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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences AJIE (Asian Journal of Innovation and Entrepreneurship) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Akuatik: Jurnal Sumberdaya Perairan Jurnal Perikanan Universitas Gadjah Mada COJ (Coastal and Ocean Journal)

Asadatun Abdullah

Departemen Teknologi Hasil Perairan, Fakultas Perikanan Dan Ilmu Kelautan, Institut Pertanian Bogor, Bogor

Author-ID : 2465233

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Library & Information Science Social Sciences

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ISOLASI DAN IDENTIFIKASI MIKROBA SIMBION SPONGE Axinella sp. Asadatun Abdullah
Jurnal Ilmu Pertanian Indonesia Vol. 11 No. 3 (2006): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Sponges are host organisms for various symbiotic microorganisms. Various symbiotic microorganisms have been found in Sponges such as archaea, heterotrophic bacteria, cyanobacteria, green algae, red algae, cryptophytes, dinoflagellates, and diatoms. The objectives of the research were to isolate and identify teh Axinella sp. Sponge-symbiotic microoganisms such asbacteria, micro fungi, and yeast. Sponge-symbiotic microoganisms that have been isolated cosisted of 7 bacteria isolates, 3 micro fungi, and 2 yeast isolates. Result from this research showed that the genus of bacteria was Alteromonas, Bacillus, and 2 yeast isolates have not been to identified.

KARAKTERISTIK KIMIAWI DARI DAGING KERANG TAHU, KERANG SALJU DAN KEONG MACAN Asadatun Abdullah; Nurjanah Nurjanah; Taufik Hidayat; Rizky Chairunisah
Jurnal Teknologi dan Industri Pangan Vol. 28 No. 1 (2017): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Meretrix meretrix, Pholas dactylus and Babylonia spirata are potential fisheries commodity, but information about their nutritional values is still limited. This research was aimed to determine the chemical compositions of Meretrix meretrix, Pholas dactylus and Babylonia spirata. Proximate of Meretrix meretrix, Pholas dactylus and Babylonia spirata were analyzed using thermogravimetric, soxhlet and kjeldahl methods. Their amino acid composition and taurine content were analyzed using High Performance Liquid Chromatografy (HPLC). The moisture, ash, fat, protein and carbohydrate contents of Meretrix meretrix were 79.98%, 1.37 0.11 9.39 and 9.02 respectively; Pholas dactylus were 83.78%, 1.19, 0.11, 11.37 and 3.55 respectively; while Babylonia spirata 78.44, 1.20, 0.33, 17.38 and 2.65 respectively. Arginine was the highest essential amino acid found in Meretrix meretrix, Pholas dactylus and Babylonia spirata meat with values of 1.12%, 0.93 dan 2.50 respectively. Glutamic acid was the most abundant non-essential amino acid with the highest value of 2.24, 2.14 and 3.76. The limiting amino acid was histidine with the value of 0.25%, 0.18 and 0.34, respectively. The taurine content of Pholas dactylus meat (0.085%) was higher than the Babylonia spirata and Meretrix meretrix (0.067 and 0.062%, respectively).

Inhibitors Screening of β-lactamase Axinella Sponge Simbion Bacteria sp. Asadatun Abdullah; Linawati Hardjito; . Ernawati; Fatimah Abdillah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 11 No 2 (2008): Buletin Teknologi Hasil Perikanan
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Salah satu jenis antibiotik yang umum digunakan dalam pengobatan adalah β-laktam. Saat ini terdapat banyak bakteri patogen pada manusia yang resisten terhadap antibiotik β-laktam. Bakteri patogen yang resisten terhadap antibiotik β-laktam adalah bakteri yang mampu memproduksi enzim β-laktamase. Tujuan penelitian ini adalah menapis produk alami inhibitor β-laktamase dari mikroorganisme simbion Axinella sp. Berdasarkan penelitian yang dilakukan sebelumnya, telah diisolasi dan diidentifikasi secara biokimia 7 isolat bakteri simbion sponge Axinella sp. Penapisan inhibitor β-laktamase dilakukan dengan metode agar difusi menggunakan bakteri target E.coli dan S.aureus resisten ampisilin koleksi Laboratorium Bioteknologi Hasil Perairan. Pengukuran resistensi terhadap E.coli dan S.aureus dilakukan pada konsentrasi ampisilin >32 μg/ml. Dari 7 isolat bakteri simbion yang ditapis secara intraseluler dan ekstraseluler, 4 isolat pada ekstraselulernya positif menghambat pertumbuhan bakteri patogen E.coli dan S.aureus. 4 bakteri simbion tersebut adalah Bacillus sp., Halomonas sp., Alteromonas sp. dan Alteromonas sp. Identifikasi molekuler bakteri simbion dan karakterisasi golongan senyawa inbitor β-laktamase sedang dalam proses penelitian.Keywords : bakteri simbion Axinella sp., inhibitor β-laktamase, E.coli, S.aureus

Physical and Chemical Characteristics of Agar Bacto with Addition Of Chitosan Asadatun Abdullah; Pipih Suptijah; Ruddy Suwandi
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 11 No 1 (2008): Buletin Teknologi Hasil Perikanan
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Agar adalah bentuk koloid dari suatu polisakarida kompleks yang diekstrak dari beberapa kelompok alga merah (Rhodophyceae). Salah satu pemanfaatan dari agar adalah sebagai media untuk pertumbuhan mikroba. Agar bakto adalah salah satu medium kultur yang digunakan untuk membantu menggelifikasi berbagai jenis larutan medium atau larutan kaldu (broth). Penambahan kitosan sebagai absorben pada proses pemurnian agar diharapkan dapat menghasilkan agar yang murni (refine agar) yang dapat digunakan untuk agar bakto sebagai media pertumbuhan mikroba. Tujuan dari penelitian ini adalah memodifikasi berbagai konsentrasi kitosan dan waktu proses absorbsi dalam pembuatan agar bakto sebagai media pertumbuhan mikroba. Penelitian dibagi menjadi dua tahap, yang pertama adalah tahapan proses isolasi kitin dan deasetilasi kitin menjadi kitosan dan penelitian utama yang dilakukan yaitu pembuatan agar bakto dengan menambahkan berbagai konsentrasi kitosan (0,5%, 1%, 1,5%) sebagai absorben terhadap agar yang dihasilkan dari Gracilaria sp. dan agar batang. Perlakuan kedua yang diberikan yaitu waktu proses absorbsi setelah dilakukan penambahan kitosan. Proses absorbsi yang dipilih 0 menit, 15 menit, 30 menit, dan 45 menit. Kombinasi perlakuan paling optimum untuk agar bakto berbahan baku Gracilaria sp. adalah pada konsentrasi kitosan 1% dengan lama pemanasan lanjutan ekstraksi selama 45 menit. Kombinasi perlakuan paling optimum untuk agar bakto berbahan baku agar batang adalah pada konsentrasi kitosan 0,5% dengan tanpa pemanasan lanjutan.Kata kunci: agar bakto, Gracilaria sp. , kitosan

Karakteristik Fisik dan Kimia Tepung Cangkang Kijing Lokal (Pilsbryoconcha exilis) Asadatun Abdullah; Nurjanah nurjanah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 13 No 1 (2010): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Local mussel (Pilsbryoconcha exilis) is one aquatic commodity that has high enough potential. Mussel shell is solid waste that has not been used optimally. The purpose of this research is to study the physical and chemical characteristics shells and mussel shell flour with the different of size and body length. The observed parameters include the physical characteristics of shells, yield, chitin, flour yield, degree of white, proximate contents, pH, minerals and mineral solubility determination of mussel shell flour. Mussel obtained from waters Situ Gede has length between 72-103 mm, 31-47 mm high and 13-34 mm thick. Mussel shells in all size contained chitin ranging from 0,72% to 0,75%. Mussel shell flour measuring < 90 mm 20% larger than the size of mussel ≥ 90 mm. Mussel shell flour measuring < 90 mm has a value of 5% degrees whiter than white shell size ≥ 90 mm. Shell flour has a water content between 1,19-1,2%, 93,14-93,34% of ash, 1,85-2,31% of protein, 0,66-0,72% of fat, carbohydrate by difference 2,62-2,94% with a pH range of 8,5-8,9. Mussel shell flour has a mineral content, respectively from the largest which are calcium, phosphorus and magnesium. The calcium and phosphorus mussels flour shell has an optimal value for solubility at pH 2. Mussel shells contained chitin ranged from 0,72% to 0,75%. Mussel shell size difference gave a significantly different effect on the physical characteristics of the mussel shell flour produced but did not influence significantly different to the chemical characteristics of the mussel shell flour produced.

AUTENTIKASI TUNA STEAK KOMERSIAL DENGAN METODE PCR-SEQUENCING Asadatun Abdullah; . Nurjanah; Nanang Kurnia
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 14 No 1 (2011): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Tuna is one of the  shery commodities which are susceptible to mislabeling and substituted with similarspecies, but lower price. Consumer as a purchaser will incur a loss (economical fraud) so it is needed a way toovercome these problems. This study aimed to optimized extraction of DNA obtained from the tuna and tunaexporter companies of modern markets, identi cation of DNA-based species-speci c primers with a target genecyt b, and characterization of DNA tuna authentication results. This study consisted of several steps beginning with the characterization of tuna, DNA extraction using CTAB method and Vivantis kit, ampli cation by PCR, electrophoresis, and nucleotide sequencing. The samples tested were successfully extracted and ampli ed with the appropriate size of 750 base pairs. PCR sequencing using cyt b gene targets resulted in the identi cation of tuna raw material. PCR sequencing of the nucleotide sequence of results which have been  tted to the NCBI data, which does not show any fraud in the form of substitution with other species. Species of yellow  n (Thunnus albacore), Albacore (Thunnus alalunga), big eye (Thunnus obesus) and blue  n (Thunnus macoyyi) has the highest homology i.e 99%, 99%, 99%, 100%, respectively.Keywords: authentication, cyt b, mt-DNA, PCR, tuna’s steaks

AKTIVITAS ANTIOKSIDAN DAN KOMPONEN BIOAKTIF KEONG IPONG-IPONG (Fasciolaria salmo) . Nurjanah; Asadatun Abdullah; Azwin Apriandi
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 14 No 1 (2011): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Ipong-ipong snail (Fasciolaria salmo) is one of sea water gastropods that have not been utilized optimally. People believe that the snail has the ef cacies for increasing stamina and vitality. However, scienti c data supporting the ef cacy of this snail has not been elucidated yet. The purposes of this research was determined the yield, chemical content (water, protein, fat, ash, acid insoluble ash and carbohydrates), bioactive components and antioxidant activity. The yield of shells, meat, and viscera were 69.69%, 22.08% and 8.22%, respectively. Watercontent, protein, fat, ash, acid insoluble ash and carbohydrates were 73.075%, 18.28%, 0.575%, 2.77%, 0.15% and 5.2%, respecitevely. Six bioactive components were detected i.e alkaloids, steroids, reducing sugars, carbohydrates, peptides and amino acids. Antioxidant activity using DPPH method obtained the result IC50 of the meat extract with chloroform, ethyl acetate and methanol solvent were 9210 ppm, 6825 ppm, 1513.8 ppm, respectively. Meanwhile for viscera extract were. 2825 ppm, 4600 ppm and 994,47 ppm, respectively.Keywords: antioxidants, bioactives, Fasciolaria salmo, proximate, yield.

Identification and Profiling of Active Compounds from Golden Apple Snail’s Egg Pigments Asadatun Abdullah; N. Nurjanah; Muhammad Reyhan
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20 No 2 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Golden apple snail (Pomacea canaliculata) has been known as rice corps pest due to high adaptability and reproductive power. Utilization of Pomacea canaliculata’s eggs as raw materials in the food and health industry is one of the efforts to eradicate the pest snail. This study was aimed to identify the active compounds contained in the extract pigments of Pomacea canaliculata’s eggs. The methods of this study were extraction of pigments using acetone and methanol, analyzing the active compound (secondary metabolite) qualitatively, TLC to determine pigment components and LC-MS/MS to identify active compounds semi quantitatively. The results showed that active compounds in the methanol extract contain 11 carotenoid pigments of xanthophyl group, two carotenoid pigments of carotene group, and 2 active compounds in nonpigmented form, whereas the acetone extract contain 11 pigmentcarotenoids of xanthophyl group and 2 compounds active in non-pigment form.

Antioxidant Activities of Various Brown Seaweeds from Seribu Islands Seftylia Diachanty; N. Nurjanah; Asadatun Abdullah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20 No 2 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Brown seaweeds contain various metabolites (carotenoids, laminarin, alginate, fucoidan, mannitol, and phlorotannin) as anticancer substances, antioxidants and chemopreventive agent against degenerative diseases. This research was aimed to determine the chemical properties and antioxidants activity of Sargassum polycystum, Padina minor and Turbinaria conoides. This research consisted of the proximate analysis, crude fiber, minerals, heavy metals, extraction, phytochemicals, analysis phenolic compound and antioxidant activity with DPPH, FRAP and CUPRAC methods. The results showed moisture content of S. polycystum, P. minor, and T. conoides 24-31%, fat 0.23-0,47%, protein 3-4%, ash 27-45%, carbohydrate 26-38% and crude fiber 2-6%. Mineral Fe S. polycystum, P.minor and T.conoides about 0.03 g/kg to 0.39 g/kg, Ca 11.28 g/kg to 27.98 g/kg, K 13.86 g/kg to 41.15 g/kg, Na 16.31 g/kg to 20.51 g/kg and Mg 8.04 g/ kg to 18.68 g/kg. The range of heavy metals Pb S. polycystum, P. m i n o r and T. conoides 3.48 mg/kg to 12.68 mg/kg, Hg 0.24 mg/kg to 0.53 mg/kg and Cu 5.66 mg/kg to 7.02 mg/kg. Ethanol extract of S. polycystum, P. minor, and T. conoides contains alkaloids, phenolic, flavonoids, saponins, steroids, and triterpenoids. Total phenolic compounds of brown seaweeds ranged from 3758.97-8287.18 mg GAE/g. Brown seaweeds contain DPPH antioxidant activities within IC50 values of 1.9-9.6 mg/mL, FRAP 70.643-105.357 µmol Trolox/g and CUPRAC 85.268-201 µmol Trolox/g.

Characteristics of Green Seaweeds from Seribu Islands and Sekotong West Nusa Tenggara Antioxidant Chairun Nufus; Nurjanah Nurjanah; Asadatun Abdullah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 20 No 3 (2017): Jurnal Pengolahan Hasil Perikanan Indonesia 20(3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Green seaweeds contain many bioactive compounds which one of them act as antioxidants as wellas rich in fibers and essential minerals. This study was aimed to determine physicochemicals propertiesof green seaweed Caulerpa lentillifera, Halimeda opuntia and Ulva lactuca. The analitycal methods of thisresearch consists of: proximate analysis, mineral composition, phytochemical compounds, heavy metalcontent and antioxidant activity of extract green seaweed by CUPRAC, DPPH and FRAP method. Theproximate analysis showed that the water content of the three seagrasses ranged from 10.83-28.41%, the ashranged from 24.97-70.66%, the fat ranged from 3.80 to 5.26%, the fiber ranged from 2, 63-3.86%, proteinranges from 0.18 to 1.43% and carbohydrates range from 10.69 to 36.49%. Seaweeds contain Ca mineralsranging from 17.95-124.39 g / kg, Na ranges from 21.16 to 34.18 g / kg. Mg ranges from 2.63-22.23 g / kg,K ranges from 2.29-12.28 g / kg and Fe ranges from 0.13-0.34 g / kg. The three seaweeds contain Pb heavymetals ranging from 13.57-37.76 mg / kg, Cu ranges from 5.02-8.32 mg / kg and Hg ranges from 0.170 to0.265.. bioactive compound extract C. lentillifera, H. opuntia and U. lactuca contains alkaloids, flavonoids,phenols hydroquinone, saponins, steroids and triterpenoids. Green seaweeds contain DPPH antioxidantactivity within IC50 values of 43,53-95,91 mg/L, CUPRAC 138.21–212.43 μmol trolox /g, FRAP 175.93–181.79 μmol trolox/g.

Co-Authors . Ernawati . Nurjanah . Nurjanah Ade Irma Suryani Nasution Agoes M. Jacoeb Agoes M.Jacoeb Agoes Mardiono Jacoeb Anggrei Viona Seulalae Anggrei Viona Seulalae Aninditya Artina Setiaputri Anita Nurul Firdaos Ari Elisa Ratih Aulia Azka Aulia Sekar Sari Azwin Apriandi Chairun Nufus Chairun Nufus Chandabalo Chandabalo Evi Maya Sari Evi Maya Sari Fatimah Abdillah Giri Rohmad Barokah Hana Aulia Sativa Hanifah Husein Hizbullah Ikromatun Nafsiyah Kinanti Permata Sitaresmi Laili Izzati LINAWATI HARDJITO M. Fedi A. Sondita Mala Nurilmala Misla Alfitri Muh. Alsere Bardian Sahaba Muhamad Fedi Alfiadi Sondita Muhammad Reyhan N. Nurjanah Nadia Fabella Nanang Kurnia Norita Norita Novia Nanda Saputri Nur Azizah Nasution Nurjanah Nurjanah Nurjanah - - Nurjanah - Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Nurjanah Pipih Suptijah Puji Rianti Rachmawati Rusydi Rahma Dini Arbajayanti Ramlan Raudhi Kurniawan Rizky Chairunisah Rizsa Mustika Pertiwi Rizsa Mustika Pertiwi Rizsa Pertiwi Rodieser Sembiring Roni Nugraha Roza Yusfiandayani Ruddy Suwandi Seftylia Diachanty Shabrina Aulia Sharah Novita Sari Shindy Hamidah Manteu Siti Fauziyah TATI NURHAYATI Taufik Hidayat Vicentius Marco Matutina

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