Dendi Hidayatullah
Departemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor

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Kinerja probiotik Bacillus sp. pada pendederan benih ikan lele Clarias sp. yang diinfeksi Aeromonas hydrophila , Sukenda; Muhammad Mufthi Rafsyanzani; , Rahman; Dendi Hidayatullah
Jurnal Akuakultur Indonesia Vol. 15 No. 2 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3230.037 KB) | DOI: 10.19027/jai.15.2.162-170

Abstract

ABSTRACT This experiment was conducted to assess performance of Bacillus sp. probiotic on catfish juvenile Clarias sp. infected by Aeromonas hydrophila. The probiotic content in the diets were 0% (K+ and K-), 1%, and 2% in duplicates. This experiment used randomized design with four treatments and two replications. Juveniles with average body weight of 3.22±0.15 g/fish were reared in the 1.5×2.8×0.5 m3 pond with density of 800 fish/pond. Fish were reared for 30 days and fed three times a day at rate 8% of  total body weight. At day 31, catfish were challenged by A. hydrophila 0.1 mL (106 cfu/mL). Post infection observation was carried out ten days with density 10 fish/aquaria. The result showed that fish fed diet containing 2% probiotic gave the best probiotic performance with survival rate of catfish 83.33% after challenged, spesific growth rate 5.40%, and 0,75 of feed conversion ratio. The results of the blood profile showed significantly better results in the treatment of probiotics compared to the positive control after challenge test A. hydrophila. Probiotic Bacillus sp. has given as much as 2% on feed provides better performance on catfish juvenile. Keywords: probiotic, Bacillus sp., A. hydrophila, catfish juvenille, growth  ABSTRAK Penelitian ini bertujuan untuk menguji kinerja probiotik Bacillus sp. dalam pakan pada pendederan benih ikan lele Clarias sp. yang diinfeksi bakteri Aeromonas hydrophila. Penelitian ini menggunakan rancangan acak lengkap dengan empat perlakuan yaitu kandungan probiotik dalam pakan perlakuan yaitu 0% (K+ dan K-), 1%,  dan 2%, masing-masing dengan dua ulangan. Ikan lele yang digunakan memiliki bobot rata-rata 3,22±0,15 g/ekor, dipelihara dalam kolam terpal berukuran 1,5×2,8×0,5 m3 dengan kepadatan 800 ekor/kolam. Ikan dipelihara selama 30 hari dengan frekuensi pemberian pakan tiga kali sehari sebanyak 8% dari bobot tubuh ikan. Hari ke-31 benih lele diinjeksi bakteri A. hydrophila dosis 0,1 mL/ekor dengan kepadatan bakteri 106 cfu/mL. Pemeliharaan setelah diinfeksi dilakukan selama sepuluh hari dengan kepadatan 10 ekor/akuarium. Hasil penelitian menunjukkan ikan yang diberi probiotik 2% memperlihatkan kinerja probiotik terbaik dengan tingkat kelangsungan hidup ikan lele sebesar 83,33% setelah diinfeksi dengan A. hydrophila; laju pertumbuhan harian sebesar 5,40%; dan konversi pakan 0,75. Hasil gambaran darah menunjukkan hasil yang signifikan lebih baik pada perlakuan pemberian probiotik dibandingkan kontrol positif pascauji tantang A. hydrophila. Probiotik Bacillus sp. yang diberikan sebanyak 2% pada pakan memberikan kinerja lebih baik pada pendederan benih ikan lele. Kata kunci: probiotik, Bacillus sp., A. hydrophila, benih lele, pertumbuhan 
Specific Immune Response Kinetics and Mortality Patterns of Tilapia Oreochromis niloticus on Post-Cocktail Vaccination Period against the Infection of Aeromonas hydrophila and Streptococcus agalactiae Sukenda Sukenda; Tuti Sumiati; Sri Nuryati; Angela Mariana Lusiastuti; Dendi Hidayatullah
Journal Omni-Akuatika Vol 13, No 2 (2017): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.583 KB) | DOI: 10.20884/1.oa.2017.13.2.279

Abstract

Fish vaccination aims to induce a specific immune response indicated by an increase of antibodies in vaccinated fish. However, in accordance with time the presence of will continue to decline. The purpose of this study was to determine the kinetics of specifik immune response and trend mortality against Aeromonas hydrophila and Streptococcus agalactiae on tilapia following vaccination with cocktail vaccine. Fish vaccinated through immersion for 30 minutes in a solution of diluted vaccine. Challenge test was performed for three periods, on day 22, 50, and 78 post-vaccination, fish were challenged with single infection of A. hydrophila 108 cfu. mL-1 and S. agalactiae 104 cfu. mL-1 and co-infection of both bacteria by intraperitoneal. During rearing, the blood fish were taken for determining of serum antibodies, and its  measured by ELISA. The results showed that the concentration of specific antibodies vaccinated fish were significantly higher than the control. The basal antibody levels of A. hydrophila before vaccination were higher than S. agalactiae with OD of 0.104 and 0.069 respectively. The maximum  antibody  response  was  reached  within  70  days  of  the  A. hydrophila OD= 0.264 and 56 days against S. agalactiae OD= 0.188. The mortality rate in the control group was significantly higher than vaccinated on all types and each challenge test period. The trend of mortality due to a single infection of A. hydrophila and co-infections occur more quickly than by S. agalactiae. Lowest mortality occurred in the vaccinated group at 50 day tested challenge.Keywords: kinetics antibody, Aeromnas hydrophila, Streptococcus agalactiae, Oreochromis niloticus