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PENGARUH EKSTRAK BIJI PEPAYA (carica papaya L.) DAN EKSTRAK DAUN MIMBA (Azadirachta indica A.Juss) DAN CAMPURAN EKSTRAK BIJI PEPAYA ( carica papaya L.) DAN EKSTRAK DAUN MIMBA (Azadirachta indica A.Juss) TERHADAP DIAMETER TUBULUS SEMINIFERUS, SEL LEYDIG DAN BOBOT TESTIS MENCIT (Mus musculus) Apolonia M; Sukarjati sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 01 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no1.a634

Abstract

Papaya (Carica papaya L. ) and Neem (Azadirachia indica A.Juss) Tree are planted as an ornamental tree and have a character as a brush plant. They vegetated in tropical and subtropical region. The seed of papaya and Neem leaf are also include of a potential plants as a antifertility. Types of bioactif compounds contained in plants, the main compounds that are derived from the steroids, alkaloids, isoflavanoid, tripernoid, and activity as xanthon antifertilities. This encourages the researcher to know the influence of ethanol seed papaya extract and neem leaf extract towards diameter of seminiferous tubule, leydig cell and the testicular weight mice (Mus musculus). The sample of this research is the mice by as much as 21 tail with a weight of 20-30 grams, aged 2.5 months. Murine in to 3 groups, each group is divided 7 treatment. As for the treatments given are extracts of flowers, leaves, and roots of hibiscus with dose control, 50, and 100 mg/kg. Granting of extracts for 35 days. On day 37 mice are in surgery for testicular weight to observations taken, the volume of the testes. The testes then made preparations and measured diameter of seminiferous tubules. This research is experimental research using Random Complete Group. The data obtained were analyzed with variant analysis (ANOVA) in one direction and continued with test LSD (Least Significant Different). The results of the research there shows the influence of the ethanol papaya seed extract, meem leaf extracy and also the micture of papaya seed extract and neem leaf extract towards the weight of mice, leydig cell and the diameter of seminiferous tubule (P < 0.05). The best result to decrease the testicular weight and diameter of seminiferous tubule was showed by the neem with the weight of 100 mg/kg BB. The best result to decrease the leydig cell was showed on the mixture of papaya seed extract and neem leaf extract in dosage of 100 mg/kg BB. This study showed that papaya seed extract, neem leaf extract and the mixture of papaya seed extract and neem leaf extract able to decrease the testicular weight, diameter of seminiferous and leydig cell. In the next day, this research supposed to be able as an antifertility in male.
PENGARUH PEMBERIAN EKSTRAK BIJI PEPAYA (Carica papaya L.) , EKSTRAK DAUN MIMBA (Azadiracta indica A. Juss ) SERTA CAMPURAN EKSTRAK BIJI PEPAYADAN EKSTRAK DAUN MIMBA TERHADAP GAMBARAN HISTOLOGI GINJAL DAN HATI MENCIT JANTAN (Mus musculus L) Ervina Ervina; Sukarjati Sukarjati
WAHANA Vol 68 No 1 (2017)
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.646 KB) | DOI: 10.36456/wahana.v68i1.629

Abstract

Penelitian ini bertujuan untuk membuktikan pengaruh ekstrak biji pepaya (Caricapapaya L), ekstrak daun mimba (Azadiracta indica A, Juss) serta campuran ekstrak biji pepaya dan daun mimba terhadap gambaran histologi ginjal dan hati mencit jantan (Mus musculus L).Sejumlah 21 ekor menit dibagi menjadi 7 kelompok. Kelompok kontrol diberi aqudest secara per oral, kelompok ekstrak biji pepaya (dosis 50, 100 mg/kg bb), kelompok ekstrak daun mimba (dosis 50, 100 mg.kg bb) dan kelompok campuran ekstrak biji pepaya danekstrakdaunmimba (dosis 50,100 mg/kg bb). Pada hari ke 36 dilakukan pembedahan dan dilakukan pemeriksaan histopatologi pada organ ginjal dan hati. Hasil penelitian menunjukkan adanya kerusakan histologi hati berupa oedema, kongesti, hemoragi, degenerasi, sedangkan pada ginjal menuunjukkan adanya kerusakan yaitu degenerasi tubulus proksimal, degenerasi tubulus distal, dan perdarahan interstinal. Kesimpulan dari penelitian ini bahwa ekstrak biji pepaya, ekstrak daun mimba, dan campuran ekstrak biji pepaya dan daun mimba memberikan pengaruh significan (p
KERUPUK SAMILER FORTIFIKASI KALSIUM DARI DURI IKAN BANDENG Sukarjati Sukarjati; Sulistyowati Sulistyowati
E-Dimas: Jurnal Pengabdian kepada Masyarakat Vol 6, No 2 (2015): E-DIMAS
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/e-dimas.v6i2.952

Abstract

Kerupuk Samiler cukup dihargai oleh nilai gizi masyarakat rendah karena hanya terbuat dari singkong. Proses produksi tidak higienis karena dicetak menggunakan bak plastik warna warni. Pengeringan hanya mengandalkan sinar matahari. Hal ini menyebabkan tidak ada penghasilan tetap. Pemasaran terbatas pada daerah sekitar produksi dan disimpan di toko atau dijual keliling. Tujuan dari program IbM ini adalah untuk mengatasi masalah tersebut. Metode yang digunakan adalah konseling, pelatihan, dan mentoring dalam produksi kerupuk samiler asli dan kerupuk samiler fortifikasi duri ikan yang higienis dan melakukan pelatihan dan mentoring manajemen produksi dan manajemen pemasaran. Hasil dari program ini adalah berdasarkan uji kadar kalsium dalam adonan kerupuk samiler yang diberi duri ikan setinggi 51.28 mg/100g dibandingkan dengan adonan kerupuk yang tidak diberi duri ikan yaitu 9.88 mg/100 g. Keterampilan dan pengetahuan pembuat kerupuk samiler tentang manajemen produksi dan manajemen pemasaran meningkat. Telah diproduksi kerupuk samiler asli dan fortifikasi kalsium duri bandeng. Kedua produk ini diproses dan dikemas secara higienis. Pengeringan kerupuk di musim hujan dapat diatasi menggunakan mesin pengering kerupuk dengan bahan bakar LPG. Kesimpulan dari program IbM adalah pembuat kerupuk samiler meningkatkan pendapatan dan penghasilan tetap. ?é?á Kata Kunci: Kerupuk Samiler, Fortifikasi Kalsium, Duri Bandeng
DETEKSI DELESI GEN DAZ (Deleted in AZoospermia) PADA PRIA AZOOSPERMIA DENGAN METODE PCR (Polymerase Chain Reaction) V.A. Ferandra; Sukarjati Sukarjati
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 1 No 1 (2017): Medical Technology and Public Health Journal March 2017
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v1i1.759

Abstract

At this time the case of azoospermia is quite common in infertile men. Azoospermia is a condition where the semen does not contain sperm. Many causes azoospermia, including the deletion of a gene at the locus that is located on the Y chromosome long arm (YQ) known as AZF gene (Azoospermia Factor). One of the genes in the AZF region are genes that AZFc DAZ (Deleted in Azoospermia). The purpose of this study was to detect the presence of the DAZ gene deletions in men with azoospermia cases using PCR (Polymerase Chain Reaction). The study design was descriptive. Venous blood samples with EDTA anticoagulant taken from 10 men azoospeermia then extracted to obtain DNA. DNA samples were then carried out PCR with primers DAZ. The PCR products were separated by electrophoresis in 2% agarose gel and visualized using UVtranslluminator. Of the 10 samples, four patients including DAZ gene deletion was detected experience while the other six do not experience DAZ gene deletions. It concluded that found their DAZ gene deletions in men with azoospermia using the PCR method
TEKNOLOGI SISTEM INTEGRASI TERNAK, TANAMAN, DAN IKAN PADA LAHAN PEKARANGAN DAN TEKNOLOGI PENGOLAHAN BAHAN MAKANAN GUNA PENINGKATAN PENDAPATAN KELUARGA DI DESA CANGKRINGTURI, SIDOARJO Sukarjati Sukarjati; Tatang Sopandi; Pungky Slamet WK
Jurnal Abadimas Adi Buana Vol 1 No 2 (2017): Oktober
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.114 KB) | DOI: 10.36456/abadimas.v1.i2.a948

Abstract

Objective of KKN-PPM is to overcome the problems encountered in the village Cakringturi that the low incomes, lack of knowledge and understanding of environmental management, especially land yard. The solution to solve the problem is to empower rural communities through land use courtyard with technology integration system of livestock, crops and fish and provide food processing technology skills.Methods: Counseling, Mentoring, Training. Result: K1 program: Land yard citizens have been utilized, Product Probiofish, Aquaponics, Raising catfish in the pond Bis, The Carambola Fruit Cultivation along the river, Product instant and syrup TOGA, Vertikultur. K2 program: Competition Fun Cake in early childhood, Competition Beuty and Hijab Class, Teaching in Early Childhood Educatio. K3 Program : : Tutoring for elementary school children, Work with the community service, Yasinan, tahlilan, & diba'an, Assist Posyandu services. Distribution of poor rice, Visits to polindes,, Decorating in early childhood classrooms, Coordination with Karangtaruna, A visit to the village, Assist village. Conclusion: The entire planning of the program, both the main program, additional, or auxiliary implemented very well and smoothly by all students of KKN PPM 2015. Society and village and community leaders strongly support KKN PPM program and community empowerment have been successfully carried out. Keywords : Community EmpowermentCatfish in the pond bis, Aquaponics, Vertikultur, Star fruit cultivation, Instant and Syrup Toga.
DAMPAK INFEKSI Escherichia coil DAN ADHFSIN PILI Escherichia coil ISOLAT SEMEN PRIA INFERTIL BM 32.2 KDA PADA MOTILITAS, VITALITAS DAN MORFOLOGI SPERMATOZOA MARMUT Sukarjati Sukarjati; Sudjarwo Sudjarwo
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 4 No 01 (2010)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol4.no1.a1086

Abstract

Penelitian ini bertujuan untuk mengkaji dampak infeksi E. coil dan imunisasi adhesin pill E. coil BM 32.2 kDa terhadap kualitas spermatozoa Marmut. Sampel penelitian ini. adalah spermatozoa dari 15 Marmut. Uji toksisitas adhesin pili E. coil BM 32.2 kDa terhadap kualitas spermatozoa marmut dilakukan dengan mengamati motilitas, vitalitalis dan morfologi sperms marmut imicroskop. Hasil penelitian menunjukkan bahwa tidak ada perbedaan antara marmut kontrol negatif dan marmut perlakuan (yang diimunisasi dengan adhesin pili E. coil BM 32.2 kDa ) pada motilitas spermatozoa (p0.499), vitalitas spermatozoa (p.817) dan morfologi normal spermatozoa (p--3.176). Antara marmut kontrol negatif dan marmut kontrol positif (yang diinfeksi dengan E. coli secara transuretral) ada perbedaan balk pada motilitas (p0.000), vitalitas (p=0.000) maupun morfologi (p=0.000). Antara marmut kontrol positif dan marmut perlakuan ada perbedaan pada motilitas (p-71.001), vitalitas (p=0.000) dan morfologi (p-.000). Hasil pengamatan morfologi menunjukkan bahwa pada marmut yang infeksi secara tranuretral dengan E. coil tampak adanya granulosit dan E. coil yang melekat pada sperms epididimis. Berdasarkan basil penelitian ini dapat disimpulkan bahwa infeksi E. coil secara transuretral berdampak menurunkan kualitas sperm epididimis marmut dan protein adhesin pili E. coil BM 32.2 kDa yang diimtmisasikan ke marmut tidak berpengaruh terhadap kualitas spermatozoa epididimis Marmut.
ROLE OF IMMUNOGLOBULIN G (IgG) FROM THE INDUCTION OF Escherichia coli PILI ADHESION PROTEIN ISOLATED FROM INFERTILE MALE SEMEN WITH 32.2 KDA MOLECULAR WEIGHT AS OPSONIN AND ANTI-ADHESION AN IN VITRO Escherichia coli INFECTION Sukarjati Sukarjati; Susie Amilah
Folia Medica Indonesiana Vol. 53 No. 2 (2017): JUNE 2017
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.031 KB) | DOI: 10.20473/fmi.v53i2.6343

Abstract

Escherichia coli (E. coli), a major cause of male genital tract infections, is asymptomatic and may result in male infertility. We have succeeded in isolating and characterizing proteins of E. Pili coli isolates from semen of infertile men who function as adhesin with a molecular weight (MW) 32.2 kDa. This study aims to prove the ability of IgG results adhesion proteins induced pili of E. MW coli 32.2 kDa as opsonin to determine the value of the activity and phagocytic capacity and as an anti- adhesion by calculating the average number of E. coli that attached to human spermatozoa. E. coli infertile men's semen were cultured using standard bacteriology. Peritoneal macrophages were isolated from mice. Spermatozoa from donors were prepared using Sil with Select Plus. IgG was obtained from mice immunized with (1) PBS (control), (2) E. coli pili adhesion protein isolated from infertile men semen with MW of 32.2 kDa and (3) weakened E. coli isolated from infertile men's semen. Phagocytic activity value was determined by counting the number of cells activated macrophage phagocytosis process in 100 cells. Phagocytic capacity value was determined by counting the number of bacteria ingested by 25 macrophages. Anti-adhesion test was done by counting the number of bacteria attached to 100 spermatozoa. The results of this study showed difference (p=0.000) in phagocytic activity and phagocytic capacity (p=0.000) between treatment (1) and (2), and between treatment (1) to (3). However, treatment (2) and (3) did not differ neither in phagocytic activity (p=0.693) nor in phagocytosis capacity (p=0.125). Anti-adhesion test produces difference (p=0.000) in the number of E. coli that attached to human spermatozoa between treatments (1) and treatment (2), and between treatments (1) and (3). The number of E. coli that attached to human spermatozoa between treatment (2) and treatment (3) was not significantly different (p=0.371). In conclusion, IgG from the induction of E. coli pili adhesion protein of infertile men semen isolates with MW of 32.2 kDa can increase phagocytic activity and capacity as well as serve as an anti- adhesion. Thus, IgG from the induction of E. coli pili adhesion protein of infertile men semen isolates with MW of 32.2 kDa is protective against in vitro E. coli infection, so that it can be used as material to prevent male reproductive tract infections due to E. coli.
KONSENTRASI HAMBAT MINIMUM EKSTRAK ETHANOL DAUN BELUNTAS (Pluchea indicha) DAN DAUN KEMANGI (Ocimum sanctum L) SERTA CAMPURAN DAUN BELUNTAS DAN DAUN KEMANGI TERHADAP PERTUMBUHAN BAKTERI (Escherichia coli, Staphylococcus aureus) PADA PENDERITA PROSTATITIS Isnainy Hidayati; Sukarjati Sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1030

Abstract

Prostatitis is a disease caused by bacteria, especially (E. coli, S. aureus). Treatment of diseases using antibiotics cause negative effects on the environment, pathogenic bacterial resistance, and antibiotic residues. Therefore, it is necessary to develop an antibacterial alternative from natural ingredients for the treatment of this disease. One of the natural ingredients that are antibacterial is basil leaf extract and beluntas leaves that have antibacterial inhibitory power. This research aims to prove the antibacterial activity of basil leaf extract and beluntas leaves in vitro. The treatments used were P0 (control), P1 (1.56%), P2 (3.125%), P3 (6.25%), P4 (12.5%), P5 (25%), P6 (50% ), P7 (100% ).. The parameters observed were bacterial growth on culture medium. The result of MIC (the lowest concentration of dilution that can inhibit bacterial growth) is seen from the treatment media turbidity and MBC result (the lowest concentration of dilution that can kill bacteria) seen from bacterial colony growth on NAP media. Data were analyzed descriptively. The results showed that basil leaf extract and beluntas leaves can inhibit growth or killing (E. coli, S. aureus) in prostatitis patients. Keywords: Prostatitis, antibacterial activity, microbial inhibition
PENGARUH EKSTRAK ETANOL BUNGA, DAUN, DAN AKAR KEMBANG SEPATU (Hibiscus rosa sinensis) TERHADAP BERAT, VOLUME, DAN DIAMETER TUBULUS SEMINIFERUS MENCIT (Mus musculus) Rojib Rojib; Sukarjati Sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 9 No 01 (2016)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol9.no01.a329

Abstract

Hibiscus (Hibiscus rosa sinensis) many are grown as ornamental plants, shrubs, and grown in the tropics andsubtropics. Hibiscus is also one kind of plant that is potentially as antifertility. Types of bioactif compoundscontained in plants, the main compounds that are derived from the steroids, alkaloids, isoflavanoid,tripernoid, and activity as xanthon antifertilities. This encourages the researcher to know the influence ofethanol ekstract flowers, leaves, and roots of hibiscus (Hibiscus rosa sinensis) against the weight, volumeand diameter ofseminiferoustubules mice (Mus musculus). The sample of this research is the mice by asmuch as 27 tail with a weight of 20-30 grams, aged 2.5 months. Murine in to 3 groups, each group is divided3 treatment. As for the treatments given are extracts of flowers, leaves, and roots of hibiscus with dosecontrol, 150, and 300 mg/kg. Granting of extracts for 35 days. On day 37 mice are in surgery for testicularweight to observations taken, the volume of the testes. The testes then made preparations and measureddiameter of seminiferoustubules. This research is experimental research using Random Design Group. Thedata obtained were analyzed with variant analysis (ANOVA) in one direction and continued with test LSD(Least Significant Different). The results of the research there shows the influence of the ethanol extracts offlowers, leaves, and roots of hibiscus against testicular volume, weight, and diameter of seminferoustubules (P < 0.05). The best treatment that can decrease the weight, volume and diameterofseminiferoustubules is on the flower with a dose of 300 mg/kg. The results of this research it can beconcluded that extracts of flowers, leaves, and roots can lose weight, testicular volume and diameter ofseminiferoustubules. In future it is hoped the research could be developed as material antifertilitas in men. Keywords:Extract of flower, Laves, roots of (Hibiscus rosa sinensis), testicles, diameter of seminiferous tubules, mice (Mus musculus).
PENGARUH EKSTRAK ETIL ASETAT GETAH KAMBOJA (Plumeria Acumenate.W.T.Ait) TERHADAP PERTUMBUHAN DAN DAYA HAMBAT BAKTERI Staphylococus aureus R. T. Wahyudi; Sukarjati sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 6 No 02 (2013)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol6.no02.a525

Abstract

Frangipani sap (Plumeria acumenate.WTAit) was known to use as an antiseptic. This study aimed to prove ethyl acetate extract of frangipani sap as antibacterial Staphylococcus aureus. Frangipani sap from apical stem was extracted with chloroform and fractionated with ethyl acetate. Concentration of ethyl acetate extract (0%, 5%, 10%, 15%, 20%, and 25%) were tested against the growth of bacteria S. aureus using plate count and diffusion agar method. The results showed concentrations of frangipani sap extract significantly (P <0.05) on the growth of S. aureus. Highest inhibitions of frangipani sap extract on growth of S. aureus have obtained at a concentration of 25%. Key words: frangipani sap, ethyl acetate, S. aureus