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UJI ADAPTASI MULTIMUSIM KARAKTER FENOTIP KULTIVAR MELODI GAMA 3 (CUCUMIS MELO L.): USAHA PENGUATAN INDUSTRI BENIH NASIONAL Sidiq, Yasir; Maryanto, Sigit Dwi; Daryono, Budi Setiadi
Prosiding Seminar Biologi Vol 10, No 2 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

Tanaman melon (Cucumis melo  L.) merupakan tanaman pertanian yang banyak dibudidayakan untuk konsumsi buahnya. Salah satu permasalahan dalam budidaya melon adalah benih yang diperoleh secara impor sehingga harganya relatif mahal. Perakitan kultivar MelodiGama 3 (MG3) merupakan strategi untuk mengurangi ketergantungan terhadap benih impor. Kultivar MG3 merupakan hasil persilangan antara kultivar ? MG1 dengan ? La-3 (tahun 2011). Tujuan penelitian ini untuk mengetahui kemampuan adaptasi kultivar MG3 terhadap kondisi lingkungan pada musim kemarau (tahun 2012) dan musim penghujan (tahun 2013) dan mengamati kestabilan kultivar MG3 di kedua musim tersebut di demplot milik Kebun Pendidikan Penelitian dan Pengembangan Pertanian (KP4) Universitas Gadjah Mada (UGM), Yogyakarta. Hasil yang diperoleh menunjukkan bahwa karakter melon MG3 dari penanaman musim penghujan umumnya lebih unggul daripada penanaman musim kemarau. Melon MG3 penanaman musim penghujan unggul pada karakter berat buah, keliling buah, ukuran buah, dan berat biji. Namun terdapat penurunan karakter unggul yaitu tingkat kemanisan (brix) dari 11,53% menjadi 9,98%. Hasil tersebut terjadi karena musim penghujan yang mempengaruhi kadar air dalam buah sehingga tingkat kemanisanya menurun. Kata kunci: MG3, Karakter Fenotip, Pemuliaan Tanaman
Studi Etnobotani Dioscorea spp. (Dioscoreaceae) dan Kearifan Budaya Lokal Masyarakat di Sekitar Hutan Wonosadi Gunung Kidul Yogyakarta Purnomo, Purnomo; Daryono, Budi Setiadi; Rugayah, Rugayah; Sumardi, Issirep
Jurnal Natur Indonesia Vol 14, No 3 (2012)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.947 KB) | DOI: 10.31258/jnat.14.3.191-198

Abstract

Dioscorea spp. were cultivated as alternative food in the garden and yard on dry season, and it’s planted by peoples in the villages. The objectives of the research are to identify the species, the tuber use, and the local wisdom to support the conservation of Dioscorea around Wonosadi forest. Samples and data collection was conducted in 15 villages around Wonosadi forest. Interview survey was done on peoples who are cultivate yam species (Dioscorea spp.) in their garden. Research were emphasized to tuber characteristic, traditional uses, and local wisdom to supporting yam conservation. Data was analyzed descriptively to illustrate the kind of local usage and local wisdom of connect with Dioscorea spp. The results showed that there were found species of gembili (D. esculenta (Lour.) Burk.), uwi (D. alata L.), and gadung (D. hispida dennst.) as cultivated plants, and tomboreso (D. pentaphylla L.), gembolo (D. bulbifera L.), and jebubuk (D. numularia Lamk.) as wild species in the forest. Tuber is raw material for many kind of food based on carbohydrate content. Gadung were used as fickle food with high diversification and insecticide for rice and corn. Peoples more consume gembili and uwi than gembolo and others species as rice substitute at dry season. The local wisdom were indicated that peoples still consume gembili and uwi as rice substitute, while tuber also used as sesaji in traditional concept jajan pasar or tukon pasar, and tuber of uwi was used as diet for diabetes complaint, and row material of uwi ungu tuber was used effectively for diarrhea complaint in the villages.
Karakter Fenotipik Tanaman Stroberi Festival (Fragaria x ananassa D.) Hasil Induksi Kolkisin Pada Konsentrasi 0,05% dan 0,01% Riza Aristya, Ganies; Setiadi Daryono, Budi
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Strawberries were a lot of holticulture commodities consumed by the people of Indonesia. Strawberry consumption continues to increase over time in Indonesia but not matched by production. This it because the strawberry is native to subtropical climate so that production in Indonesia limited in the plateau regional still affected by the tropical climate. Therefore, it is necessary to increase the production of both quality and quantity of strawberries, one with plant breeding through the induction of colchicine. The objective of this research were to determine the differences in phenotypic characters of strawberry plants with the control and induction results to determine the length of time and the induction colchicine 0,05% and 0,01% on the Festival cultivar of strawberry most effective. Research were done by inducing with 0,05% and 0,01% colchicine in leaves, roots 24 hours and 36 hour, the root and leaves of 24 and 36 hour. The observed for 6 months long leaf growth, leaf width,number of leaves, stem circumference, plant height, bloom vast flower and volumes of the fruit. The results indicate that the induction of the leaf growth at the optimal parameter leaf lenghth, leaf width and number of leaves. While 24-hour induction of root growth at optimum height and volume of fruitplants, then root induction at 36 hour to flowers bloom. Induction in root and leaves 36 hour optimum growth on trunck circumference. From this study it could be concluded that there are differences in the character of the strawberry crop cultivar Festival phenotype induction results colchicine 0,05% and 0,01% with control. Induction for 36 hours and induction on leaves was most effective for induction of strawberry cultivar Festival.Keywords: character phenotype, colchicine, festival
Aplikasi Teknologi Budidaya Melon (Cucumis melo L.) Kultivar Gama Melon Basket di Lahan Karst Pantai Porok Kabupaten Gunungkidul D.I.Yogyakarta Setiadi Daryono, Budi; Rizal Ibrohim, Asep; Dwi Maryanto, Sigit
Biogenesis Vol 3, No 1 (2015)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Melon (Cucumis melo L.) is one of favorite fruits for Indonesian. The fruit is sweet with good odor, and has a high nutrient content. Efforts to increase the production of melon can be reached by improving cultivation technology along with seeds that have better quality, such as resistance to pests and disease, and high production. The low production of melon in farmer fields is often caused by use of local varieties with less improvement technology in crop management. Faculty of Biology has developed a new melon cultivars called Gama Melon Basket. Test the stability of melon cultivars at various enviromental condition have been conducted, except for karst land. Therefore, the stability test of Gama Melon Basket phenotype character cultivated in karst land of Porok Beach of Gunungkidul Yogyakarta is required. The purpose of research was to study stability and uniformity of phenotypic character on Gama Melon Basket cultivar cultivated in karst land of Porok Beach, Gunungkidul, DIY and comparison of its phenotypic characters using modern, intermediate-modern, and traditional methods of cultivation. The research was carried out by cultivating Gama Melon Basket at Coastal Land of Porok Beach with modern, intermediate-modern and traditional cultivation methods. Every week phenotype characters of plants were observed until harvest. Results of quantitative characters of Gama Melon Basket showed that intermediate-modern cultivation method produced highest melon production compare to other methods.The result of Gama Melon Basket using intermediate-modern method is 1552 grams for average weight, fruit circumference is 46,19 cm, fruit diameter is 14,3 cm, fruit length is 14,08 cm, skin thickness is 0,6 cm and thick meat is 0,62 cm. The results revealed that intermediate-modern method is more advisable for melon cultivation in karst land of Porok Beach compared with other methods. Karst Land of Porok Beach has a potential for melon cultivation and production in Gunungkidul.Keywords: Gama Melon Basket, Gunungkidul, karst, phenotype, Porok beach
Development of Random Amplified Polymorphism DNA Markers Linked to Powdery Mildew Resistance Gene in Melon Daryono, Budi Setiadi; Aristya, Ganies Riza; Kasiamdari, Rina Sri
Indonesian Journal of Biotechnology Vol 16, No 2 (2011)
Publisher : Universitas Gadjah Mada

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Abstract

A random amplified polymorphic DNA (RAPD) marker linked to powdery mildew resistance gene (Pm-I) in melon PI 371795 was reported. However, the RAPD marker has problem in scoring. To detect powdery mildew resistance gene (Pm-I) in melon accurately, the RAPD marker was cloned and sequenced to design sequence characterized amplified region (SCAR) markers. SCAPMAR5 marker derived from pUBC411 primer yielded a single DNA band at 1061 bp. Segregation of SCAPMAR5 marker in bulk of F2 plants demonstrated that the marker was co-segregated with RAPD marker from which the SCAR marker was originated. Moreover, results of SCAR analysis in diverse melons showed SCAPMAR5 primers obtained a single 1061 bp linked to Pm-I in resistant melon PI 371795 and PMAR5. On the other hand, SCAPMAR5 failed to detect Pm-I in susceptible melons. Results of this study revealed that SCAR analysis not only confirmed melons that had been clearly scored for resistance to Pm-I evaluated by RAPD markers, but also clarified the ambiguous resistance results obtained by the RAPD markers.   Key words: Cucumis melo L., Pm-I, RAPD, SCAPMAR5
Application of Multiplex RT-PCR for Detection of Cucurbit-infecting Tobamovirus Daryono, Budi Setiadi; Natsuaki, Keiko T.
Indonesian Journal of Biotechnology Vol 16, No 1 (2011)
Publisher : Universitas Gadjah Mada

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Abstract

Cucumber green mottle mosaic virus (CGMMV) and Kyuri green mottle mosaic virus (KGMMV) are seed borne viruses and they are also transmitted mechanically during agricultural practice and through water. Hence, these viruses have potential diseases widely distributed throughout the world. To detect different strains of CGMMV and KGMMV, several specific primers for each virus were designed for single and multiplex RT-PCR. The results of single and multiplex RT-PCR showed that CGMMV was detected in zucchini isolated in Bali-Indonesia, while KGMMV was detected both in zucchini isolated in Bali-Indonesia and Cucumis metuliferus isolated in Thailand. Furthermore, artificial co-infection of these two viruses was prepared and carried out using two different ways of viral RNAs extraction. Based on the results, it could be reported that viral RNAs for cDNA amplification by multiplex RT-PCR could be extracted from a mixture of infected leaves or separate extraction of each viruses infected leaves. In addition, results presented in this study demonstrated the application of multiplex RT-PCR to simultaneously detect CGMMV and KGMMV from cucurbit leaves using a mixture of four primers and its feasibility as a sensitive and rapid laboratory assay. Since, no multiplex RT-PCR technique has been described for the detection of CGMMV and KGMMV, this technique can be a good option for sensitive and reliable tool for detection of two major cucurbit infecting Tobamoviruses.Keywords : Cucurbit infecting Tobamovirus, multiplex RT-PCR, seed borne viruses
Genetic Variation Analysis of Mold (Magnaporthe oryzae B.Couch) Using Random Amplified Polymorphic DNA Pramono, Ajeng Kusumaningtyas; Daryono, Budi Setiadi
Indonesian Journal of Biotechnology Vol 17, No 2 (2012)
Publisher : Universitas Gadjah Mada

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Abstract

Magnaporthe oryzae B.Couch is a host-specific fungi, certain strain only infect certain host plant species. Genetic variety among M. oryzae isolates was explained by dendogram which was constructed using similarity data of Random Amplified Polymorphic DNA (RAPD). Dendogram construction was achieved by computer software, Numerical Taxonomy System (NTSYS). The aim of the research were to study the genetic variation among M. Oryzae using RAPD and to construct a dendogram of genetic similarities among the ten isolates from green foxtail (Setaria viridis L.), finger millet (Eleusine coracana L.) and rice (Oryza sativa L.).RAPD was performed in 30 cycles using 5 primers (OPA-02, OPA-03, OPA-04, OPA-05, OPA-07). Polymorphism data was used to constructed dendogram using Dice index and Unweighted Pair Group Method with Arithmetic Mean (UPGMA) in NTSYS software. There were 68 polymorphism fragments from 74 amplified fragments.Three clusters were formed in the dendrogram, based on host pathotype: foxtail millet type, finger millet type and rice type. There were two subclusters in foxtail millet type based on mating type, MAT1-1 dan MAT1-2. Thus, RAPD could be used as a method for genetic variation analysis of Magnaporthe oryzae to show host-specific specificity.Key words: Magnaporthe oryzae, RAPD, mating type
PENGEMBANGAN SENTRA BUDIDAYA MELON DI PANTAI BOCOR KABUPATEN KEBUMEN MELALUI IMPLEMENTASI EDUCATION FOR SUSTAINABLE DEVELOPMENT Daryono, Budi Setiadi; Purnomo, Purnomo; Sidiq, Yasir; Maryanto, Sigit Dwi
Bioeksperimen: Jurnal Penelitian Biologi Vol 2, No 1: Maret 2016
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Pesisir Pantai Bocor yang terletak di selatan Kabupaten Kebumen, merupakan salah satu lokasi yang berpotensi sebagai lahan pertanian. Sepanjang Pantai Bocor terbentang gumuk pasir yang berfungsi sebagai penghalang angin dan gelombang. Wilayah tersebut dimanfaatkan sebagai lokasi menanam melon yang menjadi komoditas unggulan daerah. Oleh sebab itu dikembangkan melon ramah lingkungan untuk menjaga kelestarian gumuk pasir. Upaya pengembangan melon ramah lingkungan termasuk dalam Education for Sustainable Development (ESD).  yang didanai UGM. Pogram ini bertujuan untuk pengembangan masyarakat Desa Setrojenar, Kabupaten Kebumen berbasis ESD. Kegiatan program ini antara lain sosialisasi tentang pelestarian gumuk pasir, pengenalan alternatif tanaman pangan yaitu umbi-umbian, penanaman tanaman Casuarina equisetifolia Linn. sebagai barier, dan pendampingan budidaya melon menggunakan potensi lokal oleh petani. Hasil kegiatan antara lain partisipasi masyarakat tinggi, gumuk pasir tidak rusak, tanaman barier dapat tumbuh baik dan petani dapat panen melon dengan kualitas baik. Kegiatan ini diharapkan dapat menjadi role model bagi masyarakat untuk terus mengembangkan budidaya melon dan tetap menjaga kelestarian gumuk pasir.
Callus Induction and Differentiation on Melon From In Vitro Culture with The Addition of Indole Acetic Acid and Benzyl Amino Purine Growth Regulator Probowati, Wiwit; Daryono, Budi Setiadi
PLANTA TROPIKA: Jurnal Agrosains (Journal of Agro Science) Vol 6, No 1 (2018)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.2018.076.15-21

Abstract

Melon plants (Cucumis melo L.) are susceptible to disease attacks primarily caused by viruses. One of the viruses often attacking melon plants is Cucumber mosaic virus (CMV). Previous studies have shown that melon callus produced from in vitro culture is detected to have CMV resistance gene (Creb-2). However, the trait of resistance to CMV in that melon (F2) is still segregated. To know the stability of the Creb-2 gene, further research is needed to detect the gene in the differentiated callus. This study aims to  callus induce and callus differentiate melon. Firstly, melon seeds were grown on ¼ MS medium with addition of 2 mg / L 2.4-Dichlorophenoxyacetate and 1 mg / L BAP (Benzyl Amino Purine) to grow callus. Secondly, the callus was differentiated in MS medium with the addition of 2,4-D , BAP and IAA in different doses. 0 mg / L; 0.01 mg / L 2.4-D and 0.1 mg / L BAP, and MS medium with 0.01 mg / L; 0.1 mg / L; 1 mg / L IAA (Iodole Acetic Acid) and 0.1 mg / L BAP. The results showed that callus was successfully differentiated into root and it grew optimally in MS medium with 0.01 mg / L 2.4-D: 0.1 mg / L BAP and 1 mg / L IAA: 0.1 mg / L BAP . Therefore the roots can be used as isolation samples and DNA amplification to detect stability of the Creb-2 gene.
PENGEMBANGAN SENTRA BUDIDAYA MELON DI PANTAI BOCOR KABUPATEN KEBUMEN MELALUI IMPLEMENTASI EDUCATION FOR SUSTAINABLE DEVELOPMENT Daryono, Budi Setiadi; Purnomo, Purnomo; Sidiq, Yasir; Maryanto, Sigit Dwi
Bioeksperimen: Jurnal Penelitian Biologi Vol 2, No 1: Maret 2016
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v2i1.1580

Abstract

Pesisir Pantai Bocor yang terletak di selatan Kabupaten Kebumen, merupakan salah satu lokasi yang berpotensi sebagai lahan pertanian. Sepanjang Pantai Bocor terbentang gumuk pasir yang berfungsi sebagai penghalang angin dan gelombang. Wilayah tersebut dimanfaatkan sebagai lokasi menanam melon yang menjadi komoditas unggulan daerah. Oleh sebab itu dikembangkan melon ramah lingkungan untuk menjaga kelestarian gumuk pasir. Upaya pengembangan melon ramah lingkungan termasuk dalam Education for Sustainable Development (ESD).  yang didanai UGM. Pogram ini bertujuan untuk pengembangan masyarakat Desa Setrojenar, Kabupaten Kebumen berbasis ESD. Kegiatan program ini antara lain sosialisasi tentang pelestarian gumuk pasir, pengenalan alternatif tanaman pangan yaitu umbi-umbian, penanaman tanaman Casuarina equisetifolia Linn. sebagai barier, dan pendampingan budidaya melon menggunakan potensi lokal oleh petani. Hasil kegiatan antara lain partisipasi masyarakat tinggi, gumuk pasir tidak rusak, tanaman barier dapat tumbuh baik dan petani dapat panen melon dengan kualitas baik. Kegiatan ini diharapkan dapat menjadi role model bagi masyarakat untuk terus mengembangkan budidaya melon dan tetap menjaga kelestarian gumuk pasir.