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Morfofisiologi dan Profil Biokimia Darah Kalong Kapauk (Pteropus vampyrus) dari Wilayah Pesisir Kabupaten Garut Danang Dwi Cahyadi; . Nurhidayat; Chairun Nisa'; . Supratikno; Savitri Novelina; Heru Setijanto
Acta VETERINARIA Indonesiana Vol. 6 No. 1 (2018): Januari 2018
Publisher : IPB University

Aktivitas terbang pada kelelawar membutuhkan energi paling banyak dibandingkan dengan aktivitas lokomosi lainnya. Morfofisiologi dan profil biokimia darah diduga memiliki peranan penting terhadap kemampuan terbang hewan tersebut. Penelitian ini bertujuan untuk mengetahui karakteristik morfofisiologi eritrosit dan profil biokimia darah P. vampyrus. Penelitian ini menggunakan 15 ekor kelelawar dewasa dengan bobot badan antara 669,7 g sampai 1211,5 g (x̄ = 957,51 ± 177,52 g). Sampel darah diambil secara langsung melalui ventrikel kiri jantung. Pengamatan dan pengukuran terhadap preparat ulas darah menunjukkan bahwa morfologi eritrosit P. vampyrus mirip dengan mamalia secara umum dan mempunyai diameter rata-rata 7,15 ± 0,45 µm. Koefisien variasi ukuran eritrosit (RDWc) hewan ini sebesar 18,11 ± 1,16%. Pemeriksaan hematologi yang dilakukan menggunakan automated counter menunjukkan bahwa total eritrosit (8,89 ± 1,36 106/µl), konsentrasi hemoglobin (14,33 ± 2,38 g/dl), dan nilai hematokrit (42,13 ± 6,49%) P. vampyrus relatif lebih tinggi dibandingkan dengan mamalia pada umumnya. Neutrofil dan limfosit merupakan komponen yang mendominasi jumlah leukosit. Adapun persentase jumlah neutrofil lebih tinggi dibandingkan dengan jumlah limfosit. Penelitian ini memberikan informasi dasar yang dapat mendukung penelitian terkait dengan kemampuan terbang dari P. vampyrus.

PF-7 Pre Slaughter Stunning of Ruminant Slaughter Based on MUI HAS 23103 2012, MS 1500 2009 and SNI 99003 2018 . Supratikno; Danang Dwi Cahyadi; Heru Setijanto
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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The debate about the use of pre slaughter stunning (PSS) in halal slaughter is still an interesting topic to discuss due to the difficulties to determine the stunned animal are unconscious or dead. Indonesia and Malaysia as a country with majority Muslim population have recognized non-penetrative PSS (NPPSS) as a method in halal slaughter. Indonesian Council of Ulama (MUI) issued the Halal Assurance System (HAS 23103 2012)[1] as guidelines for animal slaughter process, while Department of Standard Malaysia issued Malaysian Standard (MS) 1500 2009[2], which regulate Halal Food-Production, preparation Handling and Storage-General Guidelines. Recently, the Indonesian National Standardization Body issued Indonesia's National Standard for ruminant halal slaughter (SNI 99003 2018) [3]. This article will discuss mechanical NPPSS based on these three guidelines in animal welfare and halal perspective

PF-30 Anatomical Characteristic of Hindlimb Skeleton of Sumatran Rhino (Dicerorhinus sumatrensis) . Nurhidayat; Eni Puji Lestari; Danang Dwi Cahyadi; Chairun Nisa'; . Supratikno; Savitri Novelina; Heru Setijanto
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Sumatran rhino (Dicerorhinus sumatrensis) is an endangered animal in Indonesia. Currently, Sumatran rhino only found on Sumatera and Kalimantan in very small populations. These herbivorous animals are classified into the order of Perissodactyla (odd-toed animals) and family Rhinocerotidae [1]. Sumatran rhino is one of the largest living land mammals, reaching 1000 kg [1], although this species has the smallest body weight among all extant rhinos. This animal has a round and long body shape, relatively short legs with three digits on each leg. These body structures correspond to their habitat in the highlands, so the Sumatran rhino has an excellent ability to pass steep terrain [2]. For this reason, a strong hind limb structure is needed to push the body when walking, running and climbing the steep slopes. Therefore, this study was carried out in order to analyze the correlation between the Sumatran rhino’s hind limb skeleton and its functional roles.

AH-02 Cutaneous Muscle of Javan Porcupines (Hystrix javanica) Supratikno Supratikno; Halim Bakti Harjo; Danang Dwi Cahyadi; Srihadi Agungpriyono
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Cutaneous Muscle of Javan Porcupines (Hystrix javanica)

AH-03 Anatomical Characteristic of Forelimb Skeleton of Sumatran Rhino (Dicerorhinus sumatrensis) Nurhidayat Nurhidayat; Eni Puji Lestari; Chairun Nisa'; Danang Dwi Cahyadi; Supratikno Supratikno
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Anatomical Characteristic of Forelimb Skeleton of Sumatran Rhino (Dicerorhinus sumatrensis)

AH-04 Anatomical Characteristic of the Pelvic and Thigh Region Muscles of Javan Pangolin, Manis javanica Chairun Nisa'; Singgih Pratiknyo Sundawa; Supratikno Supratikno; Danang Dwi Cahyadi
Media Veteriner Proceedings of The 5th Congress of Asian Association of Veterinary Anatomists (Asian AVA) 2015
Publisher : Media Veteriner

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Anatomical Characteristic of the Pelvic and Thigh Region Muscles of Javan Pangolin, Manis javanica

Studi Mikroanatomi Pankreas Kodok Lembu Menggunakan Metode Pewarnaan Baku dan Immunohistokimia (MICROSCOPICAL STUDY OF PANCREAS OF BULLFROG USING CONVENTIONAL AND IMMUNOSTAINING METHODS) I Ketut Mudite Adnyane; Supratikno .; Adi Winarto; Srihadi Agungpriyono
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Morphology, distribution and relative frequency of endocrine cells in the pancreas Bullfrog (Ranacatesbeiana) were studied using conventional and immunohistochemical methods. Samples pancreas takenfrom ten adult Bullfrogs (five males and five females). In general, pancreas of the Bullfrog consists ofexocrine portion, endocrine portion (Langerhans islets) and ducts. The Langerhans islets were distributedamong the exocrine portion of pancreas. Endocrine cells in the pancreas of Bullfrog were polimorph, round,oval or triangular in shapes with bipolar cytoplasmic granules. Glucagon cells were distributed mainly inthe peripheral, insulin cells in the center while the somatostatin cells in the area between glucagon andinsulin cells of Langerhans islet. The number of the glucagon cells were higher compare to the number ofinsulin and somatostatin cells.

CHARACTERISTIC OF SKIN MORPHOLOGY OF SUNDA PORCUPINE (Hystrix javanica) WITH SPECIAL REFERENCE TO THE CONNECTIVE TISSUE Andhika Yudha Prawira; Desrayni Hanadhita; Anisa Rahma; Supratikno Supratikno; Savitri Novelina; Srihadi Agung Priyono
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

This study was conducted to investigate the histological characteristic, type, and distribution of connective tissue in Sunda porcupine skin. The investigation was carried out in three adult of sunda porcupines at microscopic level using hematoxylin eosin, Masson thrichrome, Verhoeffs van Gieson, alcian blue pH 2.5 and periodic acid Schiff staining methods. Skin consists of epidermis, dermis hypodermis, and subcutaneous muscle. Quill follicles were the main and dominant structure as well as the specific characteristic on Sunda porcupine skin. The connective tissue was distributed well in basal membrane, dermis, quill follicle, and hypodermis with various intensity and density. The collagen was the main fiber found in the skin while the elastin fiber was not observed. The acid carbohydrate was found distributed well in the skin while the neutral carbohydrate was not detected in this study. In addition the fibers of connective tissue associated with the adipose tissue which found plentifully in quill follicles and hypodermis. The present results showed that the wide distribution of connective tissue might have an important role on the wound healing physiology of Sunda porcupine skin.

Development of Adrenocorticotrophic Hormone Cells of Adenohypophysis–Adrenal Cortex Axis of Long-tailed Monkey (Macaca fascicularis) during Pre and Postnatal Period Nurhidayat Nurhidayat; Ika K. Syarifah; Supratikno Supratikno; Sri Wahyuni
Jurnal Kedokteran Hewan Vol 2, No 2 (2008): September
Publisher : Universitas Syiah Kuala

The objective of this study is to observe the relationship of development of adrenocorticotropic hormone (ACTH) cells of adenohypophysis–adrenal cortex axis during pre and postnatal period of longtailed monkey (Macaca fascicularis). Five fetuses aged 70, 85, 100, 120, and 150 days of gestation and 2new born aged 10 and 105 days old were used as research materials. The sections of the pituitary gland stained immunohistochemically using human ACTH antiserum and the adrenal glands stained by Hematoxylin-Eosin procedures. At the fetus 120 days old, the ACTH cells and blood vessel of fetaladenohypophysis developed well and its similar that found in older age. This condition suggested distributing the ACTH to whole body optimally. At the same time, the adrenal cortex cells of fetus 120 days old were active in proliferation and differentiation processes to form transition zone that lies between definitive and fetal zones, and central vein also developed in adrenal gland. In fetus aged 150 days, the cortex adrenal structure was similar with found in older fetus. The adenohypophysis ACTH cells developed earlier than adrenal cortex and their secretion suggested influencing the development of adrenal cortex cells of long-tailed monkey.

CHARACTERISTIC OF SKIN MORPHOLOGY OF SUNDA PORCUPINE (Hystrix javanica) WITH SPECIAL REFERENCE TO THE CONNECTIVE TISSUE Andhika Yudha Prawira; Desrayni Hanadhita; Anisa Rahma; Supratikno Supratikno; Savitri Novelina; Srihadi Agung Priyono
Jurnal Kedokteran Hewan Vol 12, No 1 (2018): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i1.6892

This study was conducted to investigate the histological characteristic, type, and distribution of connective tissue in Sunda porcupine skin. The investigation was carried out in three adult of sunda porcupines at microscopic level using hematoxylin eosin, Masson thrichrome, Verhoeffs van Gieson, alcian blue pH 2.5 and periodic acid Schiff staining methods. Skin consists of epidermis, dermis hypodermis, and subcutaneous muscle. Quill follicles were the main and dominant structure as well as the specific characteristic on Sunda porcupine skin. The connective tissue was distributed well in basal membrane, dermis, quill follicle, and hypodermis with various intensity and density. The collagen was the main fiber found in the skin while the elastin fiber was not observed. The acid carbohydrate was found distributed well in the skin while the neutral carbohydrate was not detected in this study. In addition the fibers of connective tissue associated with the adipose tissue which found plentifully in quill follicles and hypodermis. The present results showed that the wide distribution of connective tissue might have an important role on the wound healing physiology of Sunda porcupine skin.

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