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Kualitas Makroskopis Semen Ayam Kampung yang Diberi Ekstrak Kulit Buah Naga Dhian Ramadhanty; Angga Nugraha; Nurul Purnomo; Andi Fausiah
AGROVITAL : Jurnal Ilmu Pertanian Vol 6, No 1 (2021): Agrovital Volume 6, Nomor 1, Mei 2021
Publisher : Universitas Al Asyariah Mandar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35329/agrovital.v6i1.2005

Abstract

Motilitas dan Abnormalitas Spermatozoa Ayam Kampung yang Diberi Suplementasi Ekstrak Kulit Buah Naga Dhian Ramadhanty; Angga Nugraha; Nurul Purnomo; Armayani M
JURNAL GALUNG TROPIKA Vol 10 No 1 (2021)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v10i1.703

Abstract

This study aimed to determine the effect of dragon fruit peel extract on the motility and abnormalities of native chicken spermatozoa. The study was arranged experimentally using a completely randomized design consisting of 4 treatments and 3 replications. Before treatment, 12 native chicken were randomly divided into groups and adapted for 10 days with commercial feed and drinking water. The treatments were given orally for 8 weeks and consisted of P0 (control), P1 (1 ml dragon fruit peel extract), P2 (3 ml dragon fruit peel extract), and P3 (5 ml dragon fruit peel extract). Cement storage is carried out by the sequencing method. The quality of the collected sperm is tested in the laboratory. The parameters measured were microscopic sperm quality, namely sperm motility, and abnormalities. The results showed that dragon fruit peel extract had a significant effect on spermatozoa motility by increasing the percentage of motility but had no effect on spermatozoa abnormalities. Provision supplement of 5 ml dragon fruit peel extract gave the best results in increasing spermatozoa motility in native chickens.
KUALITAS DNA HASIL PURIFIKASI DARI SOSIS SAPI SEBAGAI BAHAN AUTENTIKASI HALAL BERBASIS MARKER GENETIK Nurul Purnomo; Dhian Ramadhanty; Musdalifa Mansur; Maghfira Nur; Muhammad Ihsan A. Dagong
Jurnal Ilmu dan Teknologi Peternakan Vol. 9 No. 1 (2021)
Publisher : Fakultas Peternakan, Universitas Hasanuddin, Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jitp.v9i1.11633

Abstract

This study aims to determine the effect of the preparation method and the number of samples on the quality of purified DNA from beef sausage. The study was conducted using a Randomized Block Design. The main factor is the sample preparation method which consists of P1: fresh sample; P2: oven-dried sample; and P3: Freeze-drying dry samples. Each treatment consisted of 4 groups, namely K1: sample weight 25 mg; K2: sample weight 50 mg; K3: sample weight 75 mg; and K4: sample weight 100 mg. Each treatment group was repeated 2 times so that the total sample was 24. The results showed that DNA purification using different preparation methods and sample weights was successfully carried out, as seen from the presence of DNA bands visualized on agarose gel with EtBr staining. The average DNA quality of the P1, P2, and P3 preparation methods were 1.60±0.90 ng/μl, 2.63±0.99 ng/μl, and 2.94 ±0.89, ng/μl respectively, with DNA purity 1.023 ±0.165, 0.937±0.148, and 1.014±0.163. The average DNA quality at K1, K2, K3, and K4 obtained DNA concentrations of 3.03±1.64 ng/μl, 3.15±0.74 ng/μl, 2.28± 1.66 ng/μl, and 2.54±1.50 ng/μl with a purity of 1.059±0.142, 0.981±0.130, 0.908±0.061, and 1.061± 0.215. The average total concentration of purified DNA from beef sausage was 2.75±1.28 ng/μl with a purity of 0.991±0.149. The results of variance showed that the treatment did not affect the concentration and purity of purified DNA from beef sausage. This study concludes that DNA purification from beef sausage can be carried out, but the preparation method and number of samples do not affect the quality and quantity of DNA.
Pengaruh Suplementasi Ekstrak Daun Kelor (Moringa oleifera) Terhadap Kualitas Makroskopis Spermatozoa Ayam Kampung Dhian Ramadhanty; Nurul Purnomo; Andi Fausiah; Dwi Maryana
AGROVITAL : Jurnal Ilmu Pertanian Vol 7, No 1 (2022): AGROVITAL VOLUME 7, NOMOR 1, MEI 2022
Publisher : Universitas Al Asyariah Mandar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35329/agrovital.v7i1.2692

Abstract

Tujuan penelitian ini untuk mengetahui pengaruh suplementasi ekstrak daun kelor (Moringa oleifera) terhadap kualitas makroskopis spermatozoa ayam kampung. Penelitian ini menggunakan 15 ekor ayam kampung jantan berumur + 1 tahun dibagi secara acak dalam 3 kelompok perlakuan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari 3 perlakuan dan 5 ulangan. Sebelum diberi perlakuan, seluruh ayam diadaptasikan selama 1 minggu dan hanya diberi pakan komersil dan air minum. Perlakuan diberikan secara oral pada pagi hari selama 45 hari dan terdiri dari P1 : Ekstrak Daun Kelor 3 ml, P2 : Ekstrak Daun Kelor 6 ml, dan P3 : Ekstrak Daun Kelor 9 ml. Setelah dipelihara dan diberi perlakuan selama 45 hari, dilakukan penampungan sperma. Teknik penampungan sperma pada ayam dilakukan dengan menggunakan metode massage (metode pemijatan) pada bagian punggung ayam. Sperma yang telah ditampung kemudian diperiksa kualitasnya di Laboratorium. Parameter yang diukur yaitu kualitas sperma secara makroskopis meliputi volume, pH, warna, konsistensi, dan bau sperma. Hasil penelitian menunjukkan bahwa pemberian suplementasi ekstrak daun kelor tidak berbeda nyata terhadap volume, pH, warna, konsistensi, dan bau sperma (P>0,05) tetapi seluruh kualitas sperma yang diperoleh setelah pemberian  ekstrak daun kelor termasuk dalam kategori yang normal dan baik.
Potensi Energi Terbarukan dari Biogas Limbah Ternak Ruminansia Di Kabupaten Sidrap Nurul Purnomo; Yusriadi Yusriadi
Jurnal Sains dan Teknologi Industri Peternakan Vol 3 No 1 (2023): Februari 2023
Publisher : Program Studi Peternakan Universitas Muhammadiyah Sidenreng Rappang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55678/jstip.v3i1.621

Abstract

The increase in the population of ruminants has resulted in an increase in the production of livestock waste which has the potential to be a source of environmental pollutants as well as a potential biogas producer. The study of the biogas potential of ruminant livestock waste in Sidrap Regency was carried out using secondary data from BPS. Biogas potential is calculated by multiplying the ruminant population with the potential for livestock waste production, solid matter content, volatile matter content, and volatile matter biogas production in one year. The potential energy for biogas propulsion from ruminant livestock waste is calculated using the multiplication of the potential for biogas production per year with the methane content of biogas and the energy value of methane gas. The biogas equivalence of LPG gas is calculated by multiplying the biogas potential of ruminant livestock waste by the equivalent per m3 of biogas with LPG. The results of the study show that the biogas potential of ruminant slag waste in Sidrap Regency is 1,071,508.23 m3/year. The potential driving energy of biogas from ruminant livestock waste in Sidrap Regency is 5,400,401.49 kWh/year. The biogas potential of ruminant livestock waste in Sidrap Regency is equivalent to 492.893.79 kg/year LPG. Based on the results of the study, it can be concluded that the potential for renewable energy biogas from ruminant livestock waste in Sidrap Regency is very large.
POTENSI LIMBAH TERNAK RUMINANSIA DI KABUPATEN SIDRAP Nurul Purnomo; Armayani M.
Jurnal Sains dan Teknologi Industri Peternakan Vol 1 No 1 (2021): Februari 2021
Publisher : Program Studi Peternakan Universitas Muhammadiyah Sidenreng Rappang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55678/jstip.v1i1.196

Abstract

Increasing livestock population has an impact on increasing livestock waste production. Livestock waste can be a source of environmental pollution but can also be processed into organic fertilizer and biogas. But until now there has been no research into waste production and the distribution of ruminant livestock waste production in Sidrap Regency. This study aims to determine the production of ruminant livestock waste and the distribution of livestock waste production in Sidrap Regency. The results showed that ruminant livestock waste production in Sidrap District reached 204,903 tons / year consisting of 235,797 tons / year of cattle livestock waste, 17,290 tons / year of buffalo and 1,816 tons / year of goats. Livestock waste production is spread throughout the entire district area. The highest livestock waste production in Pituriase District, amounting to 84,274 tons / year, then Watang Pulu District 78,235 tons / year, Panca Lautang 27,186 tons / year, Kulo 12,164 tons / year, Pitu Riawa 10.765 tons / year, Maritengae 9,867 tons / year, Panca Lautang 27,186 tons / year, Kulo 12,164 tons / year, Pitu Riawa 10.765 tons / year, Maritengae 9,867 tons / year, Panca Lautang Rijang 9,120 tons / year, Watang Sidenreng 8,407 tons / year, Tellu Limpoe 7,624 tons / year, Two Pitue 5,023 tons / year and Baranti 2,238 tons / year. Based on the results of the study, it can be concluded that the production of livestock waste in Sidrap Regency reaches 204,903 tons / year and is spread unevenly throughout the district.