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Pewarnaan Gram Buffy Coat untuk Deteksi Awal Pasien Bakteremia leka lutpiatina
Medical Laboratory Technology Journal Vol 1, No 1 (2015): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (871.726 KB) | DOI: 10.31964/mltj.v1i1.15

Abstract

Abstract: Bacteremia is one public health problem. Bacteremia was ranked as the tenth leading cause of death in the United States. Gram staining of buffy coat can be used for early detection of patients with bacteremia. Rapid diagnostic tests with these Gram staining can be interpreted within an hour and the research that has been conducted Richmond et al. 2002, have a sensitivity of 75% and specificity of 79%. This study aimed to study the sensitivity and specificity of Gram buffy coat with the gold standard blood culture media biphasic fever suspected patients. This study was an observational study with cross sectional study. The study population was suspected fever patients in hospitals Ratu Zalecha Martapura months from March to May 2013. The samples were suspect fever patients in hospitals Queen Zalecha Martapura March-May 2013 were taken by purposive with the inclusion criteria: Patients District General Hospital Ratu Zalecha Martapura, aged ≥10 years, had fever ≥ 5 days, had symptoms of abdominal pain, willing to follow the study. Gram staining microscopic results were analyzed by application epicalc. Microscopic results buffy coat Gram staining Gram-negative rods are found as many as seven (26%) and were not found Gram-negative rods as many as 20 (74%). Culture results of this study are Gram negative bacterial growth as much as 6 (22%) and no growth as many as 21 (78%). Conclusion The results of microscopic Gram stain buffy coat has a value of 67% sensitivity and 86% specificity.
Lime Soap as Bacteria Reducer Ratih Dewi Dwiyanti; Leka Lutpiatina
Medical Laboratory Technology Journal Vol 4, No 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1039.247 KB) | DOI: 10.31964/mltj.v4i1.181

Abstract

Some people in South Kalimantan have used river water to fulfill their daily needs include washing the utensils. Wash the cutlery use river water can cause disease transmission. Squeezed lime has an antibacterial effect in inhibiting bacterial growth. Antibacterial dishwashing soap is effective in reducing pathogenic bacteria. This research is to know the difference of bacterial amount on the variation of contact time with soap. Know the difference of bacteria on the variation of concentration of lime soap and soap without lime. The study used the experimental method with pretest and posttest with control design. The independent variables are a lime soap with a concentration of 40%, 50%, 60% and variable of contact time 30 second, 60 second and 90 seconds. The dependent variable is the number of bacteria on the dinnerware of a dinner plate. The data obtained in this study is the data of measurement of the number of bacteria on the dinner plate. Data analyzed by Kruskal-Wallis test. MPN coliform examination of river water showed results >2400 CFU/ml sample. There were no significant differences in treatment with contact time variation of 30 seconds, 60 seconds and 90 seconds. The significance value is 0.796 (> 0.05). There were significant differences in the treatment of lime soap with concentration variations of 40%, 50%, 60%, and soap without lime. The value of significance is 0,000 (<0.05).
Produk Urikase dari Bacillus sp. Kontaminan Laboratorium leka lutpiatina
Medical Laboratory Technology Journal Vol 1, No 2 (2015): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (725.899 KB) | DOI: 10.31964/mltj.v1i2.22

Abstract

Urease is an enzyme catalyst in the reaction of oxidation of uric acid into Allantoin. Oxidation of uric acid by the enzyme has become a basic principle of measurement of uric acid levels in the human body. This research to know urikase derived from the bacterium Bacillus sp that contaminate the air Laboratory of Microbiology Department of Health Analyst Banjarmasin. The research is descriptive survey. Airborne bacteria were isolated and identified for Bacillus sp. The identification is done by examination of macroscopic, microscopic and biochemical tests subsequently performed tests using nutrient agar medium containing 0.2% of uric acid. Results of the study showed there were two isolates of Bacillus sp and obtained clear zone on both these isolates. Conclusions of research there are contaminants Bacillus sp. as much as 40% in the laboratory. 100% of the yield urikase Bacillus sp. Urikase advice from Bacillus sp can be used as an alternative reagent uric acid probes spectophotometric method.
Pengaruh Variasi Suhu Awal Reagen terhadap Kadar Glukosa Darah Metode Enzimatik Yayuk Kustiningsih; Nastiti Megawati; Jasmadi Joko Kartiko; Leka Lutpiatina
Medical Laboratory Technology Journal Vol 3, No 1 (2017): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (875.733 KB) | DOI: 10.31964/mltj.v3i1.147

Abstract

Pre-analysis phase is the first step that determines the quality of the examination results, in this case, is the preparation of glucose work reagent. Glucose is one of the clinical examination clinical parameters that are often done in the laboratory. This glucose examination uses enzymatic methods. Enzyme activity is influenced by several factors, namely temperature, pH, substrate level, enzyme level, and inhibitor. Glucose examination performed at a temperature of 20-25oC or 37oC, but there are still laboratories in the field that is less attention to the initial temperature conditions of this reagent. This study aims to determine the effect of initial temperature variations of reagents on blood glucose levels of enzymatic methods. The research used Quasi Experimental research method with Posttest-Only Control Group Design design. The sample of this research used serum of a respondent who had no history of Diabetes Mellitus disease with 4 replication at each treatment. The results of the study with the initial temperature of 10oC, 13oC, 16oC, 19oC, 22oC and 25oC (control) respectively of 62.71 mg / dL; 65,74 mg / dL; 72.45 mg / dL; 76.91 mg / dL; 89.12 mg / dL and 97.19 mg / dL, the results of this study showed an increase in glucose levels in each treatment. Based on the results of linear regression analysis obtained a significance value of 0.000 with α = 0.05, which means Ho is rejected, so it can be concluded that there is an influence of initial temperature variation of reagent on blood glucose level. For the next researcher can do research about the effect of the initial temperature of reagent 22-38 oC on blood glucose enzymatic method. And the influence of incubation time on blood glucose level of enzymatic method.
Angka kuman udara ruang rawat inap anak dengan dan tanpa air conditioner (AC) di rumah sakit Raimunah Raimunah; Leka Lutpiatina; Jasmadi Joko Kartiko; Wahdah Norsiah
Jurnal Skala Kesehatan Vol 9 No 1 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.815 KB) | DOI: 10.31964/jsk.v9i1.152

Abstract

Air microorganisms can be found in outdoor or indoor air, ventilation is a very important place in the exchange of indoor air. Ventilation system is divided into two, namely natural ventilation and artificial ventilation in the form of Air Conditioner (AC). This study aims to determine the description of the number of germs in the inpatient wards of children using Air Conditioner (AC) and non (AC) at the Hospital in Martapura city area. This type of research is descriptive survey, the population is the inpatient room of children using Air Conditioner (AC) and non (AC). Sampling using purposive sampling technique, that is as much as 8 room. The examination method used is by TPC (Total Plate Count) method. The results showed that the number of airborne bacterial infections using the Air Conditioner (AC) was 406 CFU/m3. The total number of non-living inpatients (AC) is 443 CFU/m3. The standard maximum number of microorganisms (CFU / m3) of treatment room according to Kepmenkes No.1204/ Menkes/SK/X/2004 is 200-500 CFU / m3.
Efektivitas Air Perasan Jeruk Nipis (Citrus aurantifolia) dalam Menghambat Pertumbuhan Escherichia coli Ratih Dewi Dwiyanti; hana nailah; Ahmad Muhlisin; Leka Lutpiatina
Jurnal Skala Kesehatan Vol 9 No 2 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (924.454 KB) | DOI: 10.31964/jsk.v9i2.161

Abstract

Lime (Citrus aurantifolia) can be used for cough medicine, sputum laxative, influenza, and acne medication. Lime contains useful chemical compounds, one of which is essential oils and flavonoids which function as antibacterials and play a very important role in inhibiting bacterial growth. The purpose of this study was to determine the effectiveness of lime juice (Citrus aurantifolia) at a concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and 100% on the growth of Escherichia coli in vitro. This research is experimental with post test only control group design. The research sample was lime juice. Antibacterial power testing using well diffusion method. Antibacterial power parameters were determined by measuring the inhibitory zone (mm) formed around the growth of test bacteria on Muller Hinton Agar media. The results of the study showed that lime juice inhibition zones against the growth of Eschericia coli at concentrations of 40%, 50%, 60%, 70%, 80%, 90% and 100% were 7.25mm, 13.25mm, 14.25mm, 16mm, 17mm, 18.25mm, and 20.75mm. Based on linear regression test obtained significant value of 0.000 <α 0.05 so it can be concluded that there is an effect of lime juice on Eschericia coli growth in vitro. It is recommended that further research be carried out to test the inhibitory power of lime juice using other methods or against bacteria of other species.
Staphylococcus aureus and Methicillin resistant Staphylococcus aureus From The Diabetic Ulcer Leka Lutpiatina; Nisa Nur Agistni Eriana
Medical Laboratory Technology Journal Vol 4, No 1 (2018): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (836.382 KB) | DOI: 10.31964/mltj.v4i1.182

Abstract

Infections of diabetic ulcers may occur because patients with diabetes mellitus have a weak immune system and the presence of high blood sugar into a strategic place for bacterial growth one of them is Staphylococcus aureus. Increased resistance of Staphylococcus aureus to various antibiotics, causing problems for the treatment of this infection. As by Methicillin-resistant Staphylococcus aureus (MRSA) This study aims to assess the presence of Staphylococcus aureus and Methicillin-resistant Staphylococcus aureus (MRSA) at diabetic ulcer patient at Banjarmasin city hospital. This research uses descriptive observational research type. The study sample was patients with grade 2 and three diabetic ulcers with a total of 30 samples. The result of the research found 14 Staphylococcus aureus bacteria, Staphylococcus epidermidis nine, Staphylococcus saprophyticus two and 5 Gram-negative bacteria. Of the 14 samples of Staphylococcus aureus, there were eight samples of cefoxitin-resistant samples. The conclusion was 47% of Staphylococcus aureus in patients with a diabetic ulcer at Banjarmasin City Hospital. There was a 57% Methicillin-resistant Staphylococcus aureus in the isolated Staphylococcus aureus found. Need further research on the resistance of antibiotics oxacillin and cefoxitin to other Staphylococcus sp bacteria derived from patient diabetic ulcers.
Improved Mycology Media Formula of Corn and Cow’s Hair For Accelerate Superficial Mycosis Growth Ratih Dewi Dwiyanti; Leka Lutpiatina
Medical Laboratory Technology Journal Vol 7, No 2 (2021): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v0i0.391

Abstract

Potato Dextrose Agar is growth and identification media of fungi that cause superficial mycoses, including Trichophyton rubrum and Trichophyton mentagrophytes with 10–14 days growth period. Modification media is needed to accelerate the growth of the fungus. Zea mays saccharata has a high carbohydrate, and cow’s hair contains high keratin, which can be used as a nutrient to replace fungal growth media. The study aimed to obtain a modified media formula containing Zea mays saccharata as a substitute for dextrose and cow's hair as a source of keratin to accelerate the growth of Trichophyton rubrum and Trichophyton mentagrophytes fungi. The modified media formula is mixing of Zea mays saccharata 15% to 25%, and cow's hair 20mg to 35mg. Acceleration of growth time based of when found microconidia and macroconidia, diameter measurements, and observations of texture and colonies color of Trichophyton rubrum and Trichophyton mentagrophytes. The study results are Zea mays saccharata media with the addition of cow hair for Trichophyton rubrum grew 11 days with 10%+20mg formula and grew only seven days with 25%+35mg formula. Modified media for Trichophyton mentagrophytes grew seven days for 10%+20mg formula, while it was only three days with 25%+35mg formula. The media formula with the fastest growth rate for Trichophyton rubrum and Trichophyton mentagrophytes was at a concentration of 25%+35mg. The Advanced Modified Media can become a patent medium for faster growth of superficial fungi at a lower cost. Further research needs to be done on how long this media can be stored for long-term use.
Madu lebah kelulut (Trigona Spp.) dalam aktifitas terhadap bakteri Staphylococcus aureus resisten Muhammad Ma'ruf; Gina Alia Mawaddah; Nisa Nur Agistni Eriana; Farah Indah Swari; Syaidatul Aslamiyah; Leka Lutpiatina
Jurnal Skala Kesehatan Vol 9 No 1 (2018): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.153 KB) | DOI: 10.31964/jsk.v9i1.151

Abstract

Infection caused by Staphylococcus aureus bacteria becomes a very serious problem because of the increased resistance of these bacteria to various types of antibiotics. Honey has antibacterial activity because it contains water, acidity and inhibitor compounds namely flavonoids. Honey can be produced Trigona spp. This study aims to Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) and effectiveness of honey bee kelulut (Trigona spp.) Against Staphylococcus aureus resistant cephalosporin bacteria in vitro. This research is true experiment with postest only control group design. The results of the study were measured by MIC showed no clarity at concentrations of 60 mg / ml, 70 mg / ml, 80 mg / ml, 90 mg / ml and and clarity at concentrations of 100 mg / ml, and MBC at concentrations of 60 mg / ml of 151 colonies, 70 mg / ml of 105 colonies, 80 mg / ml of 55 colonies, 90 mg / ml of 16 colonies and 100 mg / ml of 0 colonies. The effective concentration in killing Staphylococcus aureus resistant cephalosporin is 100 mg / ml
EFEKTIVITAS EKTRAK PROPOLIS LEBAH KELULUT (Trigona spp) DALAM MENGHAMBAT PERTUMBUHAN Salmonella typhi, Staphylococcus aureus DAN Candida albicans Leka Lutpiatina
Jurnal Skala Kesehatan Vol 6 No 1 (2015): JURNAL SKALA KESEHATAN
Publisher : Politeknik Kementerian Kesehatan Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.921 KB) | DOI: 10.31964/jsk.v6i1.32

Abstract

Propolis mempunyai khasiat sebagai antikanker, antivirus, antifungi dan antibiotika (Haryanto et al., 2012). Penelitian Agustina (2007), dihasilkan ekstrak propolis lebah asal Malang dapat mempengaruhi dan menghambat pertumbuhan bakteri gram positif Staphylococcus epidermidis pada konsentrasi 60% dan bakteri gram negatif Pseudomonas aeruginosa pada konsentrasi 70%. Penelitian mengenai efektivitas antibakteri dan anti jamur propolis dan lebah Trigona spp asal provinsi Kalimantan Selatan sejauh pengetahuan penulis belum pernah dilakukan. Tujuan umum penelitian ini untuk mengetahui konsentrasi yang efektif dari ekstrak propolis  lebah kelulut (Trigona spp) dalam menghambat pertumbuhan bakteri Salmonella typhi Staphylococcus aureus dan Candida albicans. Hipotesis penelitian adalah Ekstrak propolis lebah kelulut (Trigona spp) efektif dalam menghambat pertumbuhan bakteri Salmonella typhi, Staphylococcus aureus dan Candida albicans. Jenis penelitian yang digunakan dalam penelitian ini adalah metode true eksperiment berupa rancangan-rancangan eksperimen sungguhan dengan menggunakan  rancangan Postest Only Control Group Design (Notoatmodjo, 2010). Bahan penelitian adalah propolis dari sarang lebah kelulut (Trigona spp) yang ada di daerah Banjarbaru, dengan kriteria sarang yang bertekstur rapuh dan berwarna gelap. Data yang diperoleh ditabulasikan dan dilakukan analisis secara statistik dengan one way anova dan Kruskal Wallis α 0,05 pada tingkat kepercayaan 95%. Hasil penelitian konsentrasi yang efektif dari ekstrak propolis  untuk Salmonella typhi dan Staphylococcus aureus adalah 100% dan  tidak terdapat zone hambat untuk Candida albicans. Saran perlu dilakukan uji aktivitas antimikroba propolis lebah kelulut (Trigona spp) asal daerah Kalimantan Selatan terhadap candida albicans dengan metode dilusi. Kata Kunci : Ekstrak Propolis, Trigona spp, Salmonella typhi, Staphylococcus aureus, Candida albicans