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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Sain Veteriner Jurnal Ilmiah Perikanan dan Kelautan Indonesian Journal of Tropical and Infectious Disease Indonesian Journal of Biotechnology UNEJ e-Proceeding Biosaintifika: Journal of Biology & Biology Education Journal of Aquaculture and Fish Health Indian Journal of Forensic Medicine & Toxicology Berkala Penelitian Hayati Jurnal Kedokteran Hewan
Win Darmanto
Fakultas Sains & Teknologi Universitas Airlangga
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

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KARAKTER PROTEIN ICP11 PADA DNA UDANG VANNAMEI (Penaeus vannamei) YANG TERINFEKSI White Spot Syndrome Virus (WSS V) Yuni Kilawati; Win Darmanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (912.573 KB) | DOI: 10.23869/236

Abstract

The aim of this study was to support policy of government to lessen import and improve export of non migas presented characteristic of genetic of white prawn mains (Penaeus vannamei) so that this product can be yielded in Indonesia with high quality which do not fail with import product. Special goals wishing to be reached at this research is to analyze the genetic characteristic DNA with Polymorphism Chain Reaction (PCR) and primer ICP11. The background of this research was difference among vannamei prawn DNA characteristic that have infected by viruses. With experimental methods ten healthy post larva of white prawn cultured in aquarium and invected by WSSV virus in 1ml/10 l marine water dossage with three time replication during 7 days in culture. To know these infection, the sample were analyze with specific primer ICP11.
Ekspresi Level Gen mRNA Protein Ekstraseluler Otak Embrio Mencit Black-6 UK-12 Akibat Induksi 2-Methoxyethanol : Analisis secara Real Time RT-PCR Yulia Irnidayanti; Win Darmanto; Agus Abadi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/272

Abstract

The aim of this research was to investigate impact of 2-methoxyethanol, a major industrial chemical, and its individual metabolites on the expression DNA of the embryonic brain development of black-6 mice. The expression levels mRNA protein of GAPDH, Fibronectin, tenascin, vimentin, Neurofilamen, NCam between brain embrio treatment with 2-ME at gestation day 12 and Embryo control were achieved. The Electroforesis DNA on brain Embryonic day 12 showed that there were expression of GAPDH (447bp), Fibronectin (462bp), NCAM (293 bp), Tenascin (416bp), Vimentin (327), Neurofilamen high (301bp), Neurofilamen medium (289bp), Neurofilamen low (398bp). This Data not showed. The expression of level of mRNA for protein Vimentin at embryonic brain treatment at GD-12 is 487 copies, meanwhile on the embryonic brain control is 209 copies. This expression is tendency very higher than control. Another level of mRNA for protein fibronectin, NCAM, Tenascin, Neurofilament were tendency not difference between embryonic brain treatments and control. Intermediate filaments, vimentin, is found in specific cell types in the developing and adult central nervous systems (CNS), particularly astrocytes. Recently, found that vimentin immunoreactivities were increased in astrocytes and/or macrophages in the spinal cords of rats with autoimmune inflammation). So that The higher level mRNA for protein vimentin caused by effect 2-methoxyethanol. Vimentin contribute to the repair of brain through the migration of activated cells and increased level vimentin at embryionic brain treatment with 2-ME.
PENGARUH ASAM ASKORBAT TERHADAP KADAR TIMBAL FETUS DAN AKTIVITAS ENZIM SITOKROM P450 1A1 (CYP1A1) PADA INDUK MENCIT TERINTOKSIKASI TIMBAL Juliana Christyaningsih; Harianto Notopuro; Win Darmanto; Diah Titik Mutiarawati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 1 (2010): December 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/280

Abstract

Lead contamination occurs through air pollution and industry, enter the body through respiratory and digestive tract. High lead content will accumulate and affect adversely the cognitive function, causing neuropsychological dysfunction, encephalopathy, hyperactivity and other problems in children, disrupt the central nervous system and the immune system of children as well. This experimental research was randomized control group post-test only design. The experiment used of 27 pregnant mice, divided into three groups: negative control group, which were given distilled water, positive control group were exposed only to lead and the third group were exposed to lead and administered ascorbic acid. 25 mg/kg/day/orally neutral lead acetate was given during gestation day 7 to 16, and ascorbic acid 64 mg/kg/day/orally, started on gestation day 9 to 16. Treatment group with ascorbic acid supplementation had the lowest CYP1A1 enzyme activity compared to positive and negative control groups. This results confirmed by the molecular weight of CYP1A1 enzyme ranges 53.7 to 59.2 kDa, and the western blotting test showed the same thin band both two groups. The lowest of the average lead concentration in the head of fetal mice was found on the group of mice that treated with vitamin C. Supplementation of ascorbic acid can protect the liver and fetuses, by suspected mechanism that ascorbic acid could chelate the lead and excrete it via urine.
GANGGUAN PEMBENTUKAN ATAP BUMBUNG NEURAL EMBRIO MENCIT AKIBAT INDUKSI 2-ME YANG BERTEPATAN DENGAN MASA NEURULASI PRIMER Eko Prihiyantoro; Win Darmanto; Samekto Wibowo; Mammed Sagi; Sri Kadarsih Soedjono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 13 No 2 (2008): June 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/365

Abstract

The objective of this study was to determine time of neural fold fusion at dorsal mid line neural axis after treatment 2-methoxyethanol (2-ME) to pregnant mouse during neurulation period and to observe relation process of point of neural fold fusion. Mice at 08:05 gestational days treatment with 2-ME dose 7.5 mmol/kg bw on the other hand control group injected with aqua bidest. Pregnant mice at 08:12, 09:00, 09:12 was sacrificed by dislocation cervix. Embryo was collected after observe with dissecting microscope for external morphology and fixation in Bouin solution and buffer formalin for histological preparation and immunohistochemistry process. Result showed that there was failure of first point neural fold fusion at junction of perspective fore brain and mid brain. The failure of neural fold fusion was caused by increasing apoptosis neuroepithelium. There were no relation process between first point of neural fold fusion and second point or another point of fusion. Failure of first point fusion not cause failure another point of fusion. Observation at 09:12 gestational days showed that the only first point fusion was still open but formation of another part neural tube have finished.
INDUKSI 2-METHOXYETHANOL PADA MASA PRENATAL SEBAGAI PENYEBAB KELAINAN OTAK PADA MENCIT Win Darmanto; Eko Prihiyantoro; Ria Harmonis
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/370

Abstract

2-Methoxyethanol (2-ME) and glycol esters are widely used as a solvents in the industry and plasticizers, and have becomes ubiquitous environmental pollutants. This study were designed to evaluate the malformation of brain development as an impact of 2-ME. Four groups of pregnant mice were injected to 2-ME dose 10 mmol/kg body weight at 7, 13, 15, and 17 days gestation respectively. Control group were injected with sterile water. At 18 days of gestation samples in groups 7 and 13 days gestation were sacrificed. Brain were remove and weight, morfologically and histologically were examined. Samples were divided into five groups, i.e. control group, 7th, 13rd, 15th and 17th days of gestation groups. Samples in 7th and 15th days of gestation groups killed in 18th days of gestation to observed fetal brain defect and decreased of fetal brain weight but some samples to allow delivery of fetuses. Furthermore, the fetuses killed on 0, 5, 10, and 20 days after birth, to take their brain. The result of these study showed that there are decreasing total weight of fetal brains and there are cerebral malformation as impact of 2-ME exposure.
EFEK 2-METHOXYETHANOL TERHADAP STRUKTUR HISTOLOGI TESTIS MENCIT (Mus musculus) Alifah Hayati; Binti Yunaida; I.B. Rai Pidada; Win Darmanto; Dwi Winarni
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/371

Abstract

This research has done to investigate the effect of 2-Methoxyethanol on the testicular histology of the male mice and also the influence the length of time after administration 2-ME stopped in the recovery of the spermatogenic cells and the diameter also the thicknes of seminiferous tubule. Thirty BALB/C male mice 8–9 week old, weighed 28–30 grams body weight. Those mice separated to 6 groups with 5 male mice each group. Those mice were treated with 2-ME 200 mg/kg body weight daily by intra peritoneal injection, within 3 weeks (K1). To investigate the influence the length of time after administration 2-ME stopped, the male mice after treated by 2-ME in 3 weeks also given by the length of time after 2-ME administration stopped 1, 2, 3 and 4 weeks (P1, P2, P3 and P4). The control animal given by intraperitoneal administration of saline. Histological observation was performed on the number of spermatogonium, primary spermatocyte, oval spermatid and the diameter also epithelial thickness of seminiferous tubules. The data were analyzed by One-Sample T-test to investigate the differences between K0 and K1. One Way ANOVA to investigate the influence the length of time after 2-ME administration stopped in the P1, P2, P3 and P4 and then continuing by LSD (Least Significant Difference) to show the differences groups of treatment. The result showed that administration 2-ME could destroy the seminiferous tubules in the testes. Its presented by the decreasing of the number spermatogonium, primary spermatocyte, oval spermatid and diameter also epithelial thickness of seminiferous tubule. The length of time after administration 2-ME stopped could recover seminiferous tubules condition. Its presented by the increasing of the number spermatogonium, primary spermatocyte, oval spermatid, and diameter also epithelial tickness of seminiferous tubules. The conclution of this research were, 2-ME could destroy the testicular histology of the male mice and the length of time after administration 2-ME stopped have linear correlation with seminiferous tubules recovery.
TINGKAT KELAINAN SEL PURKINJE HETEROTOPIK HUBUNGANNYA DENGAN PERBEDAAN SENSITIVITAS ANTARA LOBUS ANTERIOR DAN POSTERIOR DARI CEREBELLUM TIKUS TERHADAP RADIASI SINAR-X Win Darmanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 2 (2004): June 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/428

Abstract

Our previous experiment showed that prenatal exposure of rats to X-radiation in the late gestation period causes heterotopic Purkinje cells in the internal granular layer (IGL) of the cerebellum. The present study demonstrated precisely the involvement of Reelin in the aligment of Purkinje cells, which are different in severity between anterior and posterior lobes. Pregnant rats were exposed to 2.5 Gy X-radiation on gestation day 21 and the cerebellum of progeny was examined histologically and by immunohistochemistry of IP3 Receptor to identify Purkinje cells. By P8, while Purkinje cells with well developed dendrites aligned underneath the EGL in the control cerebellum, Purkinje cells with shorter and abnormally oriented dendrites failed to align and remained in the heterotopic location in the IGL of the anterior lobes but a small number of heterotopic Purkinje cells formed in the posterior lobes of the irradiated rats. Our previous published Reelin is present in the premigratory zone of the EGL and IGL in the control cerebellum. It decreased in the irradiated cerebellum on P1 to P8, especially the anterior lobes, however in posterior lobes on P8 Reelin level compared to the control (Darmanto, 2003). It is thus indicated that anterior lobes are more sensitive to X-radiation than posterior lobes and the different levels of Reelin between anterior and posterior lobes caused by X-irradiation may contribute the different susceptibility in induction of derangedPurkinje cells.
PENGARUH NIKOTIN TERHADAP KELAINAN TULANG DAN ORGAN INTERNAL MENCIT (Mus musculus) Pieter Kakisina; Win Darmanto; Bambang Poernomo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 9 No 2 (2004): June 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/431

Abstract

This study was designed to identify the effects of nicotine on the skeletal and internal organ abnormalities of mice prenatally. Seventy two female mice strain Balb/C were used. Pregnant mice on gestation days (GD) 8, 10, and 12 was injected intraperitoneally with 3, 6, and 12 mg/kg BW nicotine respectively. Control nice was injected by aguabidestylata. On GD 18, and all of pregnant mice were sacrificed, and a half of living fetuses were immersed in Bouin’s solution to observe their internal organs, while another half were immersed in 95% ethanol to observed sekeletal malformation using alizarin red S staining. Results were analyzed using SPSS program version 11 for personal computer.The results showed that nicotine caused Skeletal malformation, especially in the sternum when administered on GD 8 and 10, in doses of 3 and 6 mg/kg BW, delayed of ossification was observed in the supraoccipital and sacrocaudal bones on GD 10 and 12 and doses of 12 mg/kg BW, while in the extremitas the malformation was observed in the proximal and medial phalanx both in the fore and hind limb especially on GD 10 and 12, and doses of 6 and 12 mg/kg BW. Nicotine was effects on the brain development especially hydrocephalus was observed on GD 8 and doses 10 and 12 mg/kg BW. Another internal malformation such as ectopic renalis was observed on GD 8 and doses of 3 and 6 mg/kg BW. These finding suggested that nicotine has caused skeletal malformation, brain malformatin and ectopic renalis, when administered in mice prenatally.
ABNORMAL STRUKTUR HISTOLOGIS KORTEKS CEREBELLAR TIKUS DENGAN NORMAL FOLIASI AKIBAT IRADIASI SINAR X MASA POSTNATAL Win Darmanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/453

Abstract

Our previous experiment showed that prenatal exposure of rats to X-irradiation on gestation day 21st as the late gestation period causes heterotopic Purkinje cells and abnormal foliation of the cerebellum. However the present study observed the heterotopik of Purkinje cell layer following exposure of the rats to X-irradiation on postnatal day (P) 4, without abnormal foliation of the cerebellum. It also demonstrated the process of re-derangement of Purkinje cells. Rat pups were exposed to 2.5 Gy X-irradiation and the cerebellum was examined histologically and by immunohistochemistry to identify Purkinje cells. At 6 h after exposure, extensive cell death was observed in the external granular layer (EGL) of the cerebellum. By P7 (3 days after exposure), while Purkinje cells with well developed dendrites aligned underneath the EGL in the control cerebellum, Purkinje cells with shorter and abnormally oriented dendrites failed to align and re-deranged in the heterotopic location. On adult, the cerebellum of rats irradiated was observed almost normal folial developed. It is strong support previous study that the critical stage for cerebellar abnormal foliation caused X-irradiation is in the early postnatal period.
Morphometric and Meristic Analysis of Rasbora in East Java Province P Kumaladewi; Mufasirin; Nunuk Dyah Retno Lastuti; Mochammad Amin Alamsjah; Win Darmanto; Sapto Andriyono
Journal of Aquaculture and Fish Health Vol. 11 No. 3 (2022): JAFH Vol. 11 No. 3 September 2022
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v11i3.30956

Abstract

Rasbora (Rasbora sp.) is one of the endemic freshwater fish species in East Java, which is currently being threatened due to environmental degradation, human activities and pollution. In East Java, it is known that there are several species of Rasbora (Rasbora sp.). Domestication is one method to preserve Rasbora in East Java, preceded by identifying potential species of Rasbora sp. from the natural catchment. This study aims to determine the diversity of Rasbora sp in East Java. Samples were taken at five different locations, specifically: Nganjuk (Brantas Watersheds), Ngawi (Bengawan Solo Watersheds, Silowo Springs Tuban, Umbulan Springs Pasuruan and the Setail Watersheds Banyuwangi. The main parameters observed were morphology and morphometry. The morphological analysis used identification guidelines, while morphometric data were obtained through 15 parameters measurement then analyzed using one-way Anova test on SPSS 25 software. Based on the morphological and morphometric analysis, it is known that the species of Rasbora in Nganjuk and Ngawi regencies are Rasbora argyrotaenia and the species of Rasbora in Tuban, Pasuruan and Banyuwangi regencies are Rasbora lateristriata. One-way Anova test showed Rasbora from the five locations were significantly different based on the result value of p<0.05.
Co-Authors Agoes Soegianto Agus Abadi Alifah Hayati Andita Ayu Mandasari Aulanni'am, Aulanni'am Bambang Poernomo Betti Lukiati Binti Yunaida Diah Titik Mutiarawati Djoko Agus P. Dwi Winarni Eko Prihiyantoro Eko Prihiyantoro Endang Dewi Masithah Harianto Notopuro I.B. Rai Pidada Intan Permata Putri Is Yuniar Juliana Christyaningsih Lailatul Lutfiyah, Lailatul Listijani Suhargo, Listijani Mammed Sagi Manikya Pramudya Mochammad Amin Alamsjah Mufasirin Nadyatul Ilma Indah Savira Nanik Siti Aminah Natalia Tri Astuti Nunuk Dyah Retno Lastuti Nurmalasari Darsono P Kumaladewi Pieter Kakisina Ria Harmonis Rr. Juni Triastuti Samekto Wibowo Sapto Andriyono Sri Kadarsih Soedjono Sri Puji Astuti Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Sukardiman Susilowati, Rina Priastini Suvia Widyaningrum Tri Dewanti W. Widhi Dyah Sawitri YULIA IRNIDAYANTI Yuni Kilawati