Articles
<![CDATA[The Effect of Bitter Melon Extract (Momordica charantia)in Inhibition of NFkB Activation in Leptin treated HUVECS ]]>
<![CDATA[Purwandhono, Azham]]>;
<![CDATA[Bumi, Candra]]>
<![CDATA[ UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014 ]]>
Publisher : <![CDATA[UNEJ e-Proceeding ]]>
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<![CDATA[The obesityincreases the riskof morbidityandmortalityof type2diabetesandcardiovasculardisease. Leptin, an adipocytokine that regulate metabolic process, was increase in obesity. Hyperleptinemia activate the transcriptionfactorNuclearFactorKappaB(NF-kB), leading to the occurrence of inflammation. The chronic inflammation in vessels would cause the dysfunction of endothelial cell.Bitter melon (Momordica charantia) has beenused by many peoplefor the treatmentof diseases. Studies showthatbitter melon fruitcontainssaponins(charantins) andalkaloids. This study aimed toinvestigate the effect of bitter melon extract on the level of activated NF-κB. Cultured endothelial cell, HUVECs (Human Umbilical Vein Endothelial), is used for a model of endothelial cells. There are 5 groups: 1) HUVECs culture without leptin as negative control; 2) HUVECs culture with leptin as positive control; and 3 groups given various dosages of bitter melon (0.1 mg/ml; 0.05 mg/ml; 0.01 mg/ml; 0.005 mg/ml; 0.001 mg/ml) and Leptin. Leptin (500 ng/ml) is added on HUVECs to stimulate responds of endothelial cells mimicking obesity condition. Momordica charantiawere given 2 h before leptin treatment. The level of activated NF-KB was measured by ELISA. This study showed that leptin significantly induce NFkB activation and the treatment of bitter melon inhibit NFkB activation significantly (p<0.05). Keywords: Leptin, Endothel, bitter melon, NFkB]]>
<![CDATA[OUTER MEMBRANE PROTEIN OF PORPHYROMONAS GINGIVALIS AS BASIC OF DIAGNOSTIC TOOL IN AGGRESSIVE PERIODONTITIS: PROTEIN MEMBRAN BAGIAN LUAR PORPHYROMONAS GINGIVALIS SEBAGAI ALAT DIAGNOSTIK PERIODONTITIS AGRESIF ]]>
<![CDATA[Desi Sandra Sari]]>;
<![CDATA[Candra Bumi]]>;
<![CDATA[Yuliana Mahdiyah DA]]>
<![CDATA[ Dentika: Dental Journal Vol. 16 No. 2 (2011): Dentika Dental Journal ]]>
Publisher : <![CDATA[TALENTA ]]>
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DOI: 10.32734/dentika.v16i2.1831
<![CDATA[Porphyromonas gingivalis is a gram-negative anaerobic bacteria and has been shown previously to be one of the major pathogens in aggressive periodontitis. Outer membrane protein is the major virulence factor of P. gingivalis and plays role in the host immune response impair againts P. gingivalis, which in turns, causing tissue destruction and bone resorption. This study was aimed to investigate the isolation and characteristic outer membrane protein of P. gingivalis. Protein of OMP P. gingivalis ATCC 33277 was isolated using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and than continued by hemaglutination test. The result showed that protein profil in SDSPAGE of OMP protein was 40 kDa molecular weight dan hemaglutination test was positive in titer of hemaglutination 1/8.In conclusion, outer membrane protein molecular mass of 40kDa produced by P. gingivalis is a key virulence factor involved in the co-aggregation activity of P. gingivalis]]>
<![CDATA[Perancangan Dan Pengembangan Website Covid Care Di Jember ]]>
<![CDATA[Oktalia Juwita]]>;
<![CDATA[Priza Pandunata]]>;
<![CDATA[Bayu Taruna Widjaja Putra]]>;
<![CDATA[Candra Bumi]]>;
<![CDATA[Aditya Kurniawan]]>
<![CDATA[ TEKIBA : Jurnal Teknologi dan Pengabdian Masyarakat Vol. 1 No. 2 (2021): TEKIBA : Jurnal Teknologi dan Pengabdian Masyarakat ]]>
Publisher : <![CDATA[Fakultas Teknik, Universitas PGRI Banyuwangi ]]>
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DOI: 10.36526/tekiba.v1i2.1600
<![CDATA[Lots of information that is spread in the community about Covid1-19 needs to be confirmed so as not to disturb the public. Many people have not been able to distinguish between true and false information. There are still many people in Jember who have not received accurate and reliable information about the current Covid vaccine. It's not just about how to get vaccinated, but the requirements that must be met in order to get vaccinated in Jember, also have not been conveyed properly to the public. In view of this, we need a means that can facilitate the dissemination of such information properly. This service activity resulted in a website with the name CovidCare. CovidCare provides information to assist the University of Jember's covid task force in collecting data on UNEJ residents who have been vaccinated and also exposed to Covid. The CovidCare website can also make it easier for the Covid task force to accept students who want to register as Covid volunteers who are part of the Thematic KKN at UNEJ. In addition to these internal parties, the CovidCare website can also be used by outside parties (health workers) in registering the Covid vaccine.]]>
<![CDATA[Contraception Reduces Breast Milk Protein Level of Breastfeeding Mothers in Jember, East Java, Indonesia ]]>
<![CDATA[Nuzula Irfa Nuriana]]>;
<![CDATA[FX. Ady Soesetijo]]>;
<![CDATA[Candra Bumi]]>
<![CDATA[ Health Notions Vol 4, No 5 (2020): May ]]>
Publisher : <![CDATA[Humanistic Network for Science and Technology (HNST) ]]>
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DOI: 10.33846/hn40504
<![CDATA[The protein level of breast milk affected the growth and development of infants. Protein has special function which is irreplaceable by other nutrients to form and protect the body cells. Protein also plays a role as antibody, functions as the body defense mechanism against various diseases and infections. Infant death in Indonesia is mostly caused by respiratory tract infections and diarrhea. Breastfeeding can help to prevent the occurrence of morbidity and mortality of infants and toddlers in which the breast milk contains many essential nutrients needed by the body, one of them is protein. The aim of this research was to analyze the effect of contraception on breast milk protein level of breastfeeding mothers in Jember regency. The design of this research was observational analytic with cross sectional approach, using statistic test of logistic regression. The result of the research showed that the effect of contraception on the protein level of breast milk obtained p-value=0.021 with Exp(B)=566.263. Based on the research, it can be concluded that there was an effect of contraception on breast milk protein level. Keywords: infection; breastmilk protein; contraception]]>
<![CDATA[Utilization of viable bone marrow derivat stem cells through an adaption in low oxigen tension as an attempt to increase cellular transplantation efficacy for spermatogenesis process ]]>
<![CDATA[Erma Safitri]]>;
<![CDATA[Suzanita Utama]]>;
<![CDATA[Candra Bumi]]>;
<![CDATA[Sri Wigati Mardi]]>;
<![CDATA[. Mulyani]]>;
<![CDATA[. Helen]]>;
<![CDATA[. Purwati]]>;
<![CDATA[R. Heru Prasetyo]]>;
<![CDATA[Mas’ud Hariadi]]>;
<![CDATA[Fedik Abdul Rantam]]>
<![CDATA[ Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences ]]>
Publisher : <![CDATA[Syiah Kuala University ]]>
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<![CDATA[Cellular transplantation using stem cells provides very promising solutions in the regeneration and repairment of cells that have experienced degeneration where recovery through medical or surgical intervension is impossible. However, the very low viability of transplanted stem cells limits the transplantation efficacy. The aim of this research was to obtain viable bone marrow derived stem cells by an adaptation treatment in a low oxygen tensioned in vitro culture. Low oxygen tension adaptation was adjusted to the niche of the stem cells in vivo. In this study, in vitro culture of stem cells in 1% oxygen was compared to those of the conventional culture in 21 % oxygen.Results showed that under 1% oxygentension cell proliferation was slower with larger or rounded triangle shaped cells, and senescence or dead cells was low. Meanwhile under 21 % oxygen tension cell proliferation was two fold faster with flattened and slender cells, and senescence or dead cells was higher. In conclusion, conventional in vitro culture under 21% oxygen caused cell aging (senescence) and rapid cell death, therefore the transplanted cells were not viable.]]>
<![CDATA[Pelaksanaan Wajib Notifikasi (WiFi) TB di Dinas Kesehatan Kabupaten Jember ]]>
<![CDATA[Fiya Wadudah]]>;
<![CDATA[Irma Prasetyowati]]>;
<![CDATA[Candra Bumi]]>
<![CDATA[ Pustaka Kesehatan Vol 8 No 2 (2020): Volume 8 No. 2, 2020 ]]>
Publisher : <![CDATA[UPT Percetakan dan Penerbitan Universitas Jember ]]>
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DOI: 10.19184/pk.v8i2.20360
<![CDATA[Indonesia mengikuti target eliminasi global yaitu Eliminasi TB 2035 dan Indonesia bebas TB 2050. Tantangan dalam program pengendalian Tuberkulosis di Indonesia adalah banyaknya kasus tuberkulosis yang tidak terdeteksi maupun kasus yang sudah terdeteksi namun belum terlaporkan, sehingga perlu dilakukan perubahan pendekatan penemuan kasus, dari secara pasif menjadi intensif dan aktif. Rumitnya pengisian form TB oleh dokter swasta, membuat rendahnya notifikasi pasien TB. Maka dibuat aplikasi berbasis smartphone yaitu Aplikasi Wajib Notifikasi TB (WiFi) TB yang lebih sederhana dan mudah digunakan. Penelitian ini bertujuan untuk menggambarkan pelaksanaan wajib noifikasi (WiFi) TB di Dinas Kesehatan Kabupaten Jember. Penelitian ini menggunakan jenis penelitian deskriptif. Sampel penelitian adalah 2 petugas TB. Penelitian ini dianalisis menggunakan analisis deskriptif. Hasil dari penelitian ini menunjukkan bahwa gambaran dari aspek input, proses, dan output dalam pelaksanaan wajib notifikasi (WiFi) TB]]>
<![CDATA[Evaluasi Cold Chain Management Vaksin di Puskesmas Kabupaten Jember ]]>
<![CDATA[Abdul Syakur]]>;
<![CDATA[Christyana Sandra]]>;
<![CDATA[Candra Bumi]]>
<![CDATA[ Jurnal Manajemen Kesehatan Indonesia Vol 9, No 1 (2021): April 2021 ]]>
Publisher : <![CDATA[Magister Kesehatan Masyarakat, Fakultas Kesehatan Masyarakat, Universitas Diponegoro ]]>
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DOI: 10.14710/jmki.9.1.2021.21-27
<![CDATA[Immunization is the most cost-effective health prevention method to suppress the incidence of vaccine-preventable disease. Immunization Program in Jember district is still not optimal with many vaccine-preventable disease cases found. Another problem found in Jember is the management of logistics vaccine that is still not optimal. Based on the activity plan report of ORI Difteri 2018, it is known that the logistics needs of some vaccines do not comply with the allocation received by Jember regency of the province. This condition This was a descriptive study using questionaire and observations data as well as the documentation study of the annual Report of Health Pharmacy Installation (IFK) year 2019. Respondent to this study were immunization officers at primary health care of Jember District. The result of cold chain management evaluation is known that the most are good enaugh. In some primary health care are still hampered insufficient means so that the maintenance of vaccine is still not optimalBased on the result that cold chain management evaluation, the knowledge officer on cold chain management is still lacking good. In the some primary health care, it is known that the equipment canstraints are unavailable. While in the conformity of temperature only one primary helath care is not recommended. And stirage activities are mostly in accordance with SOP. ]]>
<![CDATA[The Effect of Bitter Melon Extract (Momordica charantia)in Inhibition of NFkB Activation in Leptin treated HUVECS ]]>
<![CDATA[Azham Purwandhono]]>;
<![CDATA[Candra Bumi]]>
<![CDATA[ UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014 ]]>
Publisher : <![CDATA[UPT Penerbitan Universitas Jember ]]>
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<![CDATA[The obesityincreases the riskof morbidityandmortalityof type2diabetesandcardiovasculardisease. Leptin, an adipocytokine that regulate metabolic process, was increase in obesity. Hyperleptinemia activate the transcriptionfactorNuclearFactorKappaB(NF-kB), leading to the occurrence of inflammation. The chronic inflammation in vessels would cause the dysfunction of endothelial cell.Bitter melon (Momordica charantia) has beenused by many peoplefor the treatmentof diseases. Studies showthatbitter melon fruitcontainssaponins(charantins) andalkaloids. This study aimed toinvestigate the effect of bitter melon extract on the level of activated NF-κB. Cultured endothelial cell, HUVECs (Human Umbilical Vein Endothelial), is used for a model of endothelial cells. There are 5 groups: 1) HUVECs culture without leptin as negative control; 2) HUVECs culture with leptin as positive control; and 3 groups given various dosages of bitter melon (0.1 mg/ml; 0.05 mg/ml; 0.01 mg/ml; 0.005 mg/ml; 0.001 mg/ml) and Leptin. Leptin (500 ng/ml) is added on HUVECs to stimulate responds of endothelial cells mimicking obesity condition. Momordica charantiawere given 2 h before leptin treatment. The level of activated NF-KB was measured by ELISA. This study showed that leptin significantly induce NFkB activation and the treatment of bitter melon inhibit NFkB activation significantly (p<0.05). Keywords: Leptin, Endothel, bitter melon, NFkB]]>
<![CDATA[PERAN PROTEIN MEMBRAN LUAR 55 kDa Salmonella typhi ISOLAT JEMBER SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN ]]>
<![CDATA[Diana Chusna Mufida]]>;
<![CDATA[Candra Bumi]]>;
<![CDATA[Heni Fatmawati]]>
<![CDATA[ JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 1 (2009): December 2009 ]]>
Publisher : <![CDATA[The East Java Biological Society ]]>
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DOI: 10.23869/234
<![CDATA[Salmonella typhi is an obligate pathogen that is usually found in clinical specimen from typhoid fever patients. The pathogenic mechanism of bacteria are not fully elucidated especially its potential activity of the outer membrane protein (OMP) as hemaglutinin and adhesion molecule. After identification, bacterial isolate of outer membrane protein fraction 12,5% SDS-PAGE were used to isolate OMP followed by hemaglutinin test and in vitro adhesion test. The study showed that the 55 kDa protein of S. typhi is a hemaglutinin protein that could agglutinate, Wistar mouse erythrocytes. The 55 kDa OMP is also an adhesion protein showed by its activity to adherence to Wistar mice’s enterocyt. The increased dose of 55 kDa OMP will decrease the amount of S. typhi bacteria adherenced to Wistar mice’s enterocyt. Outer membrane protein 55 kDa S. typhi as hemagglutinin and also adhesion protein.]]>
<![CDATA[Role of secretome mesenchymal stem cells as immunomodulator in periodontal diseases ]]>
<![CDATA[Desi S. Sari]]>;
<![CDATA[Ernie Maduratna]]>;
<![CDATA[Candra Bumi]]>;
<![CDATA[I-Ketut Sudiana]]>;
<![CDATA[Fedik A. Rantam]]>
<![CDATA[ Journal of Dentomaxillofacial Science Vol. 6 No. 3 (2021): (Available online: 1 December 2021) ]]>
Publisher : <![CDATA[DiscoverSys Inc. ]]>
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DOI: 10.15562/jdmfs.v6i3.989
<![CDATA[Objective: The purpose of this review literature is to look at the mechanism of mesenchymal stem cell secretome in the regeneration of periodontal tissue.Methods: This literature review was collected from several journals and research that has been done, where the application of Mesenchymal stem cells (MSCs) promises a favourable result for cell-based therapy in inflammatory diseases, immune system disorders, regeneration, and tissue repair.Results: MSCs possess a potent immunomodulatory function, paracrine effects, and the ability to differentiate into different cells. Not only mesenchymal stem cells can replace damaged tissue or cells but also secrete secretome namely bioactive ingredients such as cytokines, chemokines, growth factors, pro-inflammatory, and anti-inflammatory which play an essential role in regulating the host immune system. Periodontal disease is an inflammatory disease that occurs in the teeth supporting structures. Lately, many therapies for periodontal disease utilized MSCs from gingival tissue and periodontal ligaments. Secretome activates endogenous MSCs to improve the immune system in periodontal disease that might create a better microenvironment of periodontal tissue.Conclusion: The secretome of MSCs has attracted a lot of attention and has the potential to repair and regenerate tissues including periodontal tissue]]>
