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Paisal Paisal
Balai Litbang P2B2 Tanah Bumbu
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Nursing Public Health Veterinary

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Kesesuaian Hasil Pemeriksaan RT PCR, RDT NS1, dan RDT IgM Pasien Penyakit Dengue Paisal Paisal; Mukhlis Zuardi; Reni Herman
Medical Laboratory Technology Journal Vol. 2 No. 2 (2016): December
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (700.181 KB) | DOI: 10.31964/mltj.v2i2.97

Abstract

The incidence of dengue disease in the world is estimated at 390 million cases per year. In Indonesia, during 2013 there were 35-40 cases per 100.000 population, with a mortality rate of 0.73%. This study aimed to determine the suitability and the percentage of RT-PCR, RDT NS1, and RDT IgM detection examination. Samples were obtained from hospitals in Aceh province during 2012. The research samples reached 100 collected samples, it was only 82 samples that fulfill the analysis criteria. Cohen’s Kappa test result showed there was moderate suitability between RT-PCR and RDT NS1 (K=0,404, p = 0,000), and weak suitability between RT-PCR began RDT IgM (K=0,139, p = 0,046). While the percentage of detection for RT-PCR, RDT NS1, dan RDT IgM were 16%, 10%, and 60%. RDT IgM is the best alternative for laboratory examination in the hospital.
KONFIRMASI KEBERADAAN Fasciola gigantica DAN HOSPES PERANTARA DI LINGKUNGAN PEMUKIMAN EKOSISTEM RAWA KABUPATEN HULU SUNGAI UTARA, KALIMANTAN SELATAN Budi Hairani; Syarif Hidayat; Paisal Paisal
Vektora : Jurnal Vektor dan Reservoir Penyakit Vol 10 No 1 (2018): Vektora : Jurnal vektor dan reservoir penyakit
Publisher : Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (B2P2VRP) Salatiga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.708 KB) | DOI: 10.22435/vk.v10i1.1054

Abstract

Fasciola Gigantica infection occurs in swamp buffalo on livestock in the swamp area of Hulu Sungai Utara District. Geographically, the livestock area is linked to the villages of Sungai Papuyu and Kalumpang Dalam by swamp water with no barrier. Such condition allows the snail as intermediate host of F. gigantica in livestock areas easily spread to residential areas and, therefore, raises the risk of transmission to humans. This study aimed to confirm the presence of F. gigantica in the cercariae form and snails in the area around the settlement of both villages. The study was an observational study of cross-sectional design, conducted in August-December 2014 . Snail sampling was conducted at the Sungai Papuyu and Kalumpang Dalam village using hand collection method. To find cercariae in the snail crushing technique was performed. Cercariae was confirmed by means of PCR to ensure they are F. gigantica in the Laboratory of Molecular Biology, Lambung Mangkurat University, Banjarbaru. The results in both villages showed 6 genera of snail, namely Pomacea, Bellamyâ , Indoplanorbis , Lymnaea , Gyraulus and Melanoides . There are 3 types of cercariae in the snails, namely Echinostome cercariae , Brevifurcate – pharyngeate cercariae and Sulcatomicrocercous cercariae. PCR shows positive samples of F. gigantica in the Echinostome cercariae form in Lymnaea and Indoplanorbis snails. This confirms the presence of F. gigantica cercariae around the settlements area and Lymnaea and Indoplanorbis snail are the first intermediate hosts.
Co-Authors Budi Hairani Mukhlis Zuardi Reni Herman Syarif Hidayat