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The Results of Toxoplasma gondii Examination IgM Antibodies Using ICT Method and ELISA Method in Pregnant Women Syifa Amelia Nadayang; Rizana Fajrunni'mah
Medical Laboratory Technology Journal Vol 6, No 1 (2020): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31964/mltj.v6i1.241

Abstract

Toxoplasmosis is a disease that can infect animals and zoonotic in humans. In pregnant women (congenital toxoplasmosis), the risk and severity of symptoms of congenital toxoplasmosis are more severe if infection occurs early in pregnancy and asymptomatic. The only way to determine disease is by serological screening. Serological diagnosis techniques for toxoplasmosis include ELISA (Enzyme-linked Immunosorbent Assay) and ICT (Immunochromatography Assay Test). However, until now, research on toxoplasmosis with the ICT method is still minimal. This study aims to analyze the results of the IgM antibody examination Toxoplasma gondii using the ICT method with the ELISA method and calculate the sensitivity and specificity of the ICT method and the ELISA method in pregnant women. The research design was used analytic (cross-sectional), with the number of samples is 33 serums. Data from the ELISA method obtained from secondary medical record data and the remaining serum samples were examined by the ICT method by researchers at the Harapan Kita Hospital Laboratory. In the ICT method, two samples (6.1%) were positive, and 31 samples (93.9%) were negative. In the ELISA method, results obtained similar to the ICT method, two samples (6.1%) positive and 31 samples (93.9%) with negative results. Toxoplasmosis examination can be done using the ICT method as the initial serological screening stage, but it not recommended as a diagnosis enforcer.
HASIL PEMERIKSAAN IMUNOKROMATOGRAFI COACTAIL ANTIGEN M.TUBERCULOSIS DAN METODE MOLEKULER PADA SUSPEK HIV Rizana Fajrunni'mah
Meditory : The Journal of Medical Laboratory Vol 8, No 1 (2020): Meditory, vol. 8 no 1 Juni 2020
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (358.66 KB) | DOI: 10.33992/m.v8i1.1093

Abstract

Background Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB) infection. Tuberculosis is one of the most common infections in people with HIV/ AIDS. Deaths due to TB in HIV patients occur due to delayed diagnosis. GeneXpert is a breakthrough discovery for TB diagnosis based on molecular examination using the semi quantitative Real Time Polymerase Chain Reaction Assay (RT-PCR) method. An examination has now been developed to detect MTB antigens using the method of rapid immunochromatography in the hope that it can be used as a means of supporting TB diagnosis better than detection of antibodies to MTB.Aim This study aims to compare the results of the TB GeneXpert RTPCR method with the rapid immunochromatography method in suspect HIV.Method This study uses a quantitative design with observational analytic design (cross sectional) with primary and secondary data collection.Result The results of this study found the value of sensitivity, specificity, accuracy, positive predictive value, negative predictive value of the ICT test in a row of 21%, 76%, 40%, 55.8%.Conclusion ICT TB has low sensitivity and sufficient specificity so that it is still not good if used for initial screening to detect pulmonary TB in suspect HIV.