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All Journal Jurnal Perikanan dan Kelautan BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Matematika Sains dan Teknologi ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education FITOFARMAKA: Jurnal Ilmiah Farmasi
latifah K Darusman
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology Mathematics Medicine & Pharmacology Social Sciences Other

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PROSES NITRIFIKASI OLEH KULTUR MIKROBA PENITRIFIKASI N-Sw DAN ZEOLIT Agustiyani, Dwi; Imamuddin, Hartati; Gunawan, Edi; Darusman, Latifah K
BERITA BIOLOGI Vol 8, No 5 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.491 KB) | DOI: 10.14203/beritabiologi.v8i5.1904

Abstract

The addition of zeolite into the mix culture of nitrifying bacteria N-Sw was investigated in order to improve the nitrification activity. In this experiment, the ammonium conversion was investigated by zeolite as a sole agent and also mixed with nitrifying culture N-Sw.The mix culture of nitrifying bacteria N-Sw was developed from the sludge of wastewater treatment of palm oil industry, which acclimated by ammonium sulfate for about one year. The result show that the nitrification efficiency on the treatment using nitrifying culture N-Sw was 30.76%, the ammonium elimination rate was 7.46 mg N-NH /L/hour.The addition of 10 g/l zeolite, increase both the nitrification efficiency (64.58%), and the ammonium elimination rate (14.0 mg N-NH /L/hour).The nitrification efficiency increased to be 100% on the second and third day operation, and the ammonium elimination rate was increased to be 22.4–22.9 mg N-NH /L/hour. From this experiment indicated that the role of zeolite on the improving the nitrification activity was as an absorbent of ammonium.
Relationship between Zingiberaceae Leaves Compounds and its Tyrosinase Activity Batubara, Irmanida; Kartika, Yuni; Darusman, Latifah K
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6742

Abstract

The leaves of Zingiberaceae family has not been much explored its potential, especially as a skin lightening. The relationship between total anthocyanins, chlorophyll, carotenoids and tannins contents of Zingiberaceae leaves and tyrosinase activity were determined. Ten species of Zingiberaceae were separated by n-hexane and the residues were extracted with ethyl acetate. The total anthocyanins, chlorophyll, carotenoids, tannins contents and the activities of ethyl acetate extracts were determined by spectrometric method. The tyrosinase inhibition was determined by using L-tyrosine (monophenolase) and L-DOPA (diphenolase) substrates. The most active extract was the leaves extract of Zingiber purpureum which exhibited 82.86% for monophenolase inhibition and the leaves extract of Curcuma zedoaria which exhibited 90.20% for diphenolase inhibition. The correlation between carotenoids content and monophenolase inhibition was 52%, while between tannin content and diphenolase inhibition was only 15%. Therefore, among 10 leaves species of Zingiberaceae, Zingiber purpureum Roscoe and Curcuma zedoaria leaves are the most potential for tyrosinase inhibitors and can be developed as whitening agent.How to CiteBatubara, I., Kartika, Y. & Darusman, L. K. (2016). Relationship between Zingiberaceae Leaves Compounds and its Tyrosinase Activity. Biosaintifika: Journal of Biology & Biology Education, 8(3), 371-377.
Cellulolytic Yeast Isolated From Raja Ampat Indonesia Kanti, Atit; Sukarno, Nampiah; Sukara, Endang; Darusman, Latifah K
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.042 KB) | DOI: 10.1234/46

Abstract

The objective of this study was to select and characterize three yeast isolates originating from soil of  Raja Ampat region of Papua, Indonesia for its potential to produce cellulase . Selection and characterization of cellulolytic yeast was carried out by measuring cellulolytic Index (IS) with congo red method and measurement of Carboxy Methyl Cellulase (CMC-ase) activity through determination of reducing sugar with dinitrosalycilic methods. Cellulolytic Index (IS) of the isolates  Sporobolomyces poonsookiae Y08RA07,  Rhodosporidium paludigenum Y08RA29 and Cryptococcus flavescens Y08RA33were 1.40, 2.60 and 1.66 respectively. CMC-ase produced optimum at pH 8 at 37ºC by isolate Y08RA07, whereas for Y08RA29 andY08RA33 were at pH 6, at 28ºC. Paper waste was good substrate for cellulase enzyme production by isolate Y08RA07, while for two other isolates the best substrate was CMC. Isolate Y08RA29 having highest cellulase activities when grown in CMC, while isolates Y08RA07 and Y08RA33 achieved highest enzyme activity when grown in bamboo leaf.   Key words: Cellulolytic yeast, Raja Ampat, waste paper, bamboo leaf
PENGOLAHAN LINDI SEBAGAI PUPUK CAIR UNTUK MENDUKUNG PENGEMBANGAN TPA SAMPAH LESTARI Nurhasanah, Nurhasanah; Darusman, Latifah K; Sutjahjo, Surjono Hadi; Lay, Bibiana Widiati
Jurnal Matematika Sains dan Teknologi Vol 11 No 1 (2010)
Publisher : LPPM Universitas Terbuka

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

A laboratory scale research to obtain environmentally safe effluent and liquid fertilizer from leachate from Final Waste Disposal Site in Galuga owned by Regional Government of Bogor City was conducted from July 2006 through to April 2007. The experiment was initiated by aerating the leachate in 4 difference aeration rates (0, 10, 30 and 70 liters/minute) followed by processing the sediment to product the liquid fertilizer. The experiment of production liquid fertilizer was carried out by adding lime with different dosage into sediment generated from processing by aerating at 70 liters/minute followed by centrifugation process or secher. Further, the liquid fertilizer generated from such experiment was applied to chilis planting (Capsicum annum). The research found that the processing conducted by aerating at the rate 70 liters/minute was the most effective in reducing pollutant from leachate. The addition of 1000 ppm CaO or Ca(OH)2 limes in sediment from aeration is the most effective in depositing the dissolved material compared to the addition of limes in other dosage. Liquid fertilizer generated through the addition of 1000 ppm CaO have the content of N = 375,83 ppm, P = 121,44 ppm, K = 948,11 ppm, Ca = 827,20 ppm, Mg = 959,50 ppm, S = 48,53 ppm, Cu = 8,23 ppm, Zn = 30,02 ppm, Mn = 230,57 ppm, Fe = 320,95 ppm, Pb = 10,34 ppm, Cd = 7,46 ppm and Cr = 2,05 ppm. The use of liquid fertilizer generated by adding 1000 ppm CaO was the most effective in enhancing vegetation growth and production of chili. The non essential elements (Pb, Cd and Cr) in fruits from vegetation given liquid fertilizer produced from such treatment did not exceed tolerable threshold.
AKTIVITAS EKSTRAK TEMULAWAK (Curcuma xanthorrhiza Roxb.) TERHADAP PROLIFERASI DAN DIFERENSIASI SEL OTAK BESAR ANAK TIKUS BERUMUR TIGA HARI SECARA IN VITRO Rahminiwati, Min; Juwita, Ita; Murtisari, Ani; Darusman, latifah K
FITOFARMAKA | Jurnal Ilmiah Farmasi Vol 1, No 2 (2011): FITOFARMAKA
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.734 KB)

Abstract

Kurkumin yang terdapat dalam rimpang temulawak, selain dapat menginduksi terjadinya proliferasi sel progenitor pada otak tikus dewasa juga dapat menghambat kerja enzim tirosinkinaseyang berperan penting dalam mengatur pertumbuhan dan diferensiasi sel. Meskipun demikianrespon sel saraf terhadap ekstrak temulawak pada masa pertumbuhan perlu kajian lebih lanjut. Efekekstrak temulawak terhadap proliferasi dan diferensiasi sel otak besar atau serebrum pada masapertumbuhan anak diteliti pada sel otak anak tikus Spague Dawley berumur tiga hari yangditumbuhkan dalam media DMEM (Dulbecco’s Modified Eagle’s Medium). Perlakuan dikelompokkan dalam kelompok kontrol positif (mDMEM+30 µg/mL asiaticoside (AC), kontrolnegatif (mDMEM), kelompok yang memperoleh ekstrak temulawak (CZ) 100 ppm (mDMEM+100ppm CZ), CZ 200 ppm (mDMEM+200 ppm CZ), dan CZ 400 ppm (mDMEM+400 ppm CZ). Kultur diinkubasi pada suhu 37 o C dalam inkubator CO 5 % selama 6 hari. Parameter yang diamatiadalah population doubling time, komposisi sel saraf dan sel glia, panjang akson dan dendrite yangdiukur masing masing menggunakan hemositometer, pewarnaan Hematoxyilin Eosin (HE) danmikrometer. Hasil penelitian menunjukkan ekstrak temulawak pada konsentrasi 100 ppmmemperlambat prolfperasi, pada konsentrasi 400 ppm meningkatkan diferensiasi sel yangditunjukkan dengan meningkatnya ratio sel glia terhadap sel saraf dan mempengaruhi panjangakson dan dendrite.Kata kunci : Curcuma xanthorrhiza Roxb., neuron, sel glia, dendrite 
AKTIVITAS INSEKTISIDA BAGIAN TUMBUHAN CALOPHYLLUM SOULTTRI BURM.F. (CLU IACEAE) TERHADAP LARVA LEPIDOPTERA Syahputra, Edy; Manuwoto, Syafrida; Darusman, Latifah K; ., Dadang; Prijono, Djoko
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 4, No 1 (2004): Maret, Jurnal Hama dan Penyakit Tumbuhan Tropika
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1953.917 KB) | DOI: 10.23960/j.hptt.1423-31

Abstract

 The objective of this test was evaluate the insecticidal activity of ectract of some parts of Calophyllum soulattri (Clusiaceae) against larvae of three species of Lepidoptera, i. e Crocidolomia pavonana, Plutella xylostela, and Pieris sp.. Extraction of plant materials was done by infusion method using ethanol. The bioassays were conducted by leaf-feeding method. Second-instar larvae were fed extract-treated broccoli leaves of 48 hours, then they were presented with untreated leaves until the surviving larvae larvae reached the fourth-instar stage. The number of dead larvae was recorded daily an larval mortality date were analyzed by probit method. The result showed the gummy bark exudates and bark extract of old and young C. soulattri plants were highly active against C. pavonana. The abrk extact of old C. soulattri plant was also effective against P. xilostella and Pieris sp. The gummy exudates possessed strong insecticidal activity against C. pavonana larvae with LC50 of 0.04% and prolonged the developmental time from second to fourth instar of C. soulattri 2.03-7.25 days compared with control. The bark excudate gave positive respon to alkaloid flavonoid, and tannin test. Futher studies are needed to identify insecticidal compound in those active extracts.
ANAL1SIS BEBAN PENCEMARAN, KAPASITAS ASIMILASI DAN TINGKAT PENCEMARAN DALAM UPAYA PENGENDALIAN PENCEMARAN PERAIRAN DANAU MANINJAU Marganof, Marganof; Darusman, Latifah K; Riani, Fain; Pramudya, Bambang
Jurnal Perikanan dan Kelautan Vol 12, No 01 (2007)
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jpk.12.01.8-14

Abstract

Water quality in Maninjau Lake has been thereatened by organic and inorganicmatters that are flown into the lake. The pollution sources arc many activitiesaround the lake such as residential area, husbandry, agriculture, an fish culture onthe lake (floating net cage). These activities result in huge pollution laods and thetrends are predicted to increase. Understending on the pollution loads, polluitonsources and asssimilativc capacity are important in formulating pollution controltechniques in lake waters. The objectives of the research was to determine level ofpollution waters of Maninjau Lake. The results of this research showed thatpollution load parameter susch as Total Suspended Solid (TSS), ChemicalOxygen Demand (COD), Biochemical Oxygen Demand (BOD) and TotalPhosphorus were very high. All parameters of pollution loads have ciceded itsasssimilative capacity. The level of pollution in the study site is classified asmedium category.
Co-Authors Atit Kanti Bambang Pramudya BIBIANA W LAY Dadang . DJOKO PRIJONO Dwi Agustiyani Edi Gunawan EDY SYAHPUTRA Endang Sukara, Endang Fain Riani Hartati Imamuddin Irmanida Batubara Juwita, Ita Marganof, Marganof Min Rahminiwati Murtisari, Ani Nampiah Sukarno Nurhasanah Nurhasanah Surjono Hadi Sutjahjo SYAFRIDA MANUWOTO Yuni Kartika, Yuni