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The Existence of Endophytic Actinobacteria from Rhododendron zoelerri Revealed by Culture-Dependent and Culture-Independent Approaches Yulin Lestari; Lia Aseptin Murdini; Dedy Duryadi Solihin
HAYATI Journal of Biosciences Vol. 25 No. 2 (2018): April 2018
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (559.98 KB) | DOI: 10.4308/hjb.25.2.54

Abstract

Endophytic actinobacteria from medicinal plant may play a significant role in producing bioactive compounds. The information regarding their diversity is an important.  Rhododendron are traditionally used for treating human disorders. One of the selected Rhododendron used in this study was R.  zoelleri from Papua origin, which has been conserved and grown in Cibodas Botanical Garden, West Java, Indonesia. The aim of this study was to assess the existence of endophytic actinobacteria from R. zoelleri based on a culture-dependent and their community structure based on a culture-independent approach. Culturable actinobacteria were isolated and cultured on HV medium. Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) targeting the metagenomic 16S rRNA was used to analyse the structure of the actinobacterial community. Six culturable endophytic actinobacteria (200 cfu/g fresh weight) from R. zoelleri were successfully isolated, three isolates from leaf, and the other isolates were obtained from stem. The six culturable isolates were RZP 1.3, RZP 1.1, RZP 2.2, RZPB 1.1, RZPB 7.1, RZPB 4.1. Based on their morphological characteristics, the endophytes have Streptomyces characters. The existence of Streptomyces spp. were also confirmed with molecular analysis based on 16S rRNA gene. The phylogenetic analysis based on 16S rRNA gene to the reference strains available in EzTaxon-e database showed that six isolates were closely related to S. djakartensis strains of NBRC 15409ᵀ(99.19%), S. tritolerans strains of DAS 165T(99.90%), S. coelicoflavus strains of NBRC 15399T(99.59). However, they showed differences in morphological characteristics as compared with the reference strains. The metagenomic analysis of the DGGE profile based on 16S rRNA gene showed the community structure of endophytic actinobacteria from R. zoelleri which was represented by 13 DGGE bands. The bands were closely related to Agromyces, Gordonia, Microbacterium, Micromonospora, Propionibacterium, Saccharomonospora, Streptomyces which have 93.18%-100% similarity. Based on the data, it showed diversity of endophytic actinobacteria from R. zoelleri which may be further assess for their novelty and bioprospecting.
DNA Barcode of Seven Indonesian Hornbills Species (Aves: Bucerotidae) Based on Mitochondrial DNA Cytochrome Oxidase Subunit I Jarulis Jarulis; Dedy Duryadi Solihin; Ani Mardiastuti; Lilik Budi Prasetyo
HAYATI Journal of Biosciences Vol. 25 No. 4 (2018): October 2018
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.707 KB) | DOI: 10.4308/hjb.25.4.178

Abstract

DNA barcoding based on mitochondrial DNA COI gene is very useful in identifying of Indonesian hornbill. We sequenced the DNA barcode of seven hornbill species using the mitochondrial DNA COI gene to explore their genetic variation, identity, distance, and phylogenetic. Thirty-one blood samples from seven hornbill species were isolated and analyzed. Slight variation was observed within the nucleotide of the hornbill species. In contrary, fairly significant difference was shown within the genus and family level. COI gene sequences generated from this study, showed unmatched result with BoLD System database. These seven Indonesian hornbill species were then divided into two groups, namely Group I consisting of Aceros cassidix, Rhyticeros plicatus, R. undulatus, Buceros rhinoceros, and B. bicornis, while Group II occupied by Anthracoceros albirostris and A. malayanus; both groups with genetic distance 5.90%. Overall in-group had 9.40% distances to the hornbill used as the out group. COI sequence gene from these seven hornbill species are novel for identifying Indonesian hornbills. We encourage its use as quick species identification, applied to prevent illegal poaching conservation management.
Habitat Suitability Modeling of Bornean Orangutan (Pongo pygmaeus pygmaeus) in Betung Kerihun National Park, Danau Sentarum and Corridor, West Kalimantan Hari Prayogo; Achmad Machmud Thohari; Dedy Duryadi Solihin; Lilik Budi Prasetyo; Jito Sugardjito
Jurnal Manajemen Hutan Tropika Vol. 20 No. 2 (2014)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (989.61 KB) | DOI: 10.7226/jtfm.20.2.112-120

Abstract

Conservation areas such as national parks became the last safe area for biodiversity in Indonesia, especially for orangutan, because they have permanent status, and other forest areas may change at any time. Currently, there have been tremendous numbers of land use changes in orangutan habitats such as the development of roads, large scale plantations, settlements, farming as well as logging.  However, the available national parks still offer secure places for orangutans. This research was conducted to study the impact of spatial land use policy on the habitat distribution of orangutans. A remote sensing technology was used to study the habitat distribution and suitability of orangutan in Betung Kerihun National Park (BKNP), Danau Sentarum National Park (DSNP) and a corridor which connects these 2 parks. Seven parameters have been observed to characterize the habitat of orangutans. Habitat suitability levels of three research sites i.e. BKNP, corridor, and DSNP were 53.72%, 42.94%, and 3.34% which is considered as high, moderate, and low level of habitat suitability, respectively. These results were obtained with 93.63% of validation.
Respons Fisiologis Ayam KUB (Kampung Unggul Balitbangtan) dan Ayam Walik dengan Haplotipe Gen HSP70 Berbeda yang Terpapar Cekaman Panas Akut Any Aryani; Dedy Duryadi Solihin; Cece Sumantri; Rudi Afnan; Tike Sartika
Jurnal Ilmu Pertanian Indonesia Vol. 26 No. 2 (2021): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.26.2.276

Abstract

This study was conducted to determine the physiological response of acute heat stress in Kampung Unggul Balitbangtan (KUB) and Walik chickens with four different HSP70 gene haplotypes (H1', H1, H2, and H3). Acute heat stress is carried out at 35°C for one hour. The data was analyzed based on rectal temperature measurements, distribution of body surface temperature (head, neck, body, and leg area), hormone levels (corticosterone and triiodothyronine), and time of panting. Body surface temperature is detected using infrared thermography. The results showed that haplotype H1 Walik chicken is more susceptible to heat stress based on the rectal temperature. In contrast, H1' haplotype Walik chicken released more heat through the body surface in the leg area. Keywords: heat stress, hormone, KUB chicken, infrared thermography, walik chicken
Subterranean termite, Macrotermes, play important role on plant nutritive cycles through disintegration and decomposition of organic matter processes.  In the other hand, subterranean termite can cause wooden damage in the nature and human dormitory as well. Therefore, Macrotermes are potential pest and need to be controlled.  Effective pest-control of subterranean termite can be made when their species status and distribution are well known. This research is aimed to identify the status of spec Niken SUBEKTI; Dedy DURYADI; Dodi NANDIKA; Surjono SURJOKUSUMO; Syaiful ANWAR
Jurnal Ilmu dan Teknologi Hasil Hutan Vol. 1 No. 1 (2008): Jurnal Ilmu Teknologi Hasil Hutan
Publisher : Departemen Hasil Hutan, IPB

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Abstract

Subterranean termite, Macrotermes, play important role on plant nutritive cycles through disintegration and decomposition of organic matter processes.  In the other hand, subterranean termite can cause wooden damage in the nature and human dormitory as well. Therefore, Macrotermes are potential pest and need to be controlled.  Effective pest-control of subterranean termite can be made when their species status and distribution are well known. This research is aimed to identify the status of species and the dispersal of Macrotermes at their natural habitat in order to explore natural bio-resources richness and as an effort towards effective pest-control due to increasing risk of attack.  Survey method was used termite samples were collected at four different locations which are defined by global positioning system (GPS).  Nest classification is according to Meyer et al. (2003). The nest mapping is based on the elevations of their natural habitat in Gunung Halimun Salak National Park (900-1000 asl), and (600-700 asl), Yanlappa sanctuary (200-300 asl), and Ujung Kulon Nasional Park (0-100 asl). Phylogenetic relationships analysis showed that subterranean termite Macrotermes in the natural forest community are belongs to one big group Macrotermes gilvus Hagen. Based on nest size, the colony of Macrotermes can be classified into three different sizes: large, medium, and small nest.  Large and medium nest can be found in large amount in Yanlappa sanctuary (15 and 23 colonies, respectively) and the majority of small nest occurred in Gunung Halimun Salak National Park at 900 asl (78 colonies). Temperature and humidity is the most environmental factor that can influence on termite and other factor is rain fall, soil structure, and plant vegetation.   Keywords:  Distribution, identify, Macrotermes gilvus Hagen, nest
Genetic Diversity and Phylogenetic Relationship of Kryptoterus limpok and Kryptopterus apogon from Kampar and Indragiri River Based on Cytochrome b Gene Roza Elvyra; Dedy Duryadi Solihin; Ridwan Affandi; Zairin Junior; Yusnarti Yus
Jurnal Ilmu-Ilmu Perairan dan Perikanan Indonesia Vol. 16 No. 1 (2009): Juni 2009
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.833 KB)

Abstract

The utility of cytochrome b gene as a molecular marker to obtain genetic diversity and phylogenetic relationship among Kryptopterus spp. consist of Kryptopterus limpok and Kryptopterus apogon from Riau Province has been studied. The universal primers of (F) L14841 and (R) H15149 were used to amplify the cytochrome b gene. The results of multiple allignment were 159 nt (coding 53 amino acids). Kryptopterus limpok from Kampar and Indragiri River form a phylogeny cluster at 80% bootstrap value. Kryptopterus apogon from Kampar and Indragiri River form a phylogeny cluster at 99% bootstrap value.Keywords : cytochrome b, Kryptopterus limpok, Kryptoperus apogon.
Bacteria are microbes which have an ability to live wherever there is a life.  Some of the bacteria are saprophyte and some are parasitic. But most of the bacteria have not been identified or cultured; therefore the benefits are still unknown.  Methanogenic bacteria are one of the saprophyte bacteria. These bacteria produces methane, a biogas as an alternative fuel in the future.  Most of methanogenic bacteria are uncultured, however a few of them are found in the sewage of coconut water.  The o Ardi Kapahang; Maria Bintang; D.D. Sastraatmadja; Dedy Duryadi Solichin
Forum Pasca Sarjana Vol. 30 No. 1 (2007): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Abstract

Bacteria are microbes which have an ability to live wherever there is a life.  Some of the bacteria are saprophyte and some are parasitic. But most of the bacteria have not been identified or cultured; therefore the benefits are still unknown.  Methanogenic bacteria are one of the saprophyte bacteria. These bacteria produces methane, a biogas as an alternative fuel in the future.  Most of methanogenic bacteria are uncultured, however a few of them are found in the sewage of coconut water.  The objectives of this research are to isolate, characterize and identify the methanogenic bacteria that lived in coconut water.  The method of this research was fermentation, analysis characterization, and identification of methanogenic bacteria. First, methanogenic bacteria were isolated from coconut water by fermentation.  The samples were from four places in Minahasa, which are Rasi (I), Koka (II), Amurang (III), and Lola (IV) and one place in Bogor (V). Secondly, the methane produced from fermentation was analysed by gas chromatography and the bacteria can be characterized by Bergey’s method. The next step is the identification which was conducted by isolating  the DNA, amplifying the DNA by PCR, then sequencing the DNA with BioEdit Sequence Aligment.  As the result, high and stable  methane was produced in Rasi (I) and Amurang (III). The characteristic of the bacteria are red colony (M) and white colony (P). The shape of the colonies is circles, gram positive, basil shape, mesofile, positive of catalase and citrate, positive of sugar fermentation, gelatin, casein and starch hydrolysis also lived in Nutrient Broth with pH 5.7-6.8.  The sequencing of isolate P resulting in nucleotide  composition of G 31.25%, C 20.58%, A 27.11% and T 21.04%  while isolate M are   G 31.34%, C 20.31%, A 27.02% and T 21.32%.  The identification of isolate M is equal with Clostridium tyrobutyricum (100 %) and isolate P is very close with Clostridium tyrobutyricum (99 %).   Key words: bacteria, methane, coconut water
Community Structure of Fish and Environmental Characteristics in Cisadea River, West Java, Indonesia Epa Paujiah; Dedy Duryadi Solihin; Ridwan Affandi
Jurnal Biodjati Vol 4, No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.6096

Abstract

 Environmental characteristics can influence the distribu-tion of animal communities including fish in aquatic habitats such as a river. The study of fish communities in Cisadea River aimed to learn the relation between the fish community structure and the environmental characteristics of the river itself. This research was conducted in Cisadea River, Cianjur Regency, West Java, Indone-sia during dry season from July 2012 until February 2013. Samples of fish were caught using active fishing gear (Electrofishing Gear, spreading nets, scoop, lift net) and passive fishing gear (trap). The results showed that the quality of the physical chemistry of Cisadea waters was still in the normal range that could support fish life. The fish community in Cisadea River consisted of 11 orders, 26 families, 35 genera and 48 species. During the study, Rhyacichthys aspro (19,85%) and Glyptothorax plathypogon (20,15%) were the domi-nant species caught. The diversity index value (H’) was 2.87 (mid-dle category) with the dominance value of 0.09 (low category) and evenness value of 0.74 (high category). Moreover, community simi-larities according to the presence of species made the LC1 and LC2 locations in one group while the LC3 locations in a separate group. 
Permodelan Kesesuaian Habitat Orangutan Kalimantan (Pongo pygmaeus pygmaeus) di Koridor Satwa Kapuas Hulu Kalimantan Barat Hari Prayogo; Thohari Thohari; Achmad Machmud; Solihin Solihin; Dedy Duryadi; Prasetyo Prasetyo; Lilik Budi; Sugardjito Sugardjito; Jito Jito
Jurnal Penelitian Hutan dan Konservasi Alam Vol 13, No 2 (2016): Jurnal Penelitian Hutan dan Konservasi Alam
Publisher : Pusat Penelitian dan Pengembangan Hutan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jphka.2016.13.2.137-150

Abstract

ABSTRACTKapuas Hulu, as conservation districts, established regional wildlife corridor that connected Betung Kerihun and Danau Sentarum National Park as a Strategic Area District which highlight aspects of the environment. This wildlife corridor holds a prominent role in the movement of animals, especially orangutans of both national parks. This research was conducted to identify the impact of land use policies on the distribution of orangutans in the corridor. Although it has been designated as a wildlife corridor, many land conversion disconnecting wildlife corridors such as road construction, large-scale plantations development, land clearing for settlement, cultivation, and deforestation. However, the two national parks still offers a secure place for orangutans. A remote sensing technology was used to map the distribution and habitat suitability for the orangutan in the wildlife corridor. Seven parameters were observed to study the habitat of orangutans. The results revealed that the habitat suitability level of wildlife corridor was 49.94%, 46.61% and 3.46% for high, moderate and low level of suitability respectively. The results were supported by validation of 32.29% and 67.71% for moderate and high suitability respectively.Key words : Corridor, habitat, orangutan, wildlife suitabilityABSTRAKKabupaten Kapuas Hulu sebagai kabupaten konservasi telah menetapkan daerah koridor satwa yang menghubungkan Taman Nasional Betung Kerihun dan Taman Nasional Danau Sentarum sebagai Kawasan Strategis Kabupaten yang menonjolkan aspek lingkungan. Koridor satwa ini memiliki peranan yang penting bagi pergerakan satwa terutama orangutan dari kedua taman nasional ini. Studi ini dilakukan untuk memahami dampak tata guna lahan terhadap sebaran orangutan, di koridor satwa. Pembukaan jalan, perkebunan skala besar, pembukaan lahan untuk pemukiman, perladangan serta penebangan hutan telah menjadi penyebab terputusnya habitat orangutan. Wilayah yang masih aman sebagai habitat orangutan adalah di dalam kawasan taman nasional. Penelitian ini dilakukan menggunakan teknologi penginderaan jarak jauh untuk memetakan sebaran dan kesesuaian habitat orangutan di kawasan koridor satwa. Tujuh parameter habitat orangutan digunakan dalam analisis spasial kesesuaian habitat. Dari hasil penelitian ini didapatkan bahwa kawasan koridor memiliki tingkat kesesuaian habitat yang tinggi sebesar 49.94%, tingkat kesesuaian sedang sebesar 46.61% dan kesesuaian yang rendah sebesar 3.46%. dan hasil ini ditunjang dengan besaran nilai validasi untuk kelas kesesuaian sedang sebesar 32.29% dan kelas kesesuaian tinggi sebesar 67.71%.Kata kunci : Habitat, kesesuaian, koridor, orangutan, satwa
Optimasi Rendemen Fibroin Ulat Sutera Bombyx mori L. dan Attacus atlas L. dengan Response Surface Methodology Yuni Cahya Endrawati; Dedy Duryadi Solihin; Ani Suryani; Subyakto Subyakto
agriTECH Vol 37, No 2 (2017)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2701.524 KB) | DOI: 10.22146/agritech.10497

Abstract

The fibroin extraction process was done base on temperature and time parameters without knowing exactly the rendement been maximum or not. Optimum rendement data are critical to the business feasibility for scaling up to the industrial level. The objective of this research is to obtain the optimum extraction process (degumming) to get the highest fibroin rendement. The method in this research was response surface method (RSM) where NaOH concentration, process temperature and time consumption were chosen to be independent variables. These variables were formulated in the central composite design (CCD) research method at RSM Program with the center point is NaOH 0.1 N, 105 °C for 30 minutes. The weight of fibroin yield (%) being use as a response condition (dependent variable) to get the optimum result of fibroin rendement. The optimum degumming process condition of Bombyx mori are achieved where the NaOH 0.018 N, 110.53 °C temperature degree for 55.51 minutes for a batch time process with the rendement recorded was 71.11 ± 0.98 %. As for “Attacus atlas” the optimum rendement was 83.06 ± 1.50 % achieved at 0.12 N NaOH concentration, process temperature was 79 oC and 42.65 minutes for twice degumming. The best selected fribroin degumming method will lead to the optimum yield achievement. ABSTRAKEkstraksi fibroin dilakukan dengan perlakuan suhu dan waktu tanpa diketahui maksimal atau tidaknya capaian rendemen yang dihasilkan. Padahal data tersebut sangat penting untuk estimasi profitabilitas pada usaha hilir ulat sutera. Penelitian ini bertujuan untuk mendapatkan formulasi ekstraksi (degumming) yang menghasilkan rendemen fibroin optimum. Metode yang digunakan adalah optimasi ekstraksi fibroin menggunakan Program Response Surface Methodology (RSM) dengan tiga variabel bebas, konsentrasi NaOH, suhu dan waktu. Variabel tersebut diformulasikan dalam rancangan percobaan Central Composite Design (CCD) di Program RSM menggunakan titik pusat NaOH 0,1 N, suhu 105 °C selama 30 menit. Pada rancangan percobaan tersebut, rendemen bobot fibroin (%) digunakan sebagai respon (variabel terikat) untuk menghasilkan kondisi optimum. Kondisi tersebut berupa formulasi variabel bebas yang mengoptimumkan rendemen fibroin. Kondisi optimum Bombyx mori dicapai pada konsentrasi NaOH 0,018 N, suhu 110,53 °C dan waktu 55,51 menit pada satu kali degumming dengan perolehan rendemen fibroin sebesar 71,11 ± 0,98 %. Rendemen fibroin optimum sebesar 83,06 ± 1,50 % pada Attacus atlas dihasilkan dari kondisi optimum NaOH 0,12 N, suhu 79 °C selama 42,65 menit pada dua kali degumming. Metode ekstraksi fibroin yang tepat akan menghasilkan rendemen fibroin yang optimum. 
Co-Authors Abdul Rahman Singkam Achmad - Taher ACHMAD FARAJALLAH Achmad Machmud Achmad Machmud Thohari Achmad Taher Achmad Taher Achmad, Taher Agus Nuryanto Agus Wahyana Anggara Agus Wahyana Anggara Agus Wahyana Anggara, Agus Wahyana Alam Putra Persada Ani Mardiastuti Ani Mardiastuti Ani Suryani Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R Any Aryani Ardi Kapahang Arief Boediono Arzyana Sunkar Bahiyah Bahiyah Bambang Purwantara Cece Sumantri CECILIA ANNA SEUMAHU CHRISTIAN HANSJOACHIM SCHULZE D.D. Sastraatmadja DAMAYANTI BUCHORI Dedi . Soedharma Dedi Soedharma DEWI APRI ASTUTI Dewi Elfidasari Dewi Malia Prawiradilaga Dewi Malia Prawiradilaga, Dewi Malia Diah Iskandriati DIAH ISKANDRIATI DIETMAR BLOHM Dodi Nandika Dondin Sajuthi Dwi ASTUTI Dwi Astuti Dwi Sendi Priyono DYAH PERWITASARI -FARAJALLAH Epa Paujiah, Epa Evy Ayu Arida EVY AYU ARIDA Fahma Wijayanti FAHRI FAHRUDIN Findra, Muhammad Nur FUNGKEY HOETAMA Gita Kusuma Rahayu Hadi Allikodra Hadi S Alikodra Hadi S Alikodra HADI SUKADI ALIKODRA HAJRIAL ASWIDINNOOR Handayani Handayani Hari Prayogo Harini Nurcahya Mariandayani Heddy Julistiono HEDDY JULISTIONO Hermanu Triwidodo I Gusti Agung Arta Putra I WAYAN SUANA Ibnu Maryanto Iman Rusmana Irma Shita Arlyza Irzaman, Irzaman Isdradjad Setyobudiandi Ismayati Afifah Jamhari Jamhari Jarulis Jarulis Jarulis Jarulis Jarulis Jarulis Jito Jito Jito Sugardjito Joko Pamungkas Jusmaldi Jusmaldi Kadarwan Soewardi Khustina, Yenny Chusna Khustina, Yenny Chusna Kunio Watanabe Lia Aseptin Murdini Lilik Budi Lilik Budi Prasetyo Lilik Budi Prasetyo Lucia Johana Lambey M F Rahardjo M. F. Rahardjo M. Zairin Junior MARIA BINTANG MF Rahardjo Mustafa Sabri Nastiti Kusumorini NEVIATY PUTRI ZAMANI Niken Subekti Niken TM Pratiwi Nurlisa Butet, Nurlisa Prasetyo Prasetyo Priyono, Siti N. Retno Damayanti Soejoedono RICHARD F GRANT Ridwan Affandi Ridwan Affandi RIDWAN AFFANDI Ridwan Affandi Ridwan Affandi Ridwan Affandi Ridwan Affandi Ridwan Affandi Rini Widayanti Robba Fahrisy Darus ROEDHY POERWANTO Roedy Poerwanto Roedy Poerwanto Roni Koneri Ronny Rachman Noor Roza Elvyra Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudhy Gustiano Rudi Afnan Rudi Tarumingkeng Rudy C Tarumingkeng Safrida Safrida Saroyo Saroyo SATA YOSHIDA SRIE RAHAYU SATRIYAS ILYAS SELA SEPTIMA MARIYA SILMI MARIYA Siti N. Priyono SJAFRIDA MANUWOTO Soaloon Sinaga Soaloon Sinaga Sobir Sobir Sobir Sobir Sobir Sobir Solihin Solihin Sri Catur Setyawatiningsih Sri Catur Setyawatiningsih sri murtini . SRI NINGSIH Sri Sulandari Sri Supraptini Mansjoer Subyakto Subyakto Sugardjito Sugardjito Suharsono Suharsono SULISTIYANI SULISTIYANI SULISTIYANI SULISTIYANI Sulistiyani Sulistiyani Sulistiyani, Sulistiyani Surjono Surjokusumo Syaiful Anwar Syamsul Bachry Tedjo Sukmono Thohari Thohari Tike Sartika Tri Haryoko Tri Haryoko, Tri UUS SAEPULOH UUS SAEPULOH Wasmen Manalu Yuli Wahyu Tri Mulyani YULIN LESTARI Yuni Cahya Endrawati Yus Rusila Noor Yusnarti Yus