regular water exchange (first treatment: T1) and without water exchange (second treatment: T2). The results showed that the number of eggs per gram of G3Ps (1600±124 eggs g-1) was significantly different from G2Ds (1490±101 eggs g-1). The bodyweight of G3Ps seeds at aged 40 days larger than G2Ds with selection response is 32.25%, while survival and feed efficiency were not significantly different. At the grow-out phase, between treat-ments were not significantly different and there was no interaction between lines and treatments for all the characters measured (p>0.05). The bodyweight of G3Ps was larger than the G2Ds with response selection 18.41% in T1 and 42.6% in T2. The control used was the base population so that the selection response obtained was an accumulation of three generations. Thus the selection response per generation was 6.14% in T1 and 14.20% in T2 measured at 162 days from hatching. It can be concluded that there is an improvement in the character of bodyweight for the third generation of growth line (G3Ps) as results of the selection at BPBAT Sungai Gelam both in good (T1) and bad environment (T2). Abstrak Seleksi ikan patin siam (Pangasianodon hypophthalmus Sauvage, 1878) telah dilakukan di BPBAT Sungai Gelam untuk meningkatkan pertumbuhan dengan metode seleksi individu. Sampai tahun 2018, seleksi tersebut telah meng-hasilkan tiga generasi. Penelitian ini bertujuan untuk mengevaluasi performa generasi ketiga galur pertumbuhan (G3Ps) pada tahap reproduksi, pertumbuhan benih, dan ukuran konsumsi dibandingkan dengan populasi dasar gene-rasi kedua (G2Ds). Sebanyak 10 pasang induk G3Ps dan G2Ds dipijahkan kemudian benih yang dihasilkan dibesarkan selama 120 hari pada media dengan pergantian air secara berkala (perlakuan pertama: T1) dan tanpa pergantian air (perlakuan kedua: T2). Hasil penelitian menunjukkan bahwa jumlah telur per gram adalah 1600±124 butir pada G3Ps, berbeda nyata dengan G2Ds yaitu 1490±101 butir. Benih G3Ps umur 40 hari memiliki bobot tubuh lebih besar diban-dingkan G2Ds dengan respons seleksi sebesar 32,25%, sedangkan sintasan dan efisiensi pakan tidak berbeda nyata. Pada tahap pembesaran ukuran konsumsi, antar perlakuan tidak berbeda nyata dan tidak ada interaksi antargalur dan perlakuan untuk karakter bobot tubuh, panjang baku, sintasan dan efisiensi pakan (p>0,05). Karakter bobot tubuh G3Ps lebih besar dibandingkan dengan G2Ds dengan respons seleksi total untuk tiga generasi sebesar 18,41% pada T1 dan 42,6% pada T2. Dengan demikian respons seleksi per generasi sebesar 6,14% pada T1 dan 14,20% pada T2 yang diukur pada umur 162 hari dari menetas. Disimpulkan bahwa terjadi perbaikan pada karakter bobot tubuh untuk galur pertumbuhan generasi ketiga (G3Ps) hasil program seleksi di BPBAT Sungai Gelam baik pada lingkungan baik (T1) maupun lingkungan buruk (T2).
Production of albino slayer through a combination of crossing in brushmouth (Hypostomus plecostomus, Linnaeus 1758) ornamental fish Ahmad Teduh; Dinar Tri Soelistyowati; Odang Carman; Harton Arfah
Jurnal Iktiologi Indonesia Vol 20 No 1 (2020): February 2020
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v20i1.510

Abstract

The objectives of this research was to evaluate the inheritance of albino slayer phenotype in brushmouth (Hypostomus plecostomus, Linn 1758) through the crosses and reproduction performance. The crossing scheme consisted of reciprocal crosses between albino slayer and albino non slayer (SN, NS) and between albino slayer (SS), each spawning in pairs (1:1) with three replications and twice spawning. The fish used in this study were brushmouth fish (Hypostomus sp.) albino Slayer males 9.07 ± 0.5 cm, albino slayer females 8.96 ± 0.4 cm, albino non slayer males 8.87 ± 0.17 cm, and albino non slayer female 8.86 ± 0.16 cm. Slayer phenotype category was identified based on the caudal length fin with 4.43 ± 0.64 cm. Parameters determined included the phenotype distribution of albino slayer, total number of eggs, fertilization rate, hatching rate and survival rate. The results showed that all crossing schemes produced four phenotypes classes including normal slayer, normal non slayer, albino slayer and albino non slayer. The slayer phenotype of albino brushmouth was highest percentage (75%) in crossing with male albino slayer (SN, SS) and highest number of eggs, while the reciprocal crossing with female albino slayer (NS) produced albino slayer 55% and lower egg count. The ratio of tail fin length to body length in albino slayer phenotype was lower than normal phenotype of broom fish. The fertilization and hatching rates were not significantly different in all crosses; however the number of eggs decreased in the second spawning. The similar phenotype cross between albino slayer phenotypes (SS) resulted the best average of survival rate (92%). Abstrak Muncar merupakan salah satu sentra produksi hiu di Pulau Jawa dengan daerah tangkapan di perairan Selat Bali dan sekitarnya. Pendataan secara rutin terhadap hasil tangkapan hiu dan pari dilakukan sejak Bulan Mei 2018 hingga April 2019 di Pasar Ikan Brak dan Pelabuhan Perikanan Pantai (PPP) Muncar, Banyuwangi untuk mengetahui kom-posisi spesies dan distribusi ukuran hiu dan pari yang tertangkap dari perairan Selat Bali dan sekitarnya. Pengumpulan data dilakukan secara sensus dan hampir seluruh individu diidentifikasi sampai tingkat spesies serta diukur. Tercatat 3.551 individu hiu dan pari yang didaratkan di wilayah tersebut selama kurun waktu penelitian, yang terdiri atas 75 spesies dan 25 famili (49 spesies hiu dan 26 spesies pari). Spesies hiu yang paling umum ditangkap berasal dari famili Carcharhinidae, sedangkan kelompok pari didominasi oleh famili Dasyatidae. Tercatat ada 13 spesies hiu dan pari Apendiks II CITES yang ditangkap oleh nelayan Muncar, yaitu Carcharhinus falciformis, Alopias pelagicus, A. superciliosus, Isurus oxyrinchus, I. paucus, Sphyrna lewini, S. zygaena, Mobula mobular, M. tarapacana, M. thrustoni, Glaucostegus typus, Rhynchobatus australiae dan Rhina ancylostoma. Sebagian besar hiu dan pari yang ditangkap nelayan berada pada ukuran yuwana hingga remaja, yang belum matang kelamin atau sedang menuju dewasa.
PRODUCTION AND BIOACTIVITY POTENTIAL OF THREE RECOMBINANT GROWTH HORMONES OF FARMED FISH Alimuddin Alimuddin; Indra Lesmana; Agus Oman Sudrajat; Odang Carman; Irvan Faizal
Indonesian Aquaculture Journal Vol 5, No 1 (2010): (June 2010)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.826 KB) | DOI: 10.15578/iaj.5.1.2010.11-17

Abstract

This study was aimed to produce recombinant growth hormone (rGH) from giant grouper (Epinephelus lanceolatus), giant gouramy (Osphronemus gouramy) and common carp (Cyprinus carpio) and compare their bioactivity potential by means of inducing the growth hormone of juvenile Nile tilapia (Oreochromis niloticus) as the model. DNA fragment encoding mature GH protein of giant grouper (El-mGH), giant gouramy (Og-mGH) and common carp (Cc-mGH) was amplified by PCR method. The purified PCR products were ligated to pCold-1 to generate pCold/El-mGH, pCold/OgmGH, and pCold/Cc-mGH protein expression vector, respectively. Each of the expression vectors was transformed into the Escherichia coli BL21. E. coli BL21 was cultured using 2xYT medium and protein production was induced by cold shock at 15±1oC for overnight. The inclusion bodies of E. coli transformants containing protein expression vector were isolated by sonication method, and rGH production was analyzed by SDS-PAGE. Juvenile of Nile tilapia of average body weight of 12.41±3.28 g was intramuscularly injected once a week for 4 weeks with 1 μg inclusion body containing rGH per gram fish body weight. The result showed that rGH in molecular weight of about 25 kDa was obtained. Fish injected with rGH of El-mGH, Cc-mGH and Og-mGH grew 20.94%, 18.09%, and 16.99% faster, respectively, compared with the control. This result indicated that the three rGH produced in E. coli possessed biological activity when tested on Nile tilapia and further research is needed to find its effect on the growth of other aquaculture fish species.
Co-Authors ', Alimuddin , Alimuddin . Alimuddin . Alimuddin . Syafiuddin Abinawanto Abinawanto Agus Oman Sudrajat Ahmad Teduh Alfis Syahril Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Amalia Hayati Anang Hari Kristanto Andi Aliah Hidayani ANNA OCTAVERA Aulia Saputra Aulia Saputra Ayi Santika Chairul Muluk D.S. Sjafei Dede Hermawan DEDI JUSADI Dendi Hidayatullah Deni Radona Deni Radona Deni Radona Dian R Herdianto Didik Ariyanto Didik Ariyanto Dinar Tri Soelistyowati Dita Puji Laksana Djamhuriyah S. Said Eddy Supriyono Enang Harris Eni Kusrini Ermianus Samalei Estu Nugroho Fajar Maulana . Fauzan Wahib Alsani Firda Amalia Sukma Flandrianto S. Palimirmo GORO YOSHIZAKI Goro Yoshizaki Goro Yoshizaki Handika Gilang Pramana Putra Hanung Adi Nugroho Harton Arfah Hasan Nasrullah Herjayanto, Muh. Hidayat Hidayat Hylda Khairah Putri I. Andriani I. Herviani Ibrahim Satrio Faqih Iis Diatin Indra Lesmana Irin Iriana Kusmini IRMA ANDRIYANI Irvan Faizal Irvan Faizal Irvan Faizal Irwan Irwan Jasmadi Jasmadi Jean-Christophe Avarre K. Sumantadinata Kadarusman Kadarusman Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Komar Sumantadinata Kukuh Adiyana Laurent Pouyaud Lies Emmawati Hadie Lies Emmawati Hadie Lina Mulyani Lola Irma Purwanti M. Syaifudin M. Syukur M. Zairin Junior Marlina Achmad Maskur Maskur Media Fitri Isma Nugraha MH. Fariduddin Ath-thar Mia Setiawati Moh. Abduh Nurhidayat Mubinun Mubinun Muh. Herjayanto Muhamad Fathurrizki Darmawan Muhamad Syukur MUHAMMAD AGUS SUPRAYUDI Muhammad Muhammad Muhammad Sami Daryanto Muhammad Zairin Muhammad Zairin Muhammad Zairin Jr Muhammad Zairin Jr. Muhammad Zairin Jr. N. Sugiri Nadia Mega Aryani Novi Megawati Nunun Ainun Putri Sari Banun Kaliky Nur Bambang Priyo Utomo Nurfitriani Siti Yumaidawati Prama Hartami Rahma Vida Anandasari Rahman Rahman Rahman Rahman Rahmat Hidayat Ratu Siti Aliah RIDWAN AFFANDI Rodhi Firmansyah Ronny Rachman Noor Rudhy Gustiano Rudhy Gustiano S. Purwati Siti Subaidah Siti Subaidah Sri Nuryati Sri Setyo Wulandari Sri Sundari Subandriyo Subandriyo Sukenda Sukenda Sukenda Sukenda Sumie Etoh Tatak Dwi Cahyono Toshio Takeuchi Tristiana Yuniarti Wartono Hadie Wartono Hadie Wedaraningtyas Nugrahani Yogi Himawan Yuni Puji Hastuti Yusran Ibrahim