Reza Yesica
Fakultas Kedokteran Hewan, Universitas Brawijaya, Malang

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Prevalence, Identification and Geographical Distribution of Eimeria spp. in Wild Rodents in Malang, East Java Reza Yesica; Shelly Kusumarini; Ida Wisesa
Acta VETERINARIA Indonesiana 2021: Special Issues
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi...78-85

Abstract

Eimeria is a genus of apicomplexan parasites that infected various species and known as the enteric monoxenous coccidian. This study aims to determine the prevalence, identification and geographical distribution study of gastrointestinal protozoa Eimeria spp. in wild rodents in Malang city, East Java. A total of 74 rodents were collected from different part of Malang, using single live traps. Rats were euthanized with ketamine and xylazine. Samples of stools were taken from gastrointestinal tract. Coprological procedure using floatation method lead to the demonstration of Eimeria spp. oocysts in the faecal samples examined at 400 and 1000 magnifications of light microscope. Cultivation in dichromate potassium 2.5% was carried out for all of coccidian positive samples. The identification is based on morphology and morphometry. Eimeria identification data was analyzed descriptively, while the analysis of species and gender of rodents on prevalence used chi square. Result, the prevalence of Eimeria spp. in Malang is 11 (14.9%). It was suggesting coccidiosis caused by Eimeria nieschulzi. In addition, Chi square analysis showed that species of rats did not have a significant relationship (P>0.05) to the incidence of Eimeria infection, however it has significant correlation between gender of rats and Eimeria infection (P<0.05). The geographical of Eimeria spp. spots were showed in 8 Sampling area, in Lowokwaru, Klojen, Kedung kandang and Sukun sub district. Findings of this study showed that wild rodents in Malang city are infected with intestinal coccidian parasites.
Preliminary Study: Detection of Ecto and Endoparasites Among Wild Rats From Urban Area in Blimbing, Malang, East Java shelly kusuma; Reza Yesica; Ida Bagus Gde Rama Wisesa; Jenny Hermanto; Yustia Nurholizah; Maria Widyaneni Trinastuti
Acta VETERINARIA Indonesiana 2021: Special Issues
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi...95-101

Abstract

Zoonotic parasitic infections is one of the global public health issues. The complex parasite transmission allows for the relationship between people, animals, vectors, and the environment. The existence of rat in the environment has an important role as a host and reservoir for various types of ecto and endoparasites. This study was conducted to collect informative data on the parasitic infection of wild rats in Blimbing sub-district, Malang city, East Java. A total number of eight wild rat were captured using live-traps from landfills during 4 days. They were classified by sex, weight and body length. The rats were anesthetized, collecting for any ectoparasites and then their carcasses were dissected for examinations of endoparasites. The result of this study show succesfull live-trap of rodents including Rattus norvegicus (87.5%) and Suncus murinus (12.5%). 50% of sampled rodents were male, 37.5% were female, and 12.5% of female the musk shrew. The presence of helminthes infection in all wild rats, namely Hymenolopis nana, Syphacia obvelata, Nippostrongylus brasiliensis. Furthermore, the ectoparasites identification were Xenopsylla cheopis, Echinolaelap echidninus, and blood protozoa identifications Trypanosoma lewisi. Based on the results show 100% of wild rats positive infections of endo-ectoparasites. This study indicates to continuous study among rodents’ parasites in wild rats in different urban areas and analysis of their potential impact on public health.
Data Prevalensi, Pemetaan Spasial, Analisis Morfologi, dan Morfometrik Trypanosoma lewisi Pada Tikus Liar Di Malang Reza Yesica; Yustia Nur Holizah; Herlina Pratiwi; Andreas Bandang Hardian; Shelly Kusumarini R; Ida Bagus Gde Rama Wisesa
Acta VETERINARIA Indonesiana Vol. 10 No. 1 (2022): Maret 2022
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.10.1.71-79

Abstract

Trypanosomiasis merupakan penyakit yang disebabkan oleh protozoa darah ekstraseluler berflagela yaitu Trypanosoma sp. Trypanosoma lewisi adalah parasit darah pada tikus, yang ditularkan oleh pinjal Xenopsylla cheopis. Walaupun parasit ini bersifat non patogen, tetapi keberadaannya dapat mengancam kesehatan manusia. Kasus infeksi T. lewisi pada manusia telah dilaporkan di Thailand dan India, yang mengindikasikan bahwa penyakit ini dapat menginfeksi manusia dalam beberapa keadaan yang belum diketahui. Penelitian ini bertujuan untuk mengidentifikasi T. lewisi berdasarkan morfologi dan morfometrik serta dilakukan perhitungan prevalensi dan pemetaan spasial kasus infeksi T. lewisi pada tikus liar di Malang. Sebanyak 74 ekor tikus dikumpulkan dari berbagai wilayah di Malang dengan menggunakan perangkap hidup tunggal. Hasil penelitian menunjukkan prevalensi infeksi T. lewisi pada tikus liar di Malang dari bulan Agustus sampai Oktober 2020 sebesar 17,5%. Analisis data menggunakan uji chi-square dan uji Fisher, diperoleh hasil tidak ada hubungan atau korelasi yang signifikan antara infeksi T. lewisi dengan jenis kelamin dan umur tikus. Morfologi T. lewisi memiliki posterior tipis dengan kinetoplas oval di sub-terminal, dan nukleus di anterior. Secara morfometrik, T. lewisi memiliki panjang rata-rata 33,19 μm, lebar 3,52 μm, panjang inti 7,82 μm, lebar inti 3,05 μm, panjang kinetoplas 5,25 μm, serta jarak inti ke kinetoplas 10,79 μm. Dari hasil di atas dapat disimpulkan bahwa Trypanosoma lewisi menginfeksi tikus liar dan hasil gambaran geografis didapatkan bahwa terdapat risiko penyebaran penyakit trypanosomiasis di wilayah Malang.
Evaluasi Pemberian Obat Diminazene Aceturate Secara In Vivo Pada Mencit (Mus musculus) yang Diinfeksi Isolat Trypanosoma evansi Reza Yesica; Bambang Sutrisno; Wisnu Nurcahyo
Jurnal Sain Veteriner Vol 39, No 2 (2021): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.43100

Abstract

Abstract Surra's disease is caused by Trypanosoma evansi parasite has been established as one of the strategic infectious animal diseases. Drug resistance in this case is one of the major challenges in handle and control them. The aim of this study is to evaluate the provision drug resistance diminazene aceturate (Tryponil®) on Trypanosoma evansi isolate from Pemalang and Brebes Central Java province with in vivo test in mice. Total 50 mice, BALB / c strain, male, 2 months, body weight ± 30 gram are obtained from LPPT-UGM, adapted for one week. Mice were divided into 10 groups consist of 5 each. Each mouse was infected with Trypanosoma evansi by intraperitonial route. Treatment was given when mice had reached the level of parasitemia 108 – 109 trypanosoma / mL of blood this was predicted 24 hours post-infection (Eisler et al., 2001). The administration of the drug tryapanosidal was done intraperitonial with doses 1mg/kg, 3mg / kg, 5 mg / kg and 7mg / kg. Observation of parasitemia did every 2 times in one week till 60 days of observation. Parasitemia observation was performed using 3 techniques. The first method was native examination used a microscope, if the negative results would be followed by MHCT (Microhaematocrit centrifugation Technique) and BCT (Buffy Coat Technique) according to OIE (2012). Data obtained from the treatment group were the level of parasitemia, the number of deaths and the number of live mice from each test dose. The results are analysed by standard logit or probit. The results of this study showed the effects of the drug Dimianzene aceturate on both isolates varied. On Brebes Isolate was effective at doses of 7 mg / kg BW (100%) and 5mg / kg BW (80%), whereas in the effective dose Pemalang isolate at 3 mg dose / kg BW (80%), 5 and 7 mg / kg BW (100%). While at the lowest dose of 1 mg / kg obtained a level of effectiveness of 0% in both isolates. It could be concluded that both isolates have different pathogens and indicate resistance subpopulation to diminazene aceturate.Keywords : diminazene aceturate, in vivo, resistance, Trypanosoma evansi. 
Case Report Infestasi Psitocobrossus sp pada Macaw (Ara macao) Reza Yesica
Media Kedokteran Hewan Vol. 31 No. 1 (2020): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v31i1.2020.11-22

Abstract

ABSTRAKMacaw termasuk jenis burung yang berukuran besar dan bersuara keras. Dengan nama latin Ara macao, burung ini mempunyai sifat yang lincah, cerdas, dan sangat ramah. Di dunia terdapat 18 spesies macaw, beberapa di antaranya termasuk spesies yang dilindungi. Ektoparasit merupakan salah satu parasit yang banyak ditemukan pada hewan peliharaan dan satwa liar. Salah satu ektoparasit yang sering menyerang burung adalah infestasi kutu. Kutu (lice) memakan bulu dan kulit. Spesies kutu eksklusif dalam kelompok burung beo adalah Psittacobrosus. Kutu ini termasuk dalam keluarga menoponidae. Dalam penelitian ini diambil sampel dari macaw betina berumur 3 tahun yang dibawa ke Rumah Sakit Hewan Pendidikan Universitas Brawijaya. Hasil pemeriksaan menunjukkan bahwa ektoparit yang diduga tergolong Psittacobrosus sp. Pengobatan yang digunakan pada kasus ini adalah dengan Carbaryl 5%, Tetraclorvinphos spray 0,5% serta menjaga kebersihan kandang.
Case Report Leucocytozoonosis pada Kalkun (Meleagris gallopavo) Reza Yesica
Media Kedokteran Hewan Vol. 31 No. 1 (2020): Media Kedokteran Hewan
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mkh.v31i1.2020.45-51

Abstract

ABSTRAKLeucocytozoon adalah parasite genus protozoa yang termasuk dalam filum Apicomplexa yang menginfeksi species unggas. Leucocytozoonosis merupakan salah satu penyakit yang masih sering muncul pada ayam. Angka kematian pada ayam pedaging bisa mencapai 40%. Meskipun kasus penyakit ini lebih banyak ditemukan di peternakan ayam pedaging, bukan berarti ayam petelur dan kalkun dapat lolos dari infeksi parasit ini. Kasus Leucocytozoonosis pada ayam petelur dan kalkun jarang terjadi, namun jika Leucocytozoonosis menyerang, angka kematian bisa mencapai 30%. Hal tersebut juga menyebabkan peningkatan nilai FCR (Feed Conversion Ratio) dan penurunan produksi telur. Dalam penelitian ini diambil sampel darah dari kalkun betina berumur 2 tahun. Hasil pemeriksaan menunjukkan bahwa kalkun terinfeksi protozoa Leucocytozoon sp. Pengobatan pada unggas dapat dilakukan dengan pemberian chloroquine, trimethoprim, sulphaquinoxaline, dan primaquine serta menjaga kebersihan kandang.
Confirmatory Factor Analysis pada Indikator Kesembuhan Pasien Isolasi Mandiri Covid-19 di Indonesia Nur Silviyah Rahmi; Luthfatul Amaliana; Laila Masruro Pimada; Reza Yesica; Devi Nur Cahaya Ningsih
Statistika Vol. 22 No. 1 (2022): Statistika
Publisher : Department of Statistics, Faculty of Mathematics and Natural Sciences, Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/statistika.v22i1.477

Abstract

ABSTRAK Jumlah kasus terkonfirmasi positif Covid-19 di Indonesia pada September 2021 telah mencapai angka 4.204.116 Jiwa dengan pasien meninggal sebanyak 141 ribu orang atau tingkat kematian sebesar 3,4 persen. Kementerian Kesehatan Republik Indonesia melalui Satgas Penanganan Covid-19 telah menerbitkan pedoman pencegahan dan pengendalian Covid-19 untuk menekan angka kematian dan meningkatkan angka kesembuhan. Menurut pedoman tersebut, seseorang yang menjalani isolasi mandiri perlu diberikan perawatan kesehatan, dukungan sosial dan psikososial, serta kebutuhan dasar termasuk makanan, air dan kebutuhan pokok lainnya. Penelitian ini berusaha mengidentifikasi pengaruh faktor eksternal yang meliputi dukungan, serta faktor internal yang meliputi motivasi dan pengetahuan pasien terhadap tingkat kesembuhan penyintas Covid-19 isolasi mandiri. CFA digunakan apabila pengetahuan mengenai struktur suatu faktor laten diketahui. Struktur tersebut diperoleh berdasarkan kajian teoritis, hasil penelitian mengenai hubungan antara variabel yang diobservasi dengan variabel laten. Pendekatan CFA diterapkan untuk mengetahui indikator yang paling besar berpengaruh terhadap variabel laten dukungan sosial, pengetahuan dan motivasi kesembuhan. Pengambilan data primer dilakukan secara online dengan jumlah sampel sebanyak 148 responden penyintas Covid-19 di seluruh Indonesia pada bulan Agustus 2021. Hasil analisis CFA menunjukkan bahwa indikator DS1 (mendapatkan ungkapan empati, kepedulian, dan perhatian yang diberikan petugas terhadap penderita Covid-19) memiliki nilai loading factor terbesar yaitu 0,741. Pada variabel laten pengetahuan, indikator P7 (Tujuan pengobatan Covid-19 adalah menyembuhkan penderita, mencegah penularan dan kematian serta menurunkan tingkat penularan) memiliki nilai loading factor terbesar yaitu 0,767. Sedangkan pada variabel laten tindakan sembuh Indikator TS1 (Bersedia menjalani masa pengobatan dengan pemberian dalam waku pengobatan kurang lebih 14 hari) memiliki nilai loading factor terbesar yaitu 0,924. ABSTRACT The number of positive confirmed cases of Covid-19 in Indonesia in September 2021 has touched 4,204,116 people with the number of patients dying as many as 141,000 people or a death rate of 3.4 percent. The Ministry of Health of the Republic of Indonesia through the Covid-19 Handling Task Force has issued guidelines for the prevention and control of Covid-19 to reduce mortality and increase recovery rates. Based on these guidelines, people who are in self-isolation need to be provided with health care, social and psychosocial support, as well as basic needs including food, water and other basic needs. This study seeks to identify the influence of external factors which include support, as well as internal factors which include the motivation and knowledge of patients on the recovery rate of Covid-19 survivors in self-isolation. CFA can be used when knowledge of the composition or structure of a latent factor is known. The structure is obtained from theoretical studies, the results of research on the relationship between the variables studied and the latent variables. By using the CFA approach, it can be seen that the indicators that have the greatest influence on the latent variables of social support, knowledge and motivation for healing are known as loading factors. Primary data was collected online with 148 COVID-19 survivors across Indonesia in August 2021 as the sample. From the CFA analysis, it was found that the Social Support 1 (DS1) indicator, which is getting expressions of empathy, concern, and attention given by officers to Covid19 sufferers is the largest loading factor value, which is 0.745. This means that the influence of the DS1 indicator on the latent variable of Social Support is 0.745 or 74.5% is influenced by the DS1 indicator compared to the other 4 indicators, namely DS2, DS3, DS4, DS5. In the knowledge latent variable, the Knowledge 7 (P7) indicator, namely the purpose of Covid-19 treatment is to cure the patient, prevent transmission and death and reduce the transmission rate, which is the largest loading factor value, which is 0.767. Then on the latent variable of healing action, Healing Action Indicator 1 (TS1), namely being willing to undergo a treatment period with administration within a treatment time of approximately 14 days) is the largest loading factor value, which is 0.924.