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EVOLUSI, SPESIASI, DAN HIBRIDISASI PADA BEBERAPA ANGGOTA SAPINDACEAE Djuita, Nina Ratna
BIOEDUKASI Vol 5, No 2 (2012): BIOEDUKASI
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

ABSTRACT-Evolution in Sapindaceae have been running for a long time, as evidenced by the fossil of Sapindopsis encountered in the mid Cretaceous.  Based on its characteristics, the genus is thought to live in stressful environments.  Sapindaceae has the habitus of shrubs, trees, climbers, and the vine. Speciation in Sapindaceae can be found in genus Acer, while members of Sapindaceae which had hybridization can be found in genus Aesculus.   Keyword: evolution, speciation, hybridization, Sapindaceae
SITOLOGI DAN TIPE REPRODUKSI Pteris multifida Poir. (PTERIDACEAE) Hastuti, Diah Virsa; Praptosuwiryo, Titien Ngatinem; Djuita, Nina Ratna
Buletin Kebun Raya Vol 14, No 1 (2011): Buletin Kebun Raya Vol. 14 (1) Januari 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Cytological observations have been carried out by several researchers in South China, Japan, Ceylon, Hongkong, Himalaya and Java. The results from various regions showed that P. multifida had a fairly high genetic variation, namely diploid, triploid and tetraploid types. Therefore cytological and reproductive study on P. multifida from its geographical distribution areas need to be done. The objectives of this research were to observe the somatic chromosome number and reproductive type of P. multifida, and to recognize the relationship between morphological variation and its ploidy level as well as stomatal index. Plants were collected from several localities in Bogor. Ploidy level was determined by observing somatic chromosome number by using squash methods. Reproductive type was determined by counting spore number in each sporangium. Morphological characters examined were the stipe, lamina, pinnae, veins, and indusium. Observations of stomata and epidermis of leaves were done by making an incision paradermal leaf. Pteris multifida has two ploidy level and reproduction type, namely apogamous triploid (2n = 87) and sexual tetraploid (2n = 116). The two ploidy levels could not be distinguished based on morphological characteristics, but they can be differentiated by their epidermal cells number. Apogamous triploid has fewer number of epidermal cells, the sexual tetraploid has a greater number of epidermis.
SITOLOGI DAN TIPE REPRODUKSI Pteris multifida Poir. (PTERIDACEAE) Hastuti, Diah Virsa; Praptosuwiryo, Titien Ngatinem; Djuita, Nina Ratna
Buletin Kebun Raya Vol 14, No 1 (2011): Buletin Kebun Raya Vol. 14 (1) January 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Abstract

Cytological observations have been carried out by several researchers in South China, Japan, Ceylon, Hongkong, Himalaya and Java. The results from various regions showed that P. multifida had a fairly high genetic variation, namely diploid, triploid and tetraploid types. Therefore cytological and reproductive study on P. multifida from its geographical distribution areas need to be done. The objectives of this research were to observe the somatic chromosome number and reproductive type of P. multifida, and to recognize the relationship between morphological variation and its ploidy level as well as stomatal index. Plants were collected from several localities in Bogor. Ploidy level was determined by observing somatic chromosome number by using squash methods. Reproductive type was determined by counting spore number in each sporangium. Morphological characters examined were the stipe, lamina, pinnae, veins, and indusium. Observations of stomata and epidermis of leaves were done by making an incision paradermal leaf. Pteris multifida has two ploidy level and reproduction type, namely apogamous triploid (2n = 87) and sexual tetraploid (2n = 116). The two ploidy levels could not be distinguished based on morphological characteristics, but they can be differentiated by their epidermal cells number. Apogamous triploid has fewer number of epidermal cells, the sexual tetraploid has a greater number of epidermis.
DISTRIBUSI KAPULASAN (NEPHELIUM RAMBOUTAN-AKE (LABILL.) LEENH.) DI PULAU JAWA DAN HUBUNGAN KEKERABATAN MORFOLOGINYA Nina Ratna Djuita; Alex Hartana; Tatik Chikmawati; Dorly Dorly
Floribunda Vol. 5 No. 4 (2016)
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (480.262 KB) | DOI: 10.32556/floribunda.v5i4.2016.133

Abstract

Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati & Dorly. 2016. Distribution of pulasan [Nephelium  ramboutan-ake (Labill.) Leenh] in Java and Relationship of Their Morphology. Floribunda 5(4): 129–138. — Distribution of pulasan in Java is not known yet. The purposes of this study were to provide information about the locations of pulasan trees in Java, and to analyze relationship of this species based on morphological characters. Pulasan exploration was carried out in four provinces in Java encompassed West Java, Central Java, East Java, and Yogyakarta Special Region. Morphological characteristics of pulasan were observed, then the relationships were analyze using NTSys pc 2.02 program.  Result showed that most of pulasan located in West Java, especially in the area of Bogor.  Based on the dendrogram, it was known that the use of ve-getative dan generative characters could distinguish males and hermaphrodite pulasan trees, while the use of vegetative characters only, could not differentiate the type of pulasan trees. Pulasan trees from different areas can be grouped in the same cluster because of the similarity of their morphological traits.Keywords: Kapulasan, morphological characters, dendrogram, Sapindaceae. Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati & Dorly. 2016. Distribusi Kapulasan [Nephelium ramboutan-ake (Labill.) Leenh.] di Pulau Jawa dan Hubungan Kekerabatan Morfologinya. Floribunda 5(4): 129–138. — Persebaran kapulasan di Pulau Jawa belum diketahui dengan pasti.  Penelitian ini bertujuan memberikan informasi tentang lokasi-lokasi yang masih mempunyai pohon kapulasan di Pulau Jawa, dan menganalisis hubungan kekerabatannya berdasarkan ciri morfologi. Eksplorasi kapulasan dilakukan di empat provinsi di Pulau Jawa yaitu Jawa Barat, Jawa Tengah, Jawa Timur, dan Daerah Istimewa Yogyakarta. Tanaman hasil eksplorasi diamati ciri morfologinya, kemudian dianalisis hubungan kekerabatannya dengan menggunakan program NTSys pc 2.02.  Hasil penelitian menunjukkan bahwa sebagian besar kapulasan terdapat di Jawa Barat terutama di daerah Bogor. Berdasarkan dendrogram yang dihasilkan, diketahui bahwa penggunaan ciri  vegetatif dan generatif dapat membedakan kapulasan jantan dan hermafrodit, sedangkan penggunaan ciri  vegetatif saja, tidak dapat membedakan kedua tipe tanaman tersebut. Tanaman kapulasan dari daerah yang berbeda dapat mengelompok dalam satu grup yang sama karena adanya keserupaan dalam ciri-ciri morfologinya.Kata kunci: Kapulasan, karakter morfologi, dendrogram, Sapindaceae. 
EVOLUSI, SPESIASI, DAN HIBRIDISASI PADA BEBERAPA ANGGOTA SAPINDACEAE Nina Ratna Djuita
Bioedukasi: Jurnal Pendidikan Biologi Vol 5, No 2 (2012): BIOEDUKASI: Jurnal Pendidikan Biologi
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/bioedukasi-uns.v5i2.3926

Abstract

Evolution in Sapindaceae have been running for a long time, as evidenced by the fossil of Sapindopsis encountered in the mid Cretaceous. Based on its characteris­tics, the genus is thought to live in stressful environments. Sapindaceae has the habitus of shrubs, trees, climbers, and the vine. Speciation in Sapindaceae can be found in genus Acor, while members of Sapindaceae which had hybridization can be found in genus AesculusEvolution in Sapindaceae have been running for a long time, as evidencedby the fossil of Sapindopsis encountered in the mid Cretaceous. Based on its characteristics, the genus is thought to live in stressful environments. Sapindaceae has the habitusof shrubs, trees, climbers, and the vine. Speciation in Sapindaceae can be found in genusAcer, while members of Sapindaceae which had hybridization can be found in genus Aesculus.
Kultur in vitro pisang (Musa paradisiaca L.) cv. Kepok Merah untuk mikropropagasi cepat [In vitro culture of banana (Musa paradisiaca) cv. Kepok Merah for rapid micropropagation] Efah FITRAMALA; Eva KHAERUNNISA; Nina Ratna Djuita Ratna DJUITA; Hadi SUNARSO; Diah RATNADEWI
E-Journal Menara Perkebunan Vol 84, No 2 (2016): Desember 2016
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (629.72 KB) | DOI: 10.22302/iribb.jur.mp.v84i2.221

Abstract

 Banana (Musa paradisiaca L) cv. Kepok Merah has a high commercial value as it is used in food industries such as banana chip. Besides, Kepok Merah contains high B-complex vitamins that serve in energy metabolism and  in the development of infant brain. The establishment of industrial plantations of this plant has been restricted by the lack of planting materials. This research aimed at ameliorating the capacity of plantlets multiplication up to rooting of this banana in a rapid way through in vitro multiplication techniques. Murashige and Skoog (MS) and Woody Plant (WP) media were used as the basic media. For the initiation stage, the media was fortified with 0.2 mg/L IAA and two levels of BA at 3 and 5 mg/L.  For shoot multiplication, the concentrations of IAA as well as BA were increased. For rooting, 1 mg/L NAA or IBA was applied. The observations demonstrated that for shoots initiation, both basic media performed good results when enriched with 0.2 mg/L IAA and 5 mg/L BA. The highest rate of shoots multiplication at 6 – 17 shoots per explant, was obtained on MS medium added with 0.5 mg/L IAA and 5 mg/L BA.  NAA at 1 mg/L in MS medium produced more rooted plantlets, 3 – 16 roots per plantlet, than those of other treatments. [Keywords: Musa paradisiaca cv. Kepok Merah, in vitro micropropagation, scalps.]AbstrakPisang (Musa paradisiaca L.) kultivar Kepok Merah memiliki nilai komersial yang cukup tinggi yaitu sebagai bahan dalam industri pembuatan keripik pisang. Selain itu, pisang Kepok Merah memiliki kandungan vitamin B kompleks cukup tinggi untuk membantu produksi energi dan pembentukan sel-sel otak pada bayi. Pertanaman pisang ini dalam skala industri terkendala oleh kurangnya ketersediaan sumber benih. Teknik kultur jaringan diharapkan dapat menghasilkan benih secara massal dalam waktu yang relatif singkat. Tujuan dari penelitian ini adalah meningkatkan keberhasilan multiplikasi tunas in vitro hingga pengakaran tanaman pisang Kepok Merah secara cepat. Pada tahap inisiasi tunas digunakan media dasar Murashige and Skoog (MS) dan Woody Plant (WP), ke dalam media dasar tersebut ditambahkan IAA 0,2 mg/L dan 2 taraf BA yaitu 3 dan 5 mg/L. Multiplikasi tunas dilakukan pada media dasar yang sama namun dengan taraf konsentrasi IAA serta BA yang ditingkatkan. Tahap perakaran menggunakan media dasar MS dan WP dengan auksin NAA 1 mg/L atau IBA 1 mg/L. Hasil penelitian menunjukkan bahwa untuk inisiasi tunas, media MS dan WP yang diperkaya dengan IAA 0,2 mg/L dan BA 5 mg/L   sama baiknya. Untuk  multiplikasi  tunas,   media  MS dengan IAA 0,5 mg/L   yang dikombinasikan dengan BA 5 mg/L   memberikan jumlah tunas paling banyak, yaitu 6 – 17 tunas per eksplan, dan pertumbuhannyapun lebih baik. Pemberian  NAA 1 mg/L pada media MS dapat memberikan lebih banyak tunas yang berakar, dengan jumlah akar 3 – 16 per planlet.  [Kata kunci: Musa paradisiaca cv. Kepok Merah, mikropropagasi in vitro, nodul meristematik.]
Characteristics and Ideotype Formulation of Pulasan (Nephelium ramboutan-ake) Fruit Landrace from West Java, Indonesia Nina Ratna Djuita; Alex Hartana; Tatik Chikmawati; Dorly Dorly
Makara Journal of Science Vol 21, No 2 (2017): June
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

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Abstract

Pulasan (Nephelium ramboutan-ake) is a unique tropical fruit, but its morphological variations have not been well characterized. This study was conducted to obtain data on the characteristics of pulasan landrace in West Java, Indonesia, and to formulate the ideotype of pulasan with superior characteristics, such as high fruit weight, sweet taste, and thick and easily peelable aril from the seed. Based on the descriptors of rambutan, 61 fruit variants were observed. The results showed that the average fruit weight was 46.0 g, with the highest at 103.5 g and the lowest at 23.1 g. The °Brix level variation of pulasan fruit was 16.8–29.6, and its vitamin C content was 14.0–24.0 mg/100 g. Five variants with superior fruit characters were identified among the sample collections used in the study.
Evaluation of Pulasan (Nephelium ramboutan-ake) Genetic Diversity in Bogor, West Java, Using Microsatellite Markers Puhili, Amelia Luisyane; Chikmawati, Tatik; Djuita, Nina Ratna
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.09

Abstract

Pulasan (Nephelium ramboutan-ake (Labill.) Leenh) fruit is highly similar to rambutan rapiah (Nephelium lappaceum) fruit with ovate shape, sweet and sour fresh taste, but it has a thick rind. The diversity of pulasan is little informed including in Bogor. The objective of this study was to analyze the genetic diversity of pulasan from Bogor revealed by microsatellite marker. The DNA of 63 individuals from 10 populations of pulasan were extracted using CTAB method and amplified using two primer sets, LMLY6 (GA)9(CA)2(GA)4 dan LMLY12 (CT)11. DNA amplification product was visualized and arranged in a matrix of binary data then analyzed the value of the number of different alleles (Na), the number of effective alleles (Ne), Shannon information index (I), heterozygosity (He), and the percentage of polymorphism (PLP). The results of the analysis showed the highest genetic diversity was found in North Bogor (He=0.313). The genetic diversity within a population (61%) was higher than that among populations (39%). A dendrogram was constructed using the Unweighted Pair Group Method with arithmetic Mean (UPGMA). The similarity index ranged from 52 to 100% that means there are close relationships among individuals. Cluster analyses grouped some individuals originated from different locations in the same group. The levels of heterozygosity within a population was determined by the history of each individual in a population.
PENGGUNAAN ANALISIS PEUBAH GANDA DALAM TAKSONOMI NUMERIK : CONTOH KASUS 2 KULTIVAR KEDELAI DAN KETURUNANNYA (F2) Nina Ratna Djuita; Muhammad Jusuf
Floribunda Vol. 3 No. 1-2 (2006)
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4633.509 KB) | DOI: 10.32556/floribunda.v3i1-2.2006.66

Abstract

Nina Ratna Djuita & Muhammad Jusuf. 2006. The use of Multivariate Analysis in Numerical Taxonomy : Case of Two, Soybean Cultivars and Their Generation (F2). Floribunda 3(1): 10-18. --- Multivariate analysis was applied to the morphological data of Slamet and Nokon Sawon cultivars and their generation (F2). This research was intended to compare multivariate analysis namely principal component analysis and disciminant analysis to two cultivars above and their generation. The first analysis resulted 2 and 7 principal components of covariance and correlation matrix for original data while data transformed revealed 5 and 8 principal components of covariance and correlation matrix respectively. Based on discriminant analysis, Nokon and Sawon’s cultivar can be successfully classified as a single group different from the rest.
MORPHOLOGICAL VARIATION OF KEDONDONG (SPONDIAS DULCIS PARKINSON) IN CENTRAL PART OF SUMATRA Ibna Hayati; Alex Hartana; Nina Ratna Djuita; Nunik Sri Ariyanti
Floribunda Vol. 6 No. 8 (2022)
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32556/floribunda.v6i8.2022.375

Abstract

Ibna Hayati, Alex Hartana, Nina Ratna Djuita & Nunik Sri Ariyanti. 2022. Morphological Variation of Kedondong  (Spondias dulcis Parkinson) in Central Part of  Sumatra. Floribunda 6(8): 315–323 — Kedondong (Spondias dulcis Parkinson), is widely cultivated in the tropics and reported possibly native plant from Malesia and Pacific. Since a long time several regions in Indonesia has been known as producing area. Around 60s, Sumatra has reputable kedondong from sabang which has sweet flavour and large size. However information on the morphological variation of kedondong germplasm is still limited. The objective of this study was to describe and grouped morphological variation of kedondong germplasm in central part of Sumatra. The specimens were explored and collected from Riau, West Sumatra, and Jambi. Fifty morphological characters were observed and scored for grouping analysis using UPGMA methods. Some characteristics of kedondong from central part of Sumatra are very varied. The kedondong specimens in the cluster analysis grouped into two main groups A and B based on the color of the mature fruit, but these characteristics may not qualify to be used as standard criteria for evaluating cultivated variety because within the group there are still variations in other characters, such as size and shape of the fruits. However based on fruit type, sweet kedondong tends to has oblong type.