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Isolasi dan Identifikasi Bakteri Listeria monocytogenes dari Susu Sapi Segar di Kabupaten Enrekang Sulawesi Selatan Kusumandari Indah Prahesti; Ni Luh Putu Ika Mayasari; Ratmawati Malaka; Farida Nur Yuliati; Fachriyan Hasmi Pasaribu
Acta VETERINARIA Indonesiana Vol. 5 No. 2 (2017): Juli 2017
Publisher : IPB University

Listeria monocytogenes merupakan bakteri patogen yang dapat menginfeksi manusia melalui bahan pangan sehingga menimbulkan penyakit listeriosis. Wabah listeriosis terjadi akibat konsumsi bahan pangan yang terkontaminasi L. monocytogenes, di antaranya daging, susu, dan produk susu. Serotipe bakteri L. monocytogenes dikaitkan dengan kasus wabah epidemik dan sporadik listeriosis pada manusia. Penelitian ini bertujuan untuk mengisolasi L. monocytogenes dari susu sapi segar di Kabupaten Enrekang Sulawesi Selatan, melakukan analisis karakteristik molekuler, dan menentukan serotipe isolat bakteri L. monocytogenes yang diperoleh. Sebanyak 107 sampel susu diperoleh dari lima kecamatan di Kabupaten Enrekang dan dikumpulkan menjadi 31 sampel pool, kemudian dilakukan isolasi dan identifikasi bakteri. Tahap pengayaan dilakukan dengan media Listeria enrichment broth (LEB) kemudian dilakukan kultur pada media Listeria selective agar base (LSA), dilanjutkan dengan uji biokimiawi. Isolat bakteri L. monocytogenes yang diperoleh dikonfirmasi dengan polymerase chain reaction (PCR) dan dilakukan pengurutan oligonukleotida. Identifikasi serotipe dilakukan dengan PCR multipleks. Hasil menunjukkan bahwa sebanyak 21 isolat merupakan bakteri L. monocytogenes dan analisis pengurutan oligonukleotida menunjukkan bahwa isolat yang diperoleh memiliki kemiripan sebesar 99% dengan strain L. monocytogenes yang terdapat pada basis data GenBank. Identifikasi serotipe menunjukkan bahwa keseluruhan isolat termasuk dalam serogrup 2, yaitu serotipe 1/2c dan 3c.

Potensi Infusa Kemiri (Aleurites moluccana) sebagai Analgesik dan Stimulator Stamina Fajar Anaba; Andriyanto; Ni Luh Putu Ika Mayasari
Acta VETERINARIA Indonesiana Vol. 9 No. 1 (2021): Maret 2021
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.9.1.14-20

Kesehatan merupakan hal penting untuk menjalankan aktivitas sehari-hari. Penurunan kesehatan dan daya tahan tubuh mengakibatkan timbul rasa nyeri serta mudah terserang penyakit. Pengobatan herbal digunakan sebagai pengobatan alternatif yang lebih aman dan terjangkau dibandingkan pengobatan nonherbal dari bahan-bahan kimia. Kemiri merupakan salah satu tanaman herbal yang memiliki banyak khasiat dan sering digunakan sebagai pengobatan oleh masyarakat. Penelitian ini bertujuan mempelajari efektivitas infusa kemiri sebagai analgesik dan stimulator stamina dalam berbagai dosis pada mencit. Uji efektivitas analgesik ditinjau menggunakan metode hot water immersion tail-flick test dan uji efektivitas stamina menggunakan metode natatory enhausen. Penelitian ini menggunakan 20 ekor mencit jantan yang dikelompokkan menjadi kelompok kontrol dan kelompok perlakuan yang diberi infusa kemiri dengan dosis 1, 2, dan 4 g/kg BB. Setiap kelompok terdiri atas 5 ekor. Data dianalisis menggunakan analysis of variance (ANOVA) kemudian dilanjutkan dengan uji Tukey. Hasil penelitian didapatkan dosis efektif pada uji analgesik adalah 4 g/kg BB dengan waktu respons nyeri ekor terlama yaitu 7.840±0.477 detik dan pada uji stamina adalah 1 g/kg BB yang ditunjukkan dengan durasi berenang terlama yaitu 145.00±20.65 detik. Kemiri memiliki efektivitas terhadap analgesik dan stimulator stamina.

MP-8 Lack of Antibody Formation Against Inactivated Avian Influenza Virus in Ducks and Chickens After Intranasally Immunization Okti Nadia Poetri; Retno Damayanti Soejoedodo; Ni Luh Putu Ika Mayasari; Ekowati Handharyani; Novera Nirmalasanti
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.484 KB)

Vaccination is one of control strategies implemented in endemic countries such as Egypt and Indonesia [1,2]. Most commercial AI vaccines available in Indonesia are adjuvanted inactivated AI vaccines applied through intramuscular routes. Vaccine application by subcutaneous or intramuscular injection can cause pain and stress in poultry, the route of vaccine through the nasal drip (intranasal) is a more convenient and painless. However, respiratory applied inactivated influenza is poorly immunogenic. Therefore prior to developing inactivated intranasal vaccine, it is necessary to study antibody response to inactivated AI virus which exposed through intranasal route. The aim of our research was to determined antibody response of ducks and chickens against avian influenza virus (AIV) subtype H5N1 after intranasally immunization.

Dekontaminasi Mycobacterium avium subspecies paratuberculosis pada feses menggunakan beberapa jenis desinfektan Ika Suharti; Ni Luh Putu Ika Mayasari; Fachriyan Hasmi Pasaribu
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Paratuberculosis or Johne’s Disease is a granulomatous enteritis chronic disease of domestic and wild ruminants caused by infection of Mycobacterium avium subspecies paratuberculosis. The disease commonly infects dairy cattle with clinical signs of chronic diarrhea, decreasing body weight, low milk production, oedema, anemia and occasionally infertility. The basic procedure in order to control Paratuberculosis in farms is to do a good and proper handling of animal faecal. Disinfection of animal environments such as pens, faecal, sewerage and sewage are important in prevention of transmission of this disease. The purpose of this research is to determine specific disinfectan and dosage for Mycobacterium avium subspecies paratuberculosis decontamination in cattle feces so it can be applied as disease control measures. Cow's feces were contaminated with MAP 105CFU/ml and treated with ammonium quartener, phenolic and formaldehyde disinfectant doses 10%, 15% and 20%. The effectiveness of the disinfectant was tested based on MAP identification using Löwenstein-Jensen culture medium and nested Polymere Chain Reaction(PCR). The results showed 15% and 20% doses of formaldehyde disinfectants efective to decontaminate Mycobacterium avium subspecies paratuberculosis in catle feces.

Penggunaan Antigen Mycoplasma gallisepticum Freeze-Thawing dalam Teknik Enzyme-Linked Immunosorbent Assay Faidah Rachmawati Rachmawati; I Wayan Teguh Wibawan; Ni Luh Putu Ika Mayasari
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Chronic Respiratory Disease (CRD) is a bacterial infection in chickens caused by Mycoplasma gallisepticum. CRD may result in economic loss in the livestock industry, decreasing of egg production and feed efficiency, increasing of medication costs, downgrading of carcass and egg qualities. The aim of this study was to develop the Enzyme-Linked Immunosorbent Assay (ELISA) kit for detecting antibodies of M. gallisepticum using freeze-thawing antigens. The cut-off point was determined by S/N method. Chicken antiserum againts M. gallisepticum was collected every week on 1st – 6th week post immunization of whole cell antigen of M. gallisepticum. Optimization of antigen, serum and conjugate were 10 μg/ml protein concentrations, 1:800 serum dilution and 1:10000 conjugate dilution. The validation results on developed ELISA kit (MyGELISA) analyzed by ROC curve using MedCalc statistical software revealed that developed ELISA kit (MyGELISA) had 100% sensitivity and 86.21% specificity with 95% confidence interval. The results of RSA, MyGELISA and commercial ELISA kit showed antibodies positif againts M. gallisepticum. In conclusion, MyGELISA using freeze-thawing antigen can be used to detect antibodies of M. gallisepticum.

Perbandingan Dua Desinfektan dalam Mengeliminasi Virus Avian Influenza H5N1 pada Telur Tetas Umar Suryanaga; Retno D. Soejoedono; Ni Luh Putu Ika Mayasari
Jurnal Sain Veteriner Vol 36, No 1 (2018): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Avian Influenza (AI) is a zoonotic viral disease in birds which demands priority on control and measures. Spread of AI virus can occur directly or indirectly. The use of disinfectant and handling of hatching egg waste into one of the actions that must be applied in hatchery to control the spread of AI virus. This research aim to compared two types of desinfectant in eliminating AI virus. The research was designed into 6 groups. Group I was SAN (Specific Antibody Negative) eggs as untreated negative control, group II was SAN egg treated by fumigation using potassium permanganate (KMnO4) and formalin in room temperature for 20 minutes, group III was SAN eggs soaked in benzalkolnium chloride (BKC) in room temperature for 30 seconds, group IV was SAN contaminated by AI H5N1 virus and fumigated by potassium permanganate and formalin in room temperature for 20 minutes, group V was SAN eggs contaminated by AI H5N1 virus and then soaked in benzalkonium chloride in room temperature for 30 second, and group VI was SAN eggs contaminated by AI H5N1 virus in room temperature for 10 minutes as positive control. AI H5N1 virus detection was done by using RT-PCR (Reverse Transcription Polymerase Chain Reaction) and confirmed by isolation in Embyronated Chicken Egg. The result of this research showed that the use of potassium permanganate and formalin disinfectant gave little better performance compared to benzalkoniun chloride in eliminating AI H5N1 virus on hatching eggs.

Deteksi Gen Penyandi Resistensi ampC dan mcr-1 pada Escherichia coli penyebab Colibacillosis Unggas di Sukabumi (DETECTION OF GENE ENCODING RESISTANCE AMPC AND MCR-1 IN ESCHERICHIA COLI CAUSES AVIAN COLIBACILLOSIS IN SUKABUMI) Agustin Indrawati; Ryan Septa Kurnia; Ni Luh Putu Ika Mayasari
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Antibiotic resistance has become a global problem that can threaten human and animal health. The use of antibiotics in livestock as a treatment and control of disease is often associated with the cause of the spread of resistant bacteria. Resistance bacteria are caused by presence of resistant gene resistance that can move between bacteria. This study aims to detect the presence of genes that encode resistance to ampicillin (ampC) antibiotics and colistin (mcr-1) in Escherichia coli bacteria derived from cases of colibacillosis in Sukabumi. A total of 25 isolates of E. coli archive collection of PT. Medika Animal Lab is used in this research. All isolates identified using PCR were then tested for sensitivity using the disk diffusion method and minimum inhibitory concentrations (MICs). Isolates that are resistant to ampicillin and colistin were tested for detection of ampC and mcr-1 genes using PCR. The results of the sensitivity test showed the whole isolates were resistant to ampicillin (100%) and phosphomycin (8%), but none were resistant to colistin sulphate. The total isolate E. coli successfully detected gene encoding resistance of ampC (100%). The results of sensitivity and resistance detection test showed that the whole isolates were ampicillin resistant and had the ampC resistance-encoding gene.

MULTIDRUG RESISTANCE OF Klebsiella pneumoniae IN CATS IN BOGOR, INDONESIA Juliadi Ramadhan; Safika Safika; Ni Luh Putu Ika Mayasari
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

This study aims to measure the level of antibiotic resistance to Klebsiella pneumoniae isolated from clinical cats in Bogor. Samples were isolated and identified macroscopically, microscopically, and biochemically. Positive isolates were tested for antibiotic sensitivity using the Kirby-Bauer disk diffusion method. The results showed that Klebsiella pneumoniae isolated from sputum and laryngeal swabs of clinic cats in Bogor had experienced Multidrug Resistance (MDR). The highest level of resistance to Klebsiella pneumoniae occurred in the β-lactam group (amphicillin 76%) followed by the tetracycline group (oxytetracycline 72% and tetracycline 68%), then the quinolone group (enrofloxacin 52%), and finally the aminoglycoside group (gentamicin 44%). The results of this study are expected to be taken into consideration in the use of antibiotics for the treatment of cases related to the Klebsiella pneumoniae bacteria.

IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF BOVINE HERPERVIRUSES (BoHV) DNA TERMINASE PARTIAL GENE IN ACEH CATTLE Lilik Prayitno; Uus Saepuloh; Ni Luh Putu Ika Mayasari; Faisal Faisal; Ellis Dwi Ayuningsih; Joko Pamungkas Pamungkas
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Bovine Herpesvirus (BoHV) is a member of Herpesviridae family that acts as pathogenic virus causing infectious bovine rhinotracheitis (IBR) among cattles, resulting in economic loss for cattle industry. BoHV-1 infection in cows is closely related to abortion, respiratory infection, reduced milk production, infertility, and low birth weight. The aim of this study was to identify and characterize the molecular of BoHV-1 and other virus types, as well as the possible presence of other Herpesviridae family using PCR to amplify DNA terminase gene. Four out of 210 nose swab samples were positive for herpes virus on DNA terminase gene. Further characterization of samples showed 99-100% similarity to BoHv-1 and BoHV-6 sequence. Genetic distance between genera BoHV-1 and BoHV-6 is 0.518 and within genera was 0.001 and 0.044. According to phylogenetic tree analysis of DNA terminase gene, the analyzed sequence clustered into 2 genera, namely Varicellovirus which is identical to BoHV-1 and Macavirus which is identical to BoHV-6. The study provides scientific information on molecular characteristics of Herpesviridae family, especially BoHV-1 which is prevalent in Indonesia with the highest density in the central ranches in Aceh province

MULTIDRUG RESISTANCE OF Klebsiella pneumoniae IN CATS IN BOGOR, INDONESIA Juliadi Ramadhan; Safika Safika; Ni Luh Putu Ika Mayasari
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i2.17882

Â This study aims to measure the level of antibiotic resistance to Klebsiella pneumoniae isolated from clinical cats in Bogor. Samples were isolated and identified macroscopically, microscopically, and biochemically. Positive isolates were tested for antibiotic sensitivity using the Kirby-Bauer disk diffusion method. The results showed that Klebsiella pneumoniae isolated from sputum and laryngeal swabs of clinic cats in Bogor had experienced Multidrug Resistance (MDR). The highest level of resistance to Klebsiella pneumoniae occurred in the Î²-lactam group (amphicillin 76%) followed by the tetracycline group (oxytetracycline 72% and tetracycline 68%), then the quinolone group (enrofloxacin 52%), and finally the aminoglycoside group (gentamicin 44%). The results of this study are expected to be taken into consideration in the use of antibiotics for the treatment of cases related to the Klebsiella pneumoniae bacteria.

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