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Akhmad Muhlisin
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Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Nursing Public Health

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MRSA dan VRSA pada Paramedis RSUD Ratu Zalecha Martapura Ratih Dewi Dwiyanti; Akhmad Muhlisin; Akhmad Muntaha
Medical Laboratory Technology Journal Vol. 1 No. 1 (2015): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (754.675 KB) | DOI: 10.31964/mltj.v1i1.5

Abstract

Abstact: Staphylococcus aureus strains isolated from hospital generally been resistant to many antimicrobial-resistant strains of Staphylococcus aureus to vancomycin was rarely reported. Strains of Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial infections that are multiresistant to antibiotics. This study aims to determine the presence of bacteria Methicillin-resistant Staphylococcus aureus (MRSA) and Vancomycin resistant Staphylococcus aureus (VRSA) on paramedics in the treatment room and the surgical ICU care hospitals Zalecha Martapura queen. This study used a descriptive survey method. The population in this study were nurses who worked in the surgical and ICU care that numbered 38 people. Sampling technique accidental sampling with a sample of 27 people. The survey results revealed 27 positive samples of Staphylococcus aureus 14 people, there is a sensitivity test results of 7 samples were resistant to cefoxitin (MRSA 26%), 4 samples were resistant to oxacilin and 7 samples were resistant to vancomycin (VRSA 26%). Concluded there is MRSA and VRSA in hospital Queen zalecha Martapura, it is advisable to increase the cleanliness of individuals and the use of personal protective equipment to reduce the incidence of nosocomial infections in the scope of the hospital.
Akurasi Hitung Jumlah Eritrosit Metode Manual dan Metode Otomatis Neni Oktiyani; fahriyan fahriyan; Akhmad Muhlisin
Medical Laboratory Technology Journal Vol. 3 No. 2 (2017): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (900.051 KB) | DOI: 10.31964/mltj.v3i2.166

Abstract

In a laboratory, an erythrocyte is done by using hemocytometer and microscope. The task is to measure and assess the size and shape of the erythrocyte. But this procedure is time consuming, complex and tedious. As a solution to this problem, to provide an automated, cost-effective and efficient alternative to detection and counting of erythrocyte, hematology analyzers are used. However, false results related either to erythrocyte or other parameters from complete blood count may be observed in several instances. The objective study was to compare the accuracy of erythrocyte count results of automatic hematological analysis by the manual method. The study is an analytic survey with a cross-sectional design. Erythrocyte counting is done by using three types of control blood, namely high, normal and low, with 9 repetitions. In high control blood, the mean erythrocytes count by the manual method was 7.08 million/μl with a bias value of 1.4%, while that by the automated method was 7.03 million/μl with a bias value of 0.7%. The mean erythrocyte count in normal control blood by the manual method was 4.50 million/μl with a bias value of 0.9%, while that by the automated method was 4.4 million/μl with a bias value of 2.4%. And in low control blood, the mean erythrocyte count by the manual method was 1.72 million/μl with 4.4% bias value, while that the automated method was 1.67 million/μl with 1.2% bias value. Statistical analysis showed no significant difference in accuracy between the erythrocyte count of manual methods with automated methods.
Co-Authors akhmad muntaha fahriyan fahriyan neni oktiyani Ratih Dewi Dwiyanti