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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia
Rizsa Mustika Pertiwi
Departemen Teknologi Hasil Perairan, FPIK IPB University
Agriculture, Biological Sciences & Forestry

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Perbandingan Metode Isolasi DNA pada Produk Perikanan Segar dan Olahan: Comparison of DNA Isolation Methods for Fresh and Processed Seafood Aninditya Artina Setiaputri; Giri Rohmad Barokah; Muh. Alsere Bardian Sahaba; Rahma Dini Arbajayanti; Nadia Fabella; Rizsa Mustika Pertiwi; Mala Nurilmala; Roni Nugraha; Asadatun Abdullah
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 23 No 3 (2020): Jurnal Pengolahan Hasil Perikanan Indonesia 23(3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v23i3.32314

Abstract

Seafood product is an important commodity in the global trade market, however, seafood fraud has been increasingly reported. Therefore, a method to specifically identify species of seafood products is needed. DNA methods gain many interests for detection of seafood fraud due to its robustness. This study was aimed to determine the effectiveness of several methods for isolation of DNA from fresh and processed seafood. The DNA isolation methods used in this study were the CTAB DNAzol, TianGen, Qiagen, and Fasmac.. DNA concentration and purity were determinedusing nanodrop spectrofotometry and electrophoresis. DNA isolation showed that the DNA concentration of fresh seafood products was higher than the processed seafood products. DNA isolation using CTAB method have higher levels of DNA purity (1.60-2.00) than using commercial kits in all samples. The commercial kits offer a simple procedure and minimize the possibility of environmental contaminants.
Profil Protein Ikan Gabus (Channa striata), Toman (Channa micropeltes), dan Betutu (Oxyeleotris marmorata): Protein Profile of Channa striata , Channa micropeltes, and Oxyeleotris marmorata Fish Mala Nurilmala; Mega Safithri; Fitria Tika Pradita; Rizsa Mustika Pertiwi
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 23 No 3 (2020): Jurnal Pengolahan Hasil Perikanan Indonesia 23(3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v23i3.33924

Abstract

Channa striata atau yang dikenal dengan ikan gabus adalah ikan air tawar yang memiliki banyak manfaat untuk kesehatan, khususnya kandungan albuminnya untuk penyembuhan luka. Jenis ikan air tawar lain yang diduga memiliki potensi albumin selain ikan gabus adalah ikan toman (Channa micropeltes) dan ikan betutu (Oxyeleotris marmorata). Penelitian ini bertujuan menentukan profil protein albumin pada ikan gabus, toman, dan betutu. Metode yang dilakukan yaitu ekstraksi protein, pengukuran kadar protein serta identifikasi profil protein dengan SDS-PAGE. Pengukuran kadar protein dilakukan pada ekstrak ikan segar, rebus, dan setelah pemurnian dengan presipitasi amonium sulfat. Kadar protein pada ekstrak ikan gabus segar 11,62±0,17 mg/g, rebus 6,28±0,57 mg/g dan murni 105,23±0,44 mg/g. Kadar protein ekstrak ikan toman segar 10,47±0,17 mg/g, rebus 8,19±0,33 mg/g dan murni 42,76±1,65 mg/g. Kadar protein ekstrak ikan betutu segar 4,47±0,33mg/g, rebus 2,95±0,17 mg/g dan murni 54,09±0,16 mg/g. Profil protein albumin pada ikan gabus, toman dan betutu memiliki bobot molekul 50-52 kDa.
Co-Authors Aninditya Artina Setiaputri Asadatun Abdullah Fitria Tika Pradita Giri Rohmad Barokah Mala Nurilmala MEGA SAFITHRI Muh. Alsere Bardian Sahaba Nadia Fabella Rahma Dini Arbajayanti Roni Nugraha