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All Journal Natural Science: Journal of Science and Technology JURNAL KIMIA SAINS DAN APLIKASI Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Majalah Obat Tradisional BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Proceeding Biology Education Conference

Chairul Chairul

Pusat Penelitian Biologi LIPI, Jl. Ir. H. Djuanda No. 18, Bogor

Author-ID : 246272

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Engineering Health Professions Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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PENAPISAN AKTIVITAS ANTIOKSIDAN DAN BEBERAPA TUMBUHAN OBAT INDONESIA MENGGUNAKAN RADIKAL 2,2-DIPHENYL-1 PICRYLHYDRAZYL (DPPH) Wulansari, Dewi; Chairul, Chairul
Majalah Obat Tradisional Vol 16, No 1 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Antioksidan merupakan senyawa yang berguna mengatasi kerusakan oksidatif akibat radikal bebas dalam tubuh sehingga turut berperan mencegah berbagai macam penyakit. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan dari beberapa tumbuhan obat Indonesia. Sebanyak 37 ekstrak metanol tumbuhan obat diuji aktivitas antioksidannya secara in vitro menggunakan metode peredaman radikal bebas 2,2-diphenyl-1-picrylhydrazyl (DPPH) dan vitamin C sebagai kontrol positif. Hasil pengujian menunjukkan 29 ekstrak memiliki aktivitas diatas 50% pada konsentrasi 1000 ppm. 5 ekstrak dengan aktivitas tertinggi antara lain ekstrak kulit batang Sapium baccatum dan Leucosyke capitellata, ekstrak daun Ardisia crispa, Glochidion cauliflorum, dan Glochidion superbum.

Potensi Sifat Antioksidan pada 10 Jenis Ekstrak dari Famili Rubiaceae Marusin, Sofnie; Saefudin, Saefudin; Chairul, Chairul
JURNAL BIOLOGI INDONESIA Vol 9, No 1 (2013): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Famili Rubiaceae telah lama dikenal sebagai sumber tanaman obat Indonesia. Beberapa spesies seperti Coffea arabica,Morinda citrifolia, Chinchona succirubra telah digunakan sebagai obat rakyat sejak jaman dulu. Penelitian ini bertujuanuntuk mengetahui nilai peroksida dan aktivitas antioksidan pada 10 jenis ekstrak famili Rubiaceae yang lain.Nilai peroksida (POV) diukur menggunakan metode iodometri dan hasil pengukuran menunjukkan POV yang berbedanyata kulit kayu Anthocephalus macrophyllus (69,48), kulit kayu Wendlandia glabrata (67,86), kulit kayu Guettardaspeciosa (73,17) dan daun Paederia foetida (89,14). Aktivitas antioksidan diuji menggunakan metode tiosianat(FTC) pada empat jenis ekstrak yang berpotensi, yaitu kulit kayu A. macrophyllus, kulit kayu W. glabrata, kulit kayuG. speciosa dan daun P. foetida. Nilai absorbansi (A) dari masing-masing jenis adalah 0,294; 0,293; 0,365; dan 0,375,dibandingkan dengan kontrol negatif (0,919) dan kontrol positif (0,31).Kata kunci: Rubiaceae, sifat antioksidan, POV, metode tiosianat (FTC)

Pembuatan Karbon Aktif Dari Kulit Durian Sebagai Adsorbent Logam Fe Febriansyah, Beni; Chairul, Chairul; Yenti, Silvia Reni
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Durian peel is known as the waste that can pollute the environment. One effort to improve the economic value of the durian skin can be done with the process into activated carbon. This study aims to obtain optimum conditions for the use of activators of Kaliaum Hidroksida on the quality of activated carbon and activated carbon absorption of Fe metal. Activated carbon is made via two processes, namely the process of carbonization and activation at 320oC temperature with a solution of Kalium Hidroksida by varying the massa aktif carbon1, 1.5, 2, 2.5, dan 3 gr and adsorb time30, 60, 90, dan 120 menit. To determine the quality of activated carbon, activated carbon characteristics tested, such as moisture content, ash content, and the absorption of iodine. The results showed that the characteristics of activated charcoal SNI 06-3730-1995 meets the standards the water content of 14.12%, 5.46% ash content and absorption of I2 solution of 580.27 mg / g in adsorbing metals Fe. Application of Fe metal adsorption by activated carbon from durian skin with 3 grams of carbon mass variation and contact time of 90 minutes where the adsorption efficiency is 96.75%.Keywords: activated carbon, durian skin, potassium hydroxide

Pembuatan Bioetanol Dari Kulit Buah Nanas Dengan Metode Solid State Fermentation (SSF) Dan Pemurnian Dengan Proses Distilasi-Adsorbsi Dengan Variasi Ratio Bioetanol:Adsorben Safitri, Nadira; Chairul, Chairul; Amraini, Said Zul
Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Vol 1, No 1 (2014): Wisuda Februari Tahun 2014
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains

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Bioethanol is an ethanol which can be obtained by glucose fermentation from carbohydrate source that uses microorganisms help continued by distillation process. Adsorption is a separation process where the components of a fluid phase moves to the absorbing surface of the solid (adsorbent). In the fermentation process pineapple peel waste with Zymomonas mobilis and distillation process obtained the highest ethanol content of 11%, ethanol content obtained is not too high, so it needs a process that can increase the purity of bioethanol with distillation adsorption process. Bioethanol purification process using natural zeolite as adsorbent with variable change is the type of adsorbent of natural zeolite without activation with base activated natural zeolite and bioethanol:adsorbent ratio is 1:0,35 ; 1:0,5 ; 1:0,65. From the experimental results indicated that the highest ethanol content obtained of the zeolite and ethanol massa ratio of 1:0,65 with using base activated natural zeolite, ethanol content of 99,8% which fulfill Indonesian National Standard (SNI) for both technical ethanol and denatured bioethanol specification. Keywords : bioethanol, solid state fermentation, pineapple peel, distillation adsorption, natural zeolite

METIL EUGENOL, KHEMOTIPE DARIMINYAK ATSIRI Melaleuca spp. (MYRTACEAE) YANG TUMBUH DIKEBUN RAYA CIBODAS Murningsih, Tri; Chairul, Chairul; Kuncari, Emma Sri
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The essential oils of the fresh leaves of Melaleuca spp. (M. alternifolia Cheel, M. ericifolia Sm., M. squarrosa Donn, and M.genistifolia Sm.) from Cibodas Botanical Garden, Indonesia were isolated by a hydrodistillation method and analysed by a gas chromatography/mass spectrometry (GC/MS) technique. The various Melaleuca species showed differences within their relative essential oil compositions. The oils from all samples were of the methyl eugenol (10.30-71.25%) chemotype. The others major constituent were terpinen-4-ol (38.49%), linalool (19.65%) and bis(2-ethylhexyl)phtalate (66.09%) in the M. alternifolia, M. ericifolia, and M. genistifolia respectively.

ISOLASI GLUKOMANAN DARI DUA JENIS ARACEAE: TALAS {Colocasia esculenta (L.) Schott} DAN Iles-iles (Amorphophallus campanulatus Blumei) Chairul, Chairul; Chairul, Sofnie M
BERITA BIOLOGI Vol 8, No 3 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Talas {Colocasia esculenta (L.) Schott} and Iles-iles (Amorphophalus campanulatus Blumei) have been used as source of carbohydrate especially in tropical regions, mainly in East Indonesia. Talas and iles-iles, that contains carbohydrate and several substances for instance glucomannan would form viscous liquid and were used as emulgator, capsule cosmetics and dietary.The research was conducted to separate glucomannan from starch of talas and iles-iles fresh and dry (powder).The methods base on speed of revolution (centrifuge) in 5000 to 19000 rpm and the duration of rotation was 10 to 40 minutes and the filtrate was precipitated by using several alcohols (methanol, ethanol, propanol, isopropanol and isobutanol). The results showed that speed of rotation 17 x 1000 rpm with 10 minutes rotation resulted in good glucomannan separation from starch.Ethanol and isopropanol were the best solvent to separate glucomanan from dissolved starch in the filtrate with ratio 2 : 3 and for dry material, and the maximal agitation reached after 2 hours. Glucomannan content on fresh material was higher than as in talas (4.08 %) and iles-iles (5.64 %), compared to these in powder (dried) of talas (3.87 %) and iles-iles (5.41 %). The purerity of isolated glucomannan was higher compared to control.

PERBANDINGAN KOMPONEN KIMIA PENYUSUN MINYAK ATSIRI SIRIH LIAR {Piper ornatum) YANG BERASAL DARI SULAWESI SELATAN DAN PULAU SERAM DENGAN SIRIH BIAS A (Piper betle) Sulianti, Sri Budi; Chairul, Chairul
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1223

Comparison study on the chemical components of P. ornatum (Piperaceae) volatile oil, collected from Enrekang (South Celebes)and Sepa Village (Ceram Island, Moluccas) had been carried out by water distillation and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Those plants had exotic performance. The sample from South Celebes had original red color leaves and strong aromatic smell, but one from Ceram Island has green color leaves and weak aromatic smell. The results showed that volatile oil content of P. ornatum from Enrekang has higher than Sepa Village (0.94% and 0.81%), while the volatile oil of common piper (P.betle) was around 0.9-1.2%. GC-MS analysis determined that volatile oil of P. ornatum had 22 chemical components. Both volatile oil indicated similar peak fragmentation, in different intensities. The major component of P. betle and P. ornatum was caryophyllene i.e. 30.01% in the red leaf, 29.41% in the green leaf and 31.05% in P. betle. Some of minor components of P.ornatum were different, from P. betle.

Di Sela-Sela Laboratorium dan Plot Eksperimen MENGENAL HPLC: PERANANNYA DALAM ANALISA DAN PROSES ISOLASI BAHAN KIMIA ALAM Murningsih, Tri; Chairul, Chairul
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The development of method to explore natural chemical product initially from plants were started from the beginning and generously from time to time from the conventional ones until sophisticated equipments.The value of plants as food,cosmetic and medicines resources needs specific instruments and methods for determining the quantity as well as quality of the materials in plants.This paper describe in detail one of the sophisticated equipments commonly used for analyzing and isolating bioactive compounds the so called High Performance Liquid Chromatography (HPLC).

EFEK HYPOGLIKEMIK EKSTRAK ALKOHOL HERBA MENIRAN (Phyllanthus niruri L.) PADA KELINCI PUTIH JANTAN Chairul, Chairul; Jamal, Yuliasri; Zainul, Zuherti
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A study on the influences of alcohol extract of Meniran or Phyllanthus niruri L. (Euphorbiaceae) to blood glucose concentration in rabbits (Oxytolagus cuniculus) had been carried out using glucose tolerance method.The extract was administered orally at 10, 20, 30, 40 and 50 mg/kg BW.Prior to tfie extract administration, all rabbits were put on hyperglycemic condition by injecting glucose solution intravenally at dose of 0.5 g/kg BW.The blood glucose concentration was measured every one hour starting at 2 hours before and until 7 hours after administration. Antidiabetic drug,tolbutamid 250 mg/kgBW.was used as control.The results indicated that extract with dose at 30 mg/kg BW gave a hypoglycemic effect similar to control (Tolbutamid). The blood glucose decreased regularly as the extract dose being higher.Using statistical analyses showed significant differences among the groups, especially after the 2 and 3 hours of treatment (P<0.05).

PENGARUH PEMBERIAN EKSTRAK ALKOHOL AKAR ILALANG {Imperata cylindrica L.) TERHADAP PENURUNAN SUHU TUBUH TIKUS PUTIH JANTAN Chairul, Chairul
BERITA BIOLOGI Vol 5, No 2 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Antipyretic effect of alcohol extract ofilalang flmperata cylindrica L) root on white rat males had been studied using fever tolerance method.Experimental animals were induced to fever by injecting 0.9 ml/kg BW vaccine kotipa orally.Alcohol extract 5, 10, 30, 50,100 and 150 mg/kg BW was given one hour after injection.The body temperature was measured before injection and every half an hour until the fifth hour after injection.Paracetamol 200 mg/kg BW was used as control.Data were analyzed using ANOVA with the body temperature (T), the time interval (P) and extract doses being given (C) as parameters.The results showed that body temperature increased TC from the basal temperature at the third hour after injection of vaccine.The body temperature did not change significantly at the lower extract doses(5-30 mg/kg BW)(P>0.05),while higher doses (30-150mg/kg BW) linearly lowered the body temperature significantly (P<0.05).The extract dose at 50 mg/kg BW lowered the body temperature similar to paracetamol 200 mg/kg BW.

Co-Authors ANDRIA AGUSTA Astrandana, Yudi Dewi Kusrini DEWI WULANSARI Dirgantara, Doni Ari Drastinawati Drastinawati, Drastinawati Emma Sri Kuncari Febriansyah, Beni Hendra, Alfino Izmiarti Izmiarti, Izmiarti Khoirul Amru Nst, Khoirul Amru Maria Peratenta S, Maria Peratenta Maria Peratenta Sembiring Marpaung, Masdalena Muh Wawan Junuansyah, Muh Wawan Muhammad Furqan, Muhammad Nuryanti, Lidya Rahmah, Yulia Rohaya, Rohaya Saefudin Saefudin Safitri, Nadira Said Zul Amraini Serly Marselina Arifin, Serly Marselina Silaban, Kardiman Silvia Reni Yenti Sitinjak, Jufri Suanto Sofnie M Chairul Sofnie Marusin Soleh Kosela Solfiyeni Solfiyeni Sri Budi Sulianti, Sri Budi Sri Rezeki Muria SYAIFUL BAHRI Syelvia Putri Utami Tri Murningsih, Tri Triani, Leni Villarul, Trya Nungky Wahyu Hidayat Wahyu, Pri Widodo, Prio Wulandari, Zarah Ayu Yasminto, Habib Maulana Yaumil Mutia Akhir, Yaumil Mutia Yelmida Azis Yuanita, Mira Yuliana Yuliana Yuliasri Jamal Zainul, Zuherti Zultiniar, Zultiniar

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