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PENGARUH IRADIASI SINAR GAMMA TERHADAP VARIASI PERTUMBUHAN ANGGREK Brachypeza indusiata (Reichb.f) Garay SECARA IN VITRO Wardhani, Maria Utami Dewi; Puspitaningtyas, Dwi Murti; Dinarti, Diny
Buletin Kebun Raya Vol 10, No 2 (2007): Buletin Kebun Raya Vol. 10 (2) Juli 2007
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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A research was carried out in Orchid Laboratory of Bogor Botanical Gardens to observe the effect of gamma ray irradiation on the growth variation of Brachypezo indusiata (Reichb.f) Garray and suitable culture medium for propagating the plant. The research was arranged in a factorial experiment with a Completely Randomized Design. The first factor was gamma ray irradiation on protocorm-likeÂbodies (plb) of germinated seeds of B. indusiata in which four levels of irradiation was applied, i.e. 0, 10, 20, and 30 Gy. The second factor was the use of two different culture media, i.e. Vacin & Went medium supplemented with either banana pulp or a mixture of tomato and sprout bean as the essential organic compound of the medium. The result showed that the irradiation treatment improved the survival of B. indusiata explants, most significantly at 20 Gy level of irradiation, and could modify the growth of the resulted plantlets by reducing the height of the plant and the length and width of the leaves. The higher the level of irradiation the smaller the plantlets produced. Meanwhile, the addition of organic compound derived from banana fruit into the basal medium of Vacin & Went enhanced the growth of the plantlets better than that derived from a mixture of tomato fruit and bean sprout. However, there was no significant interaction effect detected between the irradiation treatments and the use of different culture media.

PENGARUH IRADIASI SINAR GAMMA TERHADAP VARIASI PERTUMBUHAN ANGGREK Brachypeza indusiata (Reichb.f) Garay SECARA IN VITRO Wardhani, Maria Utami Dewi; Puspitaningtyas, Dwi Murti; Dinarti, Diny
Buletin Kebun Raya Vol 10, No 2 (2007): Buletin Kebun Raya Indonesia Vol. 10 (2) July 2007
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

A research was carried out in Orchid Laboratory of Bogor Botanical Gardens to observe the effect of gamma ray irradiation on the growth variation of Brachypezo indusiata (Reichb.f) Garray and suitable culture medium for propagating the plant. The research was arranged in a factorial experiment with a Completely Randomized Design. The first factor was gamma ray irradiation on protocorm-likeÂbodies (plb) of germinated seeds of B. indusiata in which four levels of irradiation was applied, i.e. 0, 10, 20, and 30 Gy. The second factor was the use of two different culture media, i.e. Vacin & Went medium supplemented with either banana pulp or a mixture of tomato and sprout bean as the essential organic compound of the medium. The result showed that the irradiation treatment improved the survival of B. indusiata explants, most significantly at 20 Gy level of irradiation, and could modify the growth of the resulted plantlets by reducing the height of the plant and the length and width of the leaves. The higher the level of irradiation the smaller the plantlets produced. Meanwhile, the addition of organic compound derived from banana fruit into the basal medium of Vacin & Went enhanced the growth of the plantlets better than that derived from a mixture of tomato fruit and bean sprout. However, there was no significant interaction effect detected between the irradiation treatments and the use of different culture media.

ANALISIS BIOINFORMATIKA BERBASIS WEB PADA SEKUEN GENOM PARSIAL SAGU (Metroxylon sagu Rottb.) Purwoko, Devit; Cartealy, Imam Civi; Tajuddin, Teuku; Dinarti, Diny; Sudarsono, Sudarsono
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 1 (2018): June 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

WEB-based bioinformatic analysis on partial genome sequence of Sago (Metroxylon sagu Rottb.)ABSTRACTSago genome sequencing analysis is still very limited. This study is a preliminary study of sago sequence analysis obtained from NGS technology to understand and identify new genetic sequences that have homology to genes in the NCBI database. Sequences were analyzed using Blast2Go to determine the genetic function annotation, putative gene identification was performed on the Arabidopsis database using the BLASTx program with a 10-3 e-value limit on The Arabidopsis Information Resource (TAIR) (http://www.arabidopsis.org/index.jsp). Gene interactions were analyzed using DAVID and GeneMania programs. Based on sequence analysis with Blast2Go, 33 sequences with Blastx hit consisting of: 29 sequences had a high homology. The sago sequences with a similarity of ≥ 90% are glutamate decarboxylase and HT1-like serine threonine kinase with hit number 10. The distribution of interactions between genes from GeneMania analysis is known to be mostly interconnected in the 65.13% protein domain, predicted 19.83%, genes with 14.47% shared expression and the remaining 0.57% had localization together.Keywords: bioinformatics, gene annotation, gene ontology, genome sequence, Metroxylon sagu ABSTRAKKajian analisis sekuen genom sagu hingga saat ini masih amat terbatas. Penelitian ini merupakan riset pendahuluan analisis sekuen sagu yang diperoleh dari teknologi NGS untuk mengetahui dan mengidentifikasi sekuen gen baru yang memiliki homologi dengan gen pada database NCBI. Sekuen dianalisis menggunakan perangkat Blast2Go untuk mengetahui anotasi fungsional gen, identifikasi gen putatif dilakukan terhadap database Arabidopsis menggunakan program BLASTx dengan batas e-value 10-3 pada The Arabidopsis Information Resource (TAIR). Interaksi gen dianalisis menggunakan program DAVID dan GeneMania. Berdasarkan analisis sekuen dengan Blast2Go, diperoleh 33 sekuen dengan Blastx hit yang terdiri atas: 29 sekuen memiliki homologi yang tinggi. Gen dengan rataan kemiripan ≥ 90% adalah glutamate decarboxylase dan serine threonine-kinase HT1-like dengan jumlah hit 10. Persebaran interaksi antar gen hasil analisis GeneMania diketahui sebagian besar saling terkait pada domain protein 65,13%, koneksi yang berhasil diprediksi 19,83%, gen dengan ekspresi bersama 14,47% dan sisanya 0,57% memiliki peranan bersama. Kata Kunci: anotasi gen, bioinformatika, Metroxylon sagu, ontologi gen, sekuen genome

In Vitro Mutagenesis of Etlingera elatior by Gamma Ray Intermittent Irradiation Azzahra, Elmi Irmayanti; Aisyah, Syarifah Iis; Dinarti, Diny; Krisantini, Krisantini
Journal of Tropical Crop Science Vol 5 No 3 (2018): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Etlingera elatior is tropical ornamental plant commonly called “torch ginger” from Zingiberaceae family. Conventional breeding of E. elatior is limited by cross incompatibility, poor fruit set and low seed production. In this study irradiation of E. elatior with Gamma ray performed to induce mutation. This study was aimed to increase morphological diversity and to obtain unique morphological characters to increase the aesthetic value of E. elatior as ornamental plants and cut flower. Two genotypes of E. elatior, red and white flowers, were tested. The LD20, LD35, and LD50 were determined following intermittent (split dose) Gamma irradiation with a two-hour gap between each gamma ray shot. Red genotype E. elatior explants were irradiated with dose of 3 + 3 Gy (LD20); 4 + 4 Gy (LD35) and 5 + 5 Gy (LD50); white genotype were irradiated with a dose of 2 + 2 Gy (LD20); 2.8 + 2.8 Gy (LD35) and 3.7 + 3.7 Gy (LD50). Non-irradiated explants were set as control. The results of this study indicated that the increase in dose of gamma ray irradiation changed shoot length and number of leaves in the red genotype per explant as compared to control. Morphological changes occurred in leaf shape at 5 + 5 Gy and 3.7 + 3.7 Gy and formation of variegated leaves at 2.8 + 2.8 Gy and 5 + 5 Gy. Keywords: mutation breeding, ornamental plant, split irradiation dose, torch ginger, Zingiberaceae

Aplikasi Ribavirin Pada Shoot Tip Bawang Merah untuk Eliminasi Virus OYDV (Application of Ribavirin on Shoot Tip of Shallot for OYDV Eradication) Aqlima, nFN; Purwoko, Bambang Sapto; Hidayat, Sri Hendrastuti; Dinarti, Diny
Jurnal Hortikultura Vol 28, No 2 (2018): Desember 2018
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v28n2.2018.p251-258

Kemoterapi merupakan aplikasi senyawa kimia (ribavirin) yang memiliki aktivitas antiviral guna menghambat ataupun menghentikan multiplikasi virus pada jaringan tanaman. Penelitian bertujuan mengetahui pengaruh konsentrasi ribavirin terhadap pertumbuhan shoot tip bawang merah untuk mengeliminasi virus OYDV. Penelitian dilaksanakan di Laboratorium Kultur Jaringan 3, Departemen AGH IPB dan Laboratorium Virologi Tumbuhan, Departemen Proteksi Tanaman IPB, sejak bulan Oktober 2015 hingga Juni 2016. Rancangan percobaan yang digunakan adalah kelompok lengkap teracak dengan dua faktor dan empat ulangan, setiap ulangan terdiri atas empat tabung kultur yang ditanam satu eksplan. Percobaan dilakukan secara terpisah pada dua kultivar bawang merah, yaitu Bima Brebes dan Tiron. Faktor pertama adalah konsentrasi ribavirin, yaitu 0, 5, 10, 15, dan 20 mg/L. Faktor kedua adalah ukuran eksplan (shoot tip), yaitu 1,1â€“2,0 mm dan 2,1â€“3,0 mm. Hasil penelitian menunjukkan bahwa peningkatan konsentrasi ribavirin menekan tinggi tunas cv. Bima Brebes, waktu muncul daun, tinggi tunas, dan jumlah daun cv. Tiron. Ukuran shoot tip yang lebih besar (2,1â€“3,0 mm) dapat meningkatkan persentase tumbuh eksplan dan mempercepat waktu muncul daun pada cv. Bima Brebes dan Tiron. Konsentrasi ribavirin yang diaplikasikan pada dua ukuran shoot tip masih belum dapat mengeliminasi virus OYDV pada kedua kultivar bawang merah.KeywordsBawang merah; In vitro; Ribavirin; Shoot tipAbstractChemotherapy is an apllication of chemistry compound (ribavirin) that has antiviral activity to inhibit virus multiplication in plant tissues. The objective of the experiment was to evaluate the effect of ribavirin concentrations on shoot tip growth of shallot for OYDV eradication. The experiment was cunducted at Tissue Culture Laboratory 3, Agronomy Department and Plant Virology Laboratory, Plant Protection Department of IPB from October 2015 until June 2016. The experiment was arranged in a completely rendomized block design with two factors and four replications. Each experimental unit consisted of four bottles with one explant in it. The first factor was ribavirin concentrations, i.e. 0, 5, 10, 15, and 20 mg/L. Second factor was shoot tip sizes, i.e. 1.1â€“2.0 mm and 2.1â€“3.0 mm. The experiment showed that increasing ribavirin concentrations suppressed the shoot length of cv. Bima Brebes, and it also inhibited the time of leaf to emerge, shoot length, and leaf number of cv. Tiron. Increasing shoot tip size (2.1â€“3.0 mm) influenced percentage of explant growth and speed the time of leaf emergance of cv. Bima Brebes and Tiron. Ribavirin concentrations used in this treatment did not eradicate OYDV in both shoot tipe sizes of the two cultivars.

ANALISIS BIOINFORMATIKA BERBASIS WEB PADA SEKUEN GENOM PARSIAL SAGU (Metroxylon sagu Rottb.) Purwoko, Devit; Cartealy, Imam Civi; Tajuddin, Teuku; Dinarti, Diny; Sudarsono, Sudarsono
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 1 (2018): June 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

WEB-based bioinformatic analysis on partial genome sequence of SagoÃ‚Â (Metroxylon sagu Rottb.)ABSTRACTSago genome sequencing analysis is still very limited. This study is a preliminary study of sago sequence analysis obtained from NGS technology to understand and identify new genetic sequences that have homology to genes in the NCBI database. Sequences were analyzed using Blast2Go to determine the genetic function annotation, putative gene identification was performed on the Arabidopsis database using the BLASTx program with a 10-3 e-value limit on The Arabidopsis Information Resource (TAIR) (http://www.arabidopsis.org/index.jsp). Gene interactions were analyzed using DAVID and GeneMania programs. Based on sequence analysis with Blast2Go, 33 sequences with Blastx hit consisting of: 29 sequences had a high homology. The sago sequences with a similarity of Ã¢â€°Â¥ 90% are glutamate decarboxylase and HT1-like serine threonine kinase with hit number 10. The distribution of interactions between genes from GeneMania analysis is known to be mostly interconnected in the 65.13% protein domain, predicted 19.83%, genes with 14.47% shared expression and the remaining 0.57% had localization together.Keywords: bioinformatics, gene annotation, gene ontology, genome sequence, Metroxylon saguÃ‚Â ABSTRAKKajian analisis sekuen genom sagu hingga saat ini masih amat terbatas. Penelitian ini merupakan riset pendahuluan analisis sekuen sagu yang diperoleh dari teknologi NGS untuk mengetahui dan mengidentifikasi sekuen gen baru yang memiliki homologi dengan gen pada database NCBI. Sekuen dianalisis menggunakan perangkat Blast2Go untuk mengetahui anotasi fungsional gen, identifikasi gen putatif dilakukan terhadap database Arabidopsis menggunakan program BLASTx dengan batas e-value 10-3 pada The Arabidopsis Information Resource (TAIR). Interaksi gen dianalisis menggunakan program DAVID dan GeneMania. Berdasarkan analisis sekuen dengan Blast2Go, diperoleh 33 sekuen dengan Blastx hit yang terdiri atas: 29 sekuen memiliki homologi yang tinggi. Gen dengan rataan kemiripan Ã¢â€°Â¥ 90% adalah glutamate decarboxylase dan serine threonine-kinase HT1-like dengan jumlah hit 10. Persebaran interaksi antar gen hasil analisis GeneMania diketahui sebagian besar saling terkait pada domain protein 65,13%, koneksi yang berhasil diprediksi 19,83%, gen dengan ekspresi bersama 14,47% dan sisanya 0,57% memiliki peranan bersama.Ã‚Â Kata Kunci: anotasi gen, bioinformatika, Metroxylon sagu, ontologi gen, sekuen genomeÃ‚Â

Increasing Ploidy Level of Garlic (Allium sativum L.) “Tawangmangu Baru” In-Vitro Using Colchicine Hailu, Molla Gebreyohannes; Wiendi, Ni Made Armini; Dinarti, Diny
Journal of Tropical Crop Science Vol 7 No 03 (2020): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.7.03.122-136

“Tawangmangu Baru” garlic variety is known to have low productivity. The variety is still highly demanded due to its strong flavour and aroma; however, its production has not yet been able to fulfill the local needs of Central Java due to the small size and limited production area. This study aimed to determine the effect of concentration and time duration of colchicine treatment towards increasing the ploidy level of “Tawangmangu Baru” garlic variety for genetic variability. The experimental design used in this study was a complete randomized design with two factorials and 12 combinations. The first factor was concentration of colchicine, i.e. 0.00, 0.02, 0.04, 0.06, 0.08 and 0.10%, and the second factor was the immersion time, i.e. 24 and 48 hours. The result indicated that, 4.72% callus induction was obtained in BDS + 0.4 mg.L-1 2,4-D + 2.0 mg.L-1 kinetin; and 4.0% callus proliferation were obtained in both BDS + 1.5 mg.L-1 2,4-D + 1.0 mg.L-1 kinetin and MS +1.5 mg.L-1 2,4-D and 1.0 mg.L-1 kinetin. The untreated plantlets showed higher mortality rate compared to the explants with 48 hours colchicine treatment. Higher number of shoots were recorded in 0.1% colchicine at 48 hours and lower shoots in 24 hours, whereas 0.1% colchicine at 24 and 48 hours showed the highest ploidy level of total nuclear DNA analyzed by flow cytometry. The genetic diversity of the “Tawangmangu Baru” garlic was successfully enhanced by colchicine and immersion treatment. Mutant lines with tetraploid and mixoploid plants were obtained. The putative lines obtained at 0.1% colchicine treatment were subcultured to produce new mutants before testing the phenotype. The application of colchicine at 24 and 48 hours treatment improved the genetic potential of “Tawangmangu Baru” garlic variety in vitro. The application of colchicine increased the ploidy level and an increase in ploidy is expected to make the bulb size larger. Larger tuber size will increase the tuber weight, and also the overall garlic productivity and production in the future.

Potensi Hasil dan Keragaman Fenotipik Aren (Arenga pinnata (Wurmb) Merr.) Sulawesi Tenggara Fatsan, Ahmad; Sudarsono, Sudarsono; Dinarti, Diny; Maskromo, Ismail
Berkala Penelitian Agronomi Vol 8, No 2 (2020)
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/bpa.v8i2.14544

Aren adalah salah satu jenis palma yang penyebarannya sangat luas di Indonesia, termasuk di provinsi SulawesiTenggara. Pohon aren memiliki banyak manfaat mulai dari akar sampai daun. Bunga aren berbentuk mayang denganuntaian yang berlapis-lapis. Pengamatan fenotipik dan potensi nira aren dilakukan di tiga Kabupaten yaitu Muna, KolakaTimur dan Bombana.Pengamatan potensi aren Sulawesi Tenggara dilakukan dengan cara mengambil sampel 5 pohonaren di Kabupaten Muna, Kolaka Timur dan Bombana. Parameter karakter fenotipik meliputi lingkar batang dankomponen buah serta potensi poduksi. Hasil uji t-test pengamatan fenotipik aren Sulawesi Tenggara menunjukkanperbedaan yang signifikan pada karakter-karakter fase generatif, terutama aren Muna. Aren Bombana memiliki %brix20.6% lebih baik bila dibandingkan dengan aren Muna dan Kolaka Timur. Produksi rata-rata nira Muna lebih tinggidibandingkan kabupaten lainnya dengan rata-rata produksi23.64 l/mayang/hari sedangkan produksi rata-rata arenSulawesi Tenggara 20.65-23.64 l/mayang/hari.Kata kunci : Aren, Brix, Fenotipik, Generatif,Mayang, Nira

Pengaruh Umur Embrio dan Jenis Media Dasar Terhadap Keberhasilan Embryo Rescue Aren (Arenga pinnata (Wurmb) Merr.) secara In Vitro ARSYAD, MIRZA ARSIATY; SUDARSONO, nFn; PURWITO, AGUS; DINARTI, DINY
Buletin Palma Vol 14, No 1 (2013): Vol. 14 No. 1, Juni 2013
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bp.v14n1.2013.20-27

Tanaman aren (Arenga pinnata (Wurmb) Merr.) adalah salah satu jenis palma yang bernilai ekonomi tinggi. Salah satu aspek penting dalam pengembangan tanaman aren adalah penyediaan bibit. Embryo rescue diharapkan dapat menjadi alternatif solusi penyediaan bibit aren. Penelitian yang dilakukan bertujuan untuk 1) mengetahui pengaruh umur embrio zigotik terhadap perkecambahan serta perkembangan kecambah dalam embryo rescue aren, dan 2) untuk menguji efektifitas dua komposisi media dasar yang digunakan dalam kultur embrio zigotik aren secara in vitro. Penelitian ini dilaksanakan di Laboratorium Biologi dan Molekuler Tanaman, IPB dari Oktober 2011 sampai Oktober 2012. Penelitian disusun dalam bentuk percobaan faktorial menggunakan Rancangan Acak Lengkap (RAL) dengan lima ulangan. Embrio zigotik yang berasal dari buah aren muda (Â±15 bulan) dan tua (Â±30 bulan) dikultur pada media dasar Y3 dan WPM tanpa penambahan zat pengatur tumbuh (ZPT). Hasil yang diperoleh menunjukkan bahwa kemampuan hidup dan perkecambahan embrio zigotik aren terbaik diperoleh pada eksplan embrio muda (92%) dibandingkan embrio tua (72%). Komposisi media dasar (Y3 dan WPM) tidak berpengaruh nyata terhadap perkecambangan embrio aren. Haustorium dan apokol dalam kultur in vitroada yang berkembang menjadi bentuk-bentuk abnormal dengan persentase hingga 32% untuk haustorium dan 26% untuk apokol. Perkembangan haustorium dan apokol abnormal tidak berpengaruh terhadap planlet yang terbentuk dari embrio zigotik. Persentase planlet yang terbentuk dari embrio zigotik aren berkisar 6%-25% pada umur embrio zigotik dan jenis media yang diuji. Kata kunci : Aren, embrio muda, embrio tua, WPM, Y3.ABSTRACTInfluence of Embryo Age and Type of Basal Mediumto The Success of In Vitro Embryo Rescue of Sugar Palm (Arenga pinnata (Wurmb) Merr.)Sugar palm is one of the palms that has high economic value. Provision of seedling is one of important aspect to develop plant, include sugar palm (Arenga pinnata (Wurmb) Merr.) for cultivation and breeding program. Embryo rescueis expected tobe an alternative solution on provision of sugar palm seedlings. The objectives of the experiment were 1) to evaluate the effect of zygotic embryo age on germination and development of sugar palm rescued zygotic embryoand 2) to evaluate the effect of basal medium composition on sugar palm embryo culture. The experiment was conducted at Plant Molecular and Biology Laboratory of IPB, from October 2011 to October 2012. The experiment was compiled in the form of factorial experiment using Completely Randomized Design (CRD) with five replications. Zygotic embryos derived from immature (Â± 15months) and mature sugar palm fruit (Â± 30 months) were cultured on Y3 and WPM mediumin absence of plant growth regulators. The result showed that the best viability and germination of zygotic embryo obtained on immature zygotic embryo (92%) compare to the mature embryos (72%). The composition of the basal medium (Y3 and WPM) did not significantly affect on embryo germination. There was evidence that haustorium and cotyledonary petiole in in vitro culture that grown into abnormal shapes with percentages up to 32% and 26% respectively. Abnormality of haustorium and cotyledonary petiole development did not affect on plantlets derived zygotic embryos. Percentage of plantlets obtained from zygotic embryos was 6%-25% at the age ofzygotic embryo and types of basal medium that were tested.

Pengaruh BAP dan Sukrosa terhadap Perbanyakan Jahe Empirit (Zingiber officinale Rosc var. amarun) secara In Vitro Marai Rahmawati; Sandra A. Aziz; Diny Dinarti; Dodo R. Sastra
Indonesian Journal of Agronomy Vol. 32 No. 3 (2004): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The need to produce numerous and good quality plantlet in short time has been carried out with in vitro culture. The objective of this research was to study BAP and sucrose effect on the in vitro multiplication of small ginger. Research was done in Plant Tissue Culture Laboratory, Department of Agronomy. Bogor Agricultural University from November 2002 until August 2003. The treatment used BAP 0, 1, 2, 3, 4, 5 ppm and sucrose 20, 30, 40, 50 g/l. The result showed that sucrose significantly influenced shoot number in 2-5 and 8 Week After Planting (WAP) and leaf number in 2-7 WAP; root length, root number and explant fresh weight. BAP only significantly influenced leaf number in 7 WAP and root quality. With time shoot color changed from green to yellow. Higher RAP and sucrose concentration increased micro rhizome percentage. Sucrose 40 g/l or RAP 2 ppm gave numerous shoot and high explant fresh weight. Key words : Zingiber officinale Rosc var. amarun, In vitro, BAP, Sucrose

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