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Profil Imunoglobulin-G Serum Kambing Peranakan Etawah Bunting yang Diberi Imbuhan Pakan Mineral Seng (SERUM IMUNOGLOBULIN-G LEVEL ON PREGNANT ETTAWAH CROSSBRED WERE GIVEN ZINC MINERAL) Sus Derthi Widhyari; Anita Esfandiari; I Ketut Sutama; Setyo Widodo; I Wayan Teguh Wibawan; Rizal Rahadian Ramdhany
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.664 KB) | DOI: 10.19087/jveteriner.2017.18.1.24

Abstract

The objective of this study was to find out the effect of zinc supplementation on the profile of immunoglobulin-G (IgG) in the pregnant etawah-cross ewes. Fifteen etawah-cross ewes (3-6 years old and 30-50 kg body weight) were used in this experiment and they were divided into three groups each of which consisted of 5 ewes. The first group (Zn40) received 40 ppm Zn as control, the second group (Zn60) received 60 ppm Zn, and the third group (Zn80) received 80 ppm Zn. Blood samples were collected from jugular vein every two weeks, started at twelve weeks of pregnancy up to eight weeks post partum for immunoglobulin-G analysis. IgG level were analyzed by competitive Enzyme Linked Immunosorbent Assay (ELISA) technique. Results showed that there were no significant difference of immunoglobulin-G level (P>0,05) among the three treatment groups ( Zn40, Zn60, and Zn80). However, IgG level of Zn40 and Zn80 groups tended to decrease during the last staget of pregnancy until two weeks post partum. In conclusion, supplementation of 60 ppm Zn showed higher IgG level than Zn 80 ppm. ABSTRAK Penelitian ini bertujuan untuk mengetahui efek suplementasi seng (Zn) terhadap imunoglobulin-G (IgG) pada kambing peranakan etawah (PE) bunting. Penelitian ini menggunakan 15 ekor kambing PE umur sekitar tiga sampai enam tahun, bobot badan sekitar 30-50 kg dibagi ke dalam tiga kelompok, masing-masing terdiri dari lima ekor. Kelompok Zn40 (kontrol) diberi pakan mengandung mineral Zn 40 ppm, kelompok Zn60 mengandung mineral Zn 60 ppm, dan kelompok Zn80 mengandung mineral Zn 80 ppm. Sampel darah diambil melalui vena jugularis untuk menganalisis konsentrasi IgG. Pengambilan sampel darah dilakukan setiap dua minggu mulai umur kebuntingan 12 minggu sampai delapan minggu setelah melahirkan. Hasil penelitian menunjukkan bahwa konsentrasi IgG serum tidak menunjukkan perbedaan secara nyata antar kelompok (P>0,05) antara kelompok Zn 0, Zn 40 dan Zn 80 ppm. Kelompok Zn 40 ppm dan 80 ppm cendrung memperlihatkan konsentrasi IgG mengalami penurunan pada akhir kebuntingan sampai dua minggu setelah melahirkan. Pada kelompok Zn 60 ppm memperlihatkan konsentrasi IgG lebih tinggi dibanding kelompok Zn 80 ppm.
Profil protein total, albumin dan globulin pada ayam broiler yang diberi kungiy, bawang putih dan Zinc (Zn) Sus Derthi Widhyari; Anita Esfandiari; . Herlina
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 3 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The objective of this experiment was to study the effectiveness of turmeric, garlic and zinc supplementation on protein, albumin and globulin concentration of broiler. One hundred DOC were divided into five treatments, four replications, consist of five chicks in each replicate. The treatments were RO (basal diet as a control), R1 (RO + 1,5% turmeric powder +2,5 % garlic powder), R2 (RO + 2,5% garlic powder + 120 ppm zinc), R3 (RO +1,5% turmeric powder+ 120ppm zinc) and R4 (RO +1,5 turmeric powder+ 2,5% garlic powder + 120 ppm zinc). The diet contain 23,5% crude protein and 3215 kcal metabolizable energy. Blood samples were taken from axillary veins at the three and six weeks of age. The results showed that total protein and globulin concentration at 6 weeks slightly higher than 3 weeks old chicks but not significantly different (P>0.05). Albumin concentration were highest on R3 treatment. Total protein and globulin concentration washighest on the R2 treatment. In conclusion, the supplementation of garlic (2.5%) and ZnO (120 ppm) showed the best combination to improve immune response in broiler.
Diare pada sapi neonatus yang ditantang Escherichia coli K-99 Anita Esfandiari; Sus Derthi Widhyari; Ali Hujarat
Jurnal Ilmu Pertanian Indonesia Vol. 16 No. 3 (2011): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The objective of this experiment was to study the efficacy- of colostrum given to neonatal calves challenged by Escherichia coli (E. col!) K-99. Ten healthy calves devided into two groups i.e. colostrum group (given colostrum originated from cows vaccinated by E. coli) and non-colostrum group (given whole cow milk). Colostrum or milk were given to the calves at amounts of 10% of body weight directly after birth and Followed by the same amount every 12 h, for three days. Challenged were done orally to all newborn calves when they were 12 hours of ages, using live bacteria of E. coli K-99. Fecal samples were collected every 12 hours for one week. Results of the experiment indicated that all calves experienced diarrhea following challenge tests. However, the non colostrum group showed a frequent defecation, more liquid faeces, a longer length of diarrhea and more severe clinical signs of diarrhea. It was confirmed that E' coli found and every feacal Samples,collected. In conclusion, colostrums collected from cow vaccinated by E. coli showed protective properties against E. coli K-99 infection.
The Role and Deficiency of Zinc Mineral in Immune System Sus Derthi Widhyari
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 22, No 3 (2012): SEPTEMBER 2012
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (225.953 KB) | DOI: 10.14334/wartazoa.v22i3.848

Abstract

Zinc (Zn) is a micro mineral which is needed by every cell in the body. Adequate Zn mineral is important in maintaining optimal health. Zn functions as a cofactor of various enzymes, structural integrity of cells, DNA synthesis, hormonal storage and release,  immunotransmision  and  immune  system.  Zn  deficiency causes  decreased  appetite,  dermatitis,  growth  retardation, delayed sexual maturation, infertility and imunodeficiency. This is associated with changes in the function of immune system response, such as decreased B and T cell functions, reduced phagocytosis and decreased cytokine production. Severe Zn deficiency is characterized by severely depressed immune function and frequent infections. Zn enhances immune function for specific and non-specific immunity. The role of non-specific immune respone is through the activity of cells phagocytosis by neutrophils and monocytes, while the specific immune response can be humoral and cellular mediated by lymphocytes B and T. Supplementation of Zn can improve the activity of leukocyte cells through stimulating the production of tumor necrosis factor- alpha (TNF-α) by monocytes cells, thereby increasing the ability of phagocytosis. In addition, Zn is also able to increase the production of lymphokines that cause lymphocyte cells able to differentiate and proliferate.   Key words: Zn, deficiency, immunity, leucocyte cells
Tinjauan Penambahan Mineral Zn dalam Pakan Terhadap Kualitas Spermatozoa pada Sapi Frisian holstein Jantan Sus Derthi Widhyari; Anita Esfandiari; Agus Wijaya; Retno Wulansari; Setyo Widodo; Leni Maylina
Jurnal Ilmu Pertanian Indonesia Vol. 20 No. 1 (2015): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Bulls are expected to be able to produce a good quality and quantity of sperm. The objective of this experiment was to study the effect of Zn supplementation on the sperm quality, in Frisian holstein bulls. Ten bulls, 1618 months of age were used in this experiment. The experimental bulls were divided into two groups, i.e.,group without Zn supplementation (control) and group with 60 ppm of Zn supplementation. Zn supplementations were given everyday for a period of four months. Semen was collected by using artificial vaginaat the end of the experiment. Semen quality was evaluated macroscopically and microscopically. The variables measured were semen volume, semen pH, sperm motility, sperm concentration, sperm viability, and sperm abnormality. The results showed that Zn supplementation significantly increased sperm motility and sperm concentration (P<0.05), whereas there was no significant difference in other parameters.
Profil Kadar Kortisol dan Seng pada Kambing Peranakan Etawah Saat Melahirkan yang Diberi Tambahan Seng dalam Pakannya Sus Derthi Widhyari; Setyo Widodo; I Wayan Teguh Wibawan; I Ketut Sutama; Anita Esfandiari
Jurnal Veteriner Vol 12, No 3 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective at this experiment was to study the role of zinc supplementation (Zn) in minimizingstress at parturition. Thirty etawah cross breed age 3-6 years with average body weight 30-50 kg were usedin this study and divided into three groups ; i) animal were given grass + feed concentrate + ZnSO4 40 mg/kg dry matter ( control group) ; (ii) grass + feed concentrate + ZnSO4 60 mg/kg dry matter (treatment 1), and(iii) grass + feed concentrate + ZnSO4 80 mg/kg dry matter (treatment 2), respectively drinking water weregiven ad libitum. To monitor zinc and cortisone level, blood samples were withdrawn from each animalevery two weeks starting at age of pregnancy 3 months up to two months post parturition, in addition, tomonitor the cortisone level blood samples were also collected at 1,2,3 and 7 days post parturition. The Znand cortisone serum were analyzed using atomic absorbent spectro photometric (AAS) andradioimmunoassay (RIA), respectively the result showed that generally pregnancy at the beginning andget serum level at cortisone was relatively similar between the three animal groups and there was atendency to increase and reached peak at parts then gradually decrease until the and of the observation. Atparturition the lowest serum level at cortisone (19.00±18.72 mg/ml) was observed in animals receiving the60/ dry matter zinc supplement compared to animal receiving 80 mg/ dry matter zinc ( 52.65 ± 30.83 mg/ml) and control animal ( 75.92 ± 42.88 mg/ml). in addition serum level at Zn was significantly higher inanimal receiving 60 mg and 80 mg Zn in their diets compared to control animals. The best profiles wereseen in animal given 60 mg/kg dry matter Zn supplement. In conclusion, the addition of Zn in the diets hadsignificant effect in minimizing stress as pictured by the low serum level at cortisone at parturition.
Kombinasi Imbuhan Herbal Kunyit dan Zink dalam Pakan sebagai Alternatif Pengobatan Kolibasilosis pada Ayam Pedaging (THE COMBINATION OF CURCUMIN WITH ZINC IN FEED AS ALTERNATIF THERAPY COLLIBACIILOSIS IN BROILER ) Ietje Wientarsih; Sus Derthi Widhyari; Tika Aryanti
Jurnal Veteriner Vol 14 No 3 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The use of antibiotics is one way to reduce the number of deaths in poultry industry. However, use maybe reduced because the impact for consumers. This study aims to find out the effect of the combinationbetween herbal turmeric (Curcuma domestica Val.), Garlic (Allium sativum Linn.) with Zn in the feed tothe number of erythrocyte, hematocrit and hemoglobin in chickens that is infected with Escherichia colibactery. Two hundred head of DOC were divided into 5 treatments and 4 repetitions, each repetitionconsisted of 10 chickens. Ration treatment K0 (basal ration as a healthy chickens), K (negative control)(basal ration chickens infected with Escherichia coli), P1 (basal ration + 1.5% turmeric powder + 180 ppmof ZnO / chickens infected with Escherichia coli), P2 (basal ration + 2.5% garlic powder + 180 ppm of ZnO /chickens infected with Escherichia coli), and K (positive control) (a basal ration + antibiotic / chickensinfected with Escherichia coli). At the age of 3 weeks, all treatment groups were challenged with E. coliwhich treated  orally at a dose of 108 CFU/mL. K(+) group was given antibiotics a day after the challenge. Antibiotics are given for 3 days with a dose of medication. Blood sampling performed at the age of 3 weeks(before the challenge), 1 and 2 weeks after the challenge. The blood test includes the number of erythrocytes,hematocrit values and level of hemoglobin  . The results showed the number of erythrocytes, hematocritvalues, and level of hemoglobin at treatment P1 (ration basal + 1.5% turmeric powder + 180 ppm of ZnO+ infected with Escherichia coli) tend to be higher than other treatments and have equal outcomes withtreatment K(+) (ration basal + antibiotics + infected with Escherichia coli). From the results of this studyconcluded that the use of combinations of herbs turmeric and Zn in the feed can be used as an alternativeof colibacillosis treatment.
Konsentrasi Protein Total, Albumin, dan Globulin Anak Kambing Peranakan Etawah Setelah Pemberian Berbagai Sediaan Kolostrum* (TOTAL PROTEIN, ALBUMIN, AND GLOBULIN CONCENTRATIONS ON ETTAWAH CROSSBREED NEONATES FOLLOWING THE ADMINISTRATION OF VARIOUS FORM O Anita Esfandiari; Sus Derthi Widhyari; Setyo Widodo; I Wayan Teguh Wibawan; Dondin Sajuthi; I Ketut Sutama
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This experiment was conducted to study the profile of total protein, albumin, and globulin concentrationson Ettawah crossbreed neonates after consuming various colostrums. Twenty four healthy neonatal kidswere used in this study. The neonates were divided into four groups. Each group received fresh maternal(goat) colostrum, frozen-thawed bovine colostrum, bovine spray dried colostrum, and bovine powdercommercial colostrum, respectively. Colostrums were given at 10% of body weight directly after birth andfollowed by the same amount every 12 hours, for three days. The blood was taken from jugular vein at 0, 12,24, 48, 72, and 168 hours after birth to determine total protein, albumin, and globulin concentrations.Results of this study indicated that the serum total protein and globulin concentration increased andreached the peak at 24 hours after birth. Compared to the concentration at birth, the increase of totalprotein concentration were 62.77%, 59.26%, 48.05%, and 66.67% in fresh maternal (goat), frozen-thawedbovine, bovine spray dried, and commercial bovine colostrum, respectively. Serum globulin concentrationincreased 4.9, 4.4, 4.8, and 14.6 times in fresh matermnal goat, frozen-thawed bovine, spray dried, andcommercial bovine colostrums respectively, compared to the concentration at birth. In conclusion, theconsumption of various colostrums i.e. fresh maternal goat colostrums, bovine colostrums (frozen-thawed,spray dried and commercial colostrums) would increase the concentration of blood total protein and globulin,which both reached the highest concentration at 24 h after birth.
Respons Antibodi Anti ETEC K99 pada Induk Sapi Bunting Setelah Pemberian Vaksin Escherichia Coli Polivalen Anita Esfandiari; Sus Derthi Widhyari; Sri Murtini; Bayu Febram; Retno Wulansari; Leni Maylina
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 2 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The objective of this experiment was to detect antibody (IgG) against enterotoxigenic Escherichia coli (ETEC)K99 in the blood of cows vaccinated by Escherichia coli polyvalent vaccine. Eight dry cows were injectedsubcutaneously by polyvalent Escherichia coli twice prior to parturition. Before vaccinated, the cows were givenimmunomodulator orally for 3 days. Blood samples were drawn from coccigeal vein prior to the 1st vaccination,two week following the 1st vaccination and at 1, 2, and 4 weeks after the 2nd vaccination. Blood samples wereanalyzed for IgG and ETEC K99 using indirect Enzyme Linked Immunosorbent Assay (ELISA) assay. Results of theexperiment indicated that absorbance values of all vaccinated cows before the first vaccination until third weekfollowing the 2nd vaccination were below cut off values. The absorbance values then increased and were above cutoff values at fourth week following the 2nd vaccination. In conclusion, antibody against ETEC K99, were detected inthe blood of cows, fourth week following the 2nd vaccination.
Efek Penambahan Mineral Zn Terhadap Gambaran Hematologi pada Anak Sapi Frisian Holstein Sus Derthi Widhyari; Anita Esfandiari; Agus Wijaya; Retno Wulansari; Setyo Widodo; Leni Maylina
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 3 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The objective of this experiment was to determine the effects of zinc supplementation on health status in dairy calves. Nine Frisian Holstein (FH) at 6-10 months of age were used in this experiment and divided into three groups. First group (no added Zn) for control, the second group was added 60 ppm Zn, and the third group was added 120 ppm Zn. Zn was administered daily for three months. Blood samples were collected from the jugular vein and anticoagulated with EDTA. Whole blood were used for measuring erythrocytes, hemoglobin concentration, hematocrit value, total leukocyte count, and leukocyte cell types. The results showed that no difference among groups for hematological parameters and the value of hematology were in the range values references. In conclusion, 60 and 120 ppm Zn supplementation in the feed is relatively safe for health.