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BUDI SETIADI DARYONO
Department Of Tropical Biology, Faculty Of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
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Journal : Biogenesis

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Analisis Variasi Genetik Melon (Cucumis melo L.) Kultivar Gama Melon Basket Dengan Metode Random Amplified Polymorphic DNA Nur Huda, Ikhsan; Setiadi Daryono, Budi
Biogenesis Vol 1, No 1 (2013)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Melon (Cucumis melo L.) cultivars Gama Melon Basket (GMB) is the result of plant breeding that has the potential to be a product of local melon seed in Indonesia. This cultivar phenotype characters was known, but its genetic variation is not further studied. This study aimed to analyze the genetic variation of melon cultivars GMB and compared with 13 commercial melons and four members of the family Cucurbitaceae such as; cucumber, cucumber queen, barteh, and cantaloupe (blewah). RAPD-PCR method with 11 random primer was used to evaluate genetic variation in melon. Level of genetic similarity was determined by the formula simple matching coefficient (SSM) and the dendogram constructed by clustering analysis unweighted pair-group using arithmeticaverages (UPGMA). Results of RAPD-PCR with 11 primer produced 165 loci and it contains 84.85 % polymorphic DNA and 15.15 % monomorphic DNA. Results of genetic variation analysis suggests that 15 commercial melons differ from groups of cucumber, cucumber queen, barteh and cantaloupe(blewah). Cultivars GMB has genetic marker that can be distinguished from other commercial melons. GMB cultivar have specific polymorphic DNA on the size of 285 bp generated by PMAR primer.Keywords: Cucumis melo L., Gama Melon Basket, genetic variation, RAPD
Deteksi Odontoglossum ringspot virus Terhadap Anggrek Alam di Hutan Wonosadi, Gunung Kidul K, Mahfut; Setiadi Daryono, Budi
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Wonosadi forest is managed forests by indigenous forest communities and is located in the hamlet of Duren and Sidorejo, Beji village, subdistrict Ngawen, Gunung Kidul regency, Yogyakarta. One of the biological richness contained in Wonosadi forest is very valuable natural orchids and need to be preserved. Management to the preservation of nature in the woods Wonosadi orchids can be done through natural orchid protection against infectious pathogens, including viruses. Odontoglossum ringspot virus (ORSV) is one type of orchid viruses infect most widely reported and has spread widely in the world, including in Indonesia. ORSV can cause severe damage to the orchid. Therefore, it is necessary to study to detect ORSV that infect wild orchids in the forest Wonosadi molecular basis for detecting the presence and pattern of infection ORSV as well as possible to reduce the severity of disease caused by infection with the virus. The purpose of this study is to assess the natural orchid ORSV infection in molecular Wonosadi forest that can be used as a database in an effort to protect against natural orchid Wonosadi forest. In the long term, the results of this study are very useful to support conservation efforts and determine appropriate conservation efforts for wild orchids in the forest Wonosadi. The initial phase of the study conducted a survey and collection of samples of wild orchids in the forest leaves that show symptoms of infection Wonosadi ORSV. Furthermore, molecular analysis performed with gene amplification Coat Protein ORSV using Reverse engineering Trancriptase Polymerase Chain Reaction (RT-PCR).Keywords: orchid soil, ORSV, RT-PCR, Wonosadi forest
Karakter Fenotipik Tanaman Stroberi Festival (Fragaria x ananassa D.) Hasil Induksi Kolkisin Pada Konsentrasi 0,05% dan 0,01% Riza Aristya, Ganies; Setiadi Daryono, Budi
Biogenesis Vol 2, No 2 (2014)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Strawberries were a lot of holticulture commodities consumed by the people of Indonesia. Strawberry consumption continues to increase over time in Indonesia but not matched by production. This it because the strawberry is native to subtropical climate so that production in Indonesia limited in the plateau regional still affected by the tropical climate. Therefore, it is necessary to increase the production of both quality and quantity of strawberries, one with plant breeding through the induction of colchicine. The objective of this research were to determine the differences in phenotypic characters of strawberry plants with the control and induction results to determine the length of time and the induction colchicine 0,05% and 0,01% on the Festival cultivar of strawberry most effective. Research were done by inducing with 0,05% and 0,01% colchicine in leaves, roots 24 hours and 36 hour, the root and leaves of 24 and 36 hour. The observed for 6 months long leaf growth, leaf width,number of leaves, stem circumference, plant height, bloom vast flower and volumes of the fruit. The results indicate that the induction of the leaf growth at the optimal parameter leaf lenghth, leaf width and number of leaves. While 24-hour induction of root growth at optimum height and volume of fruitplants, then root induction at 36 hour to flowers bloom. Induction in root and leaves 36 hour optimum growth on trunck circumference. From this study it could be concluded that there are differences in the character of the strawberry crop cultivar Festival phenotype induction results colchicine 0,05% and 0,01% with control. Induction for 36 hours and induction on leaves was most effective for induction of strawberry cultivar Festival.Keywords: character phenotype, colchicine, festival
Aplikasi Teknologi Budidaya Melon (Cucumis melo L.) Kultivar Gama Melon Basket di Lahan Karst Pantai Porok Kabupaten Gunungkidul D.I.Yogyakarta Setiadi Daryono, Budi; Rizal Ibrohim, Asep; Dwi Maryanto, Sigit
Biogenesis Vol 3, No 1 (2015)
Publisher : Jurusan Biologi UIN Alauddin Makassar

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Abstract

Melon (Cucumis melo L.) is one of favorite fruits for Indonesian. The fruit is sweet with good odor, and has a high nutrient content. Efforts to increase the production of melon can be reached by improving cultivation technology along with seeds that have better quality, such as resistance to pests and disease, and high production. The low production of melon in farmer fields is often caused by use of local varieties with less improvement technology in crop management. Faculty of Biology has developed a new melon cultivars called Gama Melon Basket. Test the stability of melon cultivars at various enviromental condition have been conducted, except for karst land. Therefore, the stability test of Gama Melon Basket phenotype character cultivated in karst land of Porok Beach of Gunungkidul Yogyakarta is required. The purpose of research was to study stability and uniformity of phenotypic character on Gama Melon Basket cultivar cultivated in karst land of Porok Beach, Gunungkidul, DIY and comparison of its phenotypic characters using modern, intermediate-modern, and traditional methods of cultivation. The research was carried out by cultivating Gama Melon Basket at Coastal Land of Porok Beach with modern, intermediate-modern and traditional cultivation methods. Every week phenotype characters of plants were observed until harvest. Results of quantitative characters of Gama Melon Basket showed that intermediate-modern cultivation method produced highest melon production compare to other methods.The result of Gama Melon Basket using intermediate-modern method is 1552 grams for average weight, fruit circumference is 46,19 cm, fruit diameter is 14,3 cm, fruit length is 14,08 cm, skin thickness is 0,6 cm and thick meat is 0,62 cm. The results revealed that intermediate-modern method is more advisable for melon cultivation in karst land of Porok Beach compared with other methods. Karst Land of Porok Beach has a potential for melon cultivation and production in Gunungkidul.Keywords: Gama Melon Basket, Gunungkidul, karst, phenotype, Porok beach
Analisis Variasi Genetik Melon (Cucumis melo L.) Kultivar Gama Melon Basket Dengan Metode Random Amplified Polymorphic DNA Ikhsan Nur Huda; Budi Setiadi Daryono
Biogenesis: Jurnal Ilmiah Biologi Vol 1 No 1 (2013)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v1i1.446

Abstract

Melon (Cucumis melo L.) cultivars Gama Melon Basket (GMB) is the result of plant breeding that has the potential to be a product of local melon seed in Indonesia. This cultivar phenotype characters was known, but its genetic variation is not further studied. This study aimed to analyze the genetic variation of melon cultivars GMB and compared with 13 commercial melons and four members of the family Cucurbitaceae such as; cucumber, cucumber queen, barteh, and cantaloupe (blewah). RAPD-PCR method with 11 random primer was used to evaluate genetic variation in melon. Level of genetic similarity was determined by the formula simple matching coefficient (SSM) and the dendogram constructed by clustering analysis unweighted pair-group using arithmeticaverages (UPGMA). Results of RAPD-PCR with 11 primer produced 165 loci and it contains 84.85 % polymorphic DNA and 15.15 % monomorphic DNA. Results of genetic variation analysis suggests that 15 commercial melons differ from groups of cucumber, cucumber queen, barteh and cantaloupe(blewah). Cultivars GMB has genetic marker that can be distinguished from other commercial melons. GMB cultivar have specific polymorphic DNA on the size of 285 bp generated by PMAR primer.Keywords: Cucumis melo L., Gama Melon Basket, genetic variation, RAPD
Karakter Fenotipik Tanaman Stroberi Festival (Fragaria x ananassa D.) Hasil Induksi Kolkisin Pada Konsentrasi 0,05% dan 0,01% Ganies Riza Aristya; Budi Setiadi Daryono
Biogenesis: Jurnal Ilmiah Biologi Vol 2 No 2 (2014)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v2i2.470

Abstract

Strawberries were a lot of holticulture commodities consumed by the people of Indonesia. Strawberry consumption continues to increase over time in Indonesia but not matched by production. This it because the strawberry is native to subtropical climate so that production in Indonesia limited in the plateau regional still affected by the tropical climate. Therefore, it is necessary to increase the production of both quality and quantity of strawberries, one with plant breeding through the induction of colchicine. The objective of this research were to determine the differences in phenotypic characters of strawberry plants with the control and induction results to determine the length of time and the induction colchicine 0,05% and 0,01% on the Festival cultivar of strawberry most effective. Research were done by inducing with 0,05% and 0,01% colchicine in leaves, roots 24 hours and 36 hour, the root and leaves of 24 and 36 hour. The observed for 6 months long leaf growth, leaf width,number of leaves, stem circumference, plant height, bloom vast flower and volumes of the fruit. The results indicate that the induction of the leaf growth at the optimal parameter leaf lenghth, leaf width and number of leaves. While 24-hour induction of root growth at optimum height and volume of fruitplants, then root induction at 36 hour to flowers bloom. Induction in root and leaves 36 hour optimum growth on trunck circumference. From this study it could be concluded that there are differences in the character of the strawberry crop cultivar Festival phenotype induction results colchicine 0,05% and 0,01% with control. Induction for 36 hours and induction on leaves was most effective for induction of strawberry cultivar Festival.Keywords: character phenotype, colchicine, festival
Deteksi Odontoglossum ringspot virus Terhadap Anggrek Alam di Hutan Wonosadi, Gunung Kidul Mahfut K; Budi Setiadi Daryono
Biogenesis: Jurnal Ilmiah Biologi Vol 2 No 2 (2014)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v2i2.475

Abstract

Wonosadi forest is managed forests by indigenous forest communities and is located in the hamlet of Duren and Sidorejo, Beji village, subdistrict Ngawen, Gunung Kidul regency, Yogyakarta. One of the biological richness contained in Wonosadi forest is very valuable natural orchids and need to be preserved. Management to the preservation of nature in the woods Wonosadi orchids can be done through natural orchid protection against infectious pathogens, including viruses. Odontoglossum ringspot virus (ORSV) is one type of orchid viruses infect most widely reported and has spread widely in the world, including in Indonesia. ORSV can cause severe damage to the orchid. Therefore, it is necessary to study to detect ORSV that infect wild orchids in the forest Wonosadi molecular basis for detecting the presence and pattern of infection ORSV as well as possible to reduce the severity of disease caused by infection with the virus. The purpose of this study is to assess the natural orchid ORSV infection in molecular Wonosadi forest that can be used as a database in an effort to protect against natural orchid Wonosadi forest. In the long term, the results of this study are very useful to support conservation efforts and determine appropriate conservation efforts for wild orchids in the forest Wonosadi. The initial phase of the study conducted a survey and collection of samples of wild orchids in the forest leaves that show symptoms of infection Wonosadi ORSV. Furthermore, molecular analysis performed with gene amplification Coat Protein ORSV using Reverse engineering Trancriptase Polymerase Chain Reaction (RT-PCR).Keywords: orchid soil, ORSV, RT-PCR, Wonosadi forest
Aplikasi Teknologi Budidaya Melon (Cucumis melo L.) Kultivar Gama Melon Basket di Lahan Karst Pantai Porok Kabupaten Gunungkidul D.I.Yogyakarta Budi Setiadi Daryono; Asep Rizal Ibrohim; Sigit Dwi Maryanto
Biogenesis: Jurnal Ilmiah Biologi Vol 3 No 1 (2015)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v3i1.565

Abstract

Melon (Cucumis melo L.) is one of favorite fruits for Indonesian. The fruit is sweet with good odor, and has a high nutrient content. Efforts to increase the production of melon can be reached by improving cultivation technology along with seeds that have better quality, such as resistance to pests and disease, and high production. The low production of melon in farmer fields is often caused by use of local varieties with less improvement technology in crop management. Faculty of Biology has developed a new melon cultivars called Gama Melon Basket. Test the stability of melon cultivars at various enviromental condition have been conducted, except for karst land. Therefore, the stability test of Gama Melon Basket phenotype character cultivated in karst land of Porok Beach of Gunungkidul Yogyakarta is required. The purpose of research was to study stability and uniformity of phenotypic character on Gama Melon Basket cultivar cultivated in karst land of Porok Beach, Gunungkidul, DIY and comparison of its phenotypic characters using modern, intermediate-modern, and traditional methods of cultivation. The research was carried out by cultivating Gama Melon Basket at Coastal Land of Porok Beach with modern, intermediate-modern and traditional cultivation methods. Every week phenotype characters of plants were observed until harvest. Results of quantitative characters of Gama Melon Basket showed that intermediate-modern cultivation method produced highest melon production compare to other methods.The result of Gama Melon Basket using intermediate-modern method is 1552 grams for average weight, fruit circumference is 46,19 cm, fruit diameter is 14,3 cm, fruit length is 14,08 cm, skin thickness is 0,6 cm and thick meat is 0,62 cm. The results revealed that intermediate-modern method is more advisable for melon cultivation in karst land of Porok Beach compared with other methods. Karst Land of Porok Beach has a potential for melon cultivation and production in Gunungkidul.Keywords: Gama Melon Basket, Gunungkidul, karst, phenotype, Porok beach
Derajat Ploidi Jahe Merah (Zingiber officinale Roxb. var. rubrum Rosc.) Hasil Induksi Dengan Kolkisin Meiliana Friska; Budi Setiadi Daryono
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3433

Abstract

Red ginger (Zingiber officinale Roxb. var. rubrum Rosc.) is one of the herb plants that often being used as a spice, traditional medicines, sweets, drinks, and material export commodities. The ginger’s rhizome is the most common because it is good for health freshness, and spice for food. The spicy from the ginger comes from ketone compounds ‘gingerol’. This research aims to produce polyploidy crops in red ginger by observing the changes of phenotype characters on the leaves, stems and rhizome resulted from colchicine induction. This research was based on experimental method with Randomized design with two factors, which were colchicine concentration (0,05%, 0,1% and 0,2%) and incubation time (6, 12 and 24 hours). Ploidy level was observed using flow cytometry technique. The data were analyzed using Analysis of Variance (ANOVA) and statistical data processing was done using F test at α = 5%. If the result shows any significant differences then was followed with Duncan Multiple Range Test (DMRT). The results showed that the change in the ploidy degree of red ginger was seen in K1W2 (0,05 % concentration of incubation time 12 hours) with increasing in ploidy from the diploid (2n) to mixoploid (2n= 2x+4x).
Uji Ketahanan Tujuh Kultivar Jagung (Zea mays L.) Terhadap Penyakit Bulai (Peronosclerospora spp.) Budi Setiadi Daryono; Anisa Parazulfa; Purnomo Purnomo
Biogenesis: Jurnal Ilmiah Biologi Vol 6 No 1 (2018)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v6i1.4175

Abstract

Maize (Zea mays L.) is one of important food in Indonesia. Java Downy Mildew is a plant disease caused by Peronosclerospora spp. This specific fungus attacks corn plants, especially the leaves. The purpose of this study was to examine maize cultivars resistance to Java Downy Mildew.  This research was conducted at the land owned by Agrotechnology Innovation Center (PIAT) UGM located in Kalitirto Village, Berbah Subdistrict, Sleman Regency, Special Region of Yogyakarta. The main ingredients used in this study included maize with 7 different cultivars: Gama GS, Gama SG, Bisi 816, Pioneer 21, Lagaligo, Talenta, and Pulut. This research was conducted using Randomized Block Design with 7 treatments and 2 replications. The results showed that Talenta, Lagaligo and Bisi-816 were resistant to downy mildew. Pioneer-21 quite resistant to downy mildew. Cultivars Gama GS, Gama SG and Pulut were not resistant to downy mildew. This study provides new information related to the infection of downy mildew especially in Yogyakarta.
Co-Authors ., Sudarsono Abiyyayumna Rif’at Chasnaurosyiqoh Abrory Agus Cahya Pramana Achmad Amzeri AGUS HERY SUSANTO Agus Nuryanto Agus Setiawan Ajeng Kusumaningtyas Pramono Ajeng Kusumaningtyas Pramono, Ajeng Kusumaningtyas Al-Mughni, Eka Wasi? Alin Liana Anahtadiya Nurfa Shochicha Anak Agung Gede Agung Andra Jausa Salsabila Angga Bintang Narzassi Anisa Parazulfa Annas, Muhammad Sabri Aprilia Sufi Subiastuti Arfa, Namira Nur Ari Indrianto Asep Rizal Ibrohim Asep Rizal Ibrohim, Asep Avia Purnama Saputri Ayyu Rahayu Bernardinus Pratama Budy Wiryawan Catur Ahda Darojatun Charisma Asri Fitrananda Darmastuti Deris Trian Rahmandhias Desi Oktaviani Diah Rachmawati Diah Rachmawati DIAN ARUNI KUMALAWATI Didi Usmadi Didik Indradewa Dinar Nugroho Pratomo E. Suharyanto, E. Eka Wasi’ Al-Mughni Endah Retnaningrum Endang Semiarti Estiyani Indraningsih Esty Nidianti Fadilah Husnun Faridatul Hidzroh Feren Putri Sholiha Fitri Nadifah Fitri Nadifah Galuh Tresnani Ganies Riza Ariestya Ganies Riza Aristya Gebby Agnessya Esa Oktavia Hani Christin Yambise Hendry T.S.S.G. Saragih Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih Hendry Tri Sakti Saragih Hermawan, Vera Hetty Nopianasanti Hindarsah, Ida I Dewa Putu Darma I Dewa Putu Darma I Dewa Putu Darma I Dewa Putu Dharma I Made Yudana I Putu Agus Hendra Wibawa I Wayan Suardana I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika I Wayan Swarautama Mahardhika IKA MUSTIKA Ikhsan Nur Huda Ikhsan Nur Huda Irma Nofitahesti Ishak, Muhammad Alif Ismail Ismail Isna Mustafiatul Ummah Issirep Sumardi Iwan Gunawan Iwan Roosdianto Iwan Roosdianto Iwan Satibi Joko Prastowo Joseph Chohansandhika Keiko T. Natsuaki Keiko T. Natsuaki, Keiko T. KENJI WAKUI Linda Oktavianingsih M.Pd. ., PROF. DR. I MADE YUDANA, M.Pd. M.Pd. ., PROF.DR.A.A. GEDE AGUNG, M.Pd. Mahfut Maryani Maryani Maryani Maryani Muhammad Bima Atmaja Muhammad Dylan Lawrie Nata Dwi Annisa Nizma Nia Fararid Askar Nugroho Nofriarno Nurachmad Bagas Indriarto Ovami, Debbi Chyntia Paristiowati, Maria Polikarpia Wilhelmina Bani Prabawani, Ratri Lila Probowati, Wiwit Puji Wulandari Purnomo - Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Purnomo Puti Hana Ramadhani Putu Ayu Damayanti Rahmadani, Wenny Deisshinta Rahmadilla Salsabila Mahdison Rajif Iryadi Rajif Iryadi Ratri Lila Prabawani REFLINUR REFLINUR Restiyanti Restiyanti Ridesti Rindyastuti Rina Sri Kasiamdari Rini Etika Ranis Rizko Hadi Rugayah Rugayah Sahni Damerianta Salma Mutiara Tsani Satria Wahyuni Seonarwan Hery Poerwanto Shita Tiara Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto Sigit Dwi Maryanto, Sigit Slamet Widiyanto Soenghoe Jang Sonjaya, Rasman Sri Wahyuningsih Sudrajat, Rochmat Tri Sukarti Moeljopawiro Sumarmi Sumarmi Supriyadi Supriyadi Susamto Somowiyarjo Susilawati Susilawati Sutomo Sutomo Sutomo Sutomo Tara Puri Ducha Rahmani Taufik Hidayatullah Tety Hartatik Teuku Nanda Saifullah Umar Hafidz Asy’ari Hasbullah, Umar Hafidz Asy’ari Utin Elsya Puspita Utin Elsya Puspita Wayan Tunas Artama Wayan Tunas Artama Wenny Deisshinta Rahmadani, Wenny Deisshinta Widya Asmara Wiko Arif Wibowo Wiko Arif Wibowo Wisnu Nurcahyo Yasir Sidiq Yuanita Rachmawati Yuanita Rachmawati, Yuanita Yuliara, I Made