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All Journal Bioscience Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis FONDATIA AL-ULUM: JURNAL SAINS DAN TEKNOLOGI Atrium Pendidikan Biologi Journal On Teacher Education (Jote)

Irdawati Irdawati

Jurusan Biologi FMIPA Universitas Negeri Padang

Author-ID : 2543053

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Education Energy Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Physics Social Sciences Other

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Kombucha Tea Production Using Different Tea Raw Materials Putri Ardila Sari; Irdawati Irdawati
Bioscience Vol 3, No 2 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Tea (Camellia sinensis) is an agricultural product that is widely issued by people around the world. Some types of tea in the world are green tea, black tea, oolong tea and white tea. Kombucha tea is brewed water for tea and sugar then given bacteria and fungi and fermented for 7-10 days. The purpose of this study was to determine the quality of kombucha of several different types and determine the organoleptic value of fermented kombucha tea using different types. This research was conducted in April-July 2019 in the Microbiology Laboratory of the Department of Biology FMIPA Padang State University. This research is an experimental study with 5 treatments and 1 repetition (K: kombucha starter, P1: kombucha starter + green tea, P2: kombucha starter + black tea, P3: kombucha starter + oolong tea, P4: kombucha starter + white tea. Parameters Calculated are the number of microbes, nata thickness, pH of kombucha tea, total acetic acid, and organoleptic tests of kombucha tea.The results of this study indicate differences in the types of tea used in kombucha fermentation, the quality of kombucha tea produced, kombucha tea using black tea. The organoleptic test requested by the panelists was kombucha tea from white tea.

The Thermophilic Bacterial Growth Curve Irdawati Irdawati; Ilsa Septia Putri; Syamsuardi Syamsuardi; Anthoni Agustien; Yetria Rilda
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Thermophilic bacteria are bacteria that can produce thermostable enzymes and used in various industries. Thermostable enzymes that can be produced by thermophilic microorganisms one of them is xylanase enzyme. Xylanase produced by microbes has optimum temperature characteristics and more diverse optimum pH on various substrates, which will affect the activity of the resulting xylanase enzyme. The influence of temperature greatly determines the activity of the enzyme at the time of catalyzing a reaction. At optimum pH conditions, the enzyme has an active side conformation that is substrate-like so that it can form a complex of appropriate enzymes and produce the product to its full potential. One of the thermophilic bacterial habitats of hot springs in West Sumatera is the Aro Sapan River hot spring located in Koto Parik Gadang Subdistrict in Ateh, South Solok District. The Saw Aro River hot spring has a temperature of 75 ° C and is pH 8 or alkaline. The aim to this research was to know the profile of the growth of thermophilic bacteria. This research is a descriptive method. The data obtained were analyzed descriptively. The conclusion is the best activity of xylanase thermophilic bacteria was at the sixth time incubation.

Isolation and Activity Test of Antimicrobial Endophytic Bacteria from Leaf Salam (Syzygium polyanthum Wight) Irdawati Yahya; Linda Advinda; Fitri Angraini
Bioscience Vol 1, No 2 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Abstract -- Endophytic bacteria are microorganisms that exist in the system of plant tissues such as leaves, twigs, roots and they can form colonies without causing damage to the plant. The endophytic bacteria may produce secondary metabolites in accordance. One potential medicinal plant has endophytic bacteria are plant greeting (Syzygium polyanthum Wight). Part of the plant Salam particularly frequently used is part of the leaf, which serves as an antibacterial and antifungal. Because the leaves contain compounds bay leaf tannins, flavonoids and essential oils. The porpuse of this research was to find of to obtain isolates of endophytic bacteria and determine the antimicrobial activity of bay leaf (Syzygium polyanthum Wight).This research is descriptive that has done on January - February 2015 of Microbiology Laboratory at Biology Departement FMIPA, UNP. The parameters observed in the determination of isolates is a form colonies, colonies edge, elevation colonies, colony color, and Gram staining of leaves (Syzygium polyanthum Wight). The antimicrobial activity was observed with the formation zone of inhibition around the paper disc. Obtained were 11 isolates of endophytic bacteria from leaves, 5 isolates of endophytic bacteria have the ability to produce antimicrobial.Isolates had the highest antimicrobial activity that isolates DSA 1-1 with a 6.42 mm diameter inhibition zone. Keywords: antimicrobial, endophytic bacteria, bay leaf.

Identification of fungi in biogas mixed with buffalo dung and leaf onion waste (Allium cepa L.) Mei Niarti; Irdawati Irdawati; Moralita Chatri; Des M Des
Bioscience Vol 5, No 2 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202152106831-0-00

Biogas is a gas produced from the decomposition process of organic materials by microorganisms in anaerobic conditions. Waste of red onion (Allium cepa L.) and buffalo dung contain organic substances and nutrients that can be used as biogas. The purpose of this study was to identify fungi in biogas mixed with buffalo dung with scallion waste (Allium cepa L.).The study was conducted from April to September 2019 in the Microbiology Laboratory and Integrated Research Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, State University. This research is a descriptive study by observing macroscopic and microscopic characters as a basis for identification produced by fungi in buffalo dung biogas (Pariaman, West Sumatra) and onion leaf waste from Alahan Panjang, West Sumatra.The results of this study obtained 5 genus of fungi on biogas substrate from a mixture of buffalo dung with scallion waste (Allium cepa L.) including: Aspergillus, Trichoderma, Candida, Mucor and Humicolla. Biogas adalah gas yang dihasilkan dari proses penguraian bahan-bahan organik oleh mikroorganisme dalam keadaan anaerob. Limbah daun bawang merah (Allium cepa L.) dan kotoran kerbau mengandung zat organik dan unsur hara yang dapat dijadikan sebagai biogas. Tujuan penelitian ini untuk mengidentifikasi jamur pada biogas campuran kotoran kerbau dengan limbah daun bawang merah (Allium cepa L.). Penelitian dilaksanakan dari bulan April sampai bulan September 2019 di Laboratorium Mikrobiologi dan Laboratorium Penelitian Terpadu Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri. Penelitian ini merupakan penelitian deskriptif dengan mengamati karakter makroskopis dan mikroskopis sebagai dasar identifikasi yang dihasilkan oleh jamur pada biogas kotoran kerbau (Pariaman, sumatera barat ) dan limbah daun bawang merah dari alahan panjang, sumatera barat. Hasil dari penelitian ini diperoleh 5 genus jamur pada substrat biogas dari campuran kotoran kerbau dengan limbah daun bawang merah (Allium cepa L.) diantaranya yaitu: Aspergillus, Trichoderma, Candida, Mucor dan Humicolla.

Enumertion of Enterobacteriaceae in Ice-Based School Children Food in Koto Tangah District, Padang West Sumatera Bob Refianto; Irdawati Irdawati
Bioscience Vol 4, No 1 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Elementary students have high playing activities while in school. Hot weather and feeling tired after playing, will cause thirst and to get rid of thirst Elementary school students will buy ice-based snacks. They do not realize, the danger of unhygienic snacks will cause diarrheal diseases, because ice cubes are made by home industries still traditionally without considering hygiene requirements. In some countries, the food industry has moved towards testing Enterobacteriaceae which shows enteric bacterial contamination as a condition hygiene. This study aimed to determine the microbiological hygiene quality of the Enterobacteriaceae numbers in ice-based school children snacks in Koto Tangah Subdistrict, Padang City. This study was a descriptive study. The study was conducted from April to May 2019 at the Laboratory of Microbiology at the Center for Drug and Food Control in Padang. Total samples taken were 20 samples. The results showed that the sample that fulfilled the microbiological quality requirements for sadness was 60% (12 samples) and 40% (8 samples) that did not meet the requirements, the highest results were obtained at SDN 44 Sungai Lareh with a value of 9.82 x 105 colonies/mL.

Optimization of Medium Fermentation for Production of Antimicrobial Compounds by Endofit Bacteria Andalas Plant (Morus macroura Miq.) B.J.T.A-6 Isolate Nada Nafion; Dwi Hilda Putri; Irdawati Irdawati
Bioscience Vol 3, No 1 (2019): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Cases of bacterial resistance to antibiotics are discussed with serious problems in the world of health. New antimicrobial compounds are needed which are more effective in treating infectious diseases. Isolate B.J.T.A-6 is an endophytic bacteria from Andalas plants (Morus macroura Miq.) which is known to be able to produce antimicrobial active compounds. Antimicrobial compounds can be produced by growing them on fermented media. The purpose of this study was to optimize the medium fermentation of Andalas endophytic bacteria of B.J.T.A-6 isolates in producing antimicrobial compounds. While medium fermentation is Nutrient Broth (NB), Muller Hinton (MH), and Luria Bertani Broth (LB). Antimicrobial activity tests were carried out by means of diffusion. The parameters used were the diameter of the inhibition zone against Staphylococcus aureus around the disc paper. The profile of medium fermentation optimization was analyzed statistically. The results showed that B.J.T.A-6 isolates could inhibit the highest growth of S. aureus by using LB fermentation medium.

Isolation and Identification of Endophytic Bacteria from the Andalas Plant Stem (Morus macroura Miq.) Nisa Afifah; Dwi Hilda Putri; Irdawati Irdawati
Bioscience Vol 2, No 1 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

The number of death couse of infection continues to increase. Treatment of infections is increasingly difficult due to increased resistance of germs to existing antimicrobial compounds. A new source of antimicrobial substances is needed. One source of antimicrobial substances is endophytic bacteria that live in the stems of Andalas plants. The aim of this study was to compare the diversity of endophytic bacteria present in old and young Andalas plant stems. The method used to isolate endophytic bacteria from the stem of the Andalas plant is the streak plate method. Bacterial identification is done macroscopically and microscopically. The results of macroscopic observation showed that there are differences in the number of isolates found in old and young Andalas plant stems. 11 isolates of endophytic bacteria from Andalas plant stems, each of which consists of 9 endophytic bacteria from old Andalas stems and two isolates from young Andalas stems. A microscopic identification was found that of the 11 isolates isolated, eight isolates were gram-positive and three isolates were gram-negative bacteria

Antibacterial Activities of Kombucha Tea From Some Types of Variations of Tea on Escherichia coli and Staphylococcus aureus Witri Nofita Safitri; Irdawati Irdawati
Bioscience Vol 4, No 2 (2020): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202042105679-0-00

Tea can be used as a fermented drink called kombucha tea. Based on the oxidation process tea is classified into white tea, green tea, oolong tea and black tea. The compounds produced from the fermentation of kombucha tea have a fairly good antibacterial effect. This study aims to determine the antibacterial activity of kombucha tea fermentation from several variations of tea types against Escherichia coli and Staphylococcus aureus. This research is an experimental study with a completely randomized design (CRD) with 5 treatments and 3 replications conducted in May-June 2019 at the Microbiology Laboratory and the Integrated Research Laboratory of the Department of Biology FMIPA UNP. Kombucha tea used in this study was white tea, green tea, oolong tea and black tea. Antibacterial activity test was carried out by paper disc diffusion method against microbial test of S. aureus and E. coli bacteria. Research results showed that the highest antibacterial activity of kombucha tea fermentation in E.coli was found in black tea with a value of 1.64 cm and the lowest in tea white with a value of 1.04 cm. In S. aureus the highest value was obtained in black tea with a value of 2.48 cm and the lowest in white tea with a value of 1.39 cm.

Isolasi dan Identifikasi Bakteri Halofilik Ikan Talang (Chorinemus sp.) dari Aia Bangih Pasaman Barat Mades Fifendy; Faradilla Rattriana; Irdawati Irdawati
Bioscience Vol 1, No 2 (2017): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Halophilic bacteria is microorganism that live in high salinity environments up to 30%. The bacteria can be found in foods preserved by salting like salted fish. Salted fish is preserved foods are processed by salting and drying. The bacteria that live and ruin salted fish are halophilic bacteria and heterotoleran bacteria like halobacterium, micrococcus, bacillus, pediococcus, peseudomonas and vibrio. This observation aims to determine type of halophilic bacteria found in talang fish. This is descriptif observation held january up to february 2015 is microbiology laboratory FMIPA UNP and balai veteriner laboratory Bukittinggi. Research methods is cup statterplot methods. Sample is talang fis from Aia Bangih, west pasaman. Based on observation found isolate halophilic bacteria. Its belong to gram positve like coccus. From both, the isolates not capable produce endosopra. Talang fish from Aia Bangih, west pasaman contain two isolate halophilic bacteria consist of a genus and two species.Keywords : talang salted fish, halphilic bacteria, biochemical test

Potential of thermophilic bacteria to produce bioethanol at variation of incubation pH Irdawati Irdawati; Nur Vaizi; Linda Advinda; Dwi Hilda Putri
Bioscience Vol 6, No 2 (2022): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202262117426-0-00

The purpose of this study was to determine the potency and optimum pH of AAS thermophilic bacteria isolates in producing bioethanol. This research was carried out from September 2021 to December 2021, at the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Padang State University. This research is an experimental study consisting of 6 treatments and 3 replications with variations in incubation pH of 6.5, 7, 7.5, 8, 8.5, and 9. The data obtained were analyzed by means of variance (ANOVA) with Duncan's further test. New Multiple Range Test (DNMRT). The results showed that the isolates of thermophilic bacteria SSA had the potential to produce bioethanol with isolates SSA 8 being the producer of bioethanol with the highest concentration of 1.001%, and variations in incubation pH affected the concentration of bioethanol produced with the optimum pH of thermophilic bacteria in producing bioethanol, namely pH 8 which produced the highest concentration of bioethanol is 1.001%.Tujuan dari penelitian ini adalah untuk mengetahui potensi serta pH optimum isolat bakteri termofilik SSA dalam menghasilkan bioetanol. Penelitian ini dilaksanakan pada bulan September 2021 hingga bulan Desember 2021, di Laboratorium Mikrobiologi Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Padang. Penelitian ini merupakan penelitian eksperimen yang terdiri dari 6 perlakuan dan 3 ulangan dengan variasi pH inkubasi 6,5, 7, 7,5, 8, 8,5, dan 9. Data yang diperoleh dianalisis dengan sidik ragam (ANOVA) dengan uji lanjut Duncan’s New Multiple Range Test (DNMRT). Hasil penelitian menunjukkan bahwa isolat bakteri termofilik SSA memiliki potensi dalam menghasilkan bioetanol dengan isolat SSA 8 merupakan penghasil bioetanol dengan kadar tertinggi yaitu 1.001%, dan variasi pH inkubasi berpengaruh terhadap konsentrasi bioetanol yang dihasilkan dengan pH optimum bakteri termofilik dalam menghasilkan bioetanol yaitu pH 8 yang menghasilkan konsentrasi bioetanol tertinggi yaitu 1.001%.

Co-Authors Anthoni Agustien Ardi Ardi Azwir Anhar Bob Refianto Des M Des Desi Nurmayenti Dezi Handayani Dwi Hilda Putri Faradilla Rattriana Fitri Angraini Fitri Arsih Ganda Hijrah Selaras Heffi Alberida Ilsa Septia Putri Indrawani Matondang Jannah Koftiah Khalif Alfajri Linda Advinda Linda Advinda Mades Fifendy Mei Niarti Moralita Chatri Moralita Chatri Mulia Mulia Nada Nafion Nisa Afifah Nur Vaizi Putri Ardila Sari Putri Rachma Auliya Putri Sarinami Rizka Putri Alti Sasmeri Sasmeri Syamsuardi Syamsuardi Vauzia Vauzia Witri Nofita Safitri Yetria Rilda Yusrizal Y Yusrizal Yusrizal Zulyusri Zulyusri

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