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Purifikasi Kolagenase dari Usus Bandeng (Channos channos, Forskal) Yuniarti, Tatty; Nurhayati, Tati; Jacoeb, Agoes Mardiono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.577 KB) | DOI: 10.24002/biota.v15i3.2593

Abstract

Collagenase was purified from intestines of milkfish (Chanos chanos, Forskal) by extraction,ammonium precipitation, ion exchange chromatography on DEAE Sephadex A-50 and gelfiltration on a Sephadex G-100 column. The purification and yields were 114.731 fold and1.26% when compared to those in the starting-crude extract. The molecular mass of milkfishserine collagenase was estimated to be 14.63 kDa and 27.46 kDa.
PERUBAHAN KOMPONEN SERAT RUMPUT LAUT Caulerpa sp. (DARI TUAL, MALUKU) AKIBAT PROSES PEREBUSAN Nurjanah; Agoes Mardiono Jacoeb; Taufik Hidayat; Rudy Chrystiawan
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 10 No. 1 (2018): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (298.11 KB) | DOI: 10.29244/jitkt.v10i1.21545

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Rumput laut Caulerpa sp. dikenal sebagai sumber serat. Rumput laut Caulerpa sp. banyak ditemukan di pesisir pantai Indonesia, salah satunya di perairan Tual, Maluku Tenggara. Rumput laut ini sering dikonsumsi segar oleh masyarakat pesisir ataupun direbus terlebih dahulu. Perebusan bertujuan untuk menginaktifkan enzim dan menurunkan jumlah mikroba, namun dikhawatirkan dapat mempengaruhi komponen serat yang terkandung pada rumput laut. Penelitian ini bertujuan untuk menentukan perubahan kadar serat pangan (serat pangan larut dan tak larut), serat kasar, dan komponen serat (selulosa, hemiselulosa, lignin) akibat proses perebusan pada suhu 90ºC selama 5 menit. Analisis uji yang dilakukan, diantaranya analisis proksimat, kadar karbohidrat metode Luff schoorl, serat pangan metode enzimatis, serat kasar, dan komponen serat (selulosa, hemiselulosa, lignin). Hasil penelitian menunjukkan perebusan tidak mempengaruhi kadar lignin. Perebusan meningkatkan kadar serat pangan tak larut 1,25%, selulosa 13,91%, hemiselulosa 11,4%, dan menurunkan kadar serat pangan total 3,8%, serat pangan larut 5,05%, dan serat kasar 0,85%.
PEMANFAATAN KUNYIT (Curcuma domestica Val) DAN JERUK NIPIS (Citrus aurantifolia Swingle) DALAM PEMBUATAN ABON IKAN LEMURU Djoko Poernomo; Agoes Mardiono Jacoeb; Uut Tri Utami; Roni Nugraha
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 13 No 2 (2010): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1435.336 KB) | DOI: 10.17844/jphpi.v13i2.5352

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The research objectives were determine the concentration of lime and soaking time that could reduce the smell of sardine, the concentration of curcumin that could prevent the oxidation of fish, and the effect of curcumin on chemical and microbiology characteristic of sardine abon during 30 days storage. Sardine was soaked in lime solution with concentration from 5 to 20% (v/v) for 10, 20, and 30 minutes. After the best concentration of lime was found, the experiment was continued to determine the optimum concentration of curcumin in maintained he chemical and microbiology characteristic of sardine abon by adding the fish with curcumin from 1% to 5% (w/w). The abon was stored for 30 days andevery 10 days the sensory test, proximate analysis, analysis of water activity (aw), TPC and TBA were carried out. Sensory test showed that the addition of lime until 15% could increase the result, however, the sensory test result depleted when 20% of lime was used. The period of immersion also gave positive correlation with sensory test. The preliminary experiment also showed that the addition of curcumin increased the value of sensory test with 1 and 2% of curcumin gave better result compared with 3, 4 and 5% curcumin. During 30 days storage period, the value of sensory parameters and proximate properties were decrease, meanwhile, the water activity, TPC and TBA were rised. In conclusion, up to 30-day storage period, teh quality of fish abon was decrease,d but still could be accepted by the panelists. Immersion of fish abon on 15% of lime for 30 minutes and addition of 2% curcumin gave better quality of fish abon compared with others treatmen with value of water content 10.71%, ash4.16%, fat 26.82%, protein 30.51%, carbohydrate 27.80%, aw 0.51, TPC 2.45 colonies/ gram, and TBA 1.76mg/kg. The addition of curcumin fish abon did not significantly influence the appearance, water content, ash, fat, and carbohydrates, however the color, aroma, texture, flavor, protein content, water activity (aw), value TBA, and TPC significantly influenced.Keywords: curcumin, fish abon, lime, sencory parameters
HUBUNGAN PANJANG BOBOT DAN KEBIASAAN MAKAN IKAN BUNTAL PISANG (Tetraodon lunaris) DI PERAIRAN KABUPATEN CIREBON Pratama, Ginanjar; Nurjanah, Nurjanah; Suwandi, Ruddy; Jacoeb, Agoes Mardiono
Dinamika Maritim Vol 6 No 2 (2018): Dinamika Maritim, Vol. 6 No. 2, February 2018
Publisher : Coastal and Marine Resources Research Center, Raja Ali Haji Maritime University, Tanjungpinang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (614.793 KB)

Abstract

PENGARUH CAHAYA TERHADAP AKTIVITAS METABOLISME IKAN LELE DUMBO (CLARIAS GARIEPINUS) PADA SIMULASI TRANSPORTASI SISTEM TERTUTUP Ruddy Suwandi; Agoes Mardiono Jacoeb; Vickar Muhammad
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 14 No 2 (2011): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.392 KB) | DOI: 10.17844/jphpi.v14i2.5317

Abstract

Consumer demand for commodities is growing, especially for the type of fish that have high economis value, one of which is dumbo catfish (Clarias gariepinus). Handling of the transport system is needed to keep fish alive until the destination. The purpose of this research was to study the effect of light intensity on simulated fish either at rest or moving. The study was conducted with simulation of a closed system transportation for six hours and measuring the water quality each hour. The treatment used were a light simulation, dark simulation, non-simulated light, and dark non-simulation. DO values decrese from 5,0175 ppm to 2,3812 ppm after 6 hours transportation. Water temperature during research at 27,8-29,4˚C. PH values in the study range from 7,2 to 6,3 ppm. The values of carbondioxide increased during transportation. The highest concentration of ammonia presented after six hours in all treatment.The best treatment of this study was non-simulated light.Keywords : Catfish (Clarias gariepinus), closed system transport, water quality
KANDUNGAN ASAM LEMAK DAN KOLESTEROL KAKAP MERAH (Lutjanus bohar) SETELAH PENGUKUSAN agoes mardiono jacoeb; - - nurjanah; Aninta - Saraswati
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 16 No 2 (2013): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (410.188 KB) | DOI: 10.17844/jphpi.v16i2.8051

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Ikan kakap merah (Lutjanus bohar) merupakan salah satu ikan ekonomis tinggi yang banyak terdapatdi perairan Indonesia. Tujuan penelitian ini adalah menentukan pengaruh pengukusan terhadap komposisikimia, karakteristik asam lemak, kandungan kolesterol, dan struktur jaringan daging ikan kakap merah.Ikan kakap merah sebelum proses pengukusan mengandung 77,53% air, 1,42% abu, 20,55% protein, 0,27%lemak, dan 0,23% karbohidrat. Setelah proses pengukusan, ikan kakap merah mengandung 76,83% air, 1,48%abu, 20,78% protein, 0,05% lemak, dan 0,86% karbohidrat. Pengukusan yang dilakukan mengakibatkanpenyusutan terhadap kandungan asam lemak daging ikan kakap merah. Ikan kakap merah memiliki rasioPUFA/SFA 0,97 pada kondisi segar dan 0,64 setelah pengukusan. Rasio asam lemak n-3/n-6 6,25 padakondisi segar dan 2,43 setelah pengukusan. Kandungan kolesterol pada daging ikan kakap merah mengalamipenyusutan setelah proses pengukusan dari 95,5 mg/100g menjadi 24,2 mg/100g. Struktur jaringan dagingikan kakap merah setelah proses pengukusan tampak lebih kompak karena mampu mempertahankan dayaawetnya.Kata kunci: asam lemak, ikan kakap merah, kolesterol, Lutjanus bohar, struktur jaringan
KOMPOSISI KIMIA, KOMPONEN BIOAKTIF DAN AKTIVITAS ANTIOKSIDAN BUAH LINDUR (Bruguiera gymnorrhiza) Agoes Mardiono Jacoeb; Pipih Suptijah; Zahidah mawardi
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 16 No 1 (2013): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.276 KB) | DOI: 10.17844/jphpi.v16i1.7772

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Tanaman lindur (Bruguiera gymnorrhiza) merupakan salah satu tumbuhan mangrove yang biasadimanfaatkan sebagai makanan dan obat tradisional, namun masih belum cukup informasi sebagaisumber antioksidan. Tujuan penelitian ini adalah menentukan komposisi kimia, komponen bioaktif,aktivitas antioksidan, dan kemampuan ekstrak buah lindur dalam menghambat pembentukan bilanganperoksida. Ekstraksi yang digunakan adalah ekstraksi bertingkat dengan tiga jenis pelarut yang memilikitingkat kepolaran berbeda, yaitu n-heksana, etil asetat, dan metanol. Penentuan aktivitas antioksidandilakukan dengan menggunakan metode 1,1-diphenyl-2-picrylhidrazyl (DPPH). Buah lindur memilikikadar air 62,92%, kadar abu 1,29%, kadar lemak 0,79%, kadar protein 2,11% dan kadar karbohidrat32,91%. Hasil ekstraksi menunjukkan bahwa rendemen ekstrak kasar tertinggi dihasilkan oleh ekstraksidengan metanol, diikuti ekstraksi dengan etil asetat, dan n-heksana. Ekstrak metanol memiliki aktivitasantioksidan sangat kuat dengan nilai IC50 sebesar 9,42 ppm. Ekstrak etil asetat dan n-heksana memilikiaktivitas antioksidan sangat lemah dengan nilai IC50 sebesar 443,61 ppm dan 2256,13 ppm. Uji fi tokimiamenunjukkan bahwa ekstrak yang memiliki aktivitas antioksidan terbaik mengandung steroid, fl avonoid,dan tanin. Konsentrasi ekstrak yang memiliki daya hambat terbaik terhadap pembentukan peroksidapada emulsi minyak adalah pada konsentrasi 250 ppm dengan bilangan peroksida sebesar 0,21 Meq/kg.
KARAKTERISTIK PROTEIN DAN ASAM AMINO DAGING RAJUNGAN (Portunus pelagicus) AKIBAT PENGUKUSAN Agoes Mardiono Jacoeb; Nurjanah .; Lenny Asnita Br Lingga
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 15 No 2 (2012): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.211 KB) | DOI: 10.17844/jphpi.v15i2.6207

Abstract

Crabs (Portunus pelagicus) is one member of the class Crustacea that being a major exported commodity. It is exported mostly in form frozen without head and shell and canned pruduct. Steam process is one important step in crab’s canning industry. This research aimed to study the yield, proximate composition, water-soluble protein, salt-soluble protein, amino acids content and the structure of crabs meat’s tissue analysis in a fresh and after steam process. Fresh crabs has higher yield than the steamed one. Steamed crab’s yield decreased by 14.99%. Proximate composition of fresh and steamed crabs as follows, moisture (wb) 78.47% and 75.43%, ash (db) 7.66% and 6.02%; protein (db) 68.09% and 66.63%; fat (db) 0.84%   and 0.75%, charbohydrate (db) 23.41% and 26.62%; water-soluble proteins (db) 40.87% and 25.32%; salt-soluble proteins (db) 58.06% and 30.77%. Crabs meat contained 15 amino acids, 9 essential amino acids and 6 non essential amino acids. The highest essential amino acid composition was arginin by 6.87% in fresh crab and 6.80% in steamed crab, while the highest non essential amino acid composition was glutamate by 10.92% in fresh crab and 9.81% in steamed crab. Fresh crab meat has connective fibers, while the steamed crab meat has  disjointed and not compact fibers.Key words: amino acids, crab (Portunus pelagicus), meat tissue, protein, steaming.
PEMBUATAN EDIBLE FILM DARI PATI BUAH LINDUR DENGAN PENAMBAHAN GLISEROL DAN KARAGINAN agoes mardiono jacoeb; Roni - Nugraha; siluh putu sri dia utari
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 17 No 1 (2014): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2320.3 KB) | DOI: 10.17844/jphpi.v17i1.8132

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Buah lindur (Bruguiera gymnorrhiza) mengandung karbohidrat yang tinggi dan belum dimanfaatkan secara optimal. Penelitian ini bertujuan untuk menentukan formulasi edible film dari buah lindur dan mengkarakterisasi produk yang dihasilkannya. Edible film dibuat dengan menambahkan tepung pati buah lindur sebesar 4%; perlakuan gliserol 1% dan 1,5%; dan konsentrasi karaginan 2%, 2,5% dan 3%. Nilai ketebalan edible film yang dihasilkan dari keenam formula berkisar 0,13-0,20 mm sedangkan nilai kuat tarik berkisar 132,88-168,33 kgf/cm2. Nilai persen pemanjangan berkisar 177,7-181,21% dan nilai laju transmisi uap air berkisar 231,23-298,82 g/m2/24 jam. Pati buah lindur sangat potensial untuk dijadikan edible film.Kata kunci: Bruguiera gymnorrhiza, edible film, kuat tarik, pati, persen pemanjangan
KARAKTERISASI NANO KITOSAN CANGKANG UDANG VANNAMEI (Litopenaeus vannamei) DENGAN METODE GELASI IONIK Pipih Suptijah; Agoes Mardiono Jacoeb; Desie Rachmania
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 14 No 2 (2011): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1736.716 KB) | DOI: 10.17844/jphpi.v14i2.5315

Abstract

Shrimp shells have a potential as raw materials for manufacturing of nanochitosan. The purposes of this study to obtain the highest yield of chitosan treated by HCl immersion time, determine the best ionic gelation process with various treatments sizing, to determine the characteristics of  nanoparticles e.g morphology, efficiency, and size of nanoparticles, analyze the characteristics of chitosan particles carried by the ionic gelation method using Fourier Transform InfraRed (FTIR) and Scanning Electron Microscopy (SEM), determine a simple method for making chitosan, that be applied easily in the laboratory. The highest yield of chitosan from shrimp shell obtained by treatment with 1 N HCl soaking time for 72 hours. Magnetic stirrer treatment could obtain high yield of nano chitosan with particle size 400-450 nm. Magnetic Stirrer could distribute the particle size homogeneously and stable than using of ultrasonic and high speed homogenizer. Degree of deacetylation of chitosan nanoparticles was 99%. Keyword: chitosan nano, FTIR, ionic gelation, SEM, white shrimp shells