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Potency of Catfish (Clarias sp.) Protein Hydrolysates as Candidates Matrices for Microbiology Reference Material Kurniawati, Eti; Ibrahim, Bustami; Desniar, Desniar
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 3 (2019): December 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (579.542 KB) | DOI: 10.15578/squalen.v14i3.404

Abstract

Fish protein hydrolysate (FPH) is a derivative product of fish proteins containing smaller peptides and amino acids. FPH products have high water solubility, good emulsion capacity, and large expanding ability. With its functional properties, it allows FPH to be used as a raw material in the manufacturing of secondary microbiological reference materials. This study was intended to characterize catfish (Clarias sp.) FPH as a candidate for the matrix of microbial secondary reference. The FPH was prepared through enzymatic hydrolysis, freeze-drying and milling. The hydrolysis processes were carried out using 5% (w/w) papain, 55 °C for 5 hours, then the papain activity was stopped by increasing the temperature to 80 °C for 20 minutes.The FPH was combined with gelatine, sodium glutamate, glucose solution, and was spiked with Salmonella enteritica sv Enteritidis and freeze-dried. Results showed that catfish FPH was yellowish-white powder with a FPH yield of 11.05%. The proximate analysis of FPH revealed the moisture content of 3.77 ± 0.12%, ash content of 7.26 ± 0.03%, protein content of 86.09 ± 0.17%, and fat content of 1.38 ± 0.07%. The protein content of the FPH was greater than skim milk (33.42%). Carbohydrate levels of catfish FPH and skim milk were 1.56% and 57.46%, respectively. The best concentration of catfish FPH to perform as a microbiological reference material was 14%, obtained from highest viability of Salmonella bacteria and homogeny. The candidate for reference material were stable at storage temperatures of -20 oC.
Fermentasi Tambelo (Bactronophorus sp.) dan Karakteristik Produknya Anwar, Lely Okmawaty; Hardjito, Linawati; Desniar, Desniar
PROSIDING SEMINAR NASIONAL AGRIBISNIS PROSIDING SEMINAR NASIONAL EKONOMI MARITIM (Pengelolaan Ekonomi Maritim yang Mandiri dan Berkelanjut
Publisher : Jurusan Agribisnis Fakultas Pertanian Universitas Halu Oleo Kendari Sulawesi Tenggara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37149/17242

Abstract

Tambelo (Bactronophorus sp.) adalah hewan penggerek kayu yang dikelompokkan ke dalam filum moluska, hidup pada batang kayu bakau yang telah mati dan mengalami proses pembusukan. Pengalaman empiris masyarakat pantai Sulawesi Tenggara dan beberapa hasil penelitian menunjukkan tambelo memiliki nilai gizi yang tinggi sehingga bermanfaat bagi kesehatan. Tambelo sangat mudah mengalami pembusukan dan pengolahan tambelo belum banyak dilakukan sehingga tingkat konsumsinya rendah, oleh karena itu pembuatan tambelo fermentasi adalah cara yang tepat. Tujuan penelitian ini adalah membuat tambelo fermentasi menggunakan bakasang sebagai starter dan menentukan mutu produk akhirnya. Selama fermentasi tambelo, dilakukan analisis pH, NaCl, total bakteri, dan total bakteri asam laktat (BAL) setiap minggu selama 4 minggu, kemudian mutu produk akhir dianalisis komposisi kimia dan asam aminonya. Selama fermentasi nilai pH dan kadar NaCl mengalami penurunan, total bakteri dan total bakteri asam laktat (BAL) mengalami peningkatan sampai minggu ke dua lalu mengalami penurunan sampai minggu ke empat. Tambelo fermentasi memiliki kadar protein total yang lebih tinggi dibandingkan dengan tambelo segar dan proses fermentasi berlangsung sempurna meskipun kadarnya lebih rendah dibandingkan dengan komposisi asam amino total pada tambelo segar.
Aktivitas Antimikroba Kapang Endofit Dari Tumbuhan Pesisir Sarang Semut (Hydnophytum Formicarum) Hasil Kultivasi Nurzakiah, Nurzakiah; Desniar, Desniar; Tarman, Kustiariyah
Barakuda 45: Jurnal Ilmu Perikanan dan Kelautan Vol 2 No 1 (2020): Edisi April
Publisher : Fakultas Perikanan dan Ilmu Kelautan Universitas 17 Agustus 1945 Cirebon

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47685/barakuda45.v2i1.59

Abstract

Seven isolates of endophytic fungi from sarang semut plant (Hydnophytum formicarum) was observed for their antibacterial activity. This study aimed to determine the culture period of sarang semut endophytic fungi that has the best antibacterial activity. Screening of endophytic fungi resulted that RS1A isolate showed the widest zone of inhibition, which was 4.7 mm against Escherichia coli and Bacillus subtilis. The highest antibacterial activity of RS1A was shown by the extracts from static culture harvested on day 21. Zone of inhibition against Escherichia coli was 10 mm at a concentration of 2 mg/well and 7 mm at a concentration of 1 mg/well, while zone of inhibition against Bacillus subtilis was 7 mm and 6 mm, respectively.
Aktivitas Inhibitor Protease dari Ekstrak Karang Lunak, Asal Perairan Pulau Panggang Kepulauan Seribu Tati Nurhayati; Muhammad Fikri; Desniar Desniar
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 15, No 2 (2010): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1308.369 KB) | DOI: 10.14710/ik.ijms.15.2.59-65

Abstract

Beberapa komponen bioaktif dihasilkan oleh karang lunak, salah satunya inhibitor protease. Tujuan penelitian ini adalah mendapatkan karang lunak yang berpotensi sebagai penghambat aktivitas kerja enzim protease (inhibitor protease) pada beberapa bakteri patogen penghasil enzim protease serta mengetahui Minimum Inhibitory Concentration (MIC) dari ekstrak karang lunak tersebut.  Hasil penelitian menunjukkan bahwa pelarut metanol  lebih  potensial  untuk  mengekstrak  inhibitor  protease  dari  karang  lunak.  Karang  lunak  jenis Sarcophyton sp. dan Sinularia sp. mampu menghambat 100% aktivitas protease bakteri Staphylococus aureus dengan MIC 0,04% lebih kecil dari pada MIC EDTA (0,16%), sedangkan Xenia sp. menghambat protease bakteri S. aureus dengan MIC 0,08%. Karang lunak Nephthea sp. menghambat protease bakteri Pseudomonas aeruginosa dengan MIC 0,28%. Kata kunci : inhibitor protease, karang lunak, MIC Several bioactive compounds were produced by soft corals, including protease inhibitor.The aim of this study was to obtain softcorals which potency as inhibitor toward protease enzyme activity on pathogenic bacterial that produced protease enzyme and to study Minimum Inhibitory Concentration (MIC) from the softcorals. This research shown that ethanol is more potential for extracting protease inhibitor from softcorals. Sarcophyton sp. and Sinularia sp. are capable of inhibiting protease enzyme activity against Staphylococus aureus as 100% by MIC 0.04%, while that EDTA had MIC toward the protease as 0.16%.  Xenia sp. was capable of inhibiting protease from S. aureus by MIC 0.08%.  In the otherhand Nephtea sp. inhibited protease from Pseudomonas aeruginosa by MIC 0.28%. Key words: protease inhibitor, soft coral, MIC.