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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Bioedukasi Buletin Kebun Raya JSDH Journal of Tropical Biodiversity and Biotechnology Bioedukasi: Jurnal Pendidikan Biologi Menara Perkebunan Floribunda Proceeding Biology Education Conference Makara Journal of Science

Nina Ratna Djuita

IPB University

Author-ID : 255176

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health

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KEANEKARAGAMAN LUMUT EPIFIT PADA GYMNOSPERMAE DI KEBUN RAYA BOGOR Fibo Adhitya; Nunik Sri Ariyanti; Nina Ratna Djuita
Floribunda Vol. 4 No. 8 (2014)
Publisher : PTTI

Since researchs on diversity of epiphytic Bryophytes are more common observed on Angiospermae than those on Gymnospermae, this study was conducted to observe diversity of epiphytic Bryophytes on Gymnospermae in BBG. The Bryophytes samples were collected from Gymnosperms trees in Blok I which is Cycadaceae familly and those in Blok II which are the familly of Pinaceae, Araucariaceae, Gnetaceae dan Podocarpaceae. Only the trees have diameter ≥ 20 cm of trunk were sampled. Bryophytes were collected from the trunk base at 0–200 cm above the ground, at four cardinal direction (north, south, west, and east). In total 18 species of epiphytic Bryophytes were found, included 12 genera and 7 families. The family which had the highest number of species was Lejeuneaceae. The most common species on Gymnosperm trees in the Botanical Garden were Lejeunea tuberculosa and Octoblepharum albidum. Both of them were found at 6 trees of 13 total tree samples. The species were found more frequently at west (2,1%) and north (1,8%) part than east and south part of the host trees. They more frequently inhabit the trunk base on 0–100 cm than 100–200 cm.

DISTRIBUSI KAPULASAN (NEPHELIUM RAMBOUTAN-AKE (LABILL.) LEENH.) DI PULAU JAWA DAN HUBUNGAN KEKERABATAN MORFOLOGINYA Nina Ratna Djuita; Alex Hartana; Tatik Chikmawati; Dorly Dorly
Floribunda Vol. 5 No. 4 (2016)
Publisher : PTTI

Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati & Dorly. 2016. Distribution of pulasan [Nephelium ramboutan-ake (Labill.) Leenh] in Java and Relationship of Their Morphology. Floribunda 5(4): 129–138. — Distribution of pulasan in Java is not known yet. The purposes of this study were to provide information about the locations of pulasan trees in Java, and to analyze relationship of this species based on morphological characters. Pulasan exploration was carried out in four provinces in Java encompassed West Java, Central Java, East Java, and Yogyakarta Special Region. Morphological characteristics of pulasan were observed, then the relationships were analyze using NTSys pc 2.02 program. Result showed that most of pulasan located in West Java, especially in the area of Bogor. Based on the dendrogram, it was known that the use of ve-getative dan generative characters could distinguish males and hermaphrodite pulasan trees, while the use of vegetative characters only, could not differentiate the type of pulasan trees. Pulasan trees from different areas can be grouped in the same cluster because of the similarity of their morphological traits.Keywords: Kapulasan, morphological characters, dendrogram, Sapindaceae. Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati & Dorly. 2016. Distribusi Kapulasan [Nephelium ramboutan-ake (Labill.) Leenh.] di Pulau Jawa dan Hubungan Kekerabatan Morfologinya. Floribunda 5(4): 129–138. — Persebaran kapulasan di Pulau Jawa belum diketahui dengan pasti. Penelitian ini bertujuan memberikan informasi tentang lokasi-lokasi yang masih mempunyai pohon kapulasan di Pulau Jawa, dan menganalisis hubungan kekerabatannya berdasarkan ciri morfologi. Eksplorasi kapulasan dilakukan di empat provinsi di Pulau Jawa yaitu Jawa Barat, Jawa Tengah, Jawa Timur, dan Daerah Istimewa Yogyakarta. Tanaman hasil eksplorasi diamati ciri morfologinya, kemudian dianalisis hubungan kekerabatannya dengan menggunakan program NTSys pc 2.02. Hasil penelitian menunjukkan bahwa sebagian besar kapulasan terdapat di Jawa Barat terutama di daerah Bogor. Berdasarkan dendrogram yang dihasilkan, diketahui bahwa penggunaan ciri vegetatif dan generatif dapat membedakan kapulasan jantan dan hermafrodit, sedangkan penggunaan ciri vegetatif saja, tidak dapat membedakan kedua tipe tanaman tersebut. Tanaman kapulasan dari daerah yang berbeda dapat mengelompok dalam satu grup yang sama karena adanya keserupaan dalam ciri-ciri morfologinya.Kata kunci: Kapulasan, karakter morfologi, dendrogram, Sapindaceae.

ANATOMI DAUN RAMBUTAN (NEPHELIUM LAPPACEUM L.) DAN KERABATNYA Qothrunnada - Sungkar; Tatik - Chikmawati; Nina Ratna Djuita
Floribunda Vol. 5 No. 6 (2017)
Publisher : PTTI

Qothrunnada Sungkar, Tatik Chikmawati & Nina Ratna Djuita. 2017. Leaf Anatomy of Rambutan (Nephelium lappaceum L.) and Its Relatives. Floribunda 5(6): 192–199. — Rambutan belongs to the Sapindaceae family that has closely related to longan and lychee. The observation on leaf anatomy of rambutan, longan, and lychee was conducted to provide information about leaf anatomy of Sapindaceae’s members. The anatomical features of leaf paradermal and transversal sections were examinated on four varieties of rambutan namely binjai, rapiah, sikoneng, and aceh lengkeng; and two closely related families, longan and lychee. All rambutan cultivars and lychee had the same stomata type: cyclocytic, while stomata in longan was surrounded by six subsidiary cells. Epidermal cells of rambutan and lychee leaves have polygonal shape with flat side, whereas epidermal cell of longan has polygonal shapes with notched. Based on transversal sections, rambutan, longan, and lychee have bifacial type, but they are differed in the number of palisade layer and the shape of palisade cellls. Leaf anatomical characters could be used to distinguish between rambutan and its closed relatives, longan and lychee; but it could not be used to differentiate among rambutan’s cultivars.Keywords: longan, lychee, rambutan, Sapindaceae, stomata type.Qothrunnada Sungkar, Tatik Chikmawati & Nina Ratna Djuita. 2017. Anatomi Daun Rambutan (Nephelium lappaceum L.) dan Kerabatnya. Floribunda 5(6): 192–199. — Rambutan merupakan tumbuhan yang termasuk ke dalam suku Sapindaceae dan masih berkerabat dekat dengan lengkeng dan leci. Pengamatan tentang anatomi daun Sapindaceae masih jarang dilakukan, sehingga penelitian ini perlu dilakukan untuk memberikan informasi mengenai anatomi daun beberapa anggota Sapindaceae. Pengamatan ciri anatomi dari sayatan paradermal dan transversal dilakukan terhadap empat kultivar rambutan yaitu rambutan binjai, rapiah, sikoneng, dan aceh lengkeng, serta dua kerabat dekatnya yaitu lengkeng dan leci. Hasil sayatan paradermal memperlihatkan bahwa stomata tidak ditemukan di permukaan atas daun rambutan, lengkeng, dan leci. Stomata pada daun rambutan dan leci adalah stomata dengan tipe siklositik, sedangkan stomata pada lengkeng dikelilingi oleh enam sel tetangga. Epidermis pada daun rambutan dan leci berbentuk poligonal dengan sisi rata, sedangkan sel epidermis pada daun lengkeng berbentuk poligonal dengan sisi berlekuk. Sayatan transversal memperlihatkan bahwa daun rambutan, lengkeng, dan leci bertipe bifasial. Ketiga jenis berbeda pada jumlah lapisan palisade dan bentuk sel palisade. Ciri anatomi daun dapat digunakan untuk membedakan rambutan dari kerabat dekatnya, leci dan lengkeng; tetapi tidak dapat digunakan untuk membedakan antara kultivar rambutan.Kata kunci: lengkeng, leci, rambutan, Sapindaceae, tipe stomata.

KEANEKARAGAMAN GENETIK KAPULASAN [NEPHELIUM RAMBOUTAN-AKE (LABILL.) LEENH.] DI JAWA BERDASARKAN MARKA SSR DAN ISSR Nina Ratna Djuita; Alex Hartana; Tatik Chikmawati; Dorly Dorly
Floribunda Vol. 6 No. 4 (2020)
Publisher : PTTI

Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati, Dorly. 2020. Genetic Diversity of Pulasan [Nephelium ramboutan-ake (Labill.) Leenh.] of Java Based on SSR and ISSR Markers. Floribunda 6(4): 117–126. — Pulasan is one of the potential local fruits to be developed. This study aimed to analyze the genetic diversity of pulasan of Java using Simple Sequence Repeat (SSR) and Inter Simple Sequence Repeat (ISSR) markers and to obtain information whether primers of the markers could be used to distinguish male and her-maphrodite plants. The results showed that two primers in the SSR markers and seven primers in the ISSR markers produced polymorphic bands. The genomic DNA of the pulasan amplified with SSR markers produced bands 140–500 bp, while those from the ISSR markers were 150–1500 bp. The population of pulasan in Babakan Madang has the highest genetic diversity, while that of Patean is the lowest. Genetic variation of pulasan based on SSR and ISSR markers in the population and among populations have different compositions. Variation in the population is 72% while among the population is 28%. Primers of LML Y6 and LML Y12 from SSR markers and primers of ISSR 2, 3, 4, 5, 6, 8, 9 cannot be used to distinguish male and hermaphrodite pulasan plants. Nina Ratna Djuita, Alex Hartana, Tatik Chikmawati, Dorly. 2020. Keanekaragaman Genetik Kapulasan [Nephelium ramboutan-ake (Labill.) Leenh.] di Jawa Berdasarkan Marka SSR dan ISSR. Floribunda 6(4): 117–126. — Kapulasan merupakan salah satu buah lokal yang potensial untuk dikembangkan. Penelitian ini bertujuan untuk menganalisis keanekaragaman genetik kapulasan di Jawa dengan menggunakan marka Simple Sequence Repeat (SSR) dan Inter Simple Sequence Repeat (ISSR) serta untuk mendapatkan informasi apakah primer dari marka tersebut dapat dipakai untuk membedakan tumbuhan jantan dan hermafrodit. Hasil penelitian menunjukkan bahwa dua primer pada marka SSR dan tujuh primer pada marka ISSR menghasilkan pita polimorfik. DNA genom kapulasan yang diamplifikasi dengan marka SSR menghasilkan pita-pita dengan ukuran 110–500 bp, sedangkan dari marka ISSR berukuran 150–1500 bp. Populasi kapulasan di Babakan Madang mempunyai keanekaragaman genetik paling tinggi, sedangkan populasi di Patean paling rendah. Variasi genetik kapulasan berdasarkan marka SSR dan ISSR di dalam populasi dan di antara populasi mempunyai komposisi yang berbeda. Variasi di dalam populasi sebesar 72 % sedangkan di antara populasi sebesar 28%. Primer LML Y6 dan LML Y12 dari marka SSR dan primer ISSR 2, 3, 4, 5, 6, 8, 9 tidak dapat digunakan untuk membedakan tumbuhan kapulasan jantan dan hermafrodit.

DIVERSITY OF HORNWORT IN MOUNT SLAMET (CENTRAL JAVA) Arin Ulfiana Mubarokah Siagian; Nunik Sri Ariyanti; Nina Ratna Djuita
Floribunda Vol. 6 No. 7 (2021): Floribunda
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32556/floribunda.v6i7.2021.357

Recent more intensive explorations and collections of hornworts in Mt. Slamet indicated that six species can be recognized, namely Anthoceros punctatus, Folioceros fuciformis, Phaeoceros carolinianus, P. exiguus, Dendroceros difficilis, and Megaceros flagellaris. Since originally only one species D. difficilis was recorded from this area, this means that five species represent new records for the area, whereas P. exiguus is not only a new record for Java, but it is also a new record for Malesia.

IS THE FLOWER OF PULASAN (NEPHELIUM RAMBOUTAN-AKE) A PROTOGYNY OR PROTANDRY? Nina Ratna Djuita; Alex Hartana; Tatik Chikmawati; Dorly Dorly
Floribunda Vol. 6 No. 7 (2021): Floribunda
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32556/floribunda.v6i7.2021.359

Pulasan is an androdioecious plant that has both male and hermaphrodite flowers on separate plants. The objectives of this study were to obtain data about the anatomical structure of pulasan flower and to investigate whether the pistil and the stamens of pulasan flower reach maturity at different times. The anatomical observation was done on compound flowers taken from three male trees and three hermaphrodite trees. Two compound flowers that still in buds, about to bloom and fully bloom were picked from each tree. Flowers were observed in a cross and longitudinal section. The results showed that the sepals of pulasan flower comprised of the uniseriate epidermis and multilayered polyhedral parenchymal cells. Stamen development started from the anther followed by the formation of the filament. The pollen of hermaphrodite pulasan flowers reached maturation earlier than the pistil. The pistil development started from the expansion of meristem cells in the center of the flower and ends with the warp of the stigma.

VARIASI MIKROMORFOLOGI SISIK PAKU DI KEBUN TEH KAMPUNG CITALAHAB, BOGOR, JAWA BARAT Ibna Hayati; Lana Maulana; Afri Irawan; Nurul Aini; Tira Tahnia; Nina Ratna Djuita; Tatik Chikmawati; Nunik Sri Ariyanti
Floribunda Vol. 6 No. 8 (2022)
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32556/floribunda.v6i8.2022.363

Paku merupakan tumbuhan vaskular penghasil spora yang terdiri atas akar, rimpang dan ental. Bagian rimpang, stipe dan bagian muda dari helaian daun seringkali ditutupi oleh indumentum (sisik/rambut). Penelitian ini bertujuan untuk mengkarakterisasi mikromorfologi dari 18 jenis paku terpilih yang dijumpai di kawasan kebun teh Kampung Citalahab. Pengambilan sampel dilakukan dengan metode jelajah bebas. Karakter mikromorfologi diamati dan didokumentasikan menggunakan mikroskop. Hasil pengamatan disajikan dalam bentuk gambar dan dianalisis secara deskriptif. Hasil karakterisasi mikromorfologi menunjukkan variasi pada warna sisik dan bentuk sisik. Karakter mikromorfologi yang diamati dapat memperkaya bukti taksonomi setiap jenis paku.

MORPHOLOGICAL VARIATION OF KEDONDONG (SPONDIAS DULCIS PARKINSON) IN CENTRAL PART OF SUMATRA Ibna Hayati; Alex Hartana; Nina Ratna Djuita; Nunik Sri Ariyanti
Floribunda Vol. 6 No. 8 (2022)
Publisher : PTTI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32556/floribunda.v6i8.2022.375

Ibna Hayati, Alex Hartana, Nina Ratna Djuita & Nunik Sri Ariyanti. 2022. Morphological Variation of Kedondong (Spondias dulcis Parkinson) in Central Part of Sumatra. Floribunda 6(8): 315–323 — Kedondong (Spondias dulcis Parkinson), is widely cultivated in the tropics and reported possibly native plant from Malesia and Pacific. Since a long time several regions in Indonesia has been known as producing area. Around 60s, Sumatra has reputable kedondong from sabang which has sweet flavour and large size. However information on the morphological variation of kedondong germplasm is still limited. The objective of this study was to describe and grouped morphological variation of kedondong germplasm in central part of Sumatra. The specimens were explored and collected from Riau, West Sumatra, and Jambi. Fifty morphological characters were observed and scored for grouping analysis using UPGMA methods. Some characteristics of kedondong from central part of Sumatra are very varied. The kedondong specimens in the cluster analysis grouped into two main groups A and B based on the color of the mature fruit, but these characteristics may not qualify to be used as standard criteria for evaluating cultivated variety because within the group there are still variations in other characters, such as size and shape of the fruits. However based on fruit type, sweet kedondong tends to has oblong type.

EVOLUSI, SPESIASI, DAN HIBRIDISASI PADA BEBERAPA ANGGOTA SAPINDACEAE Nina Ratna Djuita
Bioedukasi: Jurnal Pendidikan Biologi Vol 5, No 2 (2012): BIOEDUKASI: Jurnal Pendidikan Biologi
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/bioedukasi-uns.v5i2.3926

Evolution in Sapindaceae have been running for a long time, as evidenced by the fossil of Sapindopsis encountered in the mid Cretaceous. Based on its characteristics, the genus is thought to live in stressful environments. Sapindaceae has the habitus of shrubs, trees, climbers, and the vine. Speciation in Sapindaceae can be found in genus Acor, while members of Sapindaceae which had hybridization can be found in genus AesculusEvolution in Sapindaceae have been running for a long time, as evidencedby the fossil of Sapindopsis encountered in the mid Cretaceous. Based on its characteristics, the genus is thought to live in stressful environments. Sapindaceae has the habitusof shrubs, trees, climbers, and the vine. Speciation in Sapindaceae can be found in genusAcer, while members of Sapindaceae which had hybridization can be found in genus Aesculus.

- Anatomi Daun Varietas Belimbing (Averrhoa carambola L.) Lokal di Taman Buah Mekarsari Bogor: Leaf Anatomy of Local Variety Starfruit (Averrhoa carambola L.) at Mekarsari Fruit Garden, Bogor Tila Mardhatillah; . Dorly; Nina Ratna Djuita
Jurnal Sumberdaya Hayati Vol. 8 No. 1 (2022)
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.8.1.27-33

Sweet star fruit (Averrhoa carambola L., belimbing manis) is one of Indonesia’s most popular fruit plants. Anatomically, the characters of the local sweet star fruit of Mekar Sari Fruit Garden are not widely known, so this study aims to observe the anatomical structure of the leaves. The materials used were star fruit leaves of varieties Demak Jingga, Demak Kapur, Demak Kunir, Welahan, and Wulan. The leaves of star fruit varieties were made microscopic preparations in paradermal incisions using the whole mount method and transverse incisions using the paraffin method. The observation result of the paradermal incision of sweet star fruit leaves showed that the type of epidermal cell wall is straight and shallow grooved. The leaf stomata of five sweet star fruit varieties were paracytic. The calculation of the index value, size and density of stomata varied between varieties. The highest stomata density and index were found in the Welahan variety. The results of the transverse incision of sweet starfruit leaves showed that the five local sweet star fruit varieties have mesophyll tissue of the dorsiventral type. Wulan variety has leaves and mesophyll tissue which tends to be thicker than other varieties.

Co-Authors Abyan Setya Priambudi Afri Irawan ALEX HARTANA Alex Hartana Alex Hartana Alex Hartana Alex Hartana Alex Hartana Alex Hartana Amelia Luisyane Puhili Arin Ulfiana Mubarokah Siagian Christyne SPLS Napitu Diah Ratnadewi Diah Virsa Hastuti Dianti Mayadika Putri Dorly Dorly Dorly, Efah FITRAMALA Eva KHAERUNNISA Fibo Adhitya Hadi SUNARSO Ibna Hayati Lana Maulana Muhammad Jusuf NUNIK SRI ARIYANTI Nurul Aini Puhili, Amelia Luisyane Qothrunnada - Sungkar RIKA RAFFIUDIN Rizkia Adhatirana Siti Halimatussadiah Sulistijorini Sulistijorini Tatik - Chikmawati Tatik Chikmawati TATIK CHIKMAWATI Tatik Chikmawati Chikmawati Taufikurrahman Nasution Tila Mardhatillah Tira Tahnia Titien Ngatinem Praptosuwiryo

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