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Journal : Stigma : Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa

PENGARUH EKSTRAK BIJI PEPAYA (carica papaya L.) DAN EKSTRAK DAUN MIMBA (Azadirachta indica A.Juss) DAN CAMPURAN EKSTRAK BIJI PEPAYA ( carica papaya L.) DAN EKSTRAK DAUN MIMBA (Azadirachta indica A.Juss) TERHADAP DIAMETER TUBULUS SEMINIFERUS, SEL LEYDIG DAN BOBOT TESTIS MENCIT (Mus musculus) Apolonia M; Sukarjati sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 01 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no1.a634

Abstract

Papaya (Carica papaya L. ) and Neem (Azadirachia indica A.Juss) Tree are planted as an ornamental tree and have a character as a brush plant. They vegetated in tropical and subtropical region. The seed of papaya and Neem leaf are also include of a potential plants as a antifertility. Types of bioactif compounds contained in plants, the main compounds that are derived from the steroids, alkaloids, isoflavanoid, tripernoid, and activity as xanthon antifertilities. This encourages the researcher to know the influence of ethanol seed papaya extract and neem leaf extract towards diameter of seminiferous tubule, leydig cell and the testicular weight mice (Mus musculus). The sample of this research is the mice by as much as 21 tail with a weight of 20-30 grams, aged 2.5 months. Murine in to 3 groups, each group is divided 7 treatment. As for the treatments given are extracts of flowers, leaves, and roots of hibiscus with dose control, 50, and 100 mg/kg. Granting of extracts for 35 days. On day 37 mice are in surgery for testicular weight to observations taken, the volume of the testes. The testes then made preparations and measured diameter of seminiferous tubules. This research is experimental research using Random Complete Group. The data obtained were analyzed with variant analysis (ANOVA) in one direction and continued with test LSD (Least Significant Different). The results of the research there shows the influence of the ethanol papaya seed extract, meem leaf extracy and also the micture of papaya seed extract and neem leaf extract towards the weight of mice, leydig cell and the diameter of seminiferous tubule (P < 0.05). The best result to decrease the testicular weight and diameter of seminiferous tubule was showed by the neem with the weight of 100 mg/kg BB. The best result to decrease the leydig cell was showed on the mixture of papaya seed extract and neem leaf extract in dosage of 100 mg/kg BB. This study showed that papaya seed extract, neem leaf extract and the mixture of papaya seed extract and neem leaf extract able to decrease the testicular weight, diameter of seminiferous and leydig cell. In the next day, this research supposed to be able as an antifertility in male.
DAMPAK INFEKSI Escherichia coil DAN ADHFSIN PILI Escherichia coil ISOLAT SEMEN PRIA INFERTIL BM 32.2 KDA PADA MOTILITAS, VITALITAS DAN MORFOLOGI SPERMATOZOA MARMUT Sukarjati Sukarjati; Sudjarwo Sudjarwo
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 4 No 01 (2010)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol4.no1.a1086

Abstract

Penelitian ini bertujuan untuk mengkaji dampak infeksi E. coil dan imunisasi adhesin pill E. coil BM 32.2 kDa terhadap kualitas spermatozoa Marmut. Sampel penelitian ini. adalah spermatozoa dari 15 Marmut. Uji toksisitas adhesin pili E. coil BM 32.2 kDa terhadap kualitas spermatozoa marmut dilakukan dengan mengamati motilitas, vitalitalis dan morfologi sperms marmut imicroskop. Hasil penelitian menunjukkan bahwa tidak ada perbedaan antara marmut kontrol negatif dan marmut perlakuan (yang diimunisasi dengan adhesin pili E. coil BM 32.2 kDa ) pada motilitas spermatozoa (p0.499), vitalitas spermatozoa (p.817) dan morfologi normal spermatozoa (p--3.176). Antara marmut kontrol negatif dan marmut kontrol positif (yang diinfeksi dengan E. coli secara transuretral) ada perbedaan balk pada motilitas (p0.000), vitalitas (p=0.000) maupun morfologi (p=0.000). Antara marmut kontrol positif dan marmut perlakuan ada perbedaan pada motilitas (p-71.001), vitalitas (p=0.000) dan morfologi (p-.000). Hasil pengamatan morfologi menunjukkan bahwa pada marmut yang infeksi secara tranuretral dengan E. coil tampak adanya granulosit dan E. coil yang melekat pada sperms epididimis. Berdasarkan basil penelitian ini dapat disimpulkan bahwa infeksi E. coil secara transuretral berdampak menurunkan kualitas sperm epididimis marmut dan protein adhesin pili E. coil BM 32.2 kDa yang diimtmisasikan ke marmut tidak berpengaruh terhadap kualitas spermatozoa epididimis Marmut.
KONSENTRASI HAMBAT MINIMUM EKSTRAK ETHANOL DAUN BELUNTAS (Pluchea indicha) DAN DAUN KEMANGI (Ocimum sanctum L) SERTA CAMPURAN DAUN BELUNTAS DAN DAUN KEMANGI TERHADAP PERTUMBUHAN BAKTERI (Escherichia coli, Staphylococcus aureus) PADA PENDERITA PROSTATITIS Isnainy Hidayati; Sukarjati Sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1030

Abstract

Prostatitis is a disease caused by bacteria, especially (E. coli, S. aureus). Treatment of diseases using antibiotics cause negative effects on the environment, pathogenic bacterial resistance, and antibiotic residues. Therefore, it is necessary to develop an antibacterial alternative from natural ingredients for the treatment of this disease. One of the natural ingredients that are antibacterial is basil leaf extract and beluntas leaves that have antibacterial inhibitory power. This research aims to prove the antibacterial activity of basil leaf extract and beluntas leaves in vitro. The treatments used were P0 (control), P1 (1.56%), P2 (3.125%), P3 (6.25%), P4 (12.5%), P5 (25%), P6 (50% ), P7 (100% ).. The parameters observed were bacterial growth on culture medium. The result of MIC (the lowest concentration of dilution that can inhibit bacterial growth) is seen from the treatment media turbidity and MBC result (the lowest concentration of dilution that can kill bacteria) seen from bacterial colony growth on NAP media. Data were analyzed descriptively. The results showed that basil leaf extract and beluntas leaves can inhibit growth or killing (E. coli, S. aureus) in prostatitis patients. Keywords: Prostatitis, antibacterial activity, microbial inhibition
PENGARUH EKSTRAK ETANOL BUNGA, DAUN, DAN AKAR KEMBANG SEPATU (Hibiscus rosa sinensis) TERHADAP BERAT, VOLUME, DAN DIAMETER TUBULUS SEMINIFERUS MENCIT (Mus musculus) Rojib Rojib; Sukarjati Sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 9 No 01 (2016)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol9.no01.a329

Abstract

Hibiscus (Hibiscus rosa sinensis) many are grown as ornamental plants, shrubs, and grown in the tropics andsubtropics. Hibiscus is also one kind of plant that is potentially as antifertility. Types of bioactif compoundscontained in plants, the main compounds that are derived from the steroids, alkaloids, isoflavanoid,tripernoid, and activity as xanthon antifertilities. This encourages the researcher to know the influence ofethanol ekstract flowers, leaves, and roots of hibiscus (Hibiscus rosa sinensis) against the weight, volumeand diameter ofseminiferoustubules mice (Mus musculus). The sample of this research is the mice by asmuch as 27 tail with a weight of 20-30 grams, aged 2.5 months. Murine in to 3 groups, each group is divided3 treatment. As for the treatments given are extracts of flowers, leaves, and roots of hibiscus with dosecontrol, 150, and 300 mg/kg. Granting of extracts for 35 days. On day 37 mice are in surgery for testicularweight to observations taken, the volume of the testes. The testes then made preparations and measureddiameter of seminiferoustubules. This research is experimental research using Random Design Group. Thedata obtained were analyzed with variant analysis (ANOVA) in one direction and continued with test LSD(Least Significant Different). The results of the research there shows the influence of the ethanol extracts offlowers, leaves, and roots of hibiscus against testicular volume, weight, and diameter of seminferoustubules (P < 0.05). The best treatment that can decrease the weight, volume and diameterofseminiferoustubules is on the flower with a dose of 300 mg/kg. The results of this research it can beconcluded that extracts of flowers, leaves, and roots can lose weight, testicular volume and diameter ofseminiferoustubules. In future it is hoped the research could be developed as material antifertilitas in men. Keywords:Extract of flower, Laves, roots of (Hibiscus rosa sinensis), testicles, diameter of seminiferous tubules, mice (Mus musculus).
PENGARUH EKSTRAK ETIL ASETAT GETAH KAMBOJA (Plumeria Acumenate.W.T.Ait) TERHADAP PERTUMBUHAN DAN DAYA HAMBAT BAKTERI Staphylococus aureus R. T. Wahyudi; Sukarjati sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 6 No 02 (2013)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol6.no02.a525

Abstract

Frangipani sap (Plumeria acumenate.WTAit) was known to use as an antiseptic. This study aimed to prove ethyl acetate extract of frangipani sap as antibacterial Staphylococcus aureus. Frangipani sap from apical stem was extracted with chloroform and fractionated with ethyl acetate. Concentration of ethyl acetate extract (0%, 5%, 10%, 15%, 20%, and 25%) were tested against the growth of bacteria S. aureus using plate count and diffusion agar method. The results showed concentrations of frangipani sap extract significantly (P <0.05) on the growth of S. aureus. Highest inhibitions of frangipani sap extract on growth of S. aureus have obtained at a concentration of 25%. Key words: frangipani sap, ethyl acetate, S. aureus
PENGARUH PEMBERIAN EKSTRAK ETANOL BUNGA, DAUN DAN AKAR KEMBANG SEPATU (Hibiscus rosa sinensis) TERHADAP HISTOLOGI TESTIS MENCIT (Muss muscullus) D. Febrianti; Sukarjati Sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 8 No 02 (2015)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol8.no02.a1581

Abstract

Kembang sepatu ( Hibiscus rosa sinensis) merupakan salah satu tanaman herbal. Pada bagian bunga memiliki kandungan flavonoid, pada bagian daun mengandung saponin dan flavonoid sedangkan pada bagian akar mengandung flavonoid, saponin dan tanin. Senyawa flavonoid, saponin, dan tanin ini merupakan senyawa yang berfungsi sebagai antifertilitas untuk kaum pria. Hal inilah yang mendorong penulis untuk melakukan penelitian mengenai pengaruh ekstrak bunga, daun, dan akar kembang sepatu (Hibiscus rosa sinensis) terhadap histologi testis mencit ( Mus muscullus). Sampel penelitian ini adalah testis mencit sebanyak 27 ekor dengan berat badan 20-30 gram, berumur 2,5 bulan. Mencit di bagi 3 kelompok, masing-masing kelompok dibagi 3 perlakuan. Adapun perlakuan yang diberikan adalah ekstrak bunga, ekstrak daun, dan ekstrak akar kembang sepatu dengan konsentrasi 0mg/kg bb, 150 mg/kg bb dan 300 mg/kg bb. Pemberian ekstrak dilakukan 35 hari lamanya. Pada hari ke 37 mencit di bedah untuk diambil cauda testisnya untuk diamati histologi testis pada mencit. Penelitian ini merupakan penelitian eksperimental dengan menggunakan Rancangan acak kelompok (RAK). Pengamatan histologi testis mencit meliputi jumlah sel spermatogonia, sel spermatosit, sel spermatid, dan sel leydig dengan menggunakan mikroskop. Data yang diperoleh dianalisis dengan analisis varian (ANOVA) Satu arah. Hasil dari penelitian ini menujukkan ada pengaruh ekstrak bunga, daun, dan akar kembang sepatu terhadap jumlah sel spermatogonia (P<0,05), jumlah sel spermatosit (P<0,05), jumlah sel spermatid (P<0,05) dan jumlah sel leydig (P<0,05). Hasil penelitian ini dapat disimpulkan bahwa ekstrak daun dengan konsentrasi 300 mg/kg bb adalah perlakuan yang optimal dalam menurunkan jumlah sel spermatogonia, jumlah sel spermatosit, jumlah sel spermatid dan jumlah sel leydig.