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Efektivitas Air Rebusan Daun Binahong (Anredera cordifolia) Terhadap Pertumbuhan Salmonella typhi Ratih Dewi Dwiyanti; Nurlailah Nurlailah; Indah Kurnia Widiningsih
Medical Laboratory Technology Journal Vol 1, No 1 (2015): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Typhus is one of acute febrile illness caused by the bacterium Salmonella typhi. Treatment of typhoid fever usually use antibiotics, the use of antibiotics can cause side effects. People today are using treatment with natural ingredients, one of which is Binahong (Anredera cordifolia) compounds containing alkaloids, polyphenols, flavonoids, saponin, and anthraquinone is efficacious as an antibacterial. This study aims to determine the effectiveness of the water decoction of leaves Binahong against Salmonella typhi growth in vitro. This type of research is true experiment with posttest study design Only Control Group Design and methods used are diffusion (wells) with 5 treatment. The concentration of the cooking water leaves the dgunakan Binahong is 20%, 40%, 60%, 80% and 100%. The result showed inhibition zone water decoction of the leaves Binahong against Salmonella typhi at a concentration of 20%, 40%, 60%, 80% is 0 mm, whereas at 100% concentration obtained inhibition zone of 11 mm. It is concluded that the water decoction of the leaves Binahong at a concentration of 100% has the ability to inhibit the growth of Salmonella typhi, but these results have not been effective because it is still in the category of resistance. It is suggested for further research to increase the concentration of water decoction of the leaves binahong or use alcohol extract of leaves binahong to inhibit the growth of Salmonella typhi.

Daya Bunuh Ekstrak Air Daun Salam (Syzygium polyanthum) terhadap Larva Aedes sp Ratih Dewi Dwiyanti; Rion Dediq; Anny Thuraidah
Medical Laboratory Technology Journal Vol 3, No 1 (2017): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

edes sp is known as a mosquito that transmits various diseases. In addition to spreading dengue fever and chikungunya, these mosquitoes also spread the virus zika is quite dangerous. One of the effort to controlling these diseases is to eradicate the vector by using materials that can be taken from plants one of them is bay leaf (Syzygium polyanthum). The purpose of this study is to determine the kills power of Syzygium polyanthum water extract against Aedes sp larvae. This research uses true experiment with Posttest Only With Control Group Design design. Data were obtained from the number of larval deaths in the Syzygium polyanthum extract in each control group and treatment group for 24 hours. The results showed that the percentage of bayleaf water extract which has the largest number of larval deaths was at 32% concentration with 55% death percentage. It was concluded that each concentration of aqueous bay leaf water extract given had a killing power against Aedes sp larvae with the lowest percentage of 2.5% and the highest 55%. Based on the result of Kruskal-Wallis test showed that water extract of the bay leaf was able to kill Aedes sp larvae at each treatment with the value of sig 0,001. Suggestion for the next researcher to test the power of killing the water extract of Syzygium polyanthum to Aedes sp larvae by increasing the concentration of salted bay water extract.

Lime Soap as Bacteria Reducer Ratih Dewi Dwiyanti; Leka Lutpiatina
Medical Laboratory Technology Journal Vol 4, No 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Some people in South Kalimantan have used river water to fulfill their daily needs include washing the utensils. Wash the cutlery use river water can cause disease transmission. Squeezed lime has an antibacterial effect in inhibiting bacterial growth. Antibacterial dishwashing soap is effective in reducing pathogenic bacteria. This research is to know the difference of bacterial amount on the variation of contact time with soap. Know the difference of bacteria on the variation of concentration of lime soap and soap without lime. The study used the experimental method with pretest and posttest with control design. The independent variables are a lime soap with a concentration of 40%, 50%, 60% and variable of contact time 30 second, 60 second and 90 seconds. The dependent variable is the number of bacteria on the dinnerware of a dinner plate. The data obtained in this study is the data of measurement of the number of bacteria on the dinner plate. Data analyzed by Kruskal-Wallis test. MPN coliform examination of river water showed results >2400 CFU/ml sample. There were no significant differences in treatment with contact time variation of 30 seconds, 60 seconds and 90 seconds. The significance value is 0.796 (> 0.05). There were significant differences in the treatment of lime soap with concentration variations of 40%, 50%, 60%, and soap without lime. The value of significance is 0,000 (<0.05).

MRSA dan VRSA pada Paramedis RSUD Ratu Zalecha Martapura Ratih Dewi Dwiyanti; Akhmad Muhlisin; Akhmad Muntaha
Medical Laboratory Technology Journal Vol 1, No 1 (2015): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Abstact: Staphylococcus aureus strains isolated from hospital generally been resistant to many antimicrobial-resistant strains of Staphylococcus aureus to vancomycin was rarely reported. Strains of Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infections that are multiresistant to antibiotics. This study aims to determine the presence of bacteria Methicillin-resistant Staphylococcus aureus (MRSA) and Vancomycin resistant Staphylococcus aureus (VRSA) on paramedics in the treatment room and the surgical ICU care hospitals Zalecha Martapura queen. This study used a descriptive survey method. The population in this study were nurses who worked in the surgical and ICU care that numbered 38 people. Sampling technique accidental sampling with a sample of 27 people. The survey results revealed 27 positive samples of Staphylococcus aureus 14 people, there is a sensitivity test results of 7 samples were resistant to cefoxitin (MRSA 26%), 4 samples were resistant to oxacilin and 7 samples were resistant to vancomycin (VRSA 26%). Concluded there is MRSA and VRSA in hospital Queen zalecha Martapura, it is advisable to increase the cleanliness of individuals and the use of personal protective equipment to reduce the incidence of nosocomial infections in the scope of the hospital.

Staphylococcus aureus pada keyboard di unit pelayanan penunjang rumah sakit Deby Arnisya Putri; Erpan Roebiakto; Ratih Dewi Dwiyanti
Jurnal Skala Kesehatan Vol 9 No 1 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Computer keyboard can be a cause of health problems due to poor cleanliness. Keyboard that is not cleaned contains more bacteria. Staphylococcus aureus is one of the bacteria that causes nosocomial infection that can be found on the keyboard. The purpose of this research is to know the description of Staphylococcus aureus bacteria in Hospital Supporting Unit. This type of research is descriptive survey with 16 samples with total sampling. Based on research conducted on the keyboard in the Medical Support Unit Hospital found Staphylococcus aureus as much as 68.75% It is recommended to do keyboard cleanliness and cleanliness of computer users before and after using the computer.

Angka Kuman Es Batu Produksi Rumah Tangga Annisa Nur Yulianti; Ratih Dewi Dwiyanti; Wahdah Norsiah; Leka Lutpiatina
Jurnal Skala Kesehatan Vol 9 No 1 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Air adalah kebutuhan dsar manusia, baik untuk keperluan sehari-hari seperti mandi, cuci, masak, dan minum. Air tidak hanya untuk air minun saja, tetapi dapat dibuat es batu yang memenuhi syarat bakteriologis. Penelitian bertujuan untuk mengetahui hasil TPC (Total Plate Count) pada es batu produksi rumah tangga di Kelurahan Sungai Besar. Penelitian ini bersifat survei deskriptif, sampel diperiksa dengan menggunakan metode TPC (Total Plate Count) dengan pengenceran 100 sampai 10-4. Sampel pemeriksaan berupa es batu yang diambil dari rumah produksi di Kelurahan Sungai Besar yang berjumlah 6. Hasil penelitian dari 24 sampel es batu produksi rumah tangga di Kelurahan Sungai Besar adalah 2 sampel yang memenuhi syarat dan 22 sampel tidak memenuhi syarat. Kesimpulan dari penelitian ini adalah 80% sampel tidak memenuhi syarat Standar Nasional Indonesia (SNI) 01-3553-2006. Saran penelitian lebih lanjut dengan melakukan identifikasi terhadap jenis bakteri yang terdapat dalam es batu tersebut.

DAYA HAMBAT EKSTRAK DAUN KENIKIR (Cosmos caudatus Kunth.) TERHADAP Staphylococcus aureus Leka Lutpiatina; Nur Rizqi Amaliah; Ratih Dewi Dwiyanti
Meditory : The Journal of Medical Laboratory Vol 5, No 2 (2017): meditory ,Volume 5, No 2, Desember 2017
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Daun kenikir (Cosmos caudatus Kunth.) sudah lama dimanfaatkan masyarakat untuk dikonsumsi maupun pengobatan tradisional. Daun kenikir memiliki kandungan fenol, flavonoid, saponin dan tanin yang berfungsi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui konsentrasi hambat minimal dan konsentrasi bunuh minimal ekstrak daun kenikir terhadap bakteri Staphylococcus aureus secara in vitro. Penelitian ini bersifat true eksperiment dengan rancangan postest only control group design. Variabel bebas dalam penelitian ini adalah konsentrasi ekstrak daun kenikir (Cosmos caudatus Kunth.). Variabel terikat dalam penelitian ini adalah bakteri Staphylococcus aureus. Hasil penelitian Konsentrasi Hambat Minimal (KHM) menunjukkan kekeruhan pada konsentrasi 160 mg/ml, 320 mg/ml dan adanya kejernihan pada konsentrasi 340 mg/ml, 380 mg/ml dan 400 mg/ml. Hasil penelitian Konsentrasi Bunuh Minimal (KBM) diperoleh jumlah koloni pada konsentrasi 160 mg/ml sebanyak 60 CFU/ml, 320 mg/ml sebanyak 12 CFU/ml, 340 mg/ml sebanyak 3 CFU/ml, 380 mg/ml sebanyak 0 CFU/ml dan 400 mg/ml sebanyak 0 CFU/ml. Berdasarkan hasil penelitian dapat disimpulkan bahwa KHM ekstrak daun kenikir adalah 340 mg/ml dan KBM ekstrak daun kenikir adalah 380 mg/ml. Disarankan dilakukan penelitian lebih lanjut mengenai pengaruh ekstrak daun kenikir terhadap bakteri Staphylococcus aureus adalah dengan menggunakan pelarut lain.

Improved Mycology Media Formula of Corn and Cow’s Hair For Accelerate Superficial Mycosis Growth Ratih Dewi Dwiyanti; Leka Lutpiatina
Medical Laboratory Technology Journal Vol 7, No 2 (2021): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v0i0.391

Potato Dextrose Agar is growth and identification media of fungi that cause superficial mycoses, including Trichophyton rubrum and Trichophyton mentagrophytes with 10–14 days growth period. Modification media is needed to accelerate the growth of the fungus. Zea mays saccharata has a high carbohydrate, and cow’s hair contains high keratin, which can be used as a nutrient to replace fungal growth media. The study aimed to obtain a modified media formula containing Zea mays saccharata as a substitute for dextrose and cow's hair as a source of keratin to accelerate the growth of Trichophyton rubrum and Trichophyton mentagrophytes fungi. The modified media formula is mixing of Zea mays saccharata 15% to 25%, and cow's hair 20mg to 35mg. Acceleration of growth time based of when found microconidia and macroconidia, diameter measurements, and observations of texture and colonies color of Trichophyton rubrum and Trichophyton mentagrophytes. The study results are Zea mays saccharata media with the addition of cow hair for Trichophyton rubrum grew 11 days with 10%+20mg formula and grew only seven days with 25%+35mg formula. Modified media for Trichophyton mentagrophytes grew seven days for 10%+20mg formula, while it was only three days with 25%+35mg formula. The media formula with the fastest growth rate for Trichophyton rubrum and Trichophyton mentagrophytes was at a concentration of 25%+35mg. The Advanced Modified Media can become a patent medium for faster growth of superficial fungi at a lower cost. Further research needs to be done on how long this media can be stored for long-term use.

Efektivitas Air Perasan Jeruk Nipis (Citrus aurantifolia) dalam Menghambat Pertumbuhan Escherichia coli Ratih Dewi Dwiyanti; hana nailah; Ahmad Muhlisin; Leka Lutpiatina
Jurnal Skala Kesehatan Vol 9 No 2 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Lime (Citrus aurantifolia) can be used for cough medicine, sputum laxative, influenza, and acne medication. Lime contains useful chemical compounds, one of which is essential oils and flavonoids which function as antibacterials and play a very important role in inhibiting bacterial growth. The purpose of this study was to determine the effectiveness of lime juice (Citrus aurantifolia) at a concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% on the growth of Escherichia coli in vitro. This research is experimental with post test only control group design. The research sample was lime juice. Antibacterial power testing using well diffusion method. Antibacterial power parameters were determined by measuring the inhibitory zone (mm) formed around the growth of test bacteria on Muller Hinton Agar media. The results of the study showed that lime juice inhibition zones against the growth of Eschericia coli at concentrations of 40%, 50%, 60%, 70%, 80%, 90% and 100% were 7.25mm, 13.25mm, 14.25mm, 16mm, 17mm, 18.25mm, and 20.75mm. Based on linear regression test obtained significant value of 0.000 <α 0.05 so it can be concluded that there is an effect of lime juice on Eschericia coli growth in vitro. It is recommended that further research be carried out to test the inhibitory power of lime juice using other methods or against bacteria of other species.

Pengaruh Lama Penyimpanan Jamu Beras Kencur terhadap Pertumbuhan Kapang Fransiskus Xaverius Agung Pinto Laksono; Ratih Dewi Dwiyanti; Rifqoh Rifqoh; Aima Insana
Jaringan Laboratorium Medis Vol 4, No 2 (2022)
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i2.8571

During the Covid-19 pandemic, people began to maintain their health, by using herbal medicine as an alternative treatment. One type of herbal medicine used is jamu beras kencur because it has properties to increase stamina, increase appetite, and relieve aches and cough disorders. In the Regulation of the Minister of Health of the Republic of Indonesia No. 007 of 2012 carrying herbs such as jamu beras kencur does not require marketing authorization. The quality of the herbal medicine produced has not been confirmed as feasible from microbial contamination, especially in molds, the number of yeast molds in the jamu beras kencur must comply with BPOM standards Number 32 of 2019 namely AKK 103 colonies/mL. This study aimed to determine the effect of the storage time of jamu beras kencur on mold growth with storage treatments of 0 hours, 4 hours, 8 hours, 12 hours, and 24 hours at room temperature. This type of research is a Quasi Experiment with a research design of One Group Pretest-Postest Design. The results of this study showed that the average AKK value was 0 hours 0.2x100 colonies/mL, 4 hours 0.4x100 colonies/mL, 8 hours 0.4x100 colonies/mL. 12 hours 0.8x100 colonies/mL and 24 hours 2.2x100 colonies/mL. These results stated that the jamu beras kencur met the BPOM standard requirements Number 32 of 2019 which was AKK 103 Colonies/mL. The results of the Kruskal Wallis test obtained a sig value of 0.286, it was concluded that there was no effect of storage time for the jamu beras kencur on the growth of molds. The identification results on the jamu beras kencur obtained fungal species, namely Aspergillus flavus, Aspergillus niger, and Penicillium sp. Suggestions for further researchers can examine the effect of storage time on mold growth in other types of herbal medicine.

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