Article Per Year (5 Year)
p-Index From 2019 - 2024
0.408
P-Index
This Author published in this journals
All Journal SEL Jurnal Penelitian Kesehatan Medical Laboratory Technology Journal
Paisal Paisal
Balai Litbang Kesehatan Tanah Bumbu
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Microfilaria Detection on Giemsa Blood Smears using Real-Time PCR Paisal Paisal; Erli Haryati; Dwi Candra Arianti; Muhammad Rasyid Ridha; Annida Annida
Medical Laboratory Technology Journal Vol. 5 No. 1 (2019): June
Publisher : Poltekkes Kemenkes Banjarmasin Jurusan Analis Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (495.21 KB) | DOI: 10.31964/mltj.v5i1.210

Abstract

Filariasis is an infectious disease caused by filarial worms, which found in many tropical and subtropical regions. In 2017, 12,677 cases of chronic filariasis were found in Indonesia, which 132 cases of them were from Central Kalimantan province. Data of Kapuas District Health Office shows 17 cases filariasis in 2015. Frequently, filariasis patients did not show any diseases symptoms, especially when the level of microfilariae in the blood is deficient. On the other hand, microscopic assay with Giemsa blood smears is still the gold standard to define filariasis. Thus, a false negative result may occur due to a low level of microfilariae in the blood. In this study, we develop a real-time PCR method, targeting the HhaI gene of filaria, to detect filarial worm from stored Giemsa blood taken from filariasis patients, in both dry and wet scraping methods. Our result shows that real-time PCR can detect Brugia malayi in all scraping samples, with Ct value from wet scraping sample tends to be higher than dry scraping. In conclusion, the real-time PCR method can be further used to define filariasis, especially in the condition when Giemsa smear blood cannot determine patient filariasis status.
PERILAKU MINUM OBAT MASSAL FILARIASIS DI DESA PIHAUNG DAN BANJANG, KABUPATEN HULU SUNGAI UTARA Paisal Paisal; Nita Rahayu; Annida Annida
Sel Jurnal Penelitian Kesehatan Vol 6 No 2 (2019): SEL Jurnal Penelitian Kesehatan
Publisher : Balai Penelitian dan Pengembangan Kesehatan Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.297 KB) | DOI: 10.22435/sel.v6i2.2233

Abstract

Sekitar 886 juta orang di dunia berisiko terkena filariasis dan membutuhkan obat pencegahan. Ada lebih dari 14 ribu orang di Indonesia menderita filariasis kronis pada 2014. Kasus filariasis di Kalimantan Selatan pada 2017 sejumlah 55 kasus, dengan kasus tertinggi ditemukan di Kabupaten Tabalong yaitu 30 kasus dan Kabupaten Barito Kuala sebanyak 16 kasus, sedangkan di Kabupaten Hulu Sungai Utara tidak dilaporkan adanya kasus. Kabupaten Hulu Sungai Utara sudah melakukan pemberian obat pencegahan massal sebanyak dua tahap. Penelitian ini bertujuan untuk melihat perilaku minum obat massal filariasis di Kabupaten Hulu Sungai Utara. Penelitian ini memiliki desain potong lintang. Waktu pengambilan sampel yaitu pada September 2017. Pengambilan sampel dilakukan di Desa Pihaung dan Desa Banjang. Rumah dipilih dengan teknik systematic random sampling. Semua orang yang ada dalam rumah, yang memenuhi kriteria sampel diwawancarai. Sebanyak 620 orang terpilih menjadi responden, terdiri dari 283 laki-laki dan 337 perempuan. Responden yang ikut pengobatan massal filariasis hanya 453 (73%) orang. Ada 40 (8,8%) responden yang tidak minum obat yang didapatnya, dengan alasan takut efek samping. Hampir seluruh responden minum obat di rumah (96,9%) dan diminum malam hari (91,8%). Kualitas pelaksanaan program pemberian obat pencegahan massal perlu diperbaiki agar responden mau minum semua obat yang didapatnya dan meminumnya di depan petugas kesehatan. Around 886 million people in the world are at risk for filariasis and need preventive medicine. In Indonesia, more than 14 thousand people were found suffering from chronic filariasis in 2014. In South Kalimantan, 55 cases were found in 2017, with the highest rates were in Tabalong Regency (30 cases) and Barito Kuala Regency (16 cases). Whereas in the Hulu Sungai Utara Regency, no cases were reported. Hulu Sungai Utara Regency has administered mass prevention drugs in two stages. This study aims to analyze the behavior of taking medication for filariasis in Hulu Sungai Utara Regency. The design of this study is cross sectional. The sample was taken in September 2017 which was done in Pihaung Village and Banjang Village. The house was chosen by systematic random sampling technique. All people in the house who met the sample criteria were interviewed. A total of 620 people was selected as respondents, consisting of 283 men and 337 women. Only 453 (73%) people took part in the mass treatment of filariasis. There were 40 (8.8%) respondents who did not take the medication they got, because they were worried about side effects. Almost all respondents took medication at home (96.9%) and were taken at night (91.8%). The quality of the implementation of the mass prevention drug administration program needs to be improved so that respondents want to take all the drugs they get and take them in front of the health worker.
Co-Authors Annida Annida Dwi Candra Arianti Erli Haryati Muhammad Rasyid Ridha Nita Rahayu