Article Per Year (5 Year)
p-Index From 2019 - 2024
5.261
P-Index
This Author published in this journals
All Journal International Journal of Public Health Science (IJPHS) Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu Dasar Jurnal Kedokteran Syiah Kuala Majalah Kedokteran Bandung e-Journal Pustaka Kesehatan Jurnal Kesehatan Masyarakat Indonesian Journal of Tropical and Infectious Disease Jurnal Ilmu dan Teknologi Peternakan Tropis Journal of Agromedicine and Medical Sciences JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Elkawnie MIX : Jurnal Ilmiah Manajemen Majalah Kedokteran Sriwijaya Universa Medicina Paediatrica Indonesiana Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery) Journal Of Vocational Health Studies Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan NurseLine Journal Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan Jurnal Kesehatan Prima JPSCR : Journal of Pharmaceutical Science and Clinical Research Indonesian Journal for Health Sciences (IJHS) SRIWIJAYA JOURNAL OF MEDICINE Journal of Health Sciences JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Public Health and Preventive Medicine Archive Jember Medical Journal (JMJ)
Dini Agustina
Departemen Mikrobiologi Fakultas Kedokteran Universitas Jember, Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

Published : 48 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 48 Documents
Search

 1   2   3   4   5 
PERAN PROTEIN PILI 11 kDa Streptococcus pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Mufida, Diana Chusna; Salsabila, Yuna Annisa; Suswati, Enny; Hermansyah, Bagus; Agustina, Dini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3930

Abstract

Role of Pili Protein 11 kDa of Streptococcus pneumoniae as Hemagglutinin and Adhesin Protein Streptococcus pneumoniae has pili which play roles in adhesion, colonization of nasopharyngeal epithelial cells, and phagocytic inhibition of immune cells. This study aimed to determine the characteristics of the 11 kDa pili protein as hemagglutinin and adhesin, as well as their immune responses. The 11 kDa pili protein from S. pneumoniae was isolated by SDS-PAGE, purified by electroelution and dialysis. Hemagglutination and adhesion tests were carried out on the protein, and western blotting of the polyclonal antibody immune responses were evaluated. Hemagglutination test showed that the 11 kDa pili protein played a role in the hemagglutination process up to 2-time dilution. Adhesion test showed there was a correlation between the dose of the protein and the bacteria attached to the epithelial cells. The Pearson correlation test showed a P value of 0.010 and a correlation coefficient of R = -90.919. Quadratic regression test produced R2 = 0.974. Western blotting test showed that 11 kDa pili protein polyclonal antibodies recognized 67 kDa and 11 kDa pili proteins. The study concluded that the 11 kDa S. pneumoniae pili protein acted as hemagglutinin and adhesin, and the polyclonal antibody protein responded to 67 pDa and 11 kDa BM pili proteins.Keywords: adhesin, hemagglutinin, pili, protein 11 kDa, Streptococcus pneumoniae ABSTRAKStreptococcus pneumoniae memiliki pili yang berperan dalam adhesi, kolonisasi sel epitel nasofaring, serta sebagai inhibitor fagositosis sel imun. Penelitian ini bertujuan mengetahui karakteristik protein pili 11 kDa sebagai hemagglutinin dan adhesin serta respons imunnya. Protein pili 11 kDa dari bakteri S. pneumoniae diisolasi secara SDS-PAGE, dipurifikasi dengan elektroelusi dan dialysis. Uji hemaglutinasi dan adhesi dilakukan pada protein tersebut, serta dievaluasi respon imun poliklonal antibodinya secara western blotting. Uji hemaglutinasi menunjukkan protein pili 11 kDa berperan dalam proses hemaglutinasi hingga pengenceran 2 kali. Uji adhesi menunjukkan korelasi antara dosis protein dan bakteri yang menempel pada sel epitel. Uji korelasi Pearson menunjukkan P value 0,010 dan koefisien korelasi R = -0,919. Uji regresi Quadratic menghasilkan R2 = 0,974. Uji Western blotting menunjukkan antibodi poliklonal protein pili 11 kDa mengenali protein pili 67 kDa dan 11 kDa. Penelitian ini berkesimpulan protein pili 11 kDa S. pneumoniae berperan sebagai hemaglutinin dan adhesin, serta antibodi poliklonal protein tersebut memberi respons terhadap protein pili BM 67 kDa dan 11 kDa. 
Inhibition of Klebsiella pneumoniae adhesion in mice enterocytes by antibodies of hemagglutinin pili protein with MW 12.8 kDa of Klebsiella pneumoniae Agustina, Dini; Retoprawiro, Sumarno; AS, Noorhamdani
Journal of Tropical Life Science Vol 4, No 1 (2014)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Klebsiella pneumoniae as one of the most common cause of VAP is also the second most common cause of community- and hospital-acquired gram negative bloodstream infection. The process of infection caused by direct contact with infectious agents begins with the host cell adhesion process either by pili or by afimbria adhesin (AFA). There were no reported researches on the hemagglutinin pili protein of K.pneumoniae as adhesion factors.This study was conducted in order to determine the hemagglutinin pili protein of K.pneumoniae, polyclonal antibody produced from pili protein immunization, and its ability to inhibit K.pneumoniae adhesion in mice enterocytes. Adhesion inhibition test used HA antibody with the implementation of dose dilutions of 1/100, 1/200, 1/400, 1/800, 1/1600, 1/3200 and 0 (control). While immunocytochemistry test used HA pili protein with the implementation of dose dilutions of 1/10000, 1/20000, 1/40000, 1/80000, 1/160000, 1/320000 and 0 (control). Hemagglutinin pili protein found in K.pneumoniae had MW 12.8 kDa. Pearson correlation analysis of adhesion test showed there were significant correlation between antibody dilution titer with bacterial adhesion (p = 0.032, R = -0.797). Furthermore, Anova analysis of IT showed that there were significant differences between the various dilution titer with antigen-antibody reaction (p=0.000). Antibody of hemagglutinin pili protein with MW 12.8 kDa of K.pneumoniae can inhibit the adhesion of K.pneumoniae to the enterocytes of mice.
MODULASI AKTIVITAS CIPROFLOXACIN TERHADAP Pseudomonas aeruginosa OLEH N-ASETILSISTEIN DAN VITAMIN C Agustina, Dini; Indreswari, Laksmi; Tristianti, Farmitalia Nisa; El Milla, Kardiana Izza; Hermansyah, Bagus; Wahyudi, Septa Surya; Firdaus, Jauhar
Syifa'Medika Vol 11, No 1 (2020): Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan
Publisher : Faculty of Medicine

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32502/sm.v11i1.2389

Abstract

Pseudomonas aeruginosa merupakan salah satu bakteri penyebab infeksi yang sulit diobati dengan terapi obat tunggal karena tingkat keberhasilan yang rendah serta kecenderungan menjadi resisten selama pemberian obat tunggal. Salah satu antibiotik yang digunakan adalah ciprofloxacin. Untuk meningkatkan sensitivitas P. aeruginosa terhadap ciprofloxacin, kombinasi N-asetilsistein atau vitamin C mungkin diperlukan. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi antara N- asetilsistein dan ciprofloxacin serta antara vitamin C dan ciprofloxacin terhadap pertumbuhan P. aeruginosa. Penelitian ini menggunakan uji difusi cakram yang berisi kombinasi konsentrasi ciprofloxacin konstan 1mg/ml dengan N-asetilsistein dalam berbagai konsentrasi 1,25mg/ml, 2,5mg/ml, 5mg/ml, 10mg/ml, dan 20mg/ml dan vitamin C dengan 2,5 mg/ml; 5 mg/ml; 10 mg/ml; 20 mg/ml; dan konsentrasi 40 mg/ml. Dalam kombinasi antara N-asetilsistein dan ciprofloxacin, peningkatan sensitivitas P. aeruginosa terjadi pada konsentrasi 10ml/mg pada kombinasi obat, dan dalam kombinasi antara vitamin C dan ciprofloxacin, peningkatan sensitivitas P. aeruginosa terjadi pada konsentrasi 2, 5 ml/mg pada kombinasi obat di atas kontrol positif. Regresi logaritmik mengungkapkan konsentrasi minimal N- asetilsistein dan vitamin C masing-masing adalah 9,593 mg/ml dan 1,9 mg/ml dapat meningkatkan sensitivitas P. aeruginosa pada ciprofloxacin. Dapat disimpulkan bahwa N-asetilsistein dan vitamin C meningkatkan aktivitas ciprofloxacin untuk menghambat pertumbuhan P. aeruginosa in vitro.
Hubungan antara Higiene Perorangan dan Kejadian Koinfeksi Cacingan pada Penderita Tuberkulosis Shofia, Lailatis; Hermansyah, Bagus; Suswati, Enny; Agustina, Dini; Mufida, Diana Chusna; Shodikin, Muhammad Ali
Sriwijaya Journal of Medicine Vol. 4 No. 1 (2021): Sriwijaya Journal of Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/SJM.v4i1.153

Abstract

Tuberculosis (TB) and intestinal worms are infectious diseases with a large number of sufferers in Indonesia. The high prevalence of intestinal worms in Indonesia allows the occurrence of STH coinfection in pulmonary TB patients which causes anti M. tuberculosis immunity to decrease so that the response to tuberculosis treatment is not optimal. One of the risk factors of STH infection is personal hygiene. The purpose of this study was to analyze the relationship between individual hygiene and the incidence of STH coinfection in TB patients in Tempurejo Sub-District, Jember District. This type of research is observational research with cross sectional analytic design. The population used was TB patients in Tempurejo Sub-District in the period September - October 2019. The data used in this study were primary data in the form of interviews and results of stool examination. Stool examination is carried out using the sedimentation and floatation methods. Data analysis was performed using Fisher's exact test. The results of this study indicate that the incidence of STH co-infection in TB patients in Tempurejo Subdistrict is 9.67% and is caused by two STH species, A. lumbricoides (66.7%) and Hookworm (33.3%). Respondents' personal hygiene consisted of good hygiene (64.5%) and bad hygiene (35.5%), where 66.7% of cases of STH coinfection occurred in respondents with poor personal hygiene. Fisher's test results showed that there was no significant relationship between personal hygiene with the incidence of STH coinfection in TB patients in Tempurejo Sub-District, Jember District (p-value of 0.281).
Environmental Sanitation, Personal Hygiene, STH Co-infection in TB Patients Fitri, Nadya Eka; Mufida, Diana Chusna; Hermansyah, Bagus; Armiyanti, Yunita; Agustina, Dini; Suswanti, Enny
KEMAS: Jurnal Kesehatan Masyarakat Vol 16, No 3 (2021)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v16i3.23639

Abstract

Globally, helminthiasis is one of the infectious diseases that are often associated with the incidence of tuberculosis. Helminth co-infection modulates the immune system of TB patients by reducing Th-1 response that functions as protector against Mycobacterium tuberculosis. This condition disrupts the process of eliminating bacteria so that its development becomes more progressive. The impact is the activation of latent TB and the success of TB treatment. A Soil-transmitted Helminth (STH) is a group of intestinal worms that often infect humans. Previous studies prove that environmental sanitation and personal hygiene are risk factors associated with STH infection. The study to determine the relationship of environmental sanitation and personal hygiene with the incidence of STH co-infection in TB patients at Puskesmas Puger, Jember in September until December 2019. This study uses a cross-sectional design. The sampling technique uses total sampling. A total of 32 TB patients met the criteria as study sample. The data analysis uses Fisher Test. Results showed the prevalence of STH co-infection was relatively low (18.8%); most of the respondents had good environmental sanitation and personal hygiene conditions. Bivariate analysis showed p-value (0,476) for environmental sanitation and p-value (1,000) for personal hygiene. This study concludes that there is no correlation between environmental sanitation, personal hygiene, and STH co-infection in TB patients at Public health center in Puger.
Interleukin-22-induced β‑defensin-2 expression by intranasal immunization with Streptococcus pneumoniae RrgB epitopes Mufida, Diana Chusna; Saputra, Antonius Dwi; Hermansyah, Bagus; Agustina, Dini; Shodikin, Muhammad Ali; Armiyanti, Yunita
Universa Medicina Vol. 41 No. 1 (2022)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2022.v41.37-46

Abstract

BackgroundStreptococcus pneumoniae causes pneumococcal disease, which is responsible for millions of deaths worldwide. Various pneumococcal vaccine candidates have been developed to prevent S. pneumoniae infection, one of which is an epitope-based vaccine. This study aimed to prove that intranasal immunization with each of the five S. pneumoniae RrgB epitopes can induce a mucosal immune response by increasing the β-defensin-2 concentration through upregulation of interleukin (IL)-22 expression. MethodsAn experimental laboratory study was conducted using 28 male Wistar rats aged 3-4 months, that were randomly divided into 7 groups containing four rats each. Group 1 was given 40 mL of phosphate-buffered saline (PBS) only (control group). Group 2 was the adjuvant group that received 40 mL PBS containing 2 ìg cholera toxin B (CTB), and groups 3-7 were immunized with 40 mL PBS containing a combination of adjuvant and one of the five different S. pneumoniae RrgB epitopes. The concentrations of IL-22 and β-defensin-2 from nasal rinse examination were measured by means of ELISA. The Kruskal-Wallis test, followed by the Mann-Whitney post-hoc test were used for statistical analysis. ResultsRats immunized with the adjuvant-epitope combination had significantly higher β-defensin-2 and IL-22 levels than the control group (p=0.030; p=0.018, respectively), according to the Kruskal-Wallis test. And the Mann-Whitney statistical test, showed there was a significant increase in β-defensin-2 and IL-22 levels. ConclusionsIntranasal immunization with epitope 1 of the S. pneumoniae RrgB can increase β-defensin-2 expression significantly and has a greater potential to be developed into a pneumococcal vaccine.
Parasite immunomodulatory role in reducing the prevalence of COVID-19 in endemic regions Hermansyah, Bagus; Agustina, Dini; Zulaikha, Siti; Habibi, Ali
Universa Medicina Vol. 41 No. 1 (2022)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2022.v41.90-99

Abstract

Nearly 35 million cases and one million deaths over the nine months of the COVID-19 pandemic have been reported worldwide. Africa and some countries with endemic parasitic infections had a low incidence of COVID-19. By contrast, the United States and several European countries, having a non-endemicity of parasitic infections, recorded a high incidence of COVID-19. Some parasites have an immunomodulatory mechanism that can induce an immune tolerance state in the infected persons by balancing pro-inflammatory and anti-inflammatory responses. Emerging reports also stated that COVID-19 and helminth co-infections may have more hidden outcomes than predictable ones. Hence, the aim of this literature review is to show and identify that an increase in the number of regulatory immune cells due to the immunomodulatory role of a pre-existing parasitic infection could reduce the risk of COVID-19. This study explored the existing literature to determine the role of parasitic infections in modulating the immune response and possibly reduce the risk of COVID-19 infection in endemic countries. The mechanism of immunomodulation by parasites is the increased numbers of Treg cells, M2 macrophages, eosinophils, the Th2 cytokines IL-4 and IL-5, and the pro-inflammatory downregulation of IFN λ, TNF α, and IL-6, which play an essential role in inducing cytokine storms in COVID-19 infection. This condition will probably occur in an individual with parasitic infection in a community with limited facilities and infrastructure to treat parasitic infections, particularly in developing countries. To conclude, in endemic areas, the immunomodulatory effect of parasitic infection to reduce the risk of COVID-19 cases/deaths is a possibility if the host is immunocompetent. Herein, the current knowledge on the immunomodulatory role of COVID-19 and helminth co-infections will be discussed.
PERAN PROTEIN PILI 11 kDa Streptococcus pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Diana Chusna Mufida; Yuna Annisa Salsabila; Enny Suswati; Bagus Hermansyah; Dini Agustina
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3930

Abstract

Role of Pili Protein 11 kDa of Streptococcus pneumoniae as Hemagglutinin and Adhesin Protein Streptococcus pneumoniae has pili which play roles in adhesion, colonization of nasopharyngeal epithelial cells, and phagocytic inhibition of immune cells. This study aimed to determine the characteristics of the 11 kDa pili protein as hemagglutinin and adhesin, as well as their immune responses. The 11 kDa pili protein from S. pneumoniae was isolated by SDS-PAGE, purified by electroelution and dialysis. Hemagglutination and adhesion tests were carried out on the protein, and western blotting of the polyclonal antibody immune responses were evaluated. Hemagglutination test showed that the 11 kDa pili protein played a role in the hemagglutination process up to 2-time dilution. Adhesion test showed there was a correlation between the dose of the protein and the bacteria attached to the epithelial cells. The Pearson correlation test showed a P value of 0.010 and a correlation coefficient of R = -90.919. Quadratic regression test produced R2 = 0.974. Western blotting test showed that 11 kDa pili protein polyclonal antibodies recognized 67 kDa and 11 kDa pili proteins. The study concluded that the 11 kDa S. pneumoniae pili protein acted as hemagglutinin and adhesin, and the polyclonal antibody protein responded to 67 pDa and 11 kDa BM pili proteins.Keywords: adhesin, hemagglutinin, pili, protein 11 kDa, Streptococcus pneumoniae ABSTRAKStreptococcus pneumoniae memiliki pili yang berperan dalam adhesi, kolonisasi sel epitel nasofaring, serta sebagai inhibitor fagositosis sel imun. Penelitian ini bertujuan mengetahui karakteristik protein pili 11 kDa sebagai hemagglutinin dan adhesin serta respons imunnya. Protein pili 11 kDa dari bakteri S. pneumoniae diisolasi secara SDS-PAGE, dipurifikasi dengan elektroelusi dan dialysis. Uji hemaglutinasi dan adhesi dilakukan pada protein tersebut, serta dievaluasi respon imun poliklonal antibodinya secara western blotting. Uji hemaglutinasi menunjukkan protein pili 11 kDa berperan dalam proses hemaglutinasi hingga pengenceran 2 kali. Uji adhesi menunjukkan korelasi antara dosis protein dan bakteri yang menempel pada sel epitel. Uji korelasi Pearson menunjukkan P value 0,010 dan koefisien korelasi R = -0,919. Uji regresi Quadratic menghasilkan R2 = 0,974. Uji Western blotting menunjukkan antibodi poliklonal protein pili 11 kDa mengenali protein pili 67 kDa dan 11 kDa. Penelitian ini berkesimpulan protein pili 11 kDa S. pneumoniae berperan sebagai hemaglutinin dan adhesin, serta antibodi poliklonal protein tersebut memberi respons terhadap protein pili BM 67 kDa dan 11 kDa. 
HUBUNGAN KEPADATAN DERMATOPHAGOIDES SPP. DENGAN FREKUENSI SERANGAN ASMA PADA PENDERITA ASMA DI RS PARU JEMBER Denaneer Rahmadatu; Erma Sulistyaningsih; Dini Agustina
Jurnal Kedokteran Syiah Kuala Vol 19, No 2 (2019): Volume 19 Nomor 2 Agustus 2019
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jks.v19i2.18058

Abstract

Abstrak. Asma merupakan gangguan inflamasi kronik saluran napas yang bersifat hiperesponsif yang ditandai adanya gejala episodik berulang berupa mengi, sesak napas, dada terasa berat, dan batuk, terutama pada malam hari atau dini hari. Sekitar 50-80% asma disebabkan oleh tungau debu rumah (TDR), khususnya Dermatophagoides pteronyssinus dan Dermatophagoides farinae. Penelitian ini bertujuan untuk mengetahui apakah terdapat hubungan antara kepadatan Dermatophagoides spp. pada debu rumah dengan frekuensi serangan asma pada penderita asma di RS Paru Jember. Penelitian ini merupakan penelitian analitik observasional dengan desain penelitian cross-sectional. Sampel penelitian ini yaitu 30 penderita asma di RS Paru Jember yang diambil menggunakan teknik simple random sampling sesuai dengan kriteria inklusi dan eksklusi. Peneliti melakukan interview mengenai frekuensi serangan asma dalam tiga bulan terakhir, mengambil debu dari ruang tamu dan ruang tidur pada rumah penderita asma, dan mengidentifikasi tungau debu rumah (TDR) yang ditemukan pada debu. Hasil uji Pearson mendapatkan nilai p=0,011 dan nilai r=0,475 sehingga dapat disimpulkan bahwa terdapat hubungan bermakna antara kepadatan Dermatophagoides spp. dengan frekuensi serangan asma dengan kekuatan korelasi sedang pada penderita asma di RS Paru Jember. Kata Kunci: Dermatophagoides spp., Tungau Debu Rumah (TDR), Asma Abstract. Asthma is a chronic inflammatory disorder of the respiratory system characterized by recurrent episodes of wheezing, shortness of breath, chest tightness, and cough, particularly at night or early morning. About 50-80% of asthma is caused by house dust mite (HDM), especially Dermatophagoides pteronyssinus and Dermatophagoides farinae. This study aimed to investigate the correlation between the density of Dermatophagoides spp. and the frequency of asthma attacks in asthmatic patients at Jember Pulmonary Hospital. This study was an observational analytic study with a cross-sectional design. Respondents were 30 asthmatic patients at Jember Pulmonary Hospital matched with inclusion and exclusion criteria and chose by simple random sampling. Respondents were interviewed about the frequency of asthma attacks in the last three month,s and the hose dust from the living and sleeping rooms were collected for identification of house dust mites (HDM). The pearson test showed a value of p=0.011 and r=0.475. It can be concluded that there is a significant correlation with the moderate strenght between the density of Dermatophagoides spp. and the frequency of asthma attacks in the asthmatic patient at Jember Pulmonary Hospital. Keywords: Dermatophagoides spp., House Dust Mites (HDM), Asthma
Prevalensi Resistensi Escherichia Coli Terhadap Tetrasiklin yang Diisolasi dari Hati Ayam Broiler Siti Ananda Hardita Syahputri; Enny Suswati; Yudha Nurdian; Supangat Supangat; Dini Agustina
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 8, No 3 (2021): JITRO, September 2021
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.333 KB) | DOI: 10.33772/jitro.v8i3.17584

Abstract

ABSTRAK Antibiotik berguna untuk kesehatan manusia maupun hewan. Tetrasiklin salah satu jenis antibiotiik yang paling sering digunakan di peternakan ayam untuk tujuan pengobatan, pencegahan maupun perangsang pertumbuhan (Antibiotic Growth promoter/AGP) dalam pakan ayam broiler. Bakteri Escherichia coli (E. coli) banyak ditemukan di lingkungan peternakan dan dapat mencemari hati ayam. Tujuan dari penelitian ini untuk mengetahui resistensi bakteri E. coli yang diisolasi dari hati ayam broiler terhadap tetrasiklin. Penelitian ini merupakan penelitian deskriptif. Sampel yang digunakan bakteri E. coli pada hati ayam broiler. Ayam broiler diambil berasal dari PT X yang berada pada 6 desa di Kecamatan Sumbersari, Kabupaten Jember. Uji resistensi bakteri dilakukan dengan Metode Kirby Bauer. Data dianalisis dengan metode Clinical and Laboratory Standards Institute. Hasil penelitian ini menunjukkan bahwa bakteri yang ditemukan dari hati ayam 100% adalah golongan bakteri Gram negatif. Berdasarkan hasil kultu rpada media EMB dan pengecatan Gram sebanyak 67% sampel teridentifikasi positif bakteri E. coli. Pada uji resistensi antibiotik didapatkan 50% sensitif, 25% intermediet dan 25% resistensi terhadap tetrasiklin. Kesimpulan pada penelitian ini bakteri E. coli yang diisolasi dari hati ayam broiler 25% resisten terhadap tetrasiklinKata kunci: Eschecicia coli, tetrasiklin, hati ayam, resistancePrevalence of Escherichia Coli Resistance to Tetracyclin Isolated from Broiler Chicken LiverABSTRACT Antibiotics are useful for human and animal health. Tetracyclines are one of the most commonly used antibiotics in chicken farms for medicinal purposes, prevention, and growth stimulants (Antibiotic Growth promoter / AGP) in broiler feed. Escherichia coli (E. coli) bacteria are found in many livestock environments and can contaminate chicken livers. The purpose of this study was to determine the resistance of E. coli isolated from broiler chicken livers to tetracyclines. This research is descriptive. The samples used were E. coli bacteria in the broiler liver. Broiler chickens were taken from PT X in 6 villages in Sumbersari District, Jember Regency. The test for bacterial resistance was carried out using the Kirby Bauer method. Data were analyzed using the Clinical and Laboratory Standards Institute method. The results of this study indicated that the bacteria found from 100% chicken liver were gram-negative bacteria. Based on the results of culture on EMB media and Gram staining, 67% of the samples were identified as positive for E. coli bacteria. In the antibiotic resistance test, it was found that 50% sensitivity, 25% intermediates, and 25% resistance to tetracyclines were found. The conclusion in this study was that E. coli bacteria isolated from 25% broiler chicken liver was resistant to tetracyclines.Keywords: Eschecicia coli, tetracyclines, chicken liver, resistance
Co-Authors Aditya Primadana Ali Santosa Anaditya Wahyu Kumudhaningsih Anastasia Citra Purwani Angga Mardro Raharjo Astuti Setyawardani Bagus Dwi Kurniawan Bagus Dwi Kurniawan Bagus Hermansyah Bagus Hermansyah Bagus Hermansyah Bima Setia Sandya Nugraha Budi Suharjo Cholis Abrori, Cholis Cicih Komariah Cicih Komariah Denaneer Rahmadatu Desie Dwi Wisudanti Diana C. Mufida Diana Chusna Mufida Diana Chusna Mufida Dion Krismashogi Dharmawan Dwita Aryadina Rachmawati El Milla, Kardiana Izza Ellen Ocktavironita Elly N Sakinah Elvia Rahmi Marga Putri Endiningtyas Cahyaningrum Enny Suswanti Enny Suswati ENNY SUSWATI Erfan Efendi Erma Sulistyaningsih Fitri, Nadya Eka Gama Wisnu Sanjaya Habib Mustofa Habibbur Rochman Salim Habibi, Ali Hamzah, Salsabilla Maula Zalfa El Hanifa Rizki A.S. I Ketut Sudiana I Nyoman Semita I Nyoman Semita Ika Rahmawati Sutejo Intan Palupi Irawan Fajar Kusuma Jauhar Firdaus Kirana Nadyatara Kristianningrum Dian Sofiana Laila Rizqi Kurniawati Lailatis Shofia Laksmi Indreswari M. Ali Shodikin Mei Liana Wati Miftakhul Huda Muhamad Dwi Eka Putra Muhammad A. Shodikin Muhammad Ali Shodikin Muhammad Dimas Arya Candra Permana Mukhamad Najib Nastiti Bekti Utami Nihayah Lukman Nisrina Salsabila Firmansyah Nita Alfianti Noorhamdani AS Noorhamdani AS Nurul Indah Saffanah Nurul Indah Saffanah Nurus Saffana Yulianto Pralampita, Pulong Wijang Regina Finka Rinda Yanuarisa Rony Prasetyo Ryan Ravi Is Syahputra Salsabila, Yuna Annisa Salsabilla Maula Zalfa El Hamzah Saputra, Antonius Dwi Sarah Marsa Tamimi Semita, I Nyoman septa surya wahyudi, septa surya Setyawardani, Astuti Shofia, Lailatis Siti Ananda Hardita Syahputri Siti Marissa Aisyah Siti Zulaikha Sumarno Retoprawiro Supangat Supangat Tristianti, Farmitalia Nisa Ulfa Elfiah Ulin Nuha Jazmin Widhiasari Normaningtyas Wiwien Sugih Utami Wiwien Sugih Utami Wiwien Sugih Utami Yudha Ananta Khaerul Putra Yudha Nurdian Yuli Hermansyah Yuna Annisa Salsabila Yunita Armiyanti Yunita Armiyanti