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Biosupplementation of Ethanolic Extract of Cashew Leaf (Anacardium occidentale L.) to Improve Weight Gain and Immunity of Jawa Super Chicken Manesta Edelweis Jingga; Haris Setiawan; Ardaning Nuriliani; Hendry Trisakti Saragih
Acta VETERINARIA Indonesiana Vol. 7 No. 2 (2019): Juli 2019
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1167.73 KB) | DOI: 10.29244/avi.7.2.57-65

Abstract

Effect of ethanolic extract of cashew leaf (Anacardium occidentale L.) in the feed on the weight gain and immunity were investigated on Jawa Super Chicken. Ninety chicken were divided into 6 groups those were basal feed group and 5 different concentration of chasew leaf extract groups. Experiment was conducted for 16 days started by 2 days acclimatization after hatching. Body weight was measured every 3 days starting from 0th day. On 16th day, the chicken were sacrificed, spleen and bursa of Fabricius were collected to quantify some histological parameters such as white pulp and follicle area as well as follicle thickness of those organs. The data were analyzed by regression and one way ANOVA followed by Tuckey test. Histological analysis was performed using optilab and Image Raster software. The results showed that growth and development of immune system of chicken increased significantly as could be seen in body weight, organs weight and index, as well as histological parameters. The regression results showed that spleen and bursa of Fabricius weight influenced increasing of body weight by 64.2% and 38.4% respectively. It could be concluded that biosupplementation of cashew leaf extract in basal feed could increase growth and immunity of Jawa Super Chicken.
The Effect of Coffee Fruit Skin Extract on Sperm Characteristics And Testicular of Mice With Ethanol-Induced Haris Setiawan; Rita Maliza; Syaiful Adam Maulana; Muhammad Ilham Hisbullah
Jurnal Biodjati Vol 5, No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.9280

Abstract

Coffee fruit skin contains antioxidant compounds that can repair damaged tissues, especially those of reproductive or-gans. This study was designed to assess to what extent the metha-nolic extract of coffee fruit skin affected sperm characteristics and testicular histology in mice receiving 15% ethanol for 15 days. It used 25 male mice aged four months and employed a completely randomized design with two controls (K) and three treatments (P), namely K1 (without ethanol), K2 (15% ethanol), P1 (15% ethanol and the methanolic extract of coffee fruit skin, or MECS, at 125 mg/ kg BW), P2 (15% ethanol and MECS 250 mg/kg BW), and P3 (15% ethanol and MECS 500 mg/kg BW). These treatments were administered orally with a 1 mL disposable syringe for 15 days. The parameters observed were sperm characteristics (viability, morphology, and sperm count) and the microscopic structure of the testicles (lu-men area, diameter, and area of seminiferous tubules). These data were then analyzed using the one-way ANOVA, continued with the LSD and Duncan’s tests (P <0.05). The results showed that P2 (250 mg/kg BW) substantially improved sperm count (86.5x105±1.73 cells/ mL), sperm viability (87.26±0.05%), and the proportion of normal sperm morphology (93.33±0.004%) of mice with 15% ethanol-in-duced sperm damages. Testicular histology also confirmed improve-ments in spermatogenic cells, as evident from the lumen, diameter, and area seminiferous tubules after receiving P1 and P2. In conclu-sion, the optimum dose of the coffee skin extract for improving sperm quality and microscopic structures of mice testicles is 250 mg/kg BW. 
The Effect of Coffee Arabica (Coffea arabica L.) Fruit Skin Extracts on Small Intestine Morphometry of mice (Mus musculus L.) with Ethanol-Induced Rita Maliza; Febriofca Galih Yatalaththov; Haris Setiawan; Listiatie Budi Utami
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151111571-0-00

Abstract

Consumption of alcoholic beverages can increase ROS and inflammation. In the duodenal, the consumption of alcohol can result in erosions, bleeding, and mucosal injury. Arabica coffee fruit skin contains antioxidant substances, which are expected to have a good role as an antioxidant effect. Currently, unknown effects of alcohol and extract of Arabica coffee fruit skin administration on the damage of the small intestine of mice (Mus musculus L.). This study used an experimental design, and 25 males of mice were divided into five groups of treatment for 15 days. After the treatment, the mice at necropsied and the small intestine were taken for histopathological examination using hematoxyline-eosin staining. The Data analyzed used One Way ANOVA and Duncan's tests. Histopathological observations showed a dose of 250 mg/kg BW experienced the most significant improvement damage of the small intestine of mice compared to other treatments. Histomorphometry measurement, The dose of 250 mg/kg BB showed the high of villi 272,10 ± 13,36 µm, (P<0.05), duodenal villi crypt depth 117,33 ± 30,35 µm (P<0.05) and surface area of small intestinal villi 7,66 ± 0,55 mm2 (P<0.05) was similar with negative control. The conclusion of this study is a dose of 250 mg/kg BW treatment was able to improve the histopathology and morphometry small intestine of mice that were damaged due to consumption of ethanol 15%.
KEANEKARAGAMAN JENIS SEMUT (HYMENOPTERA: FORMICIDAE) DI SEKITAR KAMPUS 4 UNIVERSITAS AHMAD DAHLAN YOGYAKARTA Ichsan Luqmana Indra Putra; Haris Setiawan; Novilia Suprihatini
Biospecies Vol. 14 No. 2 (2021): Biospecies, July 2021
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v14i2.12905

Abstract

Keanekaragaman adalah jenis-jenis makhluk hidup yang ada di bumi, baik di daratan, lautan, maupun tempat lainnya, salah satunya semut. Semut dapat ditemukan di berbagai habitat, baik di dataran rendah ataupun dataran tinggi. Penelitian ini bertujuan untuk mengetahui tingkat keanekaragaman semut yang berada disekitar kampus 4 UAD Yogyakarta dan mengetahui spesies semut dominan yang ditemukan. Area pengambilan sampel mewakili 4 area sekitar kampus yaitu persawahan, perumahan, kampus, dan lahan kosong. Setiap area pengambilan sampel ditentukan 2 plot dengan ukuran 700 m2 (35 m x 20 m) yang tersebar pada area pengambilan sampel. Kemudian di dalam plot ditentukan 5 subplot dengan ukuran 150 m2 (15 m x 10 m) yang tersebar di dalam plot. Metode yang digunakan pada penelitian ini adalah pengambilan sampel dengan metode tidak langsung dengan menggunakan jebakan sugar trap dan bait trap. Lima trap dipasang di setiap subplot, sehingga total terdapat 25 jebakan pada masing-masing plot. Semut yang didapatkan kemudian diidentifikasi dengan melihat ciri morfologinya sampai tingkat spesies. Hasil identifikasi kemudian ditabulasikan untuk kemudian dihitung tingkat keanekaragaman semut. Perhitungan tingkat keanekaragaman tersebut dihitung dengan menggunakan rumus Shannon-Wiener dan untuk menghitung dominansi semut menggunakan formula Ludwig dan Reynolds (1981). Hasil penelitian ini, didapatkan tingkat keanekaragaman semut disekitar kampus 4 UAD memiliki tingkat rendah dengan nilai H’ 0,78 yang disebabkan oleh pengaruh dari alih fungsi lahan dan diperoleh spesies semut yang paling melimpah adalah Monomorium minimum dengan jumlah 3734 individu dan yang tidak melimpah yaitu spesies Cardiocondyla elegans dengan jumlah 1 individu.
Potensi Imunomodulator Herbal Ekstrak Etanol Daun Pepaya Varietas Calina terhadap Struktur Jaringan Limpa Tikus Putih Galur Wistar Haris Setiawan; Sri Wijayanti Wulandari; Aritasya Nur Fitriyani
Jurnal Veteriner Vol 22 No 4 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.494 KB) | DOI: 10.19087/jveteriner.2021.22.4.531

Abstract

Tanaman pepaya varietas calina (Carica papaya L. Var. calina) merupakan salah satu jenis tanaman pepaya yang banyak dibudidayakan di Indonesia. Tanaman ini memiliki kandungan senyawa aktif yang dapat berperan dalam meningkatkan kerja imun seperti flavonoid, polifenol, tanin dan saponin. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak etanol daun pepaya varietas calina sebagai imunomodulator alami terhadap struktur jaringan limpa tikus putih galur wistar (Rattus norvegicus Berkenhout, 1769). Penelitian menggunakan 24 ekor tikus wistar yang dibagi ke dalam empat perlakuan pemberian ekstrak yaitu kontrol (dosis 0 mg/kg BB), P1 (dosis 100 mg/kg BB), P2 (dosis 200 mg/kg BB), dan dosis P3 (300 mg/kg BB) selama 30 hari per oral. Parameter yang diamati meliputi bobot badan, rasio bobot limpa dan histologi limpa yang terdiri dari luas dan diameter pulpa putih serta luas germinal center. Tikus dikorbankan nyawanya pada hari ke-31, kemudian organ limpa diambil dan difiksasi dengan neutral buffer formaline 10%. Limpa dibuat sediaan histologis dengan menggunakan blok parafin dan pewarnaan hematoxylin-eosin. Data dianalisis dengan menggunakan sidik ragam satu arah kemudian dilanjutkan uji jarak berganda Duncan untuk mengetahui tingkat signifikansi antar perlakuan. Hasil penelitian menunjukkan bahwa seluruh perlakuan memberikan pengaruh yang signifikan (P<0,05), dalam hal menambah diameter dan memperluas pulpa putih serta memperluas germinal center pada limpa dibandingkan dengan kontrol. Bobot badan dan rasio organ limpa tidak menunjukan perbedaan secara siginifikan antar perlakuan (P>0,05). Simpulan penelitian adalah ekstrak etanol daun pepaya varietas calina memiliki potensi sebagai agen imunomodulator alami.
Serbuk Daun Jambu Biji Memperbaiki Performans Pertumbuhan dan Morfologi Duodenum Ayam Jawa Super (GUAVA LEAF POWDER IMPROVING PERFORMANCE OF GROWTH AND DUODENUM MORPHOLOGY OF JAWA SUPER CHICKEN) Haris Setiawan; Listiatie Budi Utami; Muhammad Zulfikar
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.235 KB) | DOI: 10.19087/jveteriner.2018.19.4.554

Abstract

Natural bioactive compounds used as feed supplements in feed could increase chicken productivity in the livestock industry. The research aims to study the use of guava leaf powder as a source of bioactive compounds in the feed on growth performance and duodenum morphology of Jawa Super Chicken. This study used 80 days old chick (DOC) Jawa Super chickens reared until the age of 16 days. The study used a completely randomized design, with the addition of treatments guava leaf powder in the basal feed from 0 g/kg diet (control); 2.5 g/kg of feed (P1); 5 g/kg of feed (P2); 10 g/kg of feed (P3). Parameters observed included growth performance, body components, digestive organ components and duodenum morphology consisting of crypt depth, height, and area of villi. Data was analyzed by using one-way analysis of variance then continued with LSD and Duncan test (Pd”0.05). The results showed that guava leaf powder could significantly increase the height of villi, and components of digestive organs in chickens. The results of the duodenum morphology observation showed that the P2 and P3 are better than the control (Pd”0.05). The growth performance and body components of the Jawa Super chicken did not show a significant difference (Pd”0.05) between the control and the treatment. In conclusion, the guava leaf powder with a dose of 10 g/kg body weight can increase duodenal villi growth, the weight of intestine and liver without influence the weight of Jawa Super chickens aged 16 days.
The Effectiveness Combination of Maggot with Commercial Feed on Growth, Structure of Intestine and Skeletal Muscle Mutiara Catfish Haris Setiawan; Ichsan Luqmana Indra Putra; Ridwan Alfatah; Akhmad Nizzar Nasikhudin
Journal of Aquaculture and Fish Health Vol. 11 No. 1 (2022): JAFH Vol. 11 No. 1 February 2022
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v11i1.26063

Abstract

Maggot (Black soldier fly larvae) as an alternative source of protein on feed can increase fish productivity. The research aims to study maggot flour as an alternative source of protein in feed on the growth, structure of the intestine organs and skeletal muscles of Mutiara catfish (Clarias gariepinus Burchell, 1822). The research used 144 Mutiara catfish that were kept for 21 days. The research used a completely randomized design consisting of 4 groups, namely 0% maggot in feed (Control), 25% maggot in feed (P1), 50% maggot in feed (P2), 75% maggot in feed (P3). The parameters consist of the growth, the structure of intestine and skeletal muscle of Mutiara catfish. Growth observations consist of measurements of the absolute length and weight of the fish. Intestine observations consisted of length and weight, as well as tissue structure consisting of villi height, crypt depth, villi / crypt ratio and villi area area. Skeletal muscle observations consist of muscle weight and tissue structure consisting of the diameter and area of muscle fibers. Data analysis was performed with one-way anova, followed by the Duncan Test. The results showed that 75% maggot feed gave significant results on absolute weight, structure of intestine and muscle organs compared to other treatments (P <0.05). The absolute length did not provide a significant difference between treatments (P> 0.05). The conclusion shows that 75% maggot feed can be used as an alternative source of feed protein because it can increase the growth, structure of the intestine organs and skeletal muscles of the Mutiara catfish.
EFEK ANTISPERMATOGENIK EKSTRAK ETANOL DAUN PEPAYA CALINA TERHADAP KUALITAS SPERMA DAN MORFOLOGI EPIDIDIMIS TIKUS WISTAR haris setiawan
BERITA BIOLOGI Vol 21, No 1 (2022)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i1.4175

Abstract

Indonesia merupakan negara dengan angka kelahiran yang relatif tinggi, sehingga dibutuhkan alternatif dalam menurunkan jumlah penduduk seperti penggunaan alat kontrasepsi berbahan herbal. Penelitian bertujuan mengetahui pengaruh ekstrak etanol daun pepaya Calina terhadap kualitas sperma dan morfologi epididimis tikus wistar. Penelitian menggunakan 24 tikus wistar, dibagi menjadi 4 kelompok terdiri dari Kontrol (akuades), ekstrak dosis 100 mg/Kg BB, 200 mg/Kg BB, 300 mg/Kg BB yang diberikan menggunakan sonde lambung (1ml) selama 30 hari. Pada hari ke-31 tikus dibedah dan diambil cauda epididymis untuk dilakukan pengamatan kualitas sperma dan pembuatan preparat histologis dengan metode parafin (pewarnaan HE). Parameter kualitas sperma terdiri dari motilitas, viabilitas, jumlah, dan morfologi sperma. Parameter morfologi epididimis terdiri dari tebal sel epitel, diameter dan luas epididimis. Seluruh parameter dianalisis menggunakan uji one way Anova dan dilanjutkan uji Duncan dengan selang kepercayaan 95%. Hasil menunjukan pemberian ekstrak dosis 100-300 mg/Kg BB berbeda secara signifikan dibandingkan kontrol dengan menurunkan parameter motilitas, jumlah, viabilitas dan morfologi sperma (P<0,05). Morfologi epididimis menunjukan penurunan tebal epitel, diameter dan luas epididymis pada dosis 100-300 mg/kgBB dibandingkan dengan kontrol (P<0,05). Kesimpulan menunjukan ekstrak etanol daun pepaya Calina dosis 100 mg/kgBB memiliki potensi sebagai agen antispermatogenik ditandai dengan penurunan kualitas sperma dan morfologi epididymis tikus wistar.
The Protective Effect of Tropical Fruit Juice on Histopathological of Kidney Wistar Rats After Exposed Cigarette Smoke Novi Febrianti; Haris Setiawan; Rahmat Krestianto
Ahmad Dahlan Medical Journal Vol. 1 No. 2 (2020): November 2020
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3001.949 KB) | DOI: 10.12928/admj.v1i2.3132

Abstract

Cigarette smoke is a toxic compound that can interfere with physiological effects and damage the organ systems in the body. The effects of free radicals arising from cigarette smoke can be neutralized by consuming antioxidant compounds. The research aims to look at the protective effects of mixed juice from tropical fruits that grow in Indonesia to the histopathological picture of the kidney of wistar rats exposed to cigarette smoke. There were 35 rats used and divided into 5 treatment groups consisting of K (without treatment), K- (cigarette smoke exposed), P1 (strawberry and guava juice mixed juice 3ml), P2 (strawberry and papaya mixed juice 3ml), P3 (P3 (strawberry and apple mixed juice 3ml). In P1, P2 and P3 before being given a mixture of fruit juice, rats were exposed to cigarette smoke first. The treatment was carried out for 40 days by oral gavage, then on the 41st day the rats were dissected and their kidney organs were taken for observation and histopathological preparations. Histopathology preparations were made by the paraffin method and Haematoxylin Eosin staining. The cell damage data is then analyzed by comparing the kidney cells from the control group with the treatment group and scoring damage followed by the percentage of kidney damage. Data were statistically tested using ANOVA followed by Post Hoc LSD. The results showed that mixed tropical fruit juice could protect kidney cell damage by decreasing the percentage of hydropic degeneration, necrosis and infiltration of inflammatory cells compared with negative controls (P <0.05). There is no significant difference between P1, P2 and P3 in reducing cell damage. The conclusion of the study is that all combinations of tropical fruit juice can protect cell damage caused by free radicals from exposure to cigarette smoke in wistar rats.
The Effect of Arabica Coffee Fruit Skin Extract (Coffea arabica L.) on the Histopathology of Mice (Mus musculus L.) Liver Induced by Ethanol Dini Cahyani; Rita Maliza; Haris Setiawan
Journal of Biotechnology and Natural Science Vol. 1 No. 1 (2021)
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.244 KB) | DOI: 10.12928/jbns.v1i1.4734

Abstract

Excessive alcohol consumption can lead to serious live injury. Alcohol is a source of free radicals that can cause oxidative stress. The provision of antioxidants can counter the effects of free radicals that enter the body. The skin of the Arabica coffee fruit contains antioxidant compounds that can be used as a hepatoprotective in the liver. The skin of the Arabica coffe fruit contains antioxidant compounds that may have a hepatoprotective effect on the liver. This study aimed to determine the effect of extract of arabica coffee fruit skin on necrosis, infiltration and haemorrhage in the liver tissue of 15% EtOH-induced mice for 15 days. The animals used for this study were eight weeks old male Balb/c strain mouse weighing approximately 30-40 grams. This research is an experimental study. There were five groups: the control group (K), the 15% ethanol treatment group (E), and the ethanol-induced group and given different doses of arabica coffee skin extract: 125 mg/kg BW. (A), 250 mg / kg BW (B) and 500 mg / kg BW (C). Observation and identification of necrosis, hepatocyte infiltration and haemorrhage were carried out by using Hematoxylin-eosin staining. The data analysis used was One way ANOVA and continued by Duncan Multiple Range Test (DMRT). The histopathological observations showed that the extract of arabica coffee fruit skin at a 250 mg/kg BW dose reduced the number of cell necrosis. There was no infiltration of inflammatory and hemorrhagic cells in the liver of 15% EtOH-induced mice. This study concludes that the extract of the skin of the Arabica coffee fruit has hepatoprotective potential in 15% EtOH-induced mice.