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Studi Biologi Kutu Sisik Lepidosaphes beckii N. (Homoptera: Diaspididae) Hama pada Tanaman Jeruk Otto Endarto; Susi Wuryantini
Jurnal Hortikultura Indonesia Vol. 4 No. 2 (2013): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

ABSTRACTThe objective of this research was to determine life -cycle, morphology of each development stage, including form, color , size, and fecundity of L. beckii. This research was carried out in March until August 2006 at Entomology Laboratory, Indonesian Citrus and Subtropical Fruits Research Institute, Tlekung, Batu. The method of this research included observation of life- cycle of L. beckii, morphology and fecundity. The result of larvae stages period, prepupa, pupa and adult showed that L. beckii have average life-cycle 31.2 ± 1.4 days with temperatures ranged 26 – 35 0C. Egg of L. beckii was oval with both dull tip, colored white, the average length 0.20 ± 0.05 mm and average width 0.16 ± 0.04 mm, the average amount of eggs produced was 78.7 ± 2.7. Clowler colored white yellowish t hin oval, with average length of 0.21 ± 0.05 mm and width 0.12 ± 0.04 mm. Shield white cap have average length size of 0.64 ± 0.71 mm and shield width 0.35 ± 0.11 mm. Shield on instars 2 colored nutbrown and then deep brown, shield have length size 1.37 ± 0.15 mm and width 0.36 ± 0.08 mm. Pre- pupa have body length of 1.67 ± 0.18 mm and width of 0.39 ± 0.08 mm, whereas pupa have shield length of 2.01 ± 0.2 mm and width of 0.56 ± 0.10 mm. Female adult have average length of shield 2.09 ± 0.21 mm and width 0.79 ± 0.12 mm.Key words : citrus, fecundity, life- cycle, morphology, pestABSTRAKPenelitian bertujuan untuk mengetahui siklus hidup, morfologi setiap tahap perkembangan, L. beckiiyang meliputi bentuk, warna dan ukuran dan daya tetas telur. Penelitian dilaksanakan pada bulan Maret -Agustus 2009 di Laboratorium Entomologi Balai Penelitian Tanaman Jeruk dan Buah Subtropika Tlekung Batu. Penelitian ini dilakukan dengan metode deskriptif, pengamatan dilakukan dengan mengamati langsung perkembangan setiap stadia L. beckii. Hasil pengamatan terhadap lama stadia larva, prepupa, pupa dan imago menunjukkan L. beckii mempunyai siklus hidup rata- rata 31. 2±1.4 hari dengan suhu 26- 350C. Telur L. beckii, berbentuk oval dengan kedua ujungnya tumpul dan berwarna putih ukuran telur panjang rata- rata 0.20±0.05 mm dan lebar rata- rata 0.16±0.04 mm Daya tetas telur sebesar 68.29% dengan rata- rata telur yang dihasilkan 78.7±2.7 butir. Crawlers berwarna putih kekuningan dan berbentuk oval pipih, panjang rata- rata 0.21±0.05 mm dan lebar rata- rata 0.12±0.04 mm. White cap berwarna putih dengan ujung yang runcing berwarna cokelat perisai w hite cap berukuran panjang rata- rata 0.64±0. 71 mm dan lebar ratarat a 0.35±0.11 mm. Instar 2 perisai berwarna cokelat muda lama kelamaan warnanya akan berwarna cokelattua, panjang perisai 1.17 ±0.15 mm dan lebar 0.36±0.08 mm. Pre- pupa berwarna cokelat panjang perisai 1.37±0.18 mm dan lebar 0.39±0.08 mm. Pupa berwarna cokelat dengan ujung yang tumpul b erwarna ungu, panjang perisai 1.67±0.18 mm dan lebar 0.56±0.10 mm, Imago betina berwarna cokel at, panjang perisai rata- rata 2.09±0.21 mm dan lebar rata- rata 0.79±0.12 mm.Kata kunci: jeruk, kesuburan, siklus hidup, morfologi, hama

Sistematik Filogenetik Isolat-isolat Kapang Indigenous Indonesia sebagai Entomopatogen Kutu Sisik (Lepidoshapes beckii Newman) Hama Tanaman Jeruk Suharjono Suharjono; Agung P.W. Marhendra; Anang Triwiratno; Susi Wuryantini; Lina Oktavia R
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i2.2708

Citrus fruits constitute a basic agricultural product in many countries. They are a source ofnational income, empowering labor market in rural zones and those surrounding urban areas,and in addition to being an important part of the diet. Insects are common problems to plantingof citrus. Purple Scale, Lepidosaphes beckii (Newman) is one of the destructive insect pests ofcitrus worldwide. The control of purple scale insects in citrus utilizes native natural enemiesincluding pathogens. Most known species of mould are pathogens of purple scale insect of citrus.The objective of this research was to study phylogenetic systematics of Indonesian indigenousmould isolates as pathogen on purple scale insect (Lepidoshapes beckii Newman) pest of citrusplants. Seven mould isolates derived from Purworejo, Brastagi, and Poncokusumo were showedpathogenic on purple scale insect. The fungal isolates were cultured on PDA. The DNA of fungalmaterial was isolated with Kit Prepman Ultra (Applied Biosystems). Nuclear ribosomal LargeSubunit 28S rDNA (LSU) was amplified using primers ITS1 and ITS4. Products of PCR werepurified and sequenced. Based on the 28S rDNA sequence, the result was showed that isolate ofPW ASG D4 was related to Gibberella moniliformis dx-12, PC ORG D4 was related to Gibberella sp.1893, PW ASG D2 was related to Gibberella moniliformis BZ070101, PW LB D2 was related toGibberella moniliformis 52, BRS D22 was related to Fusarium oxysporum DC-1-67, PWRJ I D4 wasrelated to Fusarium culmorum SQ070108, and BRS D23 was related to Aschersonia sp. Ag-11.

Test of Various Carrier Materials Against Viability and Conidia Density in Some Liquid Biopesticides of Entomopathogenic Fungi Unun Triasih; dina agustina; mutia erti dwiastuti; susi wuryantini
JURNAL AGRONIDA Vol. 5 No. 1 (2019)
Publisher : Universitas Djuanda Bogor

Indonesia is a country that has a large biological diversity but has not been fully utilized for agriculture. The effectiveness of biological agents in controlling pests and plant diseases still needs further investigation, one of which is the use of entomopathogenic fungi as biopesticides. The purpose of this study was to determine the effect of storage on the viability and density of conidia in several entomopathogenic fungi; Beauveria bassiana, Metarhizium anisopliae, Paecilomyces. This study used 5 entomopathogenic fungi isolates, 2 Beauveria bassiana isolates (JBG and JBR), 2 Metarhizium anisopliae isolates (JBG and UST), 1 Paecilomyces. Biopesticide carrier material consisted of 6 carrier materials, namely soybean decoction waste, soy washing waste, Potato Dextrose Agar, tapioca waste, rice waste, rice washing waste and coconut water. Biopesticides have been made one year before and the viability and complexity of the conidia is re-examined one year later. From the observations of conidial density, the best was found in Beauveria bassiana JBG liquid biopesticide of 4.8 x 10 x conidia / ml from the initial density of 3.4 x 10⁸ / ml in the soybean decoction (RK) carrier material. The highest viability was found in JBG Metharizium anisopliae on soybean washing waste material by 14%. Key words: Conidia viability, conidial density, entomopathogenic fungi

DAYA MANGSA PREDATOR Harmonia axyridis TERHADAP KUTU DAUN Mizus persicae PADA TANAMAN JERUK Otto Endarto; Susi Wuryantini
JURNAL AGRONIDA Vol. 5 No. 2 (2019)
Publisher : Universitas Djuanda Bogor

The research aims to determine the prey abilily and preferences of Harmonia axyridisagainst Mizus persicae on citrus. The study was conducted at the screen house and EntomologyLaboratory of the Research Institute for Citrus and Sub-Tropical Fruits, in January-March 2016.The test for prey ability used the first larval stage of H. axyridis to imago for 30 individuals in eacharena. Data were analyzed by counting of M. persicae which eaten per day from each stage, i.e.larva instar, and H. axyridis imago, as well as the average prey during their life cycle. The resultsshowed that the highest prey of H. axyridis occurred in instar larvae 4, which reached 428.3% ofis body weight, with a number of preys 9.81 ± 1.01 head / day. Preferences of H. axyridis in instarslarvae 1,2, and 3 were instar nymphs 1 from M. persicae, and for H. axyridis the instar larvae 4and imago preferred of instar nymph 4. H. axyridis had a life cycle with a range of 6.6 -9.7 days.The duration of egg stage of the H. axyridis was 3 days, the larvae consisted of 4 instars, theduration of the stage for each instar was 2.4 days for instar 1 and 2; 5 days for instar 3; 12 daysfor instar 4, and 18.5 days for imago.Keywords: Prey, predator Harmonia axyridis, Mizus persicae, citrus

TOKSISITAS BIOINSEKTISIDA EKSTRAK KULIT JERUK TERHADAP KUTU LONCAT JERUK Diaphorina citri Kuwayama (Hemiptera: Psyllidae) SEBAGAI VEKTOR PENYAKIT CVPD Susi Wuryantini; Harwanto Harwanto; Rizky Arya Yudistira
JURNAL AGRONIDA Vol. 5 No. 2 (2019)
Publisher : Universitas Djuanda Bogor

Diaphorina citri is an important pest in citrus plants because of its role as a vector of CitrusVein Phloem Degeneration (CVPD) disease. The study was a follow-up test of the preliminarytests carried out previously. The aim of this study was to determine the effective concentrationof bioinsecticide of Japansche Citroen (JC) orange peel extract in controlling D. citri in thephases of nymph and imago. The concentration tested was 0% (control); 0.01%; 0.1%; 1%;and 10% (v / v) with a comparison of imidacloprid insecticide 0.1%. The test results showedthat the JC citrus peel extract at a concentration of 0.1 effectively controlled the D. citri nymphstarting at 96 hours after application (HAA) with a mortality of 69%. The concentration of 1%was effective from 24 HAA with a mortality of 65% and thereafter reaching up to 100% at 120HAA, this was not significantly different from the 10% concentration treatment andimidacloprid insecticide 0.1%, yet was significantly different from other treatments andcontrols. Citrus peel extract was effective for controlling imago of D. citri on 10% concentration. The concentration that was able to provide mortality above 50% was the highestconcentration of 10% at 96, 120 and 144 HAA. The results of the probit analysis showed thatthe LC50 values at 24 HAA on nymphs was 1.86 and the LC90 value was 9.10. The treatment onimago the LC50 at 48 HAA was 21.34 and the LC90 was not achieved. The results of thecalculation of the LC50 and LC90 values showed that the value in nymphs was smaller than inimago. This showed that the bioinsecticide of JC citrus peel extract was more effective againstnymphs than imago of D. citri. Bioinsecticide of JC citrus peel extract was effective againstpsyllid nymph of D. citri at concentrations of 0.1 and 1% and against the imago phase waseffective at 10%.Keywords: Toxicity, citrus peel extract, Diaphorina citri

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