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SITOLOGI DAN TIPE REPRODUKSI Pteris multifida Poir. (PTERIDACEAE) Hastuti, Diah Virsa; Praptosuwiryo, Titien Ngatinem; Djuita, Nina Ratna
Buletin Kebun Raya Vol 14, No 1 (2011): Buletin Kebun Raya Vol. 14 (1) Januari 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

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Cytological observations have been carried out by several researchers in South China, Japan, Ceylon, Hongkong, Himalaya and Java. The results from various regions showed that P. multifida had a fairly high genetic variation, namely diploid, triploid and tetraploid types. Therefore cytological and reproductive study on P. multifida from its geographical distribution areas need to be done. The objectives of this research were to observe the somatic chromosome number and reproductive type of P. multifida, and to recognize the relationship between morphological variation and its ploidy level as well as stomatal index. Plants were collected from several localities in Bogor. Ploidy level was determined by observing somatic chromosome number by using squash methods. Reproductive type was determined by counting spore number in each sporangium. Morphological characters examined were the stipe, lamina, pinnae, veins, and indusium. Observations of stomata and epidermis of leaves were done by making an incision paradermal leaf. Pteris multifida has two ploidy level and reproduction type, namely apogamous triploid (2n = 87) and sexual tetraploid (2n = 116). The two ploidy levels could not be distinguished based on morphological characteristics, but they can be differentiated by their epidermal cells number. Apogamous triploid has fewer number of epidermal cells, the sexual tetraploid has a greater number of epidermis.

SITOLOGI DAN TIPE REPRODUKSI Pteris multifida Poir. (PTERIDACEAE) Hastuti, Diah Virsa; Praptosuwiryo, Titien Ngatinem; Djuita, Nina Ratna
Buletin Kebun Raya Vol 14, No 1 (2011): Buletin Kebun Raya Vol. 14 (1) January 2011
Publisher : Center for Plant Conservation Bogor Botanic Garden, Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar

Cytological observations have been carried out by several researchers in South China, Japan, Ceylon, Hongkong, Himalaya and Java. The results from various regions showed that P. multifida had a fairly high genetic variation, namely diploid, triploid and tetraploid types. Therefore cytological and reproductive study on P. multifida from its geographical distribution areas need to be done. The objectives of this research were to observe the somatic chromosome number and reproductive type of P. multifida, and to recognize the relationship between morphological variation and its ploidy level as well as stomatal index. Plants were collected from several localities in Bogor. Ploidy level was determined by observing somatic chromosome number by using squash methods. Reproductive type was determined by counting spore number in each sporangium. Morphological characters examined were the stipe, lamina, pinnae, veins, and indusium. Observations of stomata and epidermis of leaves were done by making an incision paradermal leaf. Pteris multifida has two ploidy level and reproduction type, namely apogamous triploid (2n = 87) and sexual tetraploid (2n = 116). The two ploidy levels could not be distinguished based on morphological characteristics, but they can be differentiated by their epidermal cells number. Apogamous triploid has fewer number of epidermal cells, the sexual tetraploid has a greater number of epidermis.

EVOLUSI, SPESIASI, DAN HIBRIDISASI PADA BEBERAPA ANGGOTA SAPINDACEAE Djuita, Nina Ratna
BIOEDUKASI Vol 5, No 2 (2012): BIOEDUKASI
Publisher : Prodi Pendidikan Biologi FKIP UNS

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ABSTRACT-Evolution in Sapindaceae have been running for a long time, as evidenced by the fossil of Sapindopsis encountered in the mid Cretaceous. Based on its characteristics, the genus is thought to live in stressful environments. Sapindaceae has the habitus of shrubs, trees, climbers, and the vine. Speciation in Sapindaceae can be found in genus Acer, while members of Sapindaceae which had hybridization can be found in genus Aesculus. Keyword: evolution, speciation, hybridization, Sapindaceae

Evaluation of Pulasan (Nephelium ramboutan-ake) Genetic Diversity in Bogor, West Java, Using Microsatellite Markers Puhili, Amelia Luisyane; Chikmawati, Tatik; Djuita, Nina Ratna
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.09

Pulasan (Nephelium ramboutan-ake (Labill.) Leenh) fruit is highly similar to rambutan rapiah (Nephelium lappaceum) fruit with ovate shape, sweet and sour fresh taste, but it has a thick rind. The diversity of pulasan is little informed including in Bogor. The objective of this study was to analyze the genetic diversity of pulasan from Bogor revealed by microsatellite marker. The DNA of 63 individuals from 10 populations of pulasan were extracted using CTAB method and amplified using two primer sets, LMLY6 (GA)9(CA)2(GA)4 dan LMLY12 (CT)11. DNA amplification product was visualized and arranged in a matrix of binary data then analyzed the value of the number of different alleles (Na), the number of effective alleles (Ne), Shannon information index (I), heterozygosity (He), and the percentage of polymorphism (PLP). The results of the analysis showed the highest genetic diversity was found in North Bogor (He=0.313). The genetic diversity within a population (61%) was higher than that among populations (39%). A dendrogram was constructed using the Unweighted Pair Group Method with arithmetic Mean (UPGMA). The similarity index ranged from 52 to 100% that means there are close relationships among individuals. Cluster analyses grouped some individuals originated from different locations in the same group. The levels of heterozygosity within a population was determined by the history of each individual in a population.

Identifikasi Tumbuhan Penghasil Polen dari Madu Lebah Tanpa Sengat di Belitung: Identification of Plants as Pollen Source in Honey of Stingless Bee Heterotrigona itama and Tetragonula laeviceps from Belitung Abyan Setya Priambudi; Rika Raffiudin; Nina Ratna Djuita
Jurnal Sumberdaya Hayati Vol. 7 No. 1 (2021)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.7.1.25-35

Stingless bees forage to the flower for nectar and at the same time, pollen also trapped in the body hairs of the bees. Mostly pollen was concentrated into the pollen basket and transferred to the pollen and honey pot in the stingless bee nest. Pollen is male gametophytes of seed plants which have various of shapes, sizes, and ornamentations. This palynology study was aimed to identify the plants of pollen source that were found in honey of stingless bee Heterotrigona itama and Tetragonula laeviceps in Belitung and to analyze characteristics of the pollen. Pollen was extracted from honey and conducted the acetolysis and identification of the pollen in honey. We found 11 and 19 plant species as pollen soirce in the honey of H. itama and T. laeviceps, respectively. Pollen from Macaranga tanarius (23.72%) and Cocos nucifera (22.53%) were the two most dominant species on the H. itama honey. Pollen from Ageratum conyzoides (16.36%) are most often found in T. laeviceps honey. The most common pollen in honey of H. itama and T. laeviceps were from Arecaceae with a total of three species. Pollen from plants with tree habitus is most commonly found in honey of both species of stingless bees.

Paku Epifit pada Gymnospermae di Kebun Raya Cibodas: Epiphytic Ferns on Gymnosperms at Cibodas Botanical Garde Rizkia Adhatirana; Nina Ratna Djuita; Sulistijorini Sulistijorini; Taufikurrahman Nasution
Jurnal Sumberdaya Hayati Vol. 7 No. 2 (2021)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.7.2.49-56

Epiphytic ferns can be found in host trees from the Angiosperm and Gymnosperm groups. Epiphytic ferns in Angiosperm plants host have been widely studied, but there is little known for Gymnosperm plants host. The aim of this study was to identify the species of epiphytic ferns in the Gymnosperm plants host at Cibodas Botanical Garden and to analyze the diversity of epiphytic ferns based on microclimate conditions and the surface texture of Gymnosperms plants host. Epiphytic ferns diversity data was obtained using purposive random sampling method. Factors that influence the occupancy of ferns are analyzed using Principal Component Analysis. Epiphytic ferns in Gymnosperm host at Cibodas Botanical Garden were identified as 18 species including 7 family. The most dominant species of epiphytic fern is Davallia denticulata (59.45%). Diversity of epiphytic fern on Gymnosperm at Cibodas Botanical Garden is moderate (H’ = 1.81).

VARIASI KARAKTER MORFOLOGI DAN AGRONOMI BUAH KAPULASAN (Nephelium ramboutan-ake) DARI BOGOR, JAWA BARAT Nina Ratna Djuita; Tatik Chikmawati; Alex Hartana
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 11, No 1 (2014): Prosiding Seminar Nasional XI Biologi
Publisher : Universitas Sebelas Maret

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Abstract- Kapulasan is a unique tropical fruit. Morphological and agronomical variation of the fruit has not been known. The aim of this study was to obtain some variants of fruit that have superior characteristics such as hight fruit weight, sweet and thick aril, and fleaking aril. Thirty fruit variants was observed their morphological and agronomical characters based on the descriptor of rambutan. The results showed that the average of fruit weight is 50.4 g, a collection from Gunung Batu village has the highest fruit weight (103.5 g), while a collection from Makam Bodas village has the lowest fruit weight (23.1 g). The oBrix variation of kapulasan fruit ranged from 18.4 - 29.6, while the content of vitamin C ranged 3.5 - 5.6 mg/100g. There are four variants with superior fruit. Keywords:kapulasan, Nephelium ramboutan-ake, morphological and agronomical character

Kultur in vitro pisang (Musa paradisiaca L.) cv. Kepok Merah untuk mikropropagasi cepat [In vitro culture of banana (Musa paradisiaca) cv. Kepok Merah for rapid micropropagation] Efah FITRAMALA; Eva KHAERUNNISA; Nina Ratna Djuita Ratna DJUITA; Hadi SUNARSO; Diah RATNADEWI
E-Journal Menara Perkebunan Vol 84, No 2 (2016): Desember 2016
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Banana (Musa paradisiaca L) cv. Kepok Merah has a high commercial value as it is used in food industries such as banana chip. Besides, Kepok Merah contains high B-complex vitamins that serve in energy metabolism and in the development of infant brain. The establishment of industrial plantations of this plant has been restricted by the lack of planting materials. This research aimed at ameliorating the capacity of plantlets multiplication up to rooting of this banana in a rapid way through in vitro multiplication techniques. Murashige and Skoog (MS) and Woody Plant (WP) media were used as the basic media. For the initiation stage, the media was fortified with 0.2 mg/L IAA and two levels of BA at 3 and 5 mg/L. For shoot multiplication, the concentrations of IAA as well as BA were increased. For rooting, 1 mg/L NAA or IBA was applied. The observations demonstrated that for shoots initiation, both basic media performed good results when enriched with 0.2 mg/L IAA and 5 mg/L BA. The highest rate of shoots multiplication at 6 – 17 shoots per explant, was obtained on MS medium added with 0.5 mg/L IAA and 5 mg/L BA. NAA at 1 mg/L in MS medium produced more rooted plantlets, 3 – 16 roots per plantlet, than those of other treatments. [Keywords: Musa paradisiaca cv. Kepok Merah, in vitro micropropagation, scalps.]AbstrakPisang (Musa paradisiaca L.) kultivar Kepok Merah memiliki nilai komersial yang cukup tinggi yaitu sebagai bahan dalam industri pembuatan keripik pisang. Selain itu, pisang Kepok Merah memiliki kandungan vitamin B kompleks cukup tinggi untuk membantu produksi energi dan pembentukan sel-sel otak pada bayi. Pertanaman pisang ini dalam skala industri terkendala oleh kurangnya ketersediaan sumber benih. Teknik kultur jaringan diharapkan dapat menghasilkan benih secara massal dalam waktu yang relatif singkat. Tujuan dari penelitian ini adalah meningkatkan keberhasilan multiplikasi tunas in vitro hingga pengakaran tanaman pisang Kepok Merah secara cepat. Pada tahap inisiasi tunas digunakan media dasar Murashige and Skoog (MS) dan Woody Plant (WP), ke dalam media dasar tersebut ditambahkan IAA 0,2 mg/L dan 2 taraf BA yaitu 3 dan 5 mg/L. Multiplikasi tunas dilakukan pada media dasar yang sama namun dengan taraf konsentrasi IAA serta BA yang ditingkatkan. Tahap perakaran menggunakan media dasar MS dan WP dengan auksin NAA 1 mg/L atau IBA 1 mg/L. Hasil penelitian menunjukkan bahwa untuk inisiasi tunas, media MS dan WP yang diperkaya dengan IAA 0,2 mg/L dan BA 5 mg/L sama baiknya. Untuk multiplikasi tunas, media MS dengan IAA 0,5 mg/L yang dikombinasikan dengan BA 5 mg/L memberikan jumlah tunas paling banyak, yaitu 6 – 17 tunas per eksplan, dan pertumbuhannyapun lebih baik. Pemberian NAA 1 mg/L pada media MS dapat memberikan lebih banyak tunas yang berakar, dengan jumlah akar 3 – 16 per planlet. [Kata kunci: Musa paradisiaca cv. Kepok Merah, mikropropagasi in vitro, nodul meristematik.]

KEBERAGAMAN GENETIK KERABAT RAMBUTAN LIAR (NEPHELIUM SPP.) DI KABUPATEN SANGGAU, KALIMANTAN BARAT BERDASARKAN MARKA SSR DAN ISSR Christyne SPLS Napitu; Tatik Chikmawati; Nina Ratna Djuita
Floribunda Vol. 5 No. 4 (2016)
Publisher : PTTI

Christyne SPLS Napitu, Tatik Chikmawati & Nina Ratna Djuita. 2016. Genetic Diversity of Wild Rambutans (Nephelium spp.) in Sanggau Regency, West Kalimantan Based on SSR and ISSR Markers. Floribunda 5(4): 115–125. — This study aimed to determine the genetic diversity of wild rambutans from Sanggau Regency (West Kalimantan) based on SSR and ISSR markers. Plant materials were collected from five subdistricts: Bonti, Jangkang, Parindu, Mukok and Kapuas, in Sanggau Regency. There were four species of wild rambutans with five vatieties, namely N. cuspidatum var. cuspidatum, N. cuspidatum var. eriopetalum, N. cuspidatum var. robustum, N. lappaceum var. lappaceum, N. lappaceum var. xanthioides, N. rubescens and N. uncinatum found in the research site. The highest genetic diversity from the samples based on SSR markers was found in Jangkang (He=0.27) and the highest genetic diversity based on ISSR was found in Bonti (He=0.18). Cluster analysis using Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method and coefficient Simple Matching (SM) based on SSR and ISSR showed that there were high similarity among species of wild rambutans in Sanggau Regency, West Kalimantan with similarity index ranged 0.5–1.0. Keywords: Genetic diversity, ISSR, Nephelium, SSR, wild rambutans. Christyne SPLS Napitu, Tatik Chikmawati & Nina Ratna Djuita. 2016. Keberagaman Genetik Kerabat Rambutan Liar (Nephelium spp.) di Kabupaten Sanggau, Kalimantan Barat Berdasarkan Marka SSR dan ISSR. Floribunda 5(4): 115–125. — Penelitian ini bertujuan mengetahui keberagaman genetik rambutan liar yang berasal dari Kabupaten Sanggau (Kalimantan Barat) berdasarkan marka SSR dan ISSR. Pengambilan sampel diperoleh dari 5 Kecamatan: Bonti, Jangkang, Parindu, Mukok dan Kapuas, di Kabupaten Sanggau. Empat jenis rambutan liar beserta lima varietasnya yaitu N. cuspidatum var. cuspidatum, N. cuspidatum var. eriopetalum, N. cuspidatum var. robustum, N. lappaceum var. lappaceum, N. lappaceum var. xanthioides, N. rubescens dan N. uncinatum ditemukan di lokasi penelitian. Keberagaman genetik dengan nilai heterozigositas tertinggi berdasarkan marka SSR terdapat di Jangkang (He=0,27) dan berdasarkan marka ISSR terdapat di Bonti (He=0,18). Hasil analisis kelompok menggunakan metode Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dan koefisien indeks similaritas Simple Matching (SM) berdasarkan data SSR dan ISSR menunjukkan adanya tingkat kemiripan yang tinggi antara jenis rambutan liar di Kabupaten Sanggau, Kalimantan Barat dengan nilai koefisien kemiripan 0,5–1,0.Kata kunci: Keberagaman genetik, ISSR, Nephelium, SSR, rambutan liar.

PENGGUNAAN ANALISIS PEUBAH GANDA DALAM TAKSONOMI NUMERIK : CONTOH KASUS 2 KULTIVAR KEDELAI DAN KETURUNANNYA (F2) Nina Ratna Djuita; Muhammad Jusuf
Floribunda Vol. 3 No. 1-2 (2006)
Publisher : PTTI

Nina Ratna Djuita & Muhammad Jusuf. 2006. The use of Multivariate Analysis in Numerical Taxonomy : Case of Two, Soybean Cultivars and Their Generation (F2). Floribunda 3(1): 10-18. --- Multivariate analysis was applied to the morphological data of Slamet and Nokon Sawon cultivars and their generation (F2). This research was intended to compare multivariate analysis namely principal component analysis and disciminant analysis to two cultivars above and their generation. The first analysis resulted 2 and 7 principal components of covariance and correlation matrix for original data while data transformed revealed 5 and 8 principal components of covariance and correlation matrix respectively. Based on discriminant analysis, Nokon and Sawon’s cultivar can be successfully classified as a single group different from the rest.

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