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EFFECT OF SAPONIN-PODS EXTRACT ACACIA (ACACIA MANGIUM) TO HEMATOCRIT, HEMOGLOBIN AT TILAPIA (OREOCHROMIS NILOTICUS) Is Yuniar; Win Darmanto; Agoes Soegianto
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Problem of aquaculture shrimp, one of which is wild fish, competitor. Usually to eliminate the use of saponin from tea seed. saponin to kill fish competitors, (tilapia) are entered when filling water. Saponin to kill fish competitors, (tilapia) are entered when filling water. Saponin used from tea seed meal is imported. Indonesia is rich in biological resources of plants / substances that secondary metabolites naturally untapped optimal. The study was conducted in a laboratory scale with a completely randomized factorial design, using a series of six concentrations and three replicates for each species. LC50 analysis using probit analysis. Unfortunately, there was no scientific information for it. It has been done a laboratory work to study chemical compounds of pod Acacia (Acacia mangium) by phytochemical screening and measure those quantitatively. The result of these were that pod Acacia (Acacia mangium) contain alkaloid, flavonoid, tanin, saponin, steroid, and terpenoid. The results of this study indicate LC50-96h values for Pod Acacia mangium at salinity 0, 5, 10 and 15 promil is 5.487 ppm, 4.313 ppm, 3.985 ppm and 2.944. Value hemoglobin at 0promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 11.353±0.63)g/dL,10.607±0.18g/dL, 9.860±0.36 g/dL and 8.80±0.60. Value hemoglobin at 5promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 8.86±1.02 g/dL,7.76±0.943 g/dL, 7.42±0.71 g/dL and 4.68±0.53g/dL. Value hemoglobin at 10 promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 7.34±1.95 g/dL,6.07±0.755 g/dL, 5.23±1.34 g/dL and 3.23±035 g/dL. Value hemoglobin at 15promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 8.570±0.478 g/dL,5.18±0.876 g/dL, 3.42±0386 g/dL and 3.817±0.355g/dL. Value hematocrit at 0promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 35.033±3.32g/dL, 31.2±0.90 g/dL, 29.367±0.97 g/dL and26.60±1.345. Value hematocrit at 5promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 25.633±1.24 g/dL, 23.90±2.68 g/dL, 18.367±2.554 g/dL and 12.867±2.38 g/dL. Value hematocrit at 10 promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 21.567±6.134 g/dL,18.567±1.48 g/dL,14.767±3.46 g/dL and 10.53±3.38 g/dL. Value hematocrit at 15promil at conentration saponin-pod Acacia 0ppm, 5ppm, 10ppm dan 15ppm consecutive is 26.100±1.42 g/dL, 15.667±2.79 g/dL,9.933± 2.04 g/dL and 11.933±1.19 g/dL. Saponin of acacia may lower hematocrit and hemoglobin an indication of damage to the red blood cells occurs hemolysis.
Renal Protective Effects of Gamma-Mangostin in Streptozotocin-Induced Diabetic Mice Saikhu Akhmad Husen1,2, Salamun1 , Arif Nur Muhammad Ansori3 , Suhailah Hayaza4 , Raden Joko Kuncoro
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 3 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i3.10562

Abstract

This study was aimed to investigate the ability of gamma-mangostin to reduce plasma blood urea nitrogen (BUN) and creatinine and ameliorates the impaired renal proximal tubular cells in diabetic mice. Antioxidant assay was conducted by using male BALB/c mice. Mice were divided into two groups, they were normal control (KN) and streptozotocin-induced diabetic mice. Streptozotocin (STZ) induction was performed using multiple low-dose of 30 mg/kg body weight injected for five consecutive days. Diabetic mice have divided into three subgroups; diabetic control (KD), diabetic mice treated with acarbose (KA), and diabetic mice treated with gamma-mangostin. The gamma-mangostin treatment group was categorized based on the dose given; P1 (1 mg/kg BW), P2 (2 mg/kg BW), and P3 (4 mg/kg BW). Interestingly, gamma-mangostin administration was found to be able to lower plasma BUN and creatinine and ameliorate the impaired renal proximal tubular cells in diabetic mice significantly. Therefore, gamma-mangostin has demonstrated high antioxidant activity. The proof suggests that gamma-mangostin is a lead compound candidate for clinical management or prevent diabetes mellitus.
Effect on Polysaccharide Krestin from Coriolus versicolor Extract on Phagocytic Activity and Capacity of Mus musculus Exposed by Pseudomonas aeruginosa Wahyuningsih, Sri Puji Astuti; Savira, Nadyatul Ilma Indah; Darmanto, Win
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6957

Abstract

Pseudomonas aeruginosa is an opportunistic bacterium that causes infections in human. The wall cell of its bacteria contains lipopolysaccharide as virulency factors to protect it from human immunity. Lipopolysaccharide can inhibit phagocytosis in the body. Polysaccharide krestin (PSK) from Coriolus versicolor extract contains ?-glucan that can increase phagocytic activity and capacity. This research aimed to identify the effect on polysaccharide krestin from C. versicolor extract on phagocytic activity and capacity of mice exposed by P. aeruginosa. The design of this research was experimental design. There were six treatment groups. The phagocytic activity and capacity were counted on slide smears of mice peritoneal fluid. The data was analyzed by using one way ANOVA. The results of the phagocytic activity and capacity showed that PSK was added before exposure (P1) or after exposure (P2) or before-after exposure (P3) had potential to increase phagocytic activity and capacity. The conclusion of the research was that adding polysaccharide krestin either before exposure or after exposure or both of them could increase phagocytic activity and capacity. The benefits of this research to development of science are expected to reduce human infection and to utilize natural ingredients as immunomodulator.How to CiteWahyuningsih, S. P. A., Savira, N. I. I, & Darmanto, W. (2016). Effect on Polysaccharide Krestin from Coriolus versicolor Extract on Phagocytic Activity and Capacity of Mus musculus Exposed by Pseudomonas aeruginosa. Biosaintifika: Journal of Biology & Biology Education, 8(3), 308-313.
Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus Wahyuningsih, Sri Puji Astuti; Pramudya, Manikya; Putri, Intan Permata; Savira, Nadyatul Ilma Indah; Winarni, Dwi; Suhargo, Listijani; Darmanto, Win
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11284

Abstract

Okra (Abelmoschus esculentus) is reported to have various biological functions such as antioxidant, anticancer and anti-inflammation. However, only few studies have been reported immunomodulatory activities of okra to prevent disease caused by bacteria infection. In this study, the immunomodulatory activities of polysaccharides from okra pods were investigated further through the spleen weight and B-lymphocytes proliferation in mice infected by Staphylococcus aureus. Okra polysaccharides were obtained by water extraction and ethanol precipitation. Okra polysaccharides with doses of 25, 50, 75, and 100 mg/kg BW were orally administrated to mice with or without Staphylococcus aureus infection. Spleen weight was evaluated in both treatment group and control group. B-lymphocytes proliferation was evaluated by MTT assay using LPS induction. Results showed that okra polysaccharide at the doses of 50, 75, and 100 mg/kg increased spleen weight (p < 0.05) significantly. While at the dose of 75 and 100 mg/kg, it increased the B-lymphocytes proliferation (p < 0.05) significantly. There is positive correlation between the spleen weight and B-lymphocytes proliferation by 73.3%. These result reveal that okra polysaccharide could improve the immune response and be utilized as a novel candidate of neutraceutical.
ABNORMAL STRUKTUR HISTOLOGIS KORTEKS CEREBELLAR TIKUS DENGAN NORMAL FOLIASI AKIBAT IRADIASI SINAR X MASA POSTNATAL Win Darmanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/453

Abstract

Our previous experiment showed that prenatal exposure of rats to X-irradiation on gestation day 21st as the late gestation period causes heterotopic Purkinje cells and abnormal foliation of the cerebellum. However the present study observed the heterotopik of Purkinje cell layer following exposure of the rats to X-irradiation on postnatal day (P) 4, without abnormal foliation of the cerebellum. It also demonstrated the process of re-derangement of Purkinje cells. Rat pups were exposed to 2.5 Gy X-irradiation and the cerebellum was examined histologically and by immunohistochemistry to identify Purkinje cells. At 6 h after exposure, extensive cell death was observed in the external granular layer (EGL) of the cerebellum. By P7 (3 days after exposure), while Purkinje cells with well developed dendrites aligned underneath the EGL in the control cerebellum, Purkinje cells with shorter and abnormally oriented dendrites failed to align and re-deranged in the heterotopic location. On adult, the cerebellum of rats irradiated was observed almost normal folial developed. It is strong support previous study that the critical stage for cerebellar abnormal foliation caused X-irradiation is in the early postnatal period.
THE EFFECTIVENESS OF HERBAL MOSQUITO COILS “MORIZENA” AGAINST Aedes Aegypti DEATH Rina Priastini Susilowati; Win Darmanto; Nanik Siti Aminah
Indonesian Journal of Tropical and Infectious Disease Vol. 7 No. 2 (2018)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (625.03 KB) | DOI: 10.20473/ijtid.v7i2.7324

Abstract

It has been successfully found new herbal mosquito coils called “Morizena” made from Passiflora foetida leaf extract (40%), Chrysantemum cinerariaefolium flowers seed extract (40%), essential oils of  leaves-stems of Cymbopogon nardus (20%). The herbal mosquito coils are more effective to kill Aedes aegypti compared to commercial synthetic mosquito coils made from Transfluthrin. Treatment of exposure to herbal mosquito coils “Morizena” on Aedes aegypti for 8 hours/day with concentration 500 ppm (P1), 1000 ppm (P2), 2000 ppm (P3), 3000 ppm (P4), 4000 ppm (P5) and treatment of exposure to synthetic mosquito coils Transfluthrin 2500 ppm (K1) as a positive control, and without treatment of exposure to mosquito coils (K0) as a negative control. Experimental animals used were adult Ae. aegypti mosquito with 25 mosquitoes in each treatment. Experimental design used is one-way Anova test with linear regression to calculate its LC50 and LC90. The results of test to herbal mosquito coils “Morizena” given for 8 hours/day obtained mortality of Ae. aegypti by 92% to a concentration of 3000 ppm (P4) and 100% to a concentration of 4000 ppm (P5) and 100% for test to synthetic mosquito coils transfluthrin 2500 ppm (K1). Ae. aegypti LC50 and LC90 value for treatment of exposure to herbal mosquito coils “Morizena” are 999 ppm and 2977 ppm. Treatment of herbal mosquito coils “Morizena” with graded doses up to 4000 ppm and synthetic mosquito coils Transfluthrin 2500 ppm causing an increase in the enzyme acetylcholinesterase activity of Ae. aegypti. The conclusion is based on the Ae. aegypti  LC90 value is 2977 ppm, which means the effective dose of herbal mosquito coils “Morizena” to kill Ae. aegypti is 2977 ppm.
Adverse Effects of Mercury Exposure in DDW Strain Mice during Organogenesis Win Darmanto1 , Saikhu Akhmad Husen2 , Arif Abu Hasan3 , Raden Joko Kuncoroningrat Susilo4, Suhailah
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 4 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i4.12277

Abstract

Mercury (Hg) was known as a teratogenic which is distributed in tissue. This study aims to determine theretention and embryotoxicity of Hg-exposed pregnant mice. Thirty female mice was treated with HgCl2(mercuric chloride). HgCl2 (5 and 6 kg/mg BW) was inducted in pregnant mice at 9 and 11 gestational days.Hg levels were measured in hair, uterus, liver, kidney, brain, blood, placenta, visceral fetus, and fetus brain onthe 18th day of gestation using the atomic absorption spectrophotometry (AAS) method. Embryotoxicity teston the fetus was carried out after the surgery took place. Scales and calipers are used to calculate fetal weightand crown rump length. Statistical tests were analyzed using the SPSS 21 program. The results showed thatthe liver, kidney, brain, visceral fetal, and fetal brain were significantly increased (P < 0.05) in the treatmentgroup at pregnant mice. Hg also produced a significant difference (P < 0.05) on the decrease in live fetuses,fetuses, body weight, and crown rump length and an increase in resorbed fetuses. Hg accumulation in thebody apparently can cause adverse effects in pregnant mice.
INDUKSI 2-METHOXYETHANOL PADA MASA PRENATAL SEBAGAI PENYEBAB KELAINAN OTAK PADA MENCIT Win Darmanto; Eko Prihiyantoro; Ria Harmonis
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/370

Abstract

2-Methoxyethanol (2-ME) and glycol esters are widely used as a solvents in the industry and plasticizers, and have becomes ubiquitous environmental pollutants. This study were designed to evaluate the malformation of brain development as an impact of 2-ME. Four groups of pregnant mice were injected to 2-ME dose 10 mmol/kg body weight at 7, 13, 15, and 17 days gestation respectively. Control group were injected with sterile water. At 18 days of gestation samples in groups 7 and 13 days gestation were sacrificed. Brain were remove and weight, morfologically and histologically were examined. Samples were divided into five groups, i.e. control group, 7th, 13rd, 15th and 17th days of gestation groups. Samples in 7th and 15th days of gestation groups killed in 18th days of gestation to observed fetal brain defect and decreased of fetal brain weight but some samples to allow delivery of fetuses. Furthermore, the fetuses killed on 0, 5, 10, and 20 days after birth, to take their brain. The result of these study showed that there are decreasing total weight of fetal brains and there are cerebral malformation as impact of 2-ME exposure.
Ekspresi Level Gen mRNA Protein Ekstraseluler Otak Embrio Mencit Black-6 UK-12 Akibat Induksi 2-Methoxyethanol : Analisis secara Real Time RT-PCR Yulia Irnidayanti; Win Darmanto; Agus Abadi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/272

Abstract

The aim of this research was to investigate impact of 2-methoxyethanol, a major industrial chemical, and its individual metabolites on the expression DNA of the embryonic brain development of black-6 mice. The expression levels mRNA protein of GAPDH, Fibronectin, tenascin, vimentin, Neurofilamen, NCam between brain embrio treatment with 2-ME at gestation day 12 and Embryo control were achieved. The Electroforesis DNA on brain Embryonic day 12 showed that there were expression of GAPDH (447bp), Fibronectin (462bp), NCAM (293 bp), Tenascin (416bp), Vimentin (327), Neurofilamen high (301bp), Neurofilamen medium (289bp), Neurofilamen low (398bp). This Data not showed. The expression of level of mRNA for protein Vimentin at embryonic brain treatment at GD-12 is 487 copies, meanwhile on the embryonic brain control is 209 copies. This expression is tendency very higher than control. Another level of mRNA for protein fibronectin, NCAM, Tenascin, Neurofilament were tendency not difference between embryonic brain treatments and control. Intermediate filaments, vimentin, is found in specific cell types in the developing and adult central nervous systems (CNS), particularly astrocytes. Recently, found that vimentin immunoreactivities were increased in astrocytes and/or macrophages in the spinal cords of rats with autoimmune inflammation). So that The higher level mRNA for protein vimentin caused by effect 2-methoxyethanol. Vimentin contribute to the repair of brain through the migration of activated cells and increased level vimentin at embryionic brain treatment with 2-ME.
GANGGUAN PEMBENTUKAN ATAP BUMBUNG NEURAL EMBRIO MENCIT AKIBAT INDUKSI 2-ME YANG BERTEPATAN DENGAN MASA NEURULASI PRIMER Eko Prihiyantoro; Win Darmanto; Samekto Wibowo; Mammed Sagi; Sri Kadarsih Soedjono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/365

Abstract

The objective of this study was to determine time of neural fold fusion at dorsal mid line neural axis after treatment 2-methoxyethanol (2-ME) to pregnant mouse during neurulation period and to observe relation process of point of neural fold fusion. Mice at 08:05 gestational days treatment with 2-ME dose 7.5 mmol/kg bw on the other hand control group injected with aqua bidest. Pregnant mice at 08:12, 09:00, 09:12 was sacrificed by dislocation cervix. Embryo was collected after observe with dissecting microscope for external morphology and fixation in Bouin solution and buffer formalin for histological preparation and immunohistochemistry process. Result showed that there was failure of first point neural fold fusion at junction of perspective fore brain and mid brain. The failure of neural fold fusion was caused by increasing apoptosis neuroepithelium. There were no relation process between first point of neural fold fusion and second point or another point of fusion. Failure of first point fusion not cause failure another point of fusion. Observation at 09:12 gestational days showed that the only first point fusion was still open but formation of another part neural tube have finished.